| genotypic and phenotypic diversity of lactococcus lactis isolates from batzos, a greek pdo raw goat milk cheese. | the genotypic and phenotypic variability of 40 lactococcus lactis isolates obtained from three cheese-making trials of batzos cheese made one in each, winter, spring and summer was investigated. rapd-pcr, plasmid profiling and pfge were used to study the genetic variability and distinguish closely related isolates. results showed a high degree of heterogeneity among strains. according to pfge data, all strains except one were clustered together (at a similarity level of approximately 50%) with t ... | 2007 | 17241681 |
| stability of microbial communities in goat milk during a lactation year: molecular approaches. | the microbial communities in milks from one herd were evaluated during 1-year of lactation, using molecular methods to evaluate their stability and the effect of breeding conditions on their composition. the diversity of microbial communities was measured using two approaches: molecular identification by 16s and 18s rdna sequencing of isolates from counting media (two milks), and direct identification using 16s rdna from clone libraries (six milks). the stability of these communities was evaluat ... | 2007 | 17604934 |
| risk factors for intramammary infections and relationship with somatic-cell counts in italian dairy goats. | routine examination of milk was performed on five herds of lactating goats in northern italy as part of a milk quality-monitoring program in the year 2000. as part of the study, aseptic samples of foremilk were collected monthly from both half udders during the entire lactation for 305 goats, resulting in a total of 4571 samples. the samples were tested with cytological and bacteriological analyses to evaluate the relationship between mammary infections and somatic-cell count (scc; fossomatic (t ... | 2005 | 15907567 |
| characterization of lactic acid bacteria isolated from bukuljac, a homemade goat's milk cheese. | the bukuljac cheese is traditionally homemade cheese, produced from heat-treated goat's milk without the addition of any bacterial starter culture. the presence of lactic acid bacteria (lab) in bukuljac cheese has been analyzed by using a polyphasic approach including microbiological and molecular methods such as rep-pcr with (gtg)5 primer. lactobacillus paracasei subsp. paracasei represents a dominant strain in the microflora of analyzed cheese. out of 55 gram-positive and catalase-negative iso ... | 2008 | 18177967 |
| histamine formation by enterococci isolated from home-made goat cheeses. | a survey was made of the histamine-producing capability of enterococci isolated from goat cheeses. the strains, 130 streptococcus faecium and 106 s. faecalis, were grown in trypticase soy broth histidine medium (tsbh) at 35 degrees c for 24 h and the histamine formed was determined by fluorometry. forty-one (31.5%) of the s. faecium strains and 2 (1.9%) of the s. faecalis strains produced histamine. the largest amount detected was 4.0 micrograms histamine/ml tsbh. compared with the amounts of hi ... | 1988 | 3152802 |
| lactic acid bacteria isolated from ethnic preserved meat products of the western himalayas. | we used culture- and molecular-biology-based methods to investigate the diversity of lactic acid bacteria (lab) in the ethnic chevon (goat) meat products chartayshya, jamma and arjia of the western himalayas. in six chartayshya, six jamma and four arjia samples, lab were the predominant microbial component involved in the fermentation of these samples, and the total lab population in arjia (7.8-á-¦-á0.1-álog cfu-ág(-1); mean-á-¦-ásd) was significantly higher (p-á<-á0.05) than in chartayshya (6.9 ... | 2011 | 21839380 |
| occurrence and preliminary study of antimicrobial resistance of enterococci isolated from dairy goats in spain. | the present study performed in spain was designed to investigate the occurrence and antimicrobial resistance of enterococci in faecal and bulk tank milk samples from 222 healthy murciano-granadina dairy goats reared on 12 farms. enterococci were isolated in 54.5% and 63.6% of the faecal and bulk tank milk samples, respectively. enterococci were detected more frequently from goat kids (70%) than from replacement animals (44.4%) and adults (56.4%). seven species were found in the faecal samples bu ... | 2006 | 16682094 |
| demonstration of the phenomena of microbial persistence and reversion with bacterial l-forms in human embryonic kidney cells. | a series of experiments was undertaken in which human embryonic kidney (hek) fibroblasts were infected with either relatively stable or stable l-forms of streptococcus faecalis. the infected cells were maintained by splitting over long periods (1 to 2 months) while samples were withdrawn for culture and electron microscopic studies. relatively stable s. faecalis l-forms could be cultured from infected cells only during the first week after infection, although l-form-like material was frequently ... | 1974 | 4214785 |
| detection of biogenic amine producer bacteria in a typical italian goat cheese. | the aim of this study was to research decarboxylating bacterial strains and biogenic amine content in a typical italian goat cheese (robiola di roccaverano). the study was performed on fresh and ripened samples of goat cheese manufactured from industrial and artisanal producers. sixty-seven bacterial strains isolated showed decarboxylating activity, and enterococcus faecalis was the most widespread decarboxylating species in all artisanal and industrial products. pediococcus acidilactici and ent ... | 2008 | 18236686 |
| enterocin as-48rj: a variant of enterocin as-48 chromosomally encoded by enterococcus faecium rj16 isolated from food. | the bacteriocinogenic strain rj16 isolated from goat cheese has been identified as enterococcusfaecium by species-specific pcr, dna-rrna hybridization and rdna sequencing. purified bacteriocin from strain rj16 is a carboxypeptidase a-resistant peptide with a molecular mass (7125 da) very close to the cyclic peptide enterocin as-48. bacteriocin from strain rj16 and as-48 show identical antibacterial spectra, although the former is slightly less active on strains of listeria monocytogenes and baci ... | 2005 | 16094865 |
| phenotypic and genetic diversity of enterococci isolated from italian cheeses. | in the present study, 124 enterococcal strains, isolated from traditional italian cow, goat and buffalo cheeses, were characterized using phenotypic features and randomly amplified polymorphic dna polymerase chain reaction (rapd-pcr). the rapd-pcr profiles obtained with four primers and five different amplification conditions were compared by numerical analysis and allowed an inter- and intraspecific differentiation of the isolates. whole cell protein analysis by sodium dodecyl sulfate polyacryl ... | 2001 | 11504393 |