| development and characterization of monoclonal antibodies to first-generation merozoites of eimeria bovis. | merozoites of eimeria bovis were harvested from bovine monocyte cell cultures and used to immunize balb/c mice. spleens from immunized mice were removed and the cells fused with mouse myeloma cells. supernates from resulting hybridoma cell lines were examined for antibodies to first-generation e. bovis merozoites using an indirect immunofluorescent antibody (ifa) assay. three positive cell lines were identified and cloned by limiting dilution. all three cell lines produced immunoglobulins of the ... | 1992 | 1281590 |
| demonstration of isospora suis oocysts in faecal samples. | | 1992 | 1455595 |
| [beta-d-glucosidase in the microvillous zone of small intestine enterocytes in experimental coccidiosis in suckling piglets]. | in the small intestine mucosa of 24 gnotobiotic farrows experimentally infected with the oocysts of coccidiosis of isospora suis (infection administration--100,000 oocysts) on the first day after the delivery, we carried out the microdensitometric evaluation of the activity of beta-d-glucosidase (phlorizin-hydrolase; hetero-beta-galactosidase; lactase-beta-glucosidase complex; ec. 3.2.1.21). great attention was paid to the topochemistry of enzyme, deposited in a microvillous zone of enterocytes. ... | 1991 | 1771725 |
| pathogenesis of experimental combined infections with isospora suis and rotavirus in conventional and gnotobiotic piglets. | 51 gnotobiotic and 63 conventional, one-, or two-days-old piglets were divided into five groups and infected orally either with isospora suis or rotavirus alone, or with both agents simultaneously or successively with alternative sequences and various intervals. 15 gnotobiotic and 10 conventional piglets served as controls. the development of small intestinal lesions after infection with i. suis was biphasic. the dominant alteration resulting from rotavirus infection was villus atrophy, consider ... | 1991 | 1858460 |
| experimental isospora suis infections in miniature swine. | clinical responses to experimental isospora suis infections were compared in sinclair miniature pigs and cross-bred conventional pigs. pre-patent periods, fecal consistencies, oocyst excretion dynamics, trends in surviving pig weights and lesions were similar in infected miniature and conventional pigs. the results indicate that the susceptibility of miniature pigs to i. suis is similar to that of conventional pigs. these findings should encourage their use as models for the study of neonatal co ... | 1991 | 1882501 |
| [lysosomal activity in enterocytes in the small intestine in piglets experimentally infected with isospora suis]. | the lysosomal activity of enterocytes of the small intestine mucosa was investigated in gnotobiotic and conventional piglets experimentally infected on the first day after birth (dab) by the oocysts of the coccidia isospora suis. a method of the proof of beta-d-glucuronidase (ec.3.2.1.31.) activity was used to demonstrate lysosomes. the piglets were infected by different infection doses of oocysts (100,000 oocysts in gnotobiotic piglets and 200,000 oocysts in conventional piglets). in the gnotob ... | 1991 | 1926681 |
| [density of selected enzymes in the goblet cells of the small intestine in piglets experimentally infected with the coccidium isospora suis]. | the density of selected enzymes in the goblet cells of the mucous membrane of the small intestine was studied in a group of 12 gnotobiotic piglets experimentally infected with the coccidium isospora suis one day after parturition (dpp), using the vickers m-786 scanning and integrating microdensity meter. at an infecting dose of 100,000 oocysts of i. suis, the histochemistry of the goblet cells of the mucous membrane of the piglets changed significantly in the period of 4 to 10 days after infecti ... | 1990 | 1978431 |
| [densitometric analysis of aminopeptidase m activity in small intestine mucosa in piglets experimentally infected with isospora suis]. | in gnotobiotical and conventional piglets infected a day post partum (dpp) with oocysts of the coccidium isospora suis, densitometrical analysis of the activity of aminopeptidase m (ec.3.4.11.2; apm) was performed in the area of microvillous zone of the small intestine. piglets were infected with different infection doses of oocysts (100,000 oocysts) in gnotobiotical piglets and 200,000 oocysts in conventional piglets). in infected gnotobiotical piglets, the apm activity was studied in the perio ... | 1990 | 1982978 |
| [histochemistry of selected peptidases in small intestine mucosa in piglets experimentally infected with isospora suis]. | in the small intestine mucosa of 24 gnotobiotical piglets experimentally infected the first day post partum with oocysts of the coccidium isospora suis, the activities of dipeptidylpeptidase iv (ec.3.4.14.5.; dap iv) and gamma-glutamyl transferase (ec.2.3.2.2.; ggt) in the microvillous zone of enterocytes were evaluated by scanning densitometry. the tissue of the small intestine in piglets infected with a dose of 100,000 oocysts of the coccidia of i. suis was examined in the period from the firs ... | 1990 | 1983128 |
| ultrastructure of developing isospora suis in cultured cells. | the ultrastructure of isospora suis sporozoites, type-1 meronts, and type-1 merozoites was examined, using transmission electron microscopy of infected cultured cells. the ultrastructure of sporozoites and type-1 merozoites was similar. each possessed trimembranous pellicles, subpellicular microtubules, a conoid, anterior and posterior polar rings, rhoptries, micronemes, a single vesicular nucleus, tubular mitochondria, golgi complexes, ribosomes endoplasmic reticula, inactive micropores, amylop ... | 1991 | 2035924 |
| [alkaline phosphatase activity in goblet cells in the small intestine of piglets experimentally infected with the coccidium isospora suis]. | alkaline phosphatase activity (ec. 3.1.3.1.) in goblet cells was investigated in the small intestine of 16 gnotobiotic piglets infected one day after delivery (dad) by different rates of oocysts of isospora suis coccidia. at a high infection rate of i. suis (750,000) the goblet cells were found to be highly positive to alkaline phosphatase on day 3 to day 4 after infection (dai). in piglets infected by a low infection rate of i. suis oocysts (100,000) the activity of alkaline phosphatase activit ... | 1990 | 2219627 |
| double alteration of the small intestine in conventional and gnotobiotic piglets experimentally infected with the coccidium isospora suis (apicomplexa, eimeriidae). | the pathogenicity of the coccidium isospora suis was studied in 42 conventional and 26 gnotobiotic piglets at 1-13 days post infection (dpi). the prepatent period of the coccidium i. suis was 4.5-5 days. only in one experiment in conventional piglets the prepatent period of 3.5 days was recorded. at least 9 days pathogenesis of i. suis was recorded during which double alteration of the small intestine occurred. the first alteration was found at 3-4 dpi. the second phase of alterative changes was ... | 1990 | 2332196 |
