| antibody response and virus tissue distribution in chickens inoculated with wild-type and recombinant fowl adenoviruses. | we demonstrated that the long tandemly repeated region (tr-2) is dispensable for in vitro replication of fowl adenovirus 9 (fadv-9). the tr-2-deleted recombinant fadv-9 expressing the enhanced green fluorescence protein was further characterized for in vivo effects. groups of chickens were exposed to recombinant or wild-type fadv-9 by intramuscular injection, through the feed or drinking water and one group served as a negative control. the antibody (ab) response, evaluated by elisa and a plaque ... | 2003 | 14604569 |
| a region at the left end of the fowl adenovirus 9 genome that is non-essential in vitro has consequences in vivo. | the regions at the left and right ends of fowl adenovirus (fadv) genomes are not well-characterized in comparison to those of human adenoviruses. using a series of deletion mutants, we analysed a 2.4 kb region near the left end of the fadv-9 genome (nt 400-2782) that contains packaging-signal motifs vi and vii and open reading frames (orfs) 0, 1, 1a, 1b, 1c and 2. viable viruses with specific deletions in this region had wild-type characteristics in vitro, as measured by cytopathic effect, plaqu ... | 2010 | 19759237 |
| the non-essential left end region of the fowl adenovirus 9 genome is suitable for foreign gene insertion/replacement. | the goals of this study were to demonstrate that a non-essential region at the left end of the fowl adenovirus 9 (fadv-9) genome could be used to generate recombinant viruses, examine their in vitro growth characteristics and determine their ability to transduce non-avian cells. three fadv-9 vectors (rfadv-9s) were generated carrying the enhanced-green fluorescent protein (egfp) gene: fadv-9inegfp, fadv-9 delta 1-egfp and fadv-9 delta 4-egfp. fadv-9inegfp carried the egfp cassette inserted into ... | 2010 | 20132849 |
| Antibody response and virus shedding of chickens inoculated with left end deleted fowl adenovirus 9-based recombinant viruses. | The nonpathogenic fowl adenoviruses (FAdVs) are suitable recombinant virus vectors. Two different replication-competent FAdV-9-based recombinant viruses carrying the enhanced green fluorescent protein (EGFP) gene within a nonessential DNA sequence at the left end genomic region were tested in chickens to study the antibody response by enzyme-linked immunosorbent assay to both the foreign proteins, EGFP and FAdV-9, and virus shedding through the feces. All inoculations were done intramuscularly: ... | 2011 | 22017044 |
| fowl adenovirus-based vaccine platform. | nonpathogenic fowl adenoviruses (fadvs) are amenable for engineering multivalent vaccine platforms due to large stretches of nonessential dna sequences in their genomes. we describe the generation of fadv-9-based vaccine platforms by targeted homologous recombination in an infectious clone (ppacfadv-9 or wild type fadmid) containing the entire viral genome in a cosmid vector. the viral dna is subsequently released from the cosmid by restriction enzyme digestion followed by transfection in a chic ... | 2017 | 28374242 |
| characterization and functional studies of fowl adenovirus 9 dutpase. | deoxyuridine 5'-triphosphate pyrophosphatase (dutpase), a ubiquitous enzyme that catalyzes the hydrolysis of dutp to dump and found in many viruses, has yet to be identified in fowl adenovirus 9 (fadv-9). by a multiple alignment of dutpase amino acid sequences, fadv-9 orf1 contained the five conserved motifs that define the protein family, and encoded a functional dutpase. moreover, transcription and protein expression patterns were characterized, indicating that dutpase was transcribed from 2h ... | 2016 | 27498408 |
| oral inoculation of chickens with a candidate fowl adenovirus 9 vector. | fowl adenoviruses (fadvs) are a potential alternative to human adenovirus-based vaccine vectors. our previous studies demonstrated that a 2.4-kb region at the left end of the fadv-9 genome is nonessential for virus replication and is suitable for the insertion or replacement of transgenes. our in vivo study showed that the virus fadv-9δ4, lacking six open reading frames (orfs) at the left end of its genome, replicates less efficiently than wild-type fadv-9 (wtfadv-9) in chickens that were infect ... | 2013 | 23740924 |
| foreign gene expression and induction of antibody response by recombinant fowl adenovirus-9-based vectors with exogenous promoters. | fowl adenoviruses (fadvs) are promising vectors for poultry vaccines and gene therapy. the commonly used human cytomegalovirus (cmv) promoter in recombinant fadv-9 viruses (recfadv-9s) leads to foreign gene expression that elicits an antibody response. despite its strength, studies have shown that the cmv promoter is prone to silencing by methylation hampering the in vivo application of vectors containing this promoter. therefore, to improve our virus vector system and circumvent potential limit ... | 2017 | 28780115 |