transient protein expression in three pisum sativum (green pea) varieties. | the expression of proteins in plants both transiently and via permanently transformed lines has been demonstrated by a number of groups. transient plant expression systems, due to high expression levels and speed of production, show greater promise for the manufacturing of biopharmaceuticals when compared to permanent transformants. expression vectors based on a tobacco mosaic virus (tmv) are the most commonly utilized and the primary plant used, nicotiana benthamiana, has demonstrated the abili ... | 2009 | 19156736 |
molecular and biological characterization of an isolate of cucumber mosaic virus from glycine soja by generating its infectious full-genome cdna clones. | molecular and biological characteristics of an isolate of cucumber mosaic virus (cmv) from glycine soja (wild soybean), named as cmv-209, was examined in this study. comparison of nucleotide sequences and phylogenetic analyses of cmv-209 with the other cmv strains revealed that cmv-209 belonged to cmv subgroup i. however, cmv-209 showed some genetic distance from the cmv strains assigned to subgroup ia or subgroup ib. infectious full-genome cdna clones of cmv-209 were generated under the control ... | 2014 | 25288998 |
first report of cucumber mosaic virus isolated from wild vigna angularis var. nipponensis in korea. | a viral disease causing severe mosaic, necrotic, and yellow symptoms on vigna angularis var. nipponensis was prevalent around suwon area in korea. the causal virus was characterized as cucumber mosaic virus (cmv) on the basis of biological and nucleotide sequence properties of rnas 1, 2 and 3 and named as cmv-wva. cmv-wva isolate caused mosaic symptoms on indicator plants, nicotiana tabacum cv. xanthi-nc, petunia hybrida, and cucumis sativus. strikingly, cmv-wva induced severe mosaic and malform ... | 2014 | 25289004 |