| genetic diversity within cryptosporidium parvum and related cryptosporidium species. | to assess the genetic diversity in cryptosporidium parvum, we have sequenced the small subunit (ssu) rrna gene of seven cryptosporidium spp., various isolates of c. parvum from eight hosts, and a cryptosporidium isolate from a desert monitor. phylogenetic analysis of the ssu rrna sequences confirmed the multispecies nature of the genus cryptosporidium, with at least four distinct species (c. parvum, c. baileyi, c. muris, and c. serpentis). other species previously defined by biologic characteris ... | 1999 | 10427023 |
| identification of genotypes of cryptosporidium parvum isolates from ferrets in japan. | recent molecular studies show that cryptosporidium parvum is composed of at least eight genotypes including zoonotic genotypes. therefore, it is important to screen the genotypes of the isolates harbored in animals for the control of human cryptosporidiosis. the ferret is a popular pet, but also a reservoir of cryptosporidium. since the infectivity of zoonotic genotypes in ferrets remains unclear, there is a possibility these hosts harbor zoonotic genotypes. in the present study, we examined the ... | 2003 | 12632159 |
| molecular fingerprinting of cryptosporidium oocysts isolated during water monitoring. | we developed and validated a pcr-based method for identifying cryptosporidium species and/or genotypes present on oocyst-positive microscope slides. the method involves removing coverslips and oocysts from previously examined slides followed by dna extraction. we tested four loci, the 18s rrna gene (n18sdiag and n18sxiao), the cryptosporidium oocyst wall protein (cowp) gene (stn-cowp), and the dihydrofolate reductase (dhfr) gene (by multiplex allele-specific pcr), for amplifying dna from low den ... | 2006 | 16885295 |
| first report of cryptosporidium parvum 'ferret' genotype in american mink (mustela vison shreber 1777). | a total of 51 faecal samples from wild and farmed mink were analysed by a direct immunofluorescence antibody test. cryptosporidium oocysts were identified in eight, apparently healthy, farmed american mink (mustela vison). the isolates were identified as cryptosporidium parvum 'ferret' genotype by pcr-rflp and sequencing analysis of a 341-base-pair fragment of the cryptosporidium oocyst wall protein (cowp) gene. this is the first report of cryptosporidium in american mink. | 2007 | 17111177 |
| cryptosporidium spp. in wild, laboratory, and pet rodents in china: prevalence and molecular characterization. | to understand the prevalence of cryptosporidium infection in rodents in china and to assess the potential role of rodents as a source for human cryptosporidiosis, 723 specimens from 18 rodent species were collected from four provinces of china and examined between august 2007 and december 2008 by microscopy after using sheather's sugar flotation and modified acid-fast staining. cryptosporidium oocysts were detected in 83 specimens, with an overall prevalence of 11.5%. phodopus sungorus, phodopus ... | 2009 | 19820152 |