| determination of enzyme activities with the enzyme thermistor unit. | | 1979 | 109314 |
| pholipomycin, a new member of phosphoglycolipid antibiotics. iii. biological properties. | pholipomycin, a new member of the phosphoglycolipid antibiotics, was primarily active against gram-positive bacteria including clinically isolated resistant bacteria. it differed from other members of the antibiotic family as it also demonstrated activity against gram-negative bacteria. pholipomycin protected mice from infection with staphylococcus aureus and was nontoxic to mice having an ld50 (i.v.) of 600 mg/kg. besides possessing anti-microbiol activity, pholipomycin, when administered orall ... | 1977 | 413819 |
| [stability of the conformation of double spiral dna to gamma-irradiation]. | | 1975 | 813271 |
| avian glutamine phosphoribosylpyrophosphate amidotransferase propeptide processing and activity are dependent upon essential cysteine residues. | avian glutamine phosphoribosylpyrophosphate amidotransferase contains an nh2-terminal propetide-like sequence. nh2-terminal sequence analysis of immunoaffinity purified enzyme from chicken liver indicates that the propeptide is processed and the mature enzyme starts with cys. propeptide processing was investigated by site-directed mutagenesis using a system for expression in hela cells. glutamine-dependent activity and processing were abolished by replacement of the conserved cysteine at positio ... | 1992 | 1560022 |
| de novo purine nucleotide biosynthesis: cloning, sequencing and expression of a chicken purh cdna encoding 5-aminoimidazole-4-carboxamide-ribonucleotide transformylase-imp cyclohydrolase. | the purh cdna, encoding 5-aminoimidazole-4-carboxamide-ribonucleotide (aicar) transformylase-inosine monophosphate cyclohydrolase (atic), was cloned by functional complementation of an escherichia coli purh mutant using a chicken liver cdna expression library. this represents the first report of the cloning of any eukaryotic atic-encoding cdna (purh). the avian atic mrna is 2.3 kb long and encodes a protein with an mr of 64,422. the deduced amino acid sequence is 36% identical to the bacterial p ... | 1991 | 1937050 |
| cloning of a cdna encoding adenylosuccinate lyase by functional complementation in escherichia coli. | adenylosuccinate lyase was cloned by functional complementation of an escherichia coli purb mutant using an avian liver cdna expression library. the derived amino acid sequence is homologous to the bacterial purb-encoded adenylosuccinate lyase which catalyzes the same two steps in purine biosynthesis as the enzyme from animals. avian adenylosuccinate lyase also shows regions of extensive sequence similarity to the urea cycle enzyme, argininosuccinate lyase. this homology suggests a similar mecha ... | 1990 | 2111814 |
| cloning and expression of avian glutamine phosphoribosylpyrophosphate amidotransferase. conservation of a bacterial propeptide sequence supports a role for posttranslational processing. | the cdna for glutamine phosphoribosylpyrophosphate amidotransferase, the regulatory enzyme of de novo purine nucleotide biosynthesis, has been cloned for the first time from an animal. the derived amino acid sequence of the avian amidotransferase is homologous with amidotransferase sequences from bacteria and yeast. an 11-amino acid propeptide in bacillus subtilis amidotransferase is conserved in the avian enzyme. expression in chinese hamster ovary (cho) cells and escherichia coli provides evid ... | 1990 | 2123487 |
| the effect of bacillus natto on the t and b lymphocytes from spleens of feeding chickens. | the effect of bacillus natto on t and b lymphocytes in the chicken spleen was investigated. bacillus natto is isolated from fermented food and is used in japan in a preparation that enhances growth of farm animals. when chickens were fed 10(7) colony-forming units of b. natto (in spores) per gram of feed from hatching to 27 days of age there was an increase in the percentage of t and b lymphocytes in the spleens compared with non-b. natto-fed controls. the findings suggested that dietary bacillu ... | 1986 | 3488546 |
| a simple aerosol chamber for experimental reproduction of respiratory disease in pigs and other species. | the design of a simple aerosol chamber for experimental challenge of pigs and other species with respiratory pathogens is described. | 1985 | 3935301 |
