| development and use of real-time pcr to detect and quantify mycoplasma haemocanis and "candidatus mycoplasma haematoparvum" in dogs. | two canine haemoplasma species have been recognised to date; mycoplasma haemocanis (mhc), which has been associated with anaemia in splenectomised or immunocompromised dogs, and "candidatus mycoplasma haematoparvum" (cmhp), recently described in an anaemic splenectomised dog undergoing chemotherapy. the study aim was to develop quantitative real-time pcr assays (qpcrs) incorporating an endogenous internal control to detect mhc and cmhp and to apply these assays to dna samples extracted from cani ... | 2010 | 19646827 |
| real-time pcr-based prevalence study, infection follow-up and molecular characterization of canine hemotropic mycoplasmas. | hemotropic mycoplasmas (hemoplasmas) have been reported in several mammalian species including dogs. infections may lead to hemolytic anemia, but investigations in the dog had been hampered by the lack of adequate diagnostic methods. only recently sensitive pcr-based assays were reported for the two canine hemoplasmas, mycoplasma haemocanis and candidatus mycoplasma haematoparvum. by applying these assays, 15.4% of 460 dogs from the south of france tested hemoplasma positive. it was hypothesized ... | 2008 | 17656047 |