| purification and characterization of a corynebacterium ulcerans bacteriocin (ulceracin 378). | corynebacterium ulcerans strain 378 produces a bacteriocin (ulceracin 378) and a toxin when grown on semi-solid medium. ulceracin 378 was purified 360-fold by dialysis and chromatography on deae-cellulose and sephadex g-200. on the basis of ultrogel aca22 gel filtration its molecular weight was about 900 000. it could be dissociated by 2-mercaptoethanol and sodium dodecyl sulphate into smaller subunits of 25 000. the bactericidal activity was associated with this subunit which contained no carbo ... | 1985 | 3921657 |
| two distinct toxic proteins in corynebacterium ulcerans. | | 1974 | 4370843 |
| infections caused by nondiphtheria corynebacteria. | after decades of confusion about their microbiologic classification and clinical significance, the nondiphtheria corynebacteria have emerged as important pathogens. although isolation of these organisms may represent contamination with skin flora, several species, including corynebacterium ulcerans, corynebacterium pseudotuberculosis (corynebacterium ovis), corynebacterium haemolyticum, corynebacterium pseudodiptheriticum, corynebacterium equi, corynebacterium bovis, corynebacterium xerosis, and ... | 1982 | 6760340 |
| purification and characterization of some enzymatic properties of neuraminidase from corynebacterium ulcerans. | neuraminidase of corynebacterium-ulcerans was purified by affinity chromatography using immobilized colominic acid preparations. neuraminidase appears to be a thermolabile protein, molecular mass 70 000 da. the ph optimum of 5.5 is independent of the substrate used; the optimal temperature is 37 degrees c, the michaelis constant towards n-acetylneuraminosyllactose is 5.2 x 10(-4) m. ca2+ and ba2+ activated the enzyme, but zn2+, fe2+, and chelating agent edta were inhibitory. in our experiments t ... | 1981 | 6796488 |
| proof for the lack of diphtheria toxin in culture filtrates of corynebacterium ulcerans strain atcc 9015. | extensive efforts to identify the diphtheria toxin in culture filtrates of c. ulcerans strain atcc 9015 was unsuccessful. the culture filtrates did contain a component (called d-antigen) which formed a precipitin line with commercial diphtheria antitoxin. this component was purified by immunosorbent techniques using immobilized preparations of diphtheria antitoxin. it was shown, that d-antigen has a molecular weight of 84000, is not toxic for guinea-pigs, and has nothing in common with purified ... | 1981 | 6797150 |
| [corynebacterium ulcerans and its biological properties]. | | 1980 | 7004030 |
| purification and some properties of exotoxin from corynebacterium ulcerans strain atcc 9015. | corynebacterium ulcerans strain atcc 9015 develops during growth in environmental media an exotoxin which is lethal for guinea-pigs. the exotoxin has been purified by ion-exchange chromatography on deae-sephadex a-50, affinity chromatography using immunosorbent techniques, and gel-filtration through sephadex g-75. the exotoxin is of a protein nature and consists perhaps of two subunits. the molecular weights of the exotoxin and its subunits as estimated by sds-page were 40 000, 25 000, and 15 00 ... | 1981 | 7198352 |
| comparison of phenotypic and genotypic methods for detection of diphtheria toxin among isolates of pathogenic corynebacteria. | we have compared molecular, immunochemical, and cytotoxic assays for the detection of diphtheria toxin from 55 isolates of corynebacterium diphtheriae and corynebacterium ulcerans originally isolated in five different countries. the suitabilities and accuracies of these assays for the laboratory diagnosis of diphtheria were compared and evaluated against the "gold standard" in vivo methods. the in vivo and vero cell cytotoxicity assays were accurate in their abilities to detect diphtheria toxin ... | 1998 | 9774560 |
| isolation and characterization of corynebacterium ulcerans from cephalic implants in macaques. | to determine the prevalence of colonization by corynebacterium ulcerans, we cultured samples from the cephalic implant-skin margin and pharynx of 26 rhesus macaques and one pig-tailed macaque. all but one of the samples from the cephalic implants yielded a mixed population of bacteria. c. ulcerans grew from the cephalic implants in 56% and from the pharynx in 3% of the implanted animals. we screened nine of these isolates for diphtheria toxin (dt) and phospholipase d (pld). polymerase chain reac ... | 2000 | 11099137 |
| pigs as source for toxigenic corynebacterium ulcerans. | | 2009 | 19751602 |