| avian astroviruses. | as poultry becomes more important in the world economy, it is increasingly important to fully understand the mechanisms of disease and poor production that affect the industry. to more accurately and reasonably treat these diseases, a more sophisticated understanding of interrelatedness is required. this review focuses on avian astroviruses (aastvs), in particular the recent advances in our understanding of aastv molecular biology, and also history, diagnosis, treatment and control. the known aa ... | 2002 | 12396344 |
| development of antigen-capture enzyme-linked immunosorbent assay and rt-pcr for detection of turkey astroviruses. | turkey astrovirus (tastv) is an important agent of poult enteritis. the diagnosis of astroviruses has been dependent mainly on electron microscopy (em) or immune em (iem). to develop other simple, rapid, and reliable diagnostic assays, two antigen-capture enzyme-linked immunosorbent assays (ac-elisas), polyclonal ac-elisa and monoclonal ac-elisa, were developed in this study. monoplex and multiplex reverse transcription-polymerase chain reactions (rt-pcrs) were also developed using nondegenerate ... | 2005 | 16094820 |
| development of a multiplex reverse transcription-polymerase chain reaction diagnostic test specific for turkey astrovirus and coronavirus. | a multiplex reverse transcription-polymerase chain reaction (rt-pcr) assay was developed for the simultaneous detection of two enteric viruses of poultry: turkey enteric coronavirus (tcv) and turkey astrovirus (tastv). pcr primers were designed to conserved regions within the nucleocapsid gene of tcv and to the polymerase gene of tastv-2. the primer pairs were successfully used in a multiplex rt-pcr to detect nucleic acid of tastv-2 and tcv. the test was optimized for use with intestines/feces f ... | 2004 | 15529975 |