| cryptosporidium suis n. sp. (apicomplexa: cryptosporidiidae) in pigs (sus scrofa). | molecular and biological characteristics of a new species of cryptosporidium from the feces of pigs (sus scrofa) is described. oocysts are structurally indistinguishable from those of cryptosporidium parvum; they are passed fully sporulated, lack sporocysts, and measure 4.9-4.4 microm (mean = 4.6 microm) x 4.0-4.3 microm (mean = 4.2 microm); length to width ratio 1.1 (n = 50). cryptosporidium suis is not transmissible to nude mice and is poorly infectious for cattle. molecular and phylogenetic a ... | 2004 | 15357067 |
| prevalence and pathogenicity of cryptosporidium suis in pre- and post-weaned pigs. | a total of 4338 faecal samples, 135 of sows, 3368 of pre-weaned and 835 of post-weaned piglets from eight farms in south bohemia, czech republic were collected and examined for cryptosporidium infection. no sow, but 5.7% pre-weaned and 24.1% post-weaned piglets were positive for cryptosporidium infection. no relationship was found between diarrhoea and cryptosporidium infection in any of the different age groups (pre- and post-weaned piglets). four piglets, which were sporadically shedding crypt ... | 2006 | 16732883 |
| prevalence of cryptosporidium species in intensively farmed pigs in ireland. | natural cryptosporidium infections in pigs are widespread but generally apathogenic. this study was undertaken to determine the prevalence of zoonotic cryptosporidium spp. in piggeries in ireland, where the drinking water supply is particularly vulnerable to contamination with zoonotic species. overall, infections were detected in 39 out of 342 animals (11.4%), with highest infection rates among weaners (15%) and sows (13.3%). twenty-nine positive samples were genotyped based on ssu rrna sequenc ... | 2007 | 17565758 |
| molecular characterization of cryptosporidium isolates from pigs in zaragoza (northeastern spain). | a total of 142 stool specimens from pigs on 24 farms from the province of zaragoza (northeastern spain) were screened for cryptosporidium spp. samples were first analysed by routine techniques (formalin-ethyl acetate sedimentation method and modified ziehl-neelsen stain) selecting those microscopically positive for genetic characterization. cryptosporidium species and genotypes were determined by a nested pcr-rflp technique at the 18s ribosomal dna locus and sequencing of the pcr-positive second ... | 2007 | 17683866 |
| prevalence of cryptosporidium genotypes in pre and post-weaned pigs in australia. | a total of 289 pig faecal samples were collected from pre-weaned and post-weaned piglets and sows from 1 indoor and 3 outdoor piggeries located in the south-west region of western australia and screened at the 18s rrna locus for the presence of cryptosporidium. an overall prevalence of 22.1% (64/289) was identified. cryptosporidium was more prevalent in post-weaned animals (p<0.05); 32.7% (51/156) versus 10.6% (13/123) for pre-weaned animals. sequence analysis identified cryptosporidium suis in ... | 2008 | 18486131 |
| molecular characterization of cryptosporidium isolates from pigs at slaughterhouses in south bohemia, czech republic. | a total of 123 fecal samples of slaughtered finisher pigs and 21 sows from 14 farms were screened for cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method. positive samples were molecularly characterized by direct sequencing of partial small subunit ribosomal rna (ssu rrna) and gp60 partial genes and polymerase chain reaction restriction fragment length polymorphism of ssu rrna. cryptosporidium oocysts were microscopically identified in 36 finishers (29%) and two ... | 2009 | 18850112 |
| prevalence and age-related infection of cryptosporidium suis, c. muris and cryptosporidium pig genotype ii in pigs on a farm complex in the czech republic. | a total of 413 pig faecal samples were collected from pre-weaners (119), starters (131), pre-growers (123) and sows (40) from a farm with a closed breeding system segmented into two breeding complexes and a growing complex in the region of vysocina, czech republic and screened for the presence of cryptosporidium using staining methods and genotyping (ssu rrna). cryptosporidium oocysts were detected by microscopy in the faeces of 21.1% of the samples (87/413). sequence analyses and rflp identifie ... | 2009 | 19091471 |
| concentrations, viability, and distribution of cryptosporidium genotypes in lagoons of swine facilities in the southern piedmont and in coastal plain watersheds of georgia. | waste lagoons of swine operations are a source of cryptosporidium oocysts. few studies, however, have reported on oocyst concentrations in swine waste lagoons; none have reported on oocyst viability status, nor has there been a systematic assessment of species/genotype distributions across different types of swine facilities. ten swine waste lagoons associated with farrowing, nursery, finishing, and gestation operations were each sampled once a month for a year. oocysts were extracted from tripl ... | 2010 | 20639363 |
