| detection of methanogens and proteobacteria from a single cell of rumen ciliate protozoa. | rumen ciliate-associated bacteria and methanogenic archaea were analyzed by a 16s rrna gene retrieved from a single cell of polyplastron multivesiculatum, isotricha intestinalis, and ophryoscolex purkynjei. rumen fluid was taken from a ruminally fistulated goat to prepare a ciliate fraction. ciliate mixtures were incubated under mixtures of antibiotics for 48 h to eliminate extracellular bacteria. individual cells of rumen ciliates were selected under microscopic observation after fixation with ... | 2004 | 15754246 |