| characterization of shiga toxin producing (stec) and enteropathogenic escherichia coli (epec) in raw yak (poephagus grunniens) milk and milk products. | thirty-one shiga toxin-producing (stec) and 6 enteropathogenic escherichia coli (epec) were isolated from 87 raw yak milk and 63 'churpi' samples. of 18 stx(1) positive isolates (48.6%), 14 carried stx(1c) (77.7%). subtyping of 28 stx(2) positive isolates (75.7%) revealed the presence of stx(2c) (9, 32.1%), stx(2d) (3, 10.7%), stx(2e) (1, 3.57%) and stx(2f) (3, 10.7%) variants. furthermore, intimin (eaea), enterohaemolysin (ehxa), autoagglutinating adhesin (saa), iha (adherence conferring protei ... | 2012 | 22226073 |
| potential antibacterial activity of berberine against multi drug resistant enterovirulent escherichia coli isolated from yaks (poephagus grunniens) with haemorrhagic diarrhoea. | to evaluate the antimicrobial efficacy of berberine, a plant alkaloid. | 2013 | 23608335 |
| prokaryotic expression and immunogenicity analysis of yak recombinant myostatin. | myostatin (mstn) is a negative regulator of skeletal muscle growth. the objective of the present study was to express yak (bos grunniens) recombinant mstn protein in e. coli and study its characteristics of immunogenicity. cdna encoding yak mstn mature peptide was amplified by reverse-transcription pcr, and cloned into pet28a(+) vector and expressed in e. coli. the expressed recombinant mstn was purified by affinity chromatography and used to prepare rabbit anti yak mstn antibody. the results sh ... | 2012 | 23134305 |
| prevalence of sulfonamide and florfenicol resistance genes in escherichia coli isolated from yaks (bos grunniens) and herdsmen in the tibetan pasture. | to determine the antimicrobial susceptibility profiles and prevalence of resistance genes in escherichia coli isolated from yaks (bos grunniens) and herdsmen in nine plateau pastures in tibet, we isolated 184 nonidentical strains of e. coli from yaks and herdsmen. antimicrobial susceptibility testing of 15 antimicrobials was conducted and the prevalence of sulfonamide resistance genes (sul1, sul2, and sul3) and florfenicol resistance genes (flor, cfr, cmla, fexa, pexa, and estdl136) was determin ... | 2015 | 25973625 |
| shiga toxin-producing escherichia coli in yaks (bos grunniens) from the qinghai-tibetan plateau, china. | shiga toxin (stx)-producing escherichia coli (stec) are recognized as important human pathogens of public health concern. many animals are the sources of stec. in this study we determined the occurrence and characteristics of the stec in yaks (bos grunniens) from the qinghai-tibetan plateau, china. a total of 728 yak fecal samples was collected from june to august, 2012 and was screened for the presence of the stx 1 and stx 2 genes by taqman real-time pcr after the sample was enriched in modifie ... | 2013 | 23776496 |
| beta-xylosidase activity of a gh3 glucosidase/xylosidase from yak rumen metagenome promotes the enzymatic degradation of hemicellulosic xylans. | aims: to characterize the duel activities of a glycosyl hydrolase family 3 β-glucosidase/xylosidase from rumen bacterial metagenome and to investigate the capabilities of its β-d-xylosidase activities for saccharification of hemicellulosic xylans. methods and results: a β-glucosidase/xylosidase gene rubgx1 was cloned from yak (bos grunniens) rumen using the metagenomic technology. recombinant rubgx1, expressed in escherichia coli, demonstrated high hydrolytic activities on both p-nitrophenyl-β ... | 2011 | 22085266 |
| molecular characterization and expression analysis of osteopontin cdna from lactating mammary gland in yak (bos grunniens). | osteopontin (opn) is a secreted phosphorylated glycoprotein. it has an important role in mammary gland development and lactation, as well as, is thought to be a potential candidate gene for lactation traits. in the present work, we isolated and characterized a full-length open reading frame (orf) of yak opn cdna from lactating mammary tissue, and examined its expression pattern in mammary gland during different stages of lactation, as well as, the recombinant opn protein of yak was expressed suc ... | 2011 | 21720759 |
| virulence gene and antibiotic resistance profile of shiga-toxin-producing escherichia coli prevalent in captive yaks (poephagus grunniens). | | 2009 | 19477084 |
| interferon-alpha genes from bos and bubalus bubalus. | interferon-a genes were cloned from six breeds of three species of two genera (three chinese native cattle breeds of yellow cattle, wild yak and huanhu domestic yak, one european breed of holstein cow, and two water buffalo breeds of fuan water buffalo and fuzhong water buffalo) by direct pcr. the pcr products were directly inserted into the expression vector to be sequenced and expressed. sequence analysis showed that ifn-a genes of six clones were composed of 498 nucleotides, encoding a mature ... | 2006 | 16621760 |