| evolutionary stability of the histone genes of sea urchins. | | 1973 | 4200893 |
| separation of transfer rna's from bacillus subtilis, bacillus stearothermophilus and chicken liver by polyacrylamide gel electrophoresis. | | 1974 | 4209781 |
| ascaridia galli (nematoda): development and survival in gnotobiotic chickens. | | 1973 | 4632462 |
| microbiological laboratory hazard of bearded men. | an investigation was conducted to evaluate the hypothesis that a bearded man subjects his family and friends to risk of infection if his beard is contaminated by infectious microorganisms while he is working in a microbiological laboratory. bearded and unbearded men were tested with serratia marcescens and bacillus subtilis var. niger. contact aerosol transmission from a contaminated beard on a mannequin to a suitable host was evaluated with both newcastle disease virus and clostridium botulinum ... | 1967 | 4963447 |
| [d-penicillamine effects of penicillic-and penicillic acid in vivo]. | | 1967 | 4972064 |
| hexose-atp phosphotransferases: comparative aspects. ii. cross-reactivity of hexokinases from a variety of species with antisera to yeast hexokinase. | | 1971 | 5001183 |
| effect of dried bacillus subtilis culture on growth, body composition and hepatic lipogenic enzyme activity in female broiler chicks. | to investigate the effect of dried bacillus subtilis culture on growth, body composition and hepatic lipogenic enzyme activity, female broiler chicks were fed on either no additive (control) or dried b. subtilis-culture-supplemented commercial diets (215 g crude protein/kg, 12.85 mj metabolizable energy/kg) at 10 or 20 g/kg diet for 28 d from 14 to 42 d of age. body weight, and moisture, fat, protein and ash contents of the body were not influenced by the b. subtilis culture. feed efficiency, n ... | 1995 | 7577890 |
| control of de novo purine biosynthesis genes in ureide-producing legumes: induction of glutamine phosphoribosylpyrophosphate amidotransferase gene and characterization of its cdna from soybean and vigna. | soybean (glycine max) and mothbean (vigna aconitifolia) cdna clones encoding glutamine phosphoribosylpyrophosphate amidotransferase (prat), the first enzyme of the de novo purine biosynthesis pathway, have been isolated from nodule cdna libraries. the amino acid sequence deduced from soybean clone showed > 85% homology to the prat sequence of mothbean and 33-47% homology to those of bacteria, yeast, chicken, rat and human. the soybean clone encodes a protein with an n-terminal sequence resemblin ... | 1995 | 7894513 |
| two genes for de novo purine nucleotide synthesis on human chromosome 4 are closely linked and divergently transcribed. | a cdna encoding human glutamine phosphoribosylpyrophosphate amidotransferase for step one in de novo purine nucleotide synthesis was cloned, sequenced, and expressed in chinese hamster ovary cells to yield functional enzyme. enzyme function was dependent upon removal of an 11-amino-acid propeptide. a mutant enzyme having three propeptide amino acid replacements was not processed and was not active. the human genes gpat, encoding the amidotransferase, and airc, encoding a bifunctional enzyme for ... | 1994 | 8106516 |
| determination of pyridonecarboxylic acids in plasma by reverse-phase high-performance liquid chromatography. | a simple qualitative and quantitative determination for pyridonecarboxylic acids including nalidixic acid (na), oxolinic acid (oa) and pipemidic acid (ppa) in chicken plasma was carried out by microbiological, spectrophotometric, thin-layer chromatographic (tlc) and reverse-phase high-performance liquid chromatographic (hplc) methods. as a test organism for bacteriological bioassay, bacillus subtilis atcc-6633 was the most sensitive of seven organisms investigated. using the cup and the disc met ... | 1994 | 8170407 |
| molecular cloning of rat amidophosphoribosyltransferase. | the cdna of rat amidophosphoribosyltransferase (ec 2.4.2.14, atase), which is the supposed regulatory allosteric enzyme of de novo purine nucleotide biosynthesis, has been cloned by polymerase chain reaction. the predicted open reading frame encodes a protein of 517 amino acids with a deduced molecular weight of 57,436 including a supposed 11-amino acid propeptide. the 16 amino acid residues next to the propeptide were identical to the n-terminal amino acid microsequence of a purified rat liver ... | 1993 | 8463258 |