| [activity of mucosubstances and the goblet cell count in the large intestine of piglets infected with the coccidium, isospora suis]. | in a group of conventional and gnotobiotic piglets experimentally infected with the isospora suis coccidia the quantitative presence of acid and neutral mucous substances in the large intestine and the counts of goblet cells in the surface mucosa and in lieberkühnis crypts (in the following text called just the crypts) were investigated. in conventional piglets infected with the dose of 200,000 oocysts of i. suis coccidia the lowest content of acid mucous substances was recorded from the eighth ... | 1990 | 2333678 |
| [morphometric analysis of the activity of alkaline phosphatase in the small intestine of piglets infected with the coccidium isospora suis]. | gnotobiotical one-day-old piglets were infected with 100,000 isospora suis coccidia oocysts, and were immediately killed. in piglets killed on the 3rd to 11th day after infection (dai), the morphometric analysis of alkaline phosphatase activity was performed in the area of the microvillus zone of small intestine. in the control 2-7 days old animals, the small intestine was not equally supplied with alkaline phosphatase. in duodenum the activity reached 88.37 per cent of the active length of abso ... | 1990 | 2375068 |
| [the ratio of enterocytes and goblet cells in the mucosa of the small intestine in experimental infection of piglets with the coccidium isospora suis]. | the proportions of cup-shaped cells and enterocytes were studied in piglets infected the first and fifth day after birth with 200,000 oocysts of isospora suis. the greatest imbalance was found in the ratio of the cells of the intestinal mucous membrane in the region of middle and lower jejunum (ratio of 10:1.75)--this was recorded the third to fourth day after infection when the ratio made 10:0.62, whereas in the control animals it was 10:5.7. on the eighth to ninth day after infection the numbe ... | 1989 | 2494785 |
| [alkaline phosphatase activity levels during infection with the coccidia isospora suis in piglets]. | the activity of alkaline phosphatase (ec 3.1.3.1.) was studied in the small intestines of piglets infected with the coccidia isospora suis on the first and fifth day of age. alkaline phosphatase activity is reduced significantly the third to fourth day after infection (dai) throughout the small intestine in piglets infected on the fifth day of age. similar results were obtained in the piglets killed the eighth to tenth dai which had also been infected on the fifth day of their age. piglets infec ... | 1989 | 2728264 |
| [activity of selected dehydrogenases and monoamine oxidases in the small intestine of gnotobiotic piglets infected with the coccidium isospora suis]. | in the small intestine of 16 gnotobiotic piglets infected a day post partum (dpp) by isospora suis coccidia the activities were studied of selected dehydrogenases and monoaminoxidase (o2 oxidoreductase, maox, ec 1.4.3.4.). the following dehydrogenases were investigated: succinate dehydrogenase (sdh, ec 1.3.99.1.), glycerol-3-phosphate dehydrogenase (glycerol-3-phosphate:menadion oxidoreductase, gpox, ec 1.1.99.5.) and tetrazolium oxidoreductase (nadh, es 1.6.99.3.). the activities of nadh and gp ... | 1989 | 2756618 |
| [the observation of coccidia in swine]. | the coccidia species of domestic pig and wild boar were compared and defined morphologically and the sporogony of both was examined. no difference could be detected between the coccidia species of domestic pig and wild boar. six eimeria and one isospora species were found: eimeria debliecki, e. polita, e. porci, e. scabra, e. spinosa, e. suis and isospora suis. e porci could be found in austria for the first time. e. cerdonis vetterling, 1965 is a synonym of e. polita pellérdy, 1949. | 1989 | 2782664 |
| [coccidiosis in suckling calves raised on large farms]. | in a large herd of pigs whose piglets suffered from diarrheas coprological examination revealed the great incidence of isospora suis coccidia in piglets. the first oocysts were found out in seven-day piglets; the highest incidence was observed in twelve-day piglets (65.9%); the time of oocyst elimination (patency) was five to six days. the oocysts appeared in the excrements of piglets two to three days after the diarrhea onset. individual examinations of piglets demonstrated the correlation betw ... | 1986 | 3101268 |
| serum proteins of neonatal pigs orally inoculated with isospora suis oocysts. | serum proteins were evaluated by agarose electrophoresis at periodic intervals between postinoculation days (pid) 2 and 35 in 4 litters of pigs inoculated orally at 36 to 38 hours of age with 70,000 sporulated isospora suis oocysts and in 4 litters of age-matched noninoculated control pigs. in inoculated pigs, clinical disease characterized by vomiting and diarrhea began at pid 3 to 4 and was resolved by pid 11. isospora suis oocysts were detected in feces of inoculated pigs from pid 5 to 25, wi ... | 1988 | 3358549 |
| [mucus synthesis in the goblet cells of the small intestine in experimental infection with the coccidium isospora suis in piglets]. | the state of mucus synthesis in the goblet cells of the small intestine was studied in conventional piglets infected with a dose of 200,000 oocytes of the coccidium isospora suis the first and fifth day after parturition. the synthesis of mucus and its chemical characteristics undergo significant changes during the third and fourth day after infection. the activity of acid and neutral mucous substances declines; their level and the physiological synthetic function of goblet cells begin to return ... | 1989 | 2631376 |
| [use of the anticoccidial agent, tortrazuril (baycox, bayer) in coccidiosis in suckling pigs]. | at present neonatal coccidiosis caused by isospora suis is known to be an important disease in piglets. the coccidium i. suis was diagnosed as the causative agent of diarrhoeic disease in piglets on a large pig farm with continuous farrowing operation. the diagnosis of coccidiosis was based on clinical history, gross lesions, histopathology, stained impression smears and aided by detection of oocysts in the faeces. coccidiosis was associated with diarrhoea in piglets at the age of five to fourte ... | 1991 | 1841476 |
