studies on tyzzer's disease: transplacental transmission of bacillus piliformis in rats.clinically healthy rats with antibodies to bacillus piliformis were given prednisolone in the last week of pregnancy. b. piliformis was demonstrated in the livers of their offspring. none of the dams or the young rats showed clinical signs of disease. antibodies to b. piliformis were found in the young rats at birth, and presisted for several months. the importance of potential transplacental infections when attempting to establish colonies free from b. piliformis in discussed.1979374861
studies on tyzzer's disease: acquired immunity against infection and activation of infection by immunosuppressive treatment.the correlation between serum antibody titre and resistance to challenge infection with bacillus piliformis was studied in naturally infected mice, in experimentally infected but recovered mice, and in mice treated with antigen prepared from infected livers. irrespective of the way in which the antibodies were acquired resistance to infection was found to be related to the immunofluorescence antibody titre found. experimentally infected but recovered mice, as well as rats with persistent antibod ...1979384085
cytotoxicity of bacillus isolates of b. piliformis, the agent of tyzzer's disease, obtained from various host species, were examined for cytotoxic activity by incubating culture filtrates on brl 3a rat-hepatocyte and 3t3 mouse-fibroblast cell lines. one isolate exhibited cytopathic effects on brl 3a cells, but not on 3t3 cells. three other isolates were strongly cytotoxic for 3t3 cells but only slightly so for brl 3a cells. the remaining three isolates showed no cytotoxicity for either cell line. the cytotoxic pro ...19921625320
detection of serum antibodies to bacillus piliformis in mice and rats using an enzyme-linked immunosorbent assay.rats and mice were infected with bacillus piliformis organisms at a dosage which resulted in clinical signs of tyzzer's disease in gerbils. although rats and mice did not show clinical signs of disease, rising antibody titers to b. piliformis were detected by enzyme-linked immunosorbent assay (elisa) 2 to 6 weeks post-inoculation and remained at positive levels 11 weeks post-inoculation. western blot analyses of sera from experimentally infected animals revealed banding patterns nearly identical ...19911849582
investigations into an antigen causing tyzzer's disease in mice (bacillus piliformis). 19807234164
an enzyme-linked immunosorbent assay for detection of anti-bacillus piliformis serum antibody in enzyme-linked immunosorbent assay (elisa) is described for the detection of rabbit serum antibody directed against the causative agent of tyzzer's disease, bacillus piliformis. ninety-four percent agreement was found between the elisa and an indirect fluorescent antibody test. the sensitivity of the elisa was 95% and its specificity was 92% as compared to the indirect fluorescent antibody test (ifat). the rabbit origin b. piliformis isolate used in this elisa was found to be cross-reactive by ...19873599886
correlation between megaloileitis and antibodies to bacillus piliformis in laboratory rat colonies.rat colonies in which antibodies to bacillus piliformis were detected in animals examined at the age of 8 to 15 weeks were compared with rat colonies where no such antibodies were present. the seropositive colonies had a low incidence of megaloileitis in 5-week-old rats of sprague-dawley stock and some few inbred strains. in seronegative colonies, no megaloileitis was detected. in rats with megaloileitis, significantly high titers to b. piliformis were noted and the agents could be identified in ...19921460842
rederivation of rat colonies seropositive for bacillus piliformis and the subsequent screening for antibodies.latent infection of rats in a breeding colony with bacillus piliformis detectable by antibodies to the agent in an immunofluorescence assay was eliminated by a combination of traditional rederivation techniques, using animal units not previously used for rat breeding, and the use of specific disinfection procedures. the success rate was apparently correlated with the use of peracetic acid instead of aldehyde products to decontaminate the animal unit.19921460841
mutual viral and bacterial infections after housing rats of various breeders within an experimental unit.fifteen athymic rat strains from 11 breeding colonies were housed within an experimental facility for an immunological study. health status records supplied with 14 of the strains listed infections by kilham's rat virus (krv), clostridium piliforme (bacillus piliformis) and pasteurella pneumotropica for 2, 2 and 1 colonies respectively. in sera taken previous to the study from euthymic rats of 10 strains, antibodies to krv were detected in 3 strains, to pneumonia virus of mice (pvm), rat corona ...19968709572
studies on the diagnosis of tyzzer's disease in laboratory rat colonies with antibodies against bacillus piliformis (clostridium piliforme).to develop a system to be used for confirming diagnosis of tyzzer's disease in seropositive rat colonies, 38 mol:sprd, bb/wor/mol-bb, and stroke-prone rats suffering from megaloileitis were examined. all affected rats had been found by a systematic examination of 5-week-old male rats from barrier-protected colonies, sero-positive to bacillus piliformis, the agent of tyzzer's disease. the rats were evaluated by serologic testing, histologic examination of the ileum, liver, and heart (hematoxylin ...19947531255
development of a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay for detection of bacillus piliformis isolate-specific antibodies in laboratory animals.a competitive inhibition enzyme-linked immunosorbent assay (elisa) was developed to detect bacillus piliformis isolate-specific antibodies in serum specimens from rats and gerbils experimentally infected with b. piliformis isolates r1, r2, or m. detection was based on the ability of serum antibodies to block binding of b. piliformis isolate-specific monoclonal antibodies to purified b. piliformis flagella. application of this assay to serum specimens collected from sham-infected or experimentall ...19947518016
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