| stunting syndrome in broilers: effect of age and exogenous amylase and protease on performance, development of the digestive tract, digestive enzyme activity, and apparent digestibility. | day-old male, meat-type chicks raised in brooder batteries were infected by orally administering an inoculum prepared from intestines of broiler chicks infected with stunting syndrome (ss). naive controls were kept in a parallel room. the chicks were fed a commercial starter diet supplemented with two levels of enzyme preparations to 14 d of age. the experiment was continued to the age of 6 wk in order to estimate compensatory feed intake and growth. in a parallel study, digestibility of the fee ... | 1995 | 8825593 |
| pharmacokinetics of enrofloxacin given by the oral, intravenous and intramuscular routes in broiler chickens. | enrofloxacin was given to broiler chickens, 3 groups of 6 birds each, at a dose of 5 mg/kg. routes of administration were intravenous (i.v.), intramuscular (i.m.) and oral (p.o.) and blood samples were collected from the jugular vein for determination of serum drug levels over a 54-hour period after administration. drug levels were determined using bacillus subtilis spore suspension on meuller-hinton antibiotic medium. intravenous administration produced drug levels which followed a bi-exponenti ... | 1999 | 10480461 |
| [experimental evaluation of the biological safety of gene-engineered bacteria using a model strain bacillus subtilis interferon-producing strain]. | the in vitro and in vivo evaluation of the biological and ecological safety of genetically modified bacteria (gmb) was carried out on b. subtilis recombinant strain 2335/105, capable of producing human interferon alpha-2, used as experimental model. as shown in this investigation made with the use of bacteriological analysis and polymerase chain reaction, the oral administration of gmb to calves, chickens and white mice produced no disturbances in the microbial ecology of the gastrointestinal tr ... | 2001 | 11548250 |
| evaluation and establishing the performance of different screening tests for tetracycline residues in animal tissues. | four methods intended for screening muscle tissue for residues belonging to the tetracycline group were compared using artificially contaminated as well as incurred samples. two agar diffusion methods were studied: one with bacillus subtilis as a test strain, the second with bacillus cereus. two variants of each method were compared: thin plates for analysis of intact or minced meat, and thick plates for analysis of meat fluid. the thin plate variants could not be evaluated with artificially con ... | 2004 | 14754636 |
| effects of a dried bacillus subtilis culture on egg quality. | the effects of a dried bacillus subtilis culture on the egg qualities of layers were studied. nine hundred and sixty 25-wk-old lohmann brown layers were randomly divided into 5 groups with 192 layers in each group. layers in group 1 were fed a control diet. the remaining groups received the control diet that contained either 20 mg of zinc bacitracin/kg and 4 mg of colistin sulfate/kg or 500, 1,000, or 1,500 mg of b. subtilis culture/ kg, respectively. the results showed improvements in egg produ ... | 2006 | 16523640 |
| recombinant eggshell ovocalyxin-32: expression, purification and biological activity of the glutathione s-transferase fusion protein. | the avian eggshell is a highly ordered biomineral composed mainly of calcium carbonate associated with an organic matrix composed of proteins, glycoproteins and proteoglycans. this structure provides the developing embryo with protection from physical damage and microbial invasion. ovocalyxin-32 (ocx-32) is a 32 kda eggshell-specific matrix protein which has been cloned and demonstrates 30% identity with the mammalian carboxypeptidase inhibitor, latexin. in order to further study its function, r ... | 2007 | 17344082 |
| hide depilation and feather disintegration studies with keratinolytic serine protease from a novel bacillus subtilis isolate. | keratinases play an important role in biotechnological applications such as improvement of feather meal, enzymatic dehairing and production of amino acids or peptides from high molecular weight substrates. bacillus subtilis p13, isolated from vajreshwari hot spring (45-50 degrees c) near mumbai, india, produces a neutral serine protease and has an optimum temperature of 65 degrees c. this enzyme preparation was keratinolytic in nature and could disintegrate whole chicken feathers, except for the ... | 2008 | 18264704 |