| [histochemistry of acid phosphatase in small intestine mucosa in experimental coccidiosis in suckling piglets]. | the activity of acid phosphatase (phosphohydrolase of orthophosphate monoesters; ec. 3.1.3.2) was evaluated densitometrically in the mucosa of duodenum, jejunum and ileum of 22 conventional piglets which were experimentally infected by oocysts of the coccidiae isospora suis (infection dose of 200,000 oocysts) on day one after parturition (dap). the activity of the studied hydrolase was investigated in the infected piglets during days two to ten after infection (dai) in the intestinal mucosa (ent ... | 1991 | 1807026 |
| development of isospora suis from pigs in primary porcine and bovine cell cultures. | sporozoites of isospora suis penetrated and developed by endodyogeny in primary porcine kidney (ppk) and primary fetal bovine kidney (pfbk) cell cultures. motile merozoites and binucleate type i meronts were observed in both types of cultured cells. multinucleate type ii meronts developed in ppk cell cultures only. these multinucleate meronts were always found singly, were nonmotile and did not form merozoites. | 1987 | 3617433 |
| isospora suis in pigs with post weaning diarrhoea. | | 1988 | 3381428 |
| parasite transmission in confined hogs. | examinations of fecal specimens from swine of all ages maintained in a totally enclosed confinement facility in south georgia were conducted in a 5-year survey (1977-1981) to determine the prevalence, intensity, and transmission patterns of intestinal nematode and protozoan parasites. weaned pigs in the nursery had no detectable parasitic infections except sporadic isospora suis in newly weaned pigs. growing-finishing hogs had a low prevalence of ascaris suum, oesophagostomum spp. and balantidiu ... | 1986 | 3705423 |
| porcine coccidia in papua new guinea. | faecal samples from 232 domestic pigs raised on concrete, 98 free-ranging village pigs, and five wild boar showed 46.6 (108/232), 54 (53/98) and 80% (4/5) prevalence of coccidian oocysts, respectively. eight species of eimeria, and isospora suis, were recovered. in their descending order of predominance in the pigs raised on concrete, the species of coccidia were e. debliecki (26.7%), e. scabra (22.4%), e. neodebliecki (19.8%), e. porci (15.5%), e. suis (11.6%), e. polita (8.6%), e. perminuta (7 ... | 1986 | 3727342 |
| interfering effect of isospora suis infection on salmonella typhimurium infection in swine. | piglets were each orally inoculated daily for 4 consecutive days, with 10(4)-10(5) nalidixic acid-resistant salmonella typhimurium and, starting 1 day after inoculation, with 50 000 isospora suis sporulated oocysts. control pigs were given s. typhimurium alone following the same inoculation schedule. fecal samples were taken 5, 7 and 10 days after the coccidial inoculation, and all the pigs were necropsied 13 days after inoculation. s. typhimurium counts in the feces and in the cecal contents an ... | 1985 | 3890353 |
| pathogenicity of isospora suis in gnotobiotic and conventionalised piglets. | isospora suis is unequivocally a primary pathogen of swine. inoculation of i suis in conventionalised and germ-free piglets caused a biphasic disease course with marked diarrhoea, villous atrophy and necrosis of the intestinal epithelium at four to six and eight to 10 days after inoculation. the presence of a normal bacterial flora markedly (p less than 0.05) influenced the survival rate of piglets but did not appear to affect the histopathological changes observed. mild limited focal necrosis a ... | 1985 | 4024419 |
| effects of experimentally induced isospora suis infection on morbidity, mortality, and weight gains in nursing pigs. | forty-nine pigs from 6 litters were inoculated at 3 days of age with 300,000 sporulated oocysts of isospora suis to determine the effects of neonatal coccidiosis on morbidity, mortality, and weight gain in nursing pigs. fifty-one control pigs from 6 litters were not inoculated. three to 5 days after inoculation, the inoculated pigs developed a nonhemorrhagic diarrhea, generally lasting 6 to 10 days, that lead to visible dehydration, loss of condition, and 20.4% mortality. control pigs did not di ... | 1985 | 4026033 |
| experimental coccidiosis (isospora suis) in a litter of feral piglets. | | 1985 | 4032631 |
| isospora suis species in a danish spf-herd. | in two studies carried out in 1974-75 on 10 eight and 25 two week old piglets obtained from a spf-herd the endoparasitic status was determined. samples of faeces were examined by microscopy which revealed the presence of isospora suis, a finding that was quite unexpected in a spf-herd. during days 17-23 pp. the 25 piglets were not found to excrete oocysts, the earliest findings of oocysts being made 24-35 days p.p. the 10 piglets being kept isolated in individual pens excreted oocysts up until 8 ... | 1985 | 4034389 |
| the sporulation time of isospora suis oocysts from different sources. | feces containing isospora suis oocysts were collected from naturally- and experimentally-infected pigs from four different areas of the united states. the unsporulated oocysts were cleaned, concentrated, mixed with 2.5% aqueous potassium dichromate solution, poured into petri dishes to a depth of 5 mm, and incubated at 25 degrees c. the oocysts were examined with a microscope at 12 h intervals and the stages of sporulation present were counted. although a few oocysts were completely sporulated a ... | 1986 | 3788016 |
| experimental transmission of intestinal coccidiosis to piglets: clinical, parasitological and pathological findings. | twenty-eight piglets coming from a "specific pathogen free" herd were inoculated at three days of age with 50 000 or 100 000 sporulated oocysts of isospora suis. fecal samples were examined for oocyst shedding daily and several clinical parameters were recorded. ten piglets were used as normal controls. groups of piglets were euthanized from three days to 12 days postinoculation and routine necropsies were performed. bacteriological, virological, parasitological and histopathological examination ... | 1983 | 6230142 |
| field study of coccidial and rotaviral diarrhoea in unweaned piglets. | a study of diarrhoea in unweaned piglets was carried out in nine herds, with special reference to the enteropathogenic agents which could be demonstrated. coccidial (isospora suis) and rotaviral infections were both identified, either singly or in combination. more extensive studies of i suis infection were undertaken in two of the herds and it was found that diarrhoea occurred most commonly in five- to 14-day-old piglets. piglets with i suis infection were not necessarily diarrhoeic but grew po ... | 1982 | 6278696 |
| reduced pre-patent period in experimental infection of piglets with the coccidium isospora suis. | | 1987 | 3583128 |
