| post-epizootic surveys of waterfowl for duck plague (duck virus enteritis). | surviving birds from nine duck plague outbreaks in urban and confined waterfowl were sampled for duck plague (dp) virus and dp antibody during 1979-86. duck plague virus was found in combined oral and cloacal swabs of birds from three outbreaks, and dp-neutralizing antibody was demonstrated in some birds from all nine outbreaks. greater prevalence of dp antibody and higher titers were found in survivors from confined populations than from free-flying urban populations. free-flying waterfowl from ... | 1988 | 2849402 |
| the susceptibility and response of wild waterfowl to duck plague virus. | | 1969 | 4357476 |
| serologic and immunologic response of ducks to inactivated and attenuated duck plague virus. | | 1969 | 5391212 |
| increased cell culture incubation temperatures for duck plague virus isolation. | | 1981 | 7271660 |
| a plaque assay for duck plague virus. | a plaque assay for duck plague virus was developed for a chicken embryo-adapted virus and a duck lethal virus and used to determine the identity of these viruses. using the plaque inhibition neutralization test, duck plague virus was differentiated from newcastle disease, fowl plague, and duck hepatitis viruses. the plaque morphology is described. | 1968 | 15846902 |
| molecular cloning and sequence analysis of the duck enteritis virus ul5 gene. | duck enteritis virus (dev) is a herpesvirus that causes an acute, contagious, and fatal disease. in the present article, the dev ul5 gene was cloned and sequenced from a vaccine virus. according to the consensus sequence of herpesvirus ul5 and ul3 gene degenerate oligonucleotide primers were designed and were used in the polymerase chain reaction (pcr) to amplify dna products with 4577 bp in size. dna sequence analysis revealed a 2568 bp open reading frame (orf) encoding a 855 amino acid polypep ... | 2008 | 18582977 |
| an immunocytochemical study on the sequential tissue distribution of duck plague virus. | primary replication and tissue distribution of duck plague (duck virus enteritis) virus in domestic ducks following oral infection were studied by an avidin-biotin-peroxidase complex (abc) method of immunoperoxidase staining. the virus replicated primarily in the mucosa of the digestive tract, especially in the oesophagus as early as 24 h after infection, then spread to the bursa of fabricius, thymus, spleen and liver. the epithelial cells and macrophages of these organs were the principal site ... | 1995 | 18645775 |
| unique sequence characteristics of genes in the leftmost region of unique long region in duck enteritis virus. | our objective was to further understand the evolutionary position of duck enteritis virus (dev) in the family herpesviridae and to determine the genomic structure in the leftmost region of the unique long region (ul) of dev. | 2009 | 19707022 |
| development and application of a reverse transcriptase polymerase chain reaction to detect chinese isolates of duck hepatitis virus type 1. | we developed a reverse transcriptase polymerase chain reaction (rt-pcr) method for the detection of duck hepatitis virus type 1 (dhv-1) in the tissues of infected and clinically affected ducks and in chick and duck embryos. we found the assay to be effective in detecting the virus in china, where it is being used in studies on the epidemiology of the disease. we applied this simple and rapid diagnostic method to the detection of dhv isolates grown in chick and duck embryos and in tissues obtaine ... | 2009 | 18706944 |
| the pathogenesis of duck virus enteritis in experimentally infected ducks: a quantitative time-course study using taqman polymerase chain reaction. | duck virus enteritis is an acute and contagious herpesvirus infection of duck, geese and swans with high morbidity and mortality. the kinetics of viral dna loads and immunohistochemical localization of virulent duck enteritis virus, as well as histopathological examination in various tissues of ducks following oral infection, were investigated. the time course for the appearance of viral antigen and tissue lesions in various tissues was coincident with the levels of duck enteritis virus at the v ... | 2008 | 18568657 |
| molecular characterization of the herpes simplex virus 1 (hsv-1) homologues, ul25 to ul30, in duck enteritis virus (dev). | a 16.6-kilo-base pair (kb) sequence was amplified from the duck enteritis virus (dev) clone-03 strain genome using 'targeted gene walking polymerase chain reaction (pcr)'. seven complete open reading frames (orfs) were predicted, and designated herpes simplex virus 1 (hsv-1) homologues, unique long (ul) 25, ul26, ul26.5, ul27, ul28, ul29, and ul30. sequence analysis revealed that the arrangement of seven genes in dev clone-03 strain was collinear to that from hsv-1. in addition, mrna transcripti ... | 2007 | 17706377 |
