detection of simian t-lymphotropic virus type i using the polymerase chain reaction. | to develop the polymerase chain reaction (pcr) for the detection of simian t-lymphotropic virus type i (stlv-i) infection, cell lines or peripheral-blood mononuclear cells (pbmc) from 2 non-human primate species [african green monkeys (agm), cercopithecus aethiops; baboon, papio cynocephalus] were evaluated for their stlv-i status using oligonucleotide primer pairs and probes specific for the tax and pol gene regions of the closely related human t-lymphotropic virus type i (htlv-i). these pcr re ... | 1992 | 1312066 |
simian t-lymphotropic virus type 1 (stlv-1) infection in wild yellow baboons (papio hamadryas cynocephalus) from mikumi national park, tanzania. | serum and peripheral blood leukocytes from wild yellow baboons (papio hamadryas cynocephalus) were tested for the presence of stlv-1-specific antibodies and proviral dna. fourteen of 30 sera tested positive by radioimmunoprecipitation assay (ripa) with htlv-1. among 36 dna samples tested by pcr 15 were positive by double nested pcr for a fragment of the stlv-1 env gene, the most sensitive assay among pcr tests employed. of 30 animals that were tested both serologically and by pcr in only 1 case ... | 1997 | 9123842 |
the three human t-lymphotropic virus type i subtypes arose from three geographically distinct simian reservoirs. | to investigate the origin of human t-lymphotropic virus i (htlv-i), strains of diverse geographical origin were analysed. we sequenced the ltr and env genes of htlv-i strains from brazil, central african republic, taiwan and zaire, and the simian t-lymphotropic virus type i (stlv-i) strain phsu1 from a baboon from the sukhumi primate centre. we performed phylogenetic analyses using neighbour-joining, parsimony and maximum likelihood methods. three separate htlv-i clusters were identified intersp ... | 1996 | 8627240 |
genetic analysis and molecular phylogeny of simian t-cell lymphotropic virus type i: evidence for independent virus evolution in asia and africa. | type c retroviruses, designated simian t-cell lymphotropic virus type i (stlv-i), have been isolated from several genera of old world monkeys and apes, but not from new world monkeys and prosimians. to determine the genomic diversity and molecular evolution of stlv-i and to clarify their genetic relationship to human t-cell lymphotropic virus type i (htlv-i), we enzymatically amplified, then directly sequenced selected regions of the gag, pol, env, and px genes of stlv-i strains from asia and af ... | 1994 | 8116255 |
immune recognition of genetically diverse simian t-cell lymphotropic virus type i isolates. | nucleotide sequence analysis of selected regions of the gag, pol, env and px genes of simian t-cell lymphotropic virus type i (stlv-i) strains indicated that african isolates were more closely related to human t-cell lymphotropic virus type i (htlv-i) than asian isolates. despite these recent comparative studies on nucleotide sequence homology between htlv-i and stlv-i isolates, only limited information is available regarding the influence of genetic differences on antigen-antibody recognition o ... | 1995 | 7535998 |