cytotoxicity of bacillus isolates of b. piliformis, the agent of tyzzer's disease, obtained from various host species, were examined for cytotoxic activity by incubating culture filtrates on brl 3a rat-hepatocyte and 3t3 mouse-fibroblast cell lines. one isolate exhibited cytopathic effects on brl 3a cells, but not on 3t3 cells. three other isolates were strongly cytotoxic for 3t3 cells but only slightly so for brl 3a cells. the remaining three isolates showed no cytotoxicity for either cell line. the cytotoxic pro ...19921625320
cultivation of bacillus piliformis (tyzzer) in mouse fibroblasts (3t3 cells).bacillus piliformis was successfully propagated in a continuous mouse-embryo fibroblast cell line (3t3). 3t3 monolayers were successfully inoculated with a variety of infected materials including yolk sac and liver suspensions, minced yolk sac and liver, and primary chicken-embryo liver-cell cultures. an initial decrease in b. piliformis number was noted after inoculation of the cell layer with 2.6 x 10(5) organisms followed by a peak bacterial count at 48 h. bacillus piliformis remained infecti ...19902353449
a study of mouse strains susceptibility to bacillus piliformis (tyzzer's disease): the association of b-cell function and resistance.tests were conducted on 11 inbred strains of mice and an nih outbred stock. it was found that only the cba/n and c3. cba/n mice (strains deficient in igm production) were highly susceptible to bacillus piliformis infection. susceptibility to infection was determined by induction of typical surface liver lesions and the ability to maintain serial passage without concurrent administration of cortisone. mice deficient in t-cell function (nu/nu/++) were as resistant to bacillus piliformis infection ...19816972467
infectious microorganisms in mice (mus musculus) purchased from commercial pet shops in this study, we investigated the prevalence of infectious microorganisms (viruses, bacteria, fungi and eukaryotic parasites) in mice from different pet shops in germany; such animals may compromise the hygienic integrity of laboratory animal vivaria if private pet holders act as unintended vectors of infections carried by them. house mice sold as pets or feed specimens were purchased from different pet shops and tested for a comprehensive panel of unwanted microorganisms. we found a number of ...201121508117
role of interleukin-6 in determining the course of murine tyzzer's disease.clostridium piliforme is an obligately intracellular bacterium that causes enterohepatic disease in many domestic and laboratory animal species. susceptibility to infection is known to vary with the host immune status, species and strain, but little is known about specific immune mechanisms that regulate this disease. subclinical infection was induced in weanling c. piliforme-susceptible dba/2 or resistant c57bl/6 mice with either a toxic or a non-toxic c. piliforme isolate. hepatic lesions and ...200010670568
experimentally induced tyzzer's disease in mongolian gerbils (meriones unguiculatus).tyzzer's disease was induced in outbred, 3- to 4-week-old female mongolian gerbils (meriones unguiculatus) by oral inoculation of either a yolk sac preparation containing spores or a suspension of infected mouse liver. clinical signs and lesions were similar regardless of the inoculum. nests of bacillus piliformis were seen in follicle associated epithelium of ileal peyer's patches by 24 hours post-inoculation. disease severity peaked at the fifty through seventh days. many animals died during t ...19846716957
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