| a monoclonal antibody to inclusion body disease of cranes virus enabling specific immunohistochemistry and competitive elisa. | inclusion body disease of cranes (ibdc) herpesvirus kills some infected cranes and persists in convalescent animals. to enable further study and rapid identification of carrier animals, we developed a monoclonal antibody (mab) to ibdc virus and used it in immunohistochemistry and a competitive enzyme-linked immunosorbent assay (elisa). we used conventional techniques to make murine mabs directed against ibdc virus purified from infected duck embryo cells. hybridomas reacting in an elisa with ibd ... | 1997 | 9454913 |
| virulence of six strains of duck plague virus in eight waterfowl species. | susceptibility of new world waterfowl to the lake andes strain of duck plague virus (dpv) was assessed by intramuscular inoculation of adult muscovies (cairina moschata), mallards (anas platyrhynchos), canada geese (branta canadensis), wood ducks (aix sponsa), redheads (aythya americana), gadwalls (anas strepera), blue-winged teal (anas discors), and pintails (anas acuta). the relative virulence of dpv strains isolated from five united states and one canadian location was established in muscovie ... | 1996 | 8827671 |
| improvement in plaquing methods for the enumeration of anatid herpesvirus (duck plague virus). | the holland strain of anatid herpesvirus (ahv) was 2-to 10-fold more efficiently plaqued under liquid medium or semisolid medium containing methylcellulose than in media containing agar, agarose, or nobel agar. virus adsorption was complete in 45 min, with the maximum virus titers obtained under liquid medium at 48 h postinfection. the ahv dose-response curve was linear. pekin duck embryo cultures were more efficient than ccl-141 in plaque response and gave the maximum virus yield per cell. viru ... | 1980 | 7251330 |
| cytoplasmic origin of an intranuclear dna virus (duck plague virus). | electron microscopic and autoradiographic studies have revealed that the assembly and maturation of duck plague virus (dpv, an intranuclear dna virus) occur both in the nucleus and in the cytoplasm. a dense cytoplasmic matrix (tentatively called viroplast) is the basic organelle in which the assembly of nucleocapsids occurs. at the early stage of virion maturation, dense particles, approximately 40 nm in size, appear and become assembled nucleocapsid structures later on. as capsids become mature ... | 1982 | 7201675 |
| the epidemiology of avian herpesviruses in veterinary medicine. | there are ten avian herpesviruses, which have been isolated from eight orders. six of these are of veterinary importance: pacheco's parrot disease virus, pigeon herpesvirus, duck plague virus, infectious laryngotracheitis virus, herpesvirus of turkeys and marek's disease virus. the knowledge on the epidemiology of each virus and the disease it causes is discussed. features in common to infections with most avian herpesviruses are: infection is persistent in individuals and ubiquitous in populati ... | 1982 | 6299838 |
| vertical transmission of duck plague virus (dpv) by apparently healthy dpv carrier waterfowl. | duck plague virus (dpv) was transmitted vertically in muscovy, pekin, and mallard ducks that were persistently infected with the la-sd-73, msn-wi-77, or co-wi-73 isolates of dpv. the effects of vertical transmission on the fertility and hatchability of eggs laid by dpv carrier ducks varied with the dpv isolate and duck species. fertility was reduced significantly only in eggs laid by msn-wi-77 virus carrier pekin and muscovy ducks. the hatchability of eggs laid by dpv carrier mallards and muscov ... | 1981 | 6279069 |
| isolation of an apathogenic immunogenic strain of duck enteritis virus from waterfowl in california. | a herpesvirus isolated from waterfowl dying of duck enteritis (de) was tentatively designated the sheridan-83. it was serologically related to the original holland and lake andes (la) strains of duck enteritis viruses (dev). other biological characteristics indicated that the sheridan-83 was more closely related to the holland strain than to the la virus. the sheridan-83 was nonpathogenic to ducks, and ducks inoculated with this virus developed resistance to challenge with the virulent strain la ... | 1984 | 6091604 |
| pathological and immunological study of goose embryos experimentally infected with duck plague virus. | a total of 240 embryonated goose eggs obtained from two susceptible flocks were used. half of the eggs were inoculated into the allantoic cavity with a virulent strain (7593) of duck plague virus isolated from an acute outbreak, and the other half were inoculated with the attenuated vaccine virus (kapevac). ten, 100 or 1000 cpu/0.1 ml virus were given on days 12 and 20 of incubation. embryos that died and surviving embryos killed at 5-day intervals were examined by light and electron microscopy. ... | 1990 | 1966126 |