| prevalence and molecular identification of cryptosporidium spp. in pigs in henan, china. | the distribution and public health significance of cryptosporidium species/genotypes in pigs differ among geographic areas and studies. to characterize the prevalence of cryptosporidiosis in pigs in henan, china, a total of 1,350 fecal samples from 14 farms in ten prefectures in henan province were examined. the overall prevalence of cryptosporidium was 8.2% (111/1,350), with the highest infection rate (79/383 or 20.6%) in 1-2-month-old piglets and the lowest infection rates in 3-6-month-old pig ... | 2010 | 20697908 |
| new view on the age-specificity of pig cryptosporidium by species-specific primers for distinguishing cryptosporidium suis and cryptosporidium pig genotype ii. | two species of cryptosporidium are commonly identified in pigs: cryptosporidium suis and cryptosporidium pig genotype ii. detection of cryptosporidium spp. is routinely based on molecular methods such as polymerase chain reaction (pcr) and subsequent restriction fragment length polymorphism (rflp) or gene sequencing. however, most of these methods are hampered by low sensitivity to mixed infections. as a solution of this problem, novel species-specific primers were designed and tested in the pre ... | 2010 | 21131129 |
| prevalence of cryptosporidium spp. in pigs in shanghai, china. | a survey on prevalence of cryptosporidium spp. in pigs at 12 farms in 8 suburban districts and 1 county of shanghai was conducted under sheather's sucrose flotation protocol and modified acid-fast stain methods from 2006 to 2009. a total of 2323 faecal samples were collected and cryptosporidium spp. oocysts were detected in 800 samples (34.4%). cryptosporidium was found in all 12 pig farms. significant variations of prevalence were observed in different farms ranging from 14.1% to 90.6%. a follo ... | 2011 | 21616599 |
| occurrences and genotypes of cryptosporidium oocysts in river network of southern-eastern china. | transportation of cryptosporidium oocysts in river type source water is of great concern in an area where extensive human activities exist. in this study, a total of 47 samples were collected from tongxiang, china, where drinking source water was taken from a complicated river network system, by three sampling campaigns over a rainy season in 2009, to reveal the presence, genotypes, and likely source of cryptosporidium oocysts in river water. immunofluorescence microscopy analyses show that 37 ( ... | 2011 | 22006191 |
| the first report on cryptosporidium suis and cryptosporidium pig genotype ii in eurasian wild boars (sus scrofa) (czech republic). | a total of 193 faecal samples of adult eurasian wild boars were collected at 12 enclosures across the czech republic and examined for cryptosporidium infection using both microscopic and molecular tools. cryptosporidium oocysts were not detected in any of the 193 faecal samples examined using the aniline-carbol-methyl violet staining method. thirty-two positive cases of cryptosporidium infection were detected using either genus- or species-specific nested pcr. mono-infection with cryptosporidium ... | 2011 | 21917378 |
| cryptosporidium suis and cryptosporidium scrofarum in eurasian wild boars (sus scrofa) in central europe. | from 2011 to 2012, to identify cryptosporidium spp. occurrence in eurasian wild boars (sus scrofa) 29 randomly selected localities (both forest areas and enclosures) across the central european countries of austria, the czech republic, poland, and the slovak republic were investigated. cryptosporidium oocysts were microscopicaly detected in 11 out of 460 faecal samples examined using aniline-carbol-methyl violet staining. sixty-one cryptosporidium infections, including the 11 infections that wer ... | 2013 | 23916060 |
| presence of cryptosporidium scrofarum, c. suis and c. parvum subtypes iiaa16g2r1 and iiaa13g1r1 in eurasian wild boars (sus scrofa). | the aim of the present study was to identify the species of cryptosporidium infecting eurasian wild boars (sus scrofa) in galicia (nw, spain). a sampling of 209 wild boars shot in different game preserves was carried out during the hunting season in 2009-2010. all samples were examined for cryptosporidium infection, using both immunological and molecular tools. cryptosporidium oocysts in faecal samples were identified using a direct immunofluorescence technique with monoclonal antibodies (dfa). ... | 2013 | 23643454 |