| pathology of natural isosporosis in nursing piglets. | in piglets suffering from natural coccidiosis, post-mortem examination showed that pathological changes induced by isospora suis were evident from day 7 to day 14 of life, and particularly, by days 9 and 10. macroscopically, the changes were manifest as enteritis varying from catarrhal to pseudomembraneous form. microscopically, they consisted of more or less extensive atrophy of villi whose apical parts were necrotic, of metaplasia and erosion of epithelium. with the exception of duodenum and t ... | 1987 | 3666610 |
| isospora suis infection in piglets. a review. | recently several reports have been published on the association of isospora suis with diarrhea in piglets. a review is given of i. suis infection in piglets, in which the taxonomy, life cycle, pathogenicity, immunity, and treatment of i. suis and related organisms are discussed. | 1983 | 6359663 |
| control of isospora suis-induced coccidiosis on a swine farm. | results of a program designed to control neonatal porcine coccidiosis on a total confinement, farrow-to-finish swine farm are reported. the control program consisted of washing, phenol disinfection, and steam cleaning of farrowing houses and treatment of sows with amprolium hcl before and after farrowing. before initiation of the control program, 88.9% of the sows examined in the farrowing house were negative for coccidian oocysts, 9.9% were positive for eimeria spp, and 1.2% were positive for i ... | 1985 | 4039545 |
| life cycle of isospora suis in gnotobiotic and conventionalized piglets. | isospora suis had 3 asexual and 1 sexual intra-intestinal conventional life cycle. the first asexual generation was most prominent at 2 days p.i. (post inoculation) and produced 2-7 merozoites. the second-generation meronts were prevalent at 3-4 days p.i. and produced 2-12 large merozoites. at 4-5 days p.i. the third generation meronts were prominent and produced 4-24 small crescent shaped merozoites. mature sexual stages were most prominent at 5-6 days p.i. the stages were most numerous in the ... | 1984 | 6543059 |
| neonatal diarrhea of pigs in quebec: infectious causes of significant outbreaks. | to evaluate the relative importance of the various enteropathogens causing neonatal diarrhea in quebec farrowing operations, observations were made on 749 diarrheic pigs from 325 outbreaks of diarrhea. they were one to 15 days of age, and were obtained alive for necropsy generally within 48 hours of the onset of diarrhea. some pigs were from severe, explosive outbreaks of diarrhea with high morbidity and mortality rates, while others were from herds with chronic neonatal diarrhea with lower morb ... | 1983 | 6299483 |
| motility of isospora suis meronts. | | 1983 | 6631645 |
| epidemiology of porcine neonatal steatorrhoea in sweden. 1. prevalence and clinical significance of coccidal and rotaviral infections. | a study of neonatal steatorrhoea in unweaned piglets was carried out in randomly visited herds. isospora suis and rotavirus were shown to be incriminated in this type of enteric disease and these agents may be considered important enteropathogens for 1-3-week-old piglets in sweden. more extensive studies of i. suis were undertaken in one large herd. it was found that steatorrhoea may appear in 4-day-old piglets but oocysts were not detected until some days latter. piglets that were shedding i. s ... | 1984 | 6330668 |
| complete development of isopora suis of swine in chicken embryos. | development of the swine coccidium , isospora suis, in embryonated chicken eggs is described. the allantoic cavities of eight-to-ten-day-old white leghorn embryos were inoculated with either 100,000 or 200,000 sporozoites. developmental stages morphologically similar to those found in the intestines of piglets were present in the endodermal layer of the chorioallantoic membrane (cam), beginning three days post inoculation (pi). no stages were found in the mesodermal or ectodermal layers of the c ... | 1984 | 6737316 |
| [cryptosporidium sp. and isospora suis in swine in italy]. | the presence of cryptosporidium sp. and isospora suis is reported for the first time in piglets in italy, in the province of mantova: the first one has been found in 1 and the second one in 39 out of 200 litters. two piglets, respectively 20-day-old and 24-day-old, were experimentally infected with cryptosporidium sp. oocysts from naturally infected calves, without clinical signs. | 1984 | 6600003 |
| coccidiosis in swine: effect of disinfectants on in vitro sporulation of isospora suis oocysts. | | 1981 | 6914860 |
| coccidiosis in swine: a search for extraintestinal stages of isospora suis. | clinical coccidiosis in swine is associated with isospora suis infection of piglets. the endogenous life cycle of i suis in piglets occurs within the superficial epithelium of the small intestine. extraintestinal stages of i suis were not demonstrated in tissues of experimentally infected piglets or mice. feeding these tissues to previously uninfected piglets did not result in oocyst shedding of consequence. extraintestinal stages do not seem to occur in piglets with i suis as in some other isos ... | 1982 | 7064330 |
| prevalence of oocysts of isospora suis and eimeria spp from sows on farms with and without a history of neonatal coccidiosis. | oocysts of isospora suis were not found in any of 77 fecal samples from sows on farms with a history of neonatal coccidiosis. oocysts of isospora suis were found in 1 of 172 fecal samples from farms without a history of neonatal coccidiosis. oocysts of eimeria spp were found in 81.8% of the sows from farms with a history of neonatal coccidiosis and in 94.8% of the sows from farms without a history of neonatal coccidiosis. oocysts of isospora spp from birds were encountered as a pseudoparasite in ... | 1984 | 6469839 |
| coccidiosis in swine: dose and age response to isospora suis. | coccidiosis is a disease of the young piglet due to infection with isospora suis and is characterized by diarrhea which is nonresponsive to antibacterial therapy. there is variable morbidity and mortality. piglets develop a more severe clinical illness and enteritis when infected with i. suis at one to three days of age than when infected at two weeks of age. microscopic lesions range from villous atrophy and mild erosion to severe fibrinonecrotic enteritis. | 1982 | 6889908 |
| excystation of isospora suis biester, 1934 of swine. | the in vitro excystation of sporozoites of isospora suis biester 1934 is described. sporocysts of i. suis lack a stieda body. upon incubation in 0.75% sodium taurocholate or in 0.25% trypsin + 0.75% sodium taurocholate excystation solutions, sporozoites were released by separation of the sporocyst wall into four plates. occasionally, the sporocyst wall did not separate completely but opened partially and released the sporozoite. at the time of excystation, sporozoites were short and broad but be ... | 1983 | 6837100 |