| ultrastructural characterization and hepatic pathogenesis of duck plague virus. | six-week-old white pekin ducks were inoculated intravenously with duck plague virus (dpv) isolated from wild waterfowl. the virus replicated in hepatic macrophages, hepatocytes, and bile duct epithelium. in ultrathin sections, herpes-like nucleocapsids and virions were found respectively in the nucleus and cytoplasm of infected cells. typical herpesviral capsids and virions were seen in negatively-stained preparations of duck embryo fibroblasts. antibodies against holland-attenuated strain of dp ... | 1976 | 176971 |
| development and evaluation of an immunochromatographic strip test based on the recombinant ul51 protein for detecting antibody against duck enteritis virus. | duck enteritis virus (dev) infection causes substantial economic losses to the worldwide duck-producing areas. the monitoring of dev-specific antibodies is a key to evaluate the effect of dev vaccine and develop rational immunization programs. thus, in this study, an immunochromatographic strip (ics) test was developed for detecting dev serum antibodies. | 2010 | 20946624 |
| transcription phase, protein characteristics of dev ul45 and prokaryotic expression, antibody preparation of the ul45 des-transmembrane domain. | some ul45 gene function of herpesvirus was reported. while there was no any report of the duck enteritis virus (dev) ul45 protein as yet. | 2010 | 20843372 |
| expressing gk gene of duck enteritis virus guided by bioinformatics and its applied prospect in diagnosis. | duck viral enteritis, which is caused by duck enteritis virus (dev), causes significant economic losses in domestic and wild waterfowls because of the high mortality and low egg production rates. with the purpose of eliminating this disease and decreasing economic loss in the commercial duck industry, researching on glycoprotein k (gk) of dev may be a new kind of method for preventing and curing this disease. because glycoproteins project from the virus envelope as spikes and are directly involv ... | 2010 | 20663161 |
| characterization of the duck plague virus ul35 gene. | previous study has demonstrated that the duck plague virus (dpv) ul35 gene can be expressed as a recombinant fusion protein, and the prepared antiserum has a high reactivity and specificity against the purified recombinant protein. in the present study, to elucidate the properties and functions of its encoding protein, the ul35 gene product (vp26) was identified by using the prepared rabbit polyclonal antiserum. | 2010 | 20606463 |
| propagation of vaccine strain of duck enteritis virus in a cell line of duck origin as an alternative production system to propagation in embryonated egg. | duck virus enteritis (dve) also known as duck plague, is a viral infection of ducks caused by duck enteritis virus (dev). the control of the disease is mainly done by vaccination with a chicken embryo-adapted live virus that is known to be poorly immunogenic and affords partial protection. further, the risk of harboring other infectious agents in the embryo particularly the deadly and zoonotic avian influenza virus is also high. in this paper, we report propagation of a chicken embryo-adapted va ... | 2010 | 20227293 |
| characterization of synonymous codon usage bias in the duck plague virus ul35 gene. | the aim was to identify the codon usage bias between the newly identified duck plague virus (dpv) ul35 gene (genbank accession no. ef643558) and the ul35-like genes of 27 other reference herpesviruses. | 2009 | 19672100 |
| identification and characterization of the duck enteritis virus ul51 gene. | compared to the ul51 gene of other alphaherpesviruses, the duck enteritis virus (dev) ul51 gene contains ten conserved motifs and has a close evolutionary relationship with members of the genus mardivirus. the dev ul51 gene product was identified using a rabbit polyclonal antiserum raised against a 6-his-ul51 fusion protein expressed in escherichia coli as a 34-kda protein. western blotting and rt-(real time) pcr analysis of dev-infected cells showed that the protein was produced at the late sta ... | 2009 | 19517212 |
| development and evaluation of an antigen-capture elisa for detection of the ul24 antigen of the duck enteritis virus, based on a polyclonal antibody against the ul24 expression protein. | an antigen-capture enzyme-linked immunosorbent assay (ac-elisa) method was developed for the efficient detection of the ul24 antigen of the duck enteritis virus (dev) using polyclonal antibodies. ducks and rabbits were immunized, respectively, with expressed ul24 recombinant protein. the igg antibodies against ul24 from ducks and rabbits were purified and used as the capture antibodies. the specificity of the optimized ac-elisa was evaluated by use of dev, duck hepatitis virus (dhv), duck hepati ... | 2009 | 19467266 |
