| pulsed-field gel electrophoresis for sub-specific differentiation of serpulina pilosicoli (formerly 'anguillina coli'). | pulsed-field gel electrophoresis (pfge) was developed for subspecific differentiation of serpulina pilosicoli, and was applied to 52 isolates recovered from cases of intestinal spirochaetosis (is) in pigs, dogs, human beings and various avian species. the technique was highly sensitive, differentiating the isolates into 40 groupings. only six groups contained more than one isolate; in five of these groups isolates with the same banding pattern were either from pigs in the same herds (four groups ... | 1996 | 8764512 |
| light microscopic and ultrastructural changes in the ceca of chicks inoculated with human and canine serpulina pilosicoli. | light microscopic and ultrastructural changes were observed in chicks challenged with north american serpulina pilosicoli, a weakly beta-hemolytic intestinal spirochete (wbhis) associated with human and canine intestinal spirochetosis. chicks in control groups received trypticase soy broth or canine serpulina innocens. the birds were necropsied at weekly intervals, and the ceca were processed for bacteriologic and pathologic examinations. no wbhis were isolated from the ceca of chicks in the con ... | 1996 | 8885181 |
| the prevalence of serpulina pilosicoli in humans and domestic animals in the eastern highlands of papua new guinea. | in a survey of five villages in the eastern highlands of papua new guinea, serpulina pilosicoli was isolated from rectal swabs from 113 of 496 individuals (22.8%). colonization rates ranged from 22.6-30.1% in four of the villages but was only 8.6% in the other village. in comparison colonization was demonstrated in only 5 of 54 indigenous people (9.3%) and none of 76 non-indigenous people living in an urban environment in the same region. colonization did not relate to reported occurrence of dia ... | 1997 | 9440442 |
| canine intestinal spirochetes consist of serpulina pilosicoli and a newly identified group provisionally designated "serpulina canis" sp. nov. | the spirochetes inhabiting the large intestines of humans and animals consist of a diverse group of related organisms. intestinal spirochetosis caused by serpulina pilosicoli is a newly recognized enteric disease of human beings and animals with potential public health significance. the purpose of this study was to determine the species identity of canine intestinal spirochetes by comparing 30 isolates obtained from dogs in australia (n = 25) and the united states (n = 5) with reference strains ... | 1998 | 9666003 |
| population genetic analysis of serpulina pilosicoli and its molecular epidemiology in villages in the eastern highlands of papua new guinea. | the population genetics of serpulina pilosicoli and its molecular epidemiology in villages in the eastern highlands province of papua new guinea were investigated. multilocus enzyme electrophoresis (mlee) was used to analyse 164 isolates from humans and animals. these were divided into 33 electrophoretic types (ets), four of which contained 65% of the isolates. the mean genetic diversity (n = number of ets) for 145 human isolates was 0.18, and the mean number of alleles at five polymorphic loci ... | 1998 | 9734020 |
| brachyspira pilosicoli isolated from two beagles and one mongrel in japan. | the feces, colonic contents, and colonic mucosa from two beagles and a mongrel with and without mucohemorrhagic diarrhea were anaerobically cultured and after 72 hr, lucent and rough colonies with weak beta-hemolysis were observed. small spirochetes with one or two loose waves in the colonies were observed under a phase contrast microscope and the spirochete cells sometimes aggregated. the 16srdna sequencing results demonstrated that the canine isolates were b. pilosicoli and the base alignments ... | 2004 | 15187377 |
| prevalence of brachyspira pilosicoli and "brachyspira canis" in dogs and their association with diarrhoea. | the aims of this study were to investigate the prevalence of colonization with intestinal spirochaetes in dogs, and to assess their association with diarrhoea. to achieve this, faecal samples from 311 dogs were obtained between november 2008 and april 2009 and cultured for brachyspira species. a total of 41 brachyspira spp. isolates were recovered, and these were classified into species according to their biochemical properties, and results of a b. pilosicoli species-specific pcr, and partial am ... | 2010 | 20570060 |
| faecal excretion of intestinal spirochaetes by urban dogs, and their pathogenicity in a chick model of intestinal spirochaetosis. | this study aimed to obtain information about the types of spirochaetes colonising urban dogs in thailand, and to investigate their pathogenic potential in a day-old chick model of intestinal spirochaetosis. spirochaetes were isolated from the faeces of six of 47 (12.8%) healthy dogs and 11 of 104 (10.6%) dogs with diarrhoea. their biochemical properties and 16s ribosomal dna sequences were analysed. four isolates were identified as brachyspira pilosicoli, three resembled "brachyspira pulli", nin ... | 2011 | 21320714 |