| demonstration of immunity against isospora suis in swine. | piglets naturally exposed or experimentally infected with isospora suis oocysts were given challenge doses of oocysts to determine the extent of development of immune resistance. piglets in both studies shed low numbers of, or no detectable oocysts, following challenge. administration of methylprednisolone acetate failed to induce oocyst shedding in previously infected piglets. piglets rechallenged with i. suis following steroid injections also failed to shed significant numbers of oocysts sugge ... | 1982 | 7201193 |
| ultrastructural evidence of endodyogeny in isospora suis from pigs. | | 1982 | 7072320 |
| sporogony of isospora suis biester, 1934 of swine. | sporogony of isospora suis of swine is described. unsporulated oocysts were collected immediately after defecation, mixed in 2.5% potassium dichromate solution, incubated at various temperatures, examined at different time intervals, and classified as to stage of development. the uninucleate sporont underwent nuclear division and became binucleated, and then divided to form two round, uninucleate sporoblasts. binucleate sporoblasts elongated to form binucleate sporocysts, each of which then gave ... | 1982 | 7131190 |
| coccidia of feral swine from florida. | fecal samples from 251 free-ranging feral swine (sus scrofa) in florida were examined for coccidia. ten species of coccidia were recovered. in descending order with respect to prevalence, they were eimeria porci, e debliecki, e neodebliecki, e scarbra, e suis, e cerdonis, e polita, e perminuta, isospora suis, and e spinosa. of the swine examined, 87% were passing oocysts and 82% of the infected individuals were shedding at least 2 species of coccidia. all age classes included infected individual ... | 1982 | 7174441 |
| the use of toltrazuril for the prevention of coccidiosis in piglets before weaning. | to determine the efficacy of toltrazuril as a prophylactic treatment for coccidiosis in piglets caused by isospora suis (i suis), a single 1.0 ml dose of toltrazuril was administered orally to 1056 piglets between 3 and 6 days of age, in 5 piggeries. prophylactic treatment of piglets reduced the occurrence of coccidiosis in litters from 71% to 22%. the number of antibacterial treatments given and the number of piglets affected per litter were also significantly reduced, resulting in some improve ... | 1995 | 7646378 |
| [the development of weaned piglets experimentally infected with isospora suis as a model for the effect of lasalocid and halofuginone on coccidia (author's transl)]. | piglets which were early-weaned at the age of 21.7 days and experimentally monoinfected with oocysts of isospora suis showed distinct reductions in zootechnical criteria during an experimental period of 4 weeks. the daily liveweight gains in the infected piglets (group b) was 19.7% lower than in the control group a, which was free of coccidia. comparative photographs with the rem showed serious lesions in the small intestine of infected piglets, which are thought to be mainly responsible for the ... | 1981 | 7223136 |
| shedding of oocysts in piglets experimentally infected with isospora suis. | forty-seven piglets were inoculated with doses of 100 to 50,000 sporulated oocysts of isospora suis. after 5-7 days oocysts were found in faeces. the patent period extended from 8 to 16 days. the shedding of oocysts showed a cyclic pattern with 2-3 peaks separated by intervals of approximately 5 days. subpatent periods were often seen between the peaks. the level of oocyst shedding during the initial days of the patent period reflected, to some extent, the inoculation dose. however, a maximum of ... | 1994 | 7942382 |
| studies on preweaning piglet diarrhoea. | the aim of this study was to determine the cause and risk factors involved in preweaning piglet diarrhoea. faecal samples from 2380 diarrhoeic piglets, 5 to 30 days of age, were examined for enteropathogens. isospora suis oocysts were detected in 53.8% of samples, escherichia coli in 18.2% and rotavirus in 16.9%. i suis had the widest distribution, being present on 70.9% of 151 piggeries. the onset of diarrhoea occurred mainly between 7 and 14 days (77.5%), peaking at 10 days. records of 4086 li ... | 1993 | 8368968 |
| [the epizootiology and pathogenic significance of infections with isospora suis in ten piglet production operations in nordrhein-westfalen]. | from july 1993 to june 1994 2036 fecal samples from 196 litters from 10 pig farms were collected. three or four piglets from each litter and the respective sows were examined 3 times at 2, 3 and 4 weeks after farrowing. i. suis was detected in all 10 farms. the rate of infection of the litters was 62.2%. 71.6% of all litters with diarrheic problems were i. suis positive. the percentage of i. suis in the feces of piglets was 17.4% at the age of 2 weeks, 41.3% at the age of 3 weeks and 39.1% at th ... | 1996 | 8765537 |
| [pathomorphological changes in the small intestine of pigs with rotavirus diarrhea]. | studied were the pathohistological changes and the morphologic-structural injuries of intestinal mucosa of pigs (5-7 days old), spontaneously affected with rotavirus diarrhea, raised by traditional methods. simultaneously with the rotavirus invasion was established isospora suis. in spite of the early time of the disorder (the test was carried out 24 hours after the appearance of the first clinical symptoms) there were established different in degree and expression morphologic changes; atrophy a ... | 1987 | 3445448 |
| prevalence of porcine neonatal isosporosis in brazil. | the prevalence of isospora suis and clinical signs of isosporosis were observed in 33 swine farms from 20 sites in the southeastern state of são paulo, brazil. the study was performed by collecting 177 faecal samples from nursing and weaned piglets. a history of clinical neonatal isosporosis, as well as the type of farrowing and nursery houses and the pig management in the farms were correlated to the prevalence of i. suis oocysts. six faecal samples were collected in each of the farms (two from ... | 1996 | 9017865 |
| the time course of the specific antibody response by various elisas in pigs experimentally infected with toxoplasma gondii. | with the aim of developing routine serological tests for monitoring the toxoplasma infection status of danish swine herds, four elisas based on tachyzoite antigen were set up: (1) an indirect elisa for igg-antibody; (2) a blocking elisa for antibody to the membrane antigen, p30; (3) an indirect elisa for igm; (4) a reverse, antibody-catching igm-elisa. groups of pigs (number between 6 and 10) were inoculated with tachyzoites of the rh-strain, tissue cysts of two complete strains, or oocysts in t ... | 1997 | 9231984 |