| expression and characterization of the ul31 protein from duck enteritis virus. | previous studies indicate that the ul31 protein and its homology play similar roles in nuclear egress of all herpesviruses. however, there is no report on the ul31 gene product of dev. in this study, we expressed and presented the basic properties of the dev ul31 product. | 2009 | 19208242 |
| purification of anatid herpesvirus 1 particles by tangential-flow ultrafiltration and sucrose gradient ultracentrifugation. | anatid herpesvirus 1 (ahv-1) infection causes substantial economic losses to the world-wide waterfowl production. however, little is known about the efficient method used to study the purification of ahv-1 and the negative staining morphology of the purified virus particles. this lack of knowledge is one of the important factors that have affected the progress of research studies on ahv-1 molecular virology to such an extent that they are lagging far behind those on other members of the same fam ... | 2009 | 19152808 |
| rapid diagnosis of duck plagues virus infection by loop-mediated isothermal amplification. | duck virus enteritis is a serious disease among farmed and free-living ducks (anatidae) and a constant threat to the commercial duck industry in china. in this study, a loop-mediated isothermal amplification (lamp) assay was developed to rapidly detect and diagnose duck plague virus (dpv) in both farmed and wild waterfowl, and compared with polymerase chain reaction (pcr) method and real-time pcr method in accuracy, sensitivity and specificity. a set of four specific primers was successfully des ... | 2009 | 19117583 |
| absence of neutralising antibodies to duck plague virus in the commercial duck and goose populations in west germany (1980-1985). | commercial duck and goose serum samples were examined in a viral neutralisation test in chicken embryo fibroblast cultures for the presence of specific antibodies against duck plague virus. all 2256 serum samples tested, which originated from different locations in west germany from 1980 to spring 1985, showed neutralising titres of less than 1.0 (log2 ) and were considered to be negative. | 1986 | 18766504 |
| expression and characterization of recombinant vp19c protein and n-terminal from duck enteritis virus. | previous studies have indicated that the vp19c protein and its homology play similar roles in capsid assembly of all alphaherpesvirus subfamily. however, there is no report on the vp19c protein of duck enteritis virus (dev). in this study, we expressed the dev vp19c protein and presented its antigenic properties. moreover, we developed polyclonal antibody against the vp19c protein and characterized it. | 2011 | 21349183 |
| detection of duck plague virus antigen in tissues by immunoperoxidase staining. | an avidin-biotin-peroxidase method of immunoperoxidase staining was successfully adapted for detection of duck plague virus antigen in formalin-fixed, paraffin-embedded tissue sections of the liver and spleen of experimentally infected domestic ducks. positive staining was localized mostly in the nucleus but was also present in the cytoplasm of a few hepatocytes and von kupffer cells of the liver, and lymphocytes and reticular cells of the spleen. | 1993 | 18671026 |
| expression and immunohistochemical distribution of duck plague virus glycoprotein ge in infected ducks. | to determine the distribution of duck plague virus (dpv) ge protein in paraformaldehyde-fixed, paraffin-embedded tissues of experimentally dpv-infected ducks, an indirect immunoperoxidase assay was established to detect glycoprotein e (ge) protein for the first time. the rabbit anti-his-ge serum, raised against the recombinant his-ge fusion protein expressed in escherichia coli bl21 (de3), was prepared and purified. western blotting and indirect immunofluorescence analysis showed that the anti-h ... | 2011 | 21500643 |
| expression and characterization of duck enteritis virus gi gene. | abstract: background: at present, alphaherpesviruses gi gene and its encoding protein have been extensively studied. it is likely that gi protein and its homolog play similar roles in virions direct cell-to-cell spread of alphaherpesviruses. but, little is known about the characteristics of dev gi gene. in this study, we expressed and presented the basic properties of the dev gi protein. results: the special 1221-bp fragment containing complete open reading frame(orf) of duck enteritis virus(dev ... | 2011 | 21595918 |