| comparative antibacterial activity of avian egg white protein extracts. | 1. egg white proteins from the eggs of domestic chicken (gallus gallus), turkey (meleagris gallopavo), duck (anas platyrhynchos) and goose (anser anser) were analysed in order to compare the antimicrobial activity of these products. 2. albumen from each species was sampled and analysed by sds-page and western blotting. antimicrobial activity and lysozyme activity were measured. 3. ovotransferrin and ovalbumin were identified in all species while c-type lysozyme was present in chicken, turkey and ... | 2008 | 18409086 |
| determination of biotin (vitamin h) by the high-performance affinity chromatography with a trypsin-treated avidin-bound column. | a method for measuring biotin by affinity-chromatography was developed using a trypsin-treated avidin silica gel column. elution was by a linear gradient of propan-2-ol in an acidic phosphate buffer system containing 0.7 m nacl (ph 2.4). biotin was derivatized with 9-anthryldiazomethane (adam) to the fluorescent biotin-adam ester and a linear calibration line was obtained from 0 to 1.39 pmol with a detection limit of 69.5 fmol. biotin was measured after hydrolysis in 2.25 m sulphuric acid for 1h ... | 2008 | 18514598 |
| cloned bacillus subtilis alkaline protease (apra) gene showing high level of keratinolytic activity. | the bacillus subtilis alkaline protease(apra) gene was previously cloned on a pubho-derivative plasmid. high levels of expression and gene stability were demonstrated when b. subtilis cells were grown on the laboratory medium 2xsg. b. subtilis cells harboring the multicopy apra gene were grown on basal medium, supplemented with 1 % chicken feather as a source of energy, carbon, and nitrogen. proteolytic and keratinolytic activities were monitored throughout the cultivation time. a high level of ... | 1998 | 18575989 |
| [purification and characterization of fibrinolytic enzyme from bacillus subtilis bs-26]. | thrombolytic therapy is a safe and effective treatment for thrombotic diseases. microorganisms are possible sources of thrombolytic drugs. we purified and characterized fibrinolytic enzyme produced by bacillus subtilis strain bs-26. | 2008 | 19160823 |
| hybrid antimicrobial enzyme and silver nanoparticle coatings for medical instruments. | we report a method for the synthesis of antimicrobial coatings on medical instruments that combines the bacteriolytic activity of lysozyme and the biocidal properties of silver nanoparticles. colloidal suspensions of lysozyme and silver nanoparticles were electrophoretically deposited onto the surface of stainless steel surgical blades and needles. electrodeposited films firmly adhered to stainless steel surfaces even after extensive washing and retained the hydrolytic properties of lysozyme. th ... | 2009 | 20355960 |
| characterization of a multifunctional feather-degrading bacillus subtilis isolated from forest soil. | in this study, we isolated and characterized a novel feather-degrading bacterium that shows keratinolytic, antifungal and plant growth-promoting activities. a bacterium s8 was isolated from forest soil and confirmed to belong to bacillus subtilis by biolog system and 16s rrna gene analysis. the improved culture conditions for the production of keratinolytic protease were 0.1% (w/v) sorbitol, 0.3% (w/v) kno(3), 0.1% (w/v) k(2)hpo(4), 0.06% (w/v) kh(2)po(4) and 0.04% (w/v) mgcl(2)·6h(2)o (ph 8.0 a ... | 2010 | 20454836 |
| effect of bacillus-based direct-fed microbials on eimeria maxima infection in broiler chickens. | the effect of dietary bacillus-based direct-fed microbials (dfms; eight single strains designated as bs2084, lssao1, 3ap4, bs18, 15ap4, 22cp1, bs27, and bs278, and one multiple-strain dfm product [avicorr]) on growth performance, intestinal lesions, and innate and acquired immunities were evaluated in broiler chickens following eimeria maxima (em) infection. em-induced reduction of body weight gain and intestinal lesions were significantly decreased by addition of 15ap4 or bs27 into broiler diet ... | 2010 | 20621358 |