| phylogenetic analysis of coccidia based on 18s rdna sequence comparison indicates that isospora is most closely related to toxoplasma and neospora. | the phylogenetic relationships and taxonomic affinities of coccidia with isosporan-type oocysts have been unclear as overlapping characters, recently discovered life cycle features, and even recently discovered taxa, continue to be incorporated into biological classifications of the group. we determined the full or partial 18s ribosomal rna gene sequences of three mammalian isospora spp., isospora felis, isospora ohioensis and isospora suis, and a sarcocystis sp. of a rattlesnake, and used these ... | 1998 | 9561772 |
| complete development of the porcine coccidium isospora suis biester, 1934 in cell cultures. | development from inoculated sporozoites to unsporulated oocysts of isospora suis biester, 1934 is described in a swine testicular (st) cell line. sporozoites penetrated st cells within 1 hr postinoculation (pi). development was initially by endodyogeny to produce binucleate type i meronts and type i merozoites. division by endodyogeny continued during the 13-day observation period and type i merozoites were the developmental stages most abundant at observation periods >3 days pi. mutinucleate ty ... | 1998 | 9645875 |
| intestinal parasites in swine in the nordic countries: prevalence and geographical distribution. | in denmark (dk), finland (fin), iceland (i), norway (n), and sweden (s), 516 swine herds were randomly selected in 1986-1988. individual faecal analyses (mean: 27.9 per herd) from eight age categories of swine showed that ascaris suum, oesophagostomum spp., isospora suis, and eimeria spp. were common, while trichuris suis and strongyloides ransomi-like eggs occurred sporadically. large fatteners and gilts were most frequently infected with a. suum with maximum prevalences of 25-35% in dk, n and ... | 1998 | 9650867 |
| [effect of a monoinfection with isospora suis and its treatment using halofuginone and lasalocid on breeding achievement, digestion coefficients and composition of whole body cadavers of early weaned piglets]. | | 1981 | 7213333 |
| experimental infection of swine by isospora suis biester 1934 for species confirmation. | a survey of isospora suis performed in 177 faecal samples from 30 swine farms detected thin wall type i. suis oocysts in seven samples. this type of oocyst measuring 23.9 by 20.7 microns had a retracted thin wall similar to that of the genus sarcocystis. this type of oocysts, isolated from four different faecal samples, was inoculated in four-five-days-old piglets free of contamination in order to verify the life cycle and pathogenicity of the species. the pigs were kept in individual metal cage ... | 1998 | 9921315 |
| the prevalence of isospora suis and strongyloides ransomi in suckling piglets in the netherlands. | faecal samples from suckling piglets from 113 litters on 25 farms in the netherlands were examined to study the prevalence of gastrointestinal parasites, in particular strongyloides ransomi and isospora suis. s. ransomi was not found but i. suis was demonstrated in 17 of the 25 farms and in 41 out of 77 litters of these farms (53%). no other parasites were found. on the basis of these results, a longitudinal study on the incidence and significance of i. suis was performed on 10 farms (5 litters/ ... | 1994 | 7740744 |
| occurrence of isospora suis in larger piglet production units and on specialized piglet rearing farms. | mixed fecal samples of 264 litters from five piglet production farms (155-238 sows/farm) were investigated three times during the suckling period for the occurrence of isospora suis over the period of 1 year. on all five farms isopora suis was found to be a common endoparasite with infection rates being highest in litters of 3-4 weeks of age. by the end of the third investigation period the cumulative infection rate was 53.8% of the litters ranging from 20.0% to 81.5% for the single farms. durin ... | 1999 | 10384903 |
| parasites in cross-bred pigs in the upper east region of ghana. | a cross-sectional study was carried out in the upper east region (uer) of ghana in order to estimate the prevalence of parasitic infections in local cross-bred pigs. out of 60 villages with a human population of 200-1000 inhabitants, 10 villages were randomly selected for the study. the number of pigs varied from 50 to 200 pigs per village. in total 259 faecal samples from growers were collected and examined. ninety-one percent of the animals excreted parasite eggs. among these the prevalence of ... | 1999 | 10628701 |
| [endoparasites in swine in different age groups and management systems]. | in different age groups endoparasites are of varying importance. sows are predominantly infected with gastrointestinal strongyles (mainly oesophagostomum spp.), ascaris suum and eimeriae, to a lesset extent with trichuris suis, hyostrongylus rubidus, strongyloides ransomi or isospora suis, while in suckling piglets i. suis and occasionally s. ransomi frequently occur. weaners and fatteners are infected with coccidia and gastrointestinal strongyles, later with a. suum. whipworms are also found oc ... | 2000 | 10816911 |
| immunity against isospora suis in nursing piglets. | | 2000 | 11068822 |
| porcine neonatal coccidiosis. | coccidia were identified in intestinal sections from 82 piglets comprising 37 consignments from 34 farms, and represented a yearly increasing incidence in the three years 1978 to 1980. piglets were primarily from medium to large farms with intensive, continuous-farrowing, confinement-rearing programs. piglets, usually five days to 15 days old, had yellow, fluid diarrhea, became unthrifty and sometimes died. in six piglets from two farms, a green, adherent, fibrinonecrotic membrane was seen throu ... | 1981 | 7343074 |
| diarrhoea in nursing piglets associated with coccidiosis: prevalence, microscopic lesions and coexisting microorganisms. | a retrospective study was made of natural infections with isospora suis in nursing piglets, recorded from april 1994 to may 1997, to determine the prevalence, microscopical lesions and other microorganisms associated with coccidiosis. one hundred and five (17.3 per cent) of the 605 nursing piglets submitted from 304 pig farms were diagnosed positive for coccidiosis. the affected piglets were from seven to 20 days old, with a mean age of 11.1 days. coccidiosis occurred in each year but the incide ... | 1998 | 9807791 |
| autofluorescence microscopy for the detection of nematode eggs and protozoa, in particular isospora suis, in swine faeces. | parasites from swine faeces were examined for autofluorescence. oocysts of eimeria polita, e. scabra and isospora suis, cysts of balantidium coli and eggs of oesophagostomum dentatum, strongyloides ransomi and trichuris suis (but not those of ascaris suum) emitted light after excitation with uv light. i. suis oocyst counts in mcmaster chambers utilising autofluorescence were compared to those from conventional bright field microscopy. similarly, faecal smears containing i. suis were examined usi ... | 2001 | 11403385 |
