| cloning and expression of the pasteurella multocida toxin gene, toxa, in escherichia coli. | a chromosomal dna library of a toxigenic type d strain of pasteurella multocida subsp. multocida was established in escherichia coli. from this library two clones, spe308 and spe312, were identified by using a monoclonal antibody against the osteoclast-stimulating p. multocida toxin (pmt). extracts of these clones showed cytopathic activity identical to that of extracts of toxigenic p. multocida. the recombinant plasmids, pspe308 and pspe312, directed the synthesis of a protein with an apparent ... | 1989 | 2680987 |
| microorganisms associated with a pneumonic epizootic in rocky mountain bighorn sheep (ovis canadensis canadensis). | a comprehensive study of a pneumonic epizootic was initiated when the first signs of disease were noted in a metapopulation of bighorn sheep inhabiting hells canyon, bordering idaho, oregon, and washington. a total of 92 bighorn sheep were tested for etiologic agents during the following 6-mo study period. the study population included bighorn sheep believed to be the subpopulation in which disease was first noted, and these sheep were translocated to a holding facility in an effort to contain t ... | 2007 | 18229860 |
| specificity of dna probes for the detection of toxigenic pasteurella multocida subsp. multocida strains. | five dna probes directed against different regions of the gene that encodes the dermonecrotic toxin of pasteurella multocida subsp. multocida were examined for their ability to identify toxigenic p. multocida subsp. multocida strains. the specificities of the probes were studied with 96 strains of p. multocida subsp. multocida and 22 strains of 11 other bacterial species. results of colony hybridization assays using these probes indicated that two of the five probes have potential diagnostic val ... | 1990 | 2203816 |
| detection of tetracycline-resistant and susceptible pasteurellaceae in the nasopharynx of loose group-housed calves. | the aim of the present study was to determine which pasteurella and mannheimia species are present in the upper respiratory tract of healthy calves with no history of antimicrobial treatment prior to sampling. the presence of subpopulations of tetracycline-resistant pasteurellaceae was also investigated. nasal swabs from 61 loose group-housed, clinically healthy calves, 1 to 4 months old, from 16 dairy herds were inoculated aerobically on a selective medium (columbia agar with 5% ovine blood and ... | 2006 | 17004035 |
| in vitro efficacy of n-duopropenide, a recently developed disinfectant containing quaternary ammonium compounds, against selected gram-positive and gram-negative organisms. | to study the efficacy of n-duopropenide against various gram-positive and gram-negative organisms. | 1999 | 10211693 |
| sialic acid metabolism and systemic pasteurellosis. | pasteurella multocida subsp. multocida is a commensal and opportunistic pathogen of food animals, wildlife, and pets and a zoonotic cause of human infection arising from contacts with these animals. here, an investigation of multiple serotype a strains demonstrated the occurrence of membrane sialyltransferase. although p. multocida lacks the genes for the two earliest steps in de novo sialic acid synthesis, adding sialic acid to the growth medium resulted in uptake, activation, and subsequent tr ... | 2005 | 15731025 |