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identification of novel dna fragments and partial sequence of a genomic island specific of brucella pinnipedialis.since the 1990s, brucella strains have been isolated from a wide variety of marine mammals and were recently recognized as two different species, i.e. brucella pinnipedialis for pinniped isolates and brucella ceti for cetacean isolates. the aim of this study was to identify specific dna fragments of marine mammal brucella strains using a previously described infrequent restriction site-pcr (irs-pcr) method but with three new couples of restriction enzymes applied on a larger panel of marine mamm ...200818514443
prevalence of brucella pinnipediae in healthy hooded seals (cystophora cristata) from the north atlantic ocean and ringed seals (phoca hispida) from svalbard.investigations for brucella-infections were conducted in 29 hooded seals (cystophora cristata) caught between svalbard and greenland (north atlantic ocean; greenland sea) autumn 2002, and from 20 ringed seals (phoca hispida) caught in billefjord, svalbard, spring 2003. all animals were apparently healthy and were caught in their natural habitat. bacteriology on tissue samples from ringed seals was negative, whereas brucella sp. were recovered in tissues from 11 of the 29 hooded seals (38%), with ...200415627521
brucella pinnipedialis hooded seal (cystophora cristata) strain in the mouse model with concurrent exposure to pcb 153.brucellosis, a worldwide zoonosis, is linked to reproductive problems in primary hosts. a high proportion of brucella-positive hooded seals (cystophora cristata) have been detected in the declined northeast atlantic stock. high concentrations of polychlorinated biphenyls (pcbs) have also been discovered in top predators in the arctic, including the hooded seal, pcb 153 being most abundant. the aim of this study was to assess the pathogenicity of brucella pinnipedialis hooded seal strain in the m ...201424534631
experimental challenge of atlantic cod (gadus morhua) with a brucella pinnipedialis strain from hooded seal (cystophora cristata).pathology has not been observed in true seals infected with brucella pinnipedialis. a lack of intracellular survival and multiplication of b. pinnipedialis in hooded seal (cystophora cristata) macrophages in vitro indicates a lack of chronic infection in hooded seals. both epidemiology and bacteriological patterns in the hooded seal point to a transient infection of environmental origin, possibly through the food chain. to analyse the potential role of fish in the transmission of b. pinnipediali ...201627415626
brucella pinnipedialis in hooded seal (cystophora cristata) primary epithelial cells.marine brucella spp. have been isolated from numerous pinniped and cetacean species, but pathological findings in association with infection with brucella pinnipedialis in pinnipeds have been sparse. the capacity of brucellae to survive and replicate within host macrophages underlies their important ability to produce chronic infections, but previous work has shown that b. pinnipedialis spp. are rapidly eliminated from hooded seal (cystophora cristata) alveolar macrophages.201526809981
entrance and survival of brucella pinnipedialis hooded seal strain in human macrophages and epithelial cells.marine mammal brucella spp. have been isolated from pinnipeds (b. pinnipedialis) and cetaceans (b. ceti) from around the world. although the zoonotic potential of marine mammal brucellae is largely unknown, reports of human disease exist. there are few studies of the mechanisms of bacterial intracellular invasion and multiplication involving the marine mammal brucella spp. we examined the infective capacity of two genetically different b. pinnipedialis strains (reference strain; ntct 12890 and a ...201324376851
a review of brucella infection in marine mammals, with special emphasis on brucella pinnipedialis in the hooded seal (cystophora cristata).abstract: brucella spp. were isolated from marine mammals for the first time in 1994. two novel species were later included in the genus; brucella ceti and brucella pinnipedialis, with cetaceans and seals as their preferred hosts, respectively. brucella spp. have since been isolated from a variety of marine mammals. pathological changes, including lesions of the reproductive organs and associated abortions, have only been registered in cetaceans. the zoonotic potential differs among the marine m ...201121819589
mlva-16 typing of 295 marine mammal brucella isolates from different animal and geographic origins identifies 7 major groups within brucella ceti and brucella pinnipedialis.since 1994, brucella strains have been isolated from a wide range of marine mammals. they are currently recognized as two new brucella species, b. pinnipedialis for the pinniped isolates and b. ceti for the cetacean isolates in agreement with host preference and specific phenotypic and molecular markers. in order to investigate the genetic relationships within the marine mammal brucella isolates and with reference to terrestrial mammal brucella isolates, we applied in this study the multiple loc ...200919619320
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