| role of acquired immunity and natural age resistance on course of isospora suis coccidiosis in nursing piglets. | thirty-two piglets from three litters were experimentally inoculated with 200000 sporulated oocysts of isospora suis at 3 days of age and/or rechallenged at 19 days of age or primary inoculated at 19 days of age, to compare the role of acquired immunity and natural age resistance on the course of coccidiosis. twelve piglets were not inoculated and served as a control. following challenge, the signs of coccidiosis characterised by clinical symptoms, oocysts shedding and weekly weights were simila ... | 1999 | 10321581 |
| lectin histochemistry for glycoconjugates in the small intestines of piglets naturally infected with isospora suis. | composition of glycoconjugates was examined in small intestines naturally infected with isospora suis in preweaned pigs by use of 21 biotinylated-labeled lectins with avidin-biotin-peroxidase complex method. as compared with control pig, staining of 18 lectins altered in jejunal villus brush border and goblet cells of pigs naturally infected with i. suis. these results indicate that i. suis infection alters carbohydrate residues on the jejunal intestines. | 2003 | 12679572 |
| parasitological and morphological findings in porcine isosporosis after treatment with symmetrical triazintriones. | neonatal porcine isosporosis is known to cause serious economic losses in piglet farms by causing severe enteritis with dehydration, weight loss and reduced development in the affected animals, predominantly during the first weeks of life. in the present study, piglets experimentally infected with isospora suis were treated with bay vi 9143, a symmetrical triazintrione, at different days post-infection. as shown by clinical and pathological examinations, bay vi 9143 is effective against the asex ... | 2003 | 12856173 |
| population biology studies on isospora suis in piglets. | | 2003 | 12928890 |
| studies on the efficacy of toltrazuril, diclazuril and sulphadimidine against artificial infections with isospora suis in piglets. | | 2003 | 12928891 |
| the role of isospora suis in the ethiology of diarrhoea in suckling piglets. | | 2003 | 12928892 |
| detection of isospora suis (biester and murray 1934) in piglet faeces--comparison of microscopy and pcr. | | 2004 | 15107041 |
| survey of intestinal parasites in pigs from intensive farms in guangdong province, people's republic of china. | the prevalence of intestinal parasites was investigated in intensive pig farms in guangdong province, china between july 2000 and july 2002. faecal samples from 3636 pigs (both sexes and five age groups) from 38 representative intensive pig farms employing different parasite control strategies were examined for the presence of helminth ova and protozoan oocysts, cysts and/or trophozoites using standard techniques. of the 3636 pigs sampled, 209 (5.7%) were infected with trichuris suis, 189 (5.2%) ... | 2004 | 15710534 |
| occurrence of isospora suis in germany, switzerland and austria. | nationwide surveys for the occurrence of isospora suis were carried out in germany, austria and switzerland including a questionnaire regarding herd size, health status and management practices and a coccidiosis sampling kit for pooled faecal samples from litters of suckling piglets. a total of 184 veterinary practices participated in the survey and returned 1745 samples (331 kits) from 324 farms in the north (n = 98), south (n = 84), centre/east (n = 42) and west (n = 10) of germany, austria (n ... | 2005 | 15752269 |
| effects of toltrazuril on the growth of piglets in herds without clinical isosporosis. | isospora suis is a widely prevalent and economically important parasite. the antiprotozoal compound, toltrazuril, was given as a single treatment to piglets without clinical signs in 10 herds. the daily weight gain (dwg) and mortality between the treatment date and weaning was compared on each herd in 10 treated and 10 control litters. the faeces of control litters were examined for oocyst excretion. in six herds, no oocysts were detected and treatment had no effect on dwg or mortality. in four ... | 2007 | 16122955 |
| isospora suis: an experimental model for mammalian intestinal coccidiosis. | piglets experimentally infected with 10,000 oocysts of isospora suis in three identical trials (n = 50) were examined clinically and coproscopically from 5 to 11 days post-infection (d.p.i.), weighed in weekly intervals until the fourth week of life and compared to age-matched asymptomatic controls (n = 17). furthermore, 17 infected piglets were histologically examined on days 5-14 p.i. infected animals had a significantly lower weight gain than the controls and showed diarrhoea throughout, with ... | 2006 | 16323027 |
| [comparison of different methods for examining the feces of suckling piglets for isospora suis]. | a highly sensitive diagnostic method is of great importance for detection of i. suis (isospora suis). in the present study ksfv (combined sedimentation-flotation method) using different flotation solutions (nacl, znso4, nacl-sucrose) was investigated regarding sensitivity, costs and expenditure of human labour in comparison to fv (flotation method) using nacl-sucrose solution and to am (autofluorescence microscopy). ninety faecal samples were examined. using am, oocysts were detected in 83.3% of ... | 2006 | 17009709 |
| pcr-based differentiation of three porcine eimeria species and isospora suis. | isospora suis and eimeria are frequent coccidian parasites of pigs. the unsporulated oocysts of eimeria species and of i. suis are difficult to differentiate. therefore, a species-specific pcr was developed. pcr products were amplified from eimeria polita, eimeria porci, and eimeria scabra using primers from the conserved 18s rrna regions and were subsequently sequenced. based on variable sequence regions, primers were constructed for the differentiation of the three eimeria species and i. suis. ... | 2001 | 11163694 |
| control of piglet coccidiosis by chemical disinfection with a cresol-based product (neopredisan 135-1). | isospora suis is a common pathogen in piggeries and one of the main causative agents of scours in suckling piglets. besides specific treatment, optimised hygiene including chemical disinfection is considered essential in the control of isosporosis. the suitability of the cresol-based product neopredisan 135-1(r) (np) to inactivate oocysts in vitro and to reduce infection pressure in commercial piggeries was evaluated. under in vitro conditions, np at a final concentration of 2 or 4% induced lysi ... | 2007 | 17364163 |
