| molecular and immunological characterization of babesia gibsoni and babesia microti heat shock protein-70. | serological immunoscreening was used to identify a gene encoding heat shock protein-70 from babesia gibsoni (bghsp-70) that showed high homology with hsp-70s from other apicomplexan parasites. this gene corresponded to a full-length cdna containing an open reading frame of 1968 bp predicted to result in a 70-kda mature protein consisting of 656 amino acids. analysis of the expression levels of bghsp-70 indicated elevated transcription from cultured parasites incubated at 40 degrees c for 1 h, bu ... | 2009 | 19493212 |
| detection of a new pathogenic babesia microti-like species in dogs. | small babesiae in dogs are generally considered to belong to babesia gibsoni. here we describe the genotypic characterisation of small piroplasms found in the blood of a dog which suffered from clinical babesiosis. pairwise identities as well as distance, parsimony and maximum likelihood analyses of the 18s rdna clearly demonstrated that this isolate was only distantly related to the other canine piroplasms characterised genetically so far, including b. gibsoni. it was more closely related to b. ... | 2000 | 10760414 |
| babesiosis in washington state: a new species of babesia? | to characterize the etiologic agent (wa1) of the first reported case of babesiosis acquired in washington state. | 1993 | 8328736 |
| ribosomal dna probe for differentiation of babesia microti and b. gibsoni isolates. | the objective of this study was to determine whether different isolates of babesia microti could be distinguished from morphologically similar isolates of b. gibsoni by using a ribosomal dna (rdna) probe. a babesia-specific rdna probe was obtained by polymerase chain reaction amplification of sequences from b. microti dna using universal primers directed against highly conserved portions of the eukaryotic 16s-like rrna gene. the chemiluminescent rdna probe hybridized to southern blots of restric ... | 1992 | 1349901 |
| babesia--a historical overview. | the history of the genus babesia is briefly outlined. the classical differences with the main other genus of non-pigment-forming hemoparasites, theileria, are the absence of extra-erythrocytic multiplication (schizogony) in babesia and the cycle in the vector tick, which includes transovarial transmission in babesia but only transstadial transmission in theileria. also, the multiplication in the red cell of babesia, by budding, most often results in two daughter cells (merozoites), while that of ... | 2006 | 16513280 |
| babesia microti-like parasites detected in feral raccoons (procyon lotor) captured in hokkaido, japan. | raccoons (procyon lotor), which have recently become feral in japan, were examined for the presence of babesia microti-like parasites. out of 372 raccoons captured in the west-central part of hokkaido, 24 animals with splenomegaly were selected and tested by nested pcr targeting the babesial 18s rrna gene. b. microti-like parasites were detected in two of the 24 individuals, and their dna sequences were identical to that of the b. microti-like parasite reported from raccoons in the united states ... | 2005 | 16141672 |
| glucose uptake activity in murine red blood cells infected with babesia microti and babesia rodhaini. | the glucose uptake activity in babesia rodhaini and b. microti - infected red blood cell (irbc) was investigated in mice using 2-deoxy-d-glucose (2dog) and l-glucose (l-glc), a non-metabolizable analogue of d-glucose and non-incorporative glucose to non-infected rbc (nrbc), respectively. the uptake activities of both dog and l-glc were higher in irbcs than those in nrbc. the concentration dependent uptake of 2dog and l-glc in both irbc revealed a linear curve, indicating non-transporter mediated ... | 2004 | 15353845 |