| the role of isospora suis as a pathogen in conventional piglet production in germany. | in order to evaluate the prevalence of isospora suis in conventional piglet production in germany, pooled faecal samples from 327 pig litters from 18 pig production units (20-320 sows each) were examined. at least 10 litters from each farm were investigated. i. suis was present on 83% of the farms and 42.5% of the litters, the infection rate being highest in the third week of age (48.2%). i. suis was found more frequently in samples of diarrhoea than in firm faeces (49.2% compared to 22.2%). twe ... | 2002 | 12069269 |
| prevalence of isospora suis and eimeria spp. in suckling piglets and sows in poland. | the aim of this investigation was to determine the prevalence of coccidian infections in suckling piglets and sows in poland. the research was carried out in 14 out of 16 polish provinces in the years 2003-2005. the investigation was conducted on three types of farms: large farms (>100 sows), medium farms (25-100 sows) and small farms (<25 sows). diarrhoea of unweaned piglets was observed on all the examined farms. overall, 780 litters of suckling piglets from 104 farms and 267 mother sows were ... | 2007 | 17467906 |
| porcine isosporosis: infection dynamics, pathophysiology and immunology of experimental infections. | isospora suis, an intestinal protozoan parasite of swine, is the causative agent of neonatal coccidiosis, a disease with high morbidity in affected pig-breeding units and consequently of high economic importance. infection leads to damage of the mucosal surface in the jejunum and ileum and to non-haemorrhagic diarrhoea. as a result, weight gain of piglets is reduced and secondary infections with other enteric pathogens may lead to increased mortality. despite its economic and veterinary importan ... | 2007 | 17987356 |
| population dynamics and intra-litter transmission patterns of isospora suis in suckling piglets under on-farm conditions. | the aim of this study was to investigate the intra-litter infection dynamics of isospora suis under natural conditions, and to study any association between parasite transmission and the contamination level of the farrowing pen by applying different interventions in order to reduce the transmission of i. suis infection within the litter. the study was divided in 2 trials including in total 22 litters (254 piglets). the first trial included 4 litters (where standard procedures practiced routinely ... | 2008 | 18021464 |
| prevalence of enteropathogens in suckling and weaned piglets with diarrhoea in southern germany. | faecal samples from suckling (n = 205) and weaned piglets (n = 82) with diarrhoea from 24 farms in southern germany were examined for shedding of important metazoic parasitic, viral and bacterial pathogens using culture, microscopic and electronmicroscopic methods. escherichia coli isolates were tested further for the enterotoxin genes est-ia and elt-i by colony blot hybridization. isospora suis was diagnosed in 26.9% and cryptosporidium parvum in 1.4% of the piglets investigated. the proportion ... | 2001 | 11315526 |
| molecular identification and prevalence of isospora sp. in pigs in western australia using a pcr-rflp assay. | little is known about the prevalence of isospora in domestic pigs in western australia. a total of 289 pig faecal samples were collected from pre- and post-weaned pigs and sows from 1 indoor and 3 outdoor piggeries located in the south-west region of western australia. faecal samples were screened using a pcr-rflp assay based on the its-1 rdna locus. an overall prevalence of 10.4% (30/289) was identified. isospora was detected in 16.3% (20/123) of pre-weaned animals and 6.4% (10/156) of post-wea ... | 2008 | 18601925 |
| modified flotation method with the use of percoll for the detection of isospora suis oocysts in suckling piglet faeces. | the modification of flotation method for the examination of diarrhoeic piglet faeces for the detection of isospora suis oocysts was elaborated. the method was based on removing fractions of fat from the sample of faeces by centrifugation with a 25% percoll solution. the investigations were carried out in comparison to the mcmaster method. from five variants of the percoll flotation method, the best results were obtained when 2ml of flotation liquid per 1g of faeces were used. the limit of detect ... | 2008 | 18602760 |
| toltrazuril and sulphonamide treatment against naturally isospora suis infected suckling piglets: is there an actual profit? | a study was carried out to assess the efficacy and the economic profit of prophylactic treatment against isopsora suis with toltrazuril or with a sulfamethazine/trimethoprim combination in piglets from an intensive pig farm. thirty-one litters were included in study. eight litters were treated once with toltrazuril (20 mg/kg b.w.) at 3 days of age (toltra group); 8 litters were treated with 2 ml/animal of a [corrected] sulphonamide combination (sodium sulfamethazine 250 [dosage error corrected] ... | 2009 | 19457615 |
| porcine coccidiosis--investigations on the cellular immune response against isospora suis. | porcine neonatal coccidiosis is caused by the protozoan isospora suis and affects mainly piglets in the first three weeks of life. high morbidity with diarrhoea and reduced weight gain lead to economic losses, affecting pig-breeding worldwide. infection causes damage of the mucosal surface in the jejunum and ileum and transient non-haemorrhagic diarrhoea. secondary infections with other enteric pathogens may lead to increased mortality. despite its economic and veterinary importance, the immunol ... | 2009 | 19575236 |
| age, not infection dose, determines the outcome of isospora suis infections in suckling piglets. | data from 13 trials involving 124 suckling piglets experimentally infected with isospora suis were evaluated for the effects of infection dose and age on the clinical and parasitological outcome of infection in four different models, infections with 1,000 oocysts on the 1(st) day of life (d.o.l.) (model 1; 9 piglets/3 litters), 1,000 oocysts on the 4(th) d.o.l. (model 2; 25 piglets/11 litters), 1,500 oocysts on the 4(th) d.o.l. (model 3; 40 piglets/20 litters) and 10,000 oocysts on the 4(th) d.o ... | 2009 | 19575237 |
| porcine neonatal coccidiosis in quebec. | intestinal coccidiosis was diagnosed in 110 diarrheic piglets originating from 66 farrowing operations. these piglets had been submitted alive for necropsy early after the beginning of diarrhea, and the diagnosis of coccidiosis was based on the demonstration of coccidial forms in their jejunum by histopathological examination. enteropathogenic strains of escherichia coli were demonstrated in only nine of the 110 piglets while the transmissible gastroenteritis virus and rotavirus were each presen ... | 1982 | 17422160 |