| serum complement-like opsonic activities in human, animal, vegetable, and proprietary milks. | human, animal, proprietary, and soy milks are comparable to human serum c5 in opsonization of baker's yeast. bovine milk and human serum opsonically reconstitute c5-deficient mouse serum. such reconstitution is selectively inhibited by antiserum to human c5. further characterization suggests that bovine milk contains material structurally and functionally similar, but not identical, to human c5. | 1977 | 323975 |
| glucan induced modification of experimental staphylococcus aureus infection in normal, leukemic and immunosuppressed mice. | glucan, a beta 1 leads to 3 polyglucosidic component of saccharomyces cerevisiae, was evaluated for its ability to provide nonspecific resistance to s. aureus septicemia in akr/j mice. intravenous injection of glucan (0.45 mg/mouse) 7 and 4 days prior to intravenous challenge with s. aureus (1.0 x 10(9)) resulted in a significantly increased survival as compared to control mice. histological examination of the kidneys revealed that glucan decreased tissue necrosis associated with systemic staphy ... | 1979 | 547728 |
| the primary structure of the major cytoplasmic valine trna of mouse myeloma cells. | this paper describes the derivation of the primary structure of the major valine trna in the cytoplasm of mouse myeloma cells. approximately 75% of the nucleotide sequence of this trna is also shared by the trna1-val of yeast, this homology serving as a further indication of the extreme conservation of the structures of the trnas of different eukaryotic organisms. a novel feature of mouse myeloma trna1-val is its loop iv sequence: -u-psi-c-g-m1a-a-a-. this particular loop iv sequence has not pre ... | 1975 | 1091487 |
| genetic activity spectra of some antischistosomal compounds, with particular emphasis on thioxanthenones and benzothiopyranoindazoles. | in this review we note that hycanthone (etrenol) is mutagenic for bacteriophage, bacteria, yeast, neurospora, drosophila, and for mammalian tissue culture cells, and we point out other genetic activities of this thioxanthenone and of related compounds. one alarming genetic activity is the ability of hycanthone to cause transformation of tissue culture cells in vitro in a test designed to detect carcinogens, results that parallel the direct demonstration of carcinogenic activity of hycanthone in ... | 1975 | 1107580 |
| competitive elisa for serodiagnosis of bluetongue: evaluation of group-specific monoclonal antibodies and expressed vp7 antigen. | the performance of 2 competitive enzyme-linked immunosorbent assays (c-elisa) was compared with the reference c-elisa i for the detection of antibodies to bluetongue virus (btv). one of the assays (c-elisa ii) used a group-specific monoclonal antibody (mab) to btv, obtained from the american type culture collection (8a3b-6) and tissue culture (tc)-derived btv antigen (ag), and the other assay (c-elisa iii) used btv core protein vp7 (expressed in yeast) and the reference mab (pirbright laboratory ... | 1992 | 1325189 |
| the vph1 gene encodes a 95-kda integral membrane polypeptide required for in vivo assembly and activity of the yeast vacuolar h(+)-atpase. | yeast vacuolar acidification-defective (vph) mutants were identified using the ph-sensitive fluorescence of 6-carboxyfluorescein diacetate (preston, r. a., murphy, r. f., and jones, e. w. (1989) proc. natl. acad. sci. u.s.a. 86, 7027-7031). vacuoles purified from yeast bearing the vph1-1 mutation had no detectable bafilomycin-sensitive atpase activity or atp-dependent proton pumping. the peripherally bound nucleotide-binding subunits of the vacuolar h(+)-atpase (60 and 69 kda) were no longer ass ... | 1992 | 1385813 |
| the role of the cathepsin d propeptide in sorting to the lysosome. | the propeptides of lysosomal enzymes have been implicated in membrane association and mannose 6-phosphate-independent sorting to the lysosome (rijnboutt, s., aerts, h., geuze, h. j., tager, j. m., and strous, g. j. (1991) j. biol. chem. 266, 4862-4868; mcintyre, g. f., and erickson, a. h. (1991) j. biol. chem. 266, 15438-15445). in this report, the function of the propeptide of procathepsin d in sorting to the lysosome was directly assessed using a cathepsin d deletion mutant lacking the propept ... | 1992 | 1400484 |
| secretion of alpha-hemolysin by bovine mammary isolates of staphylococcus aureus. | a total of 262 strains of staphylococcus aureus isolated from the mammary gland of dairy cows were examined for the production of alpha-hemolysin. strains were cultured in a liquid medium of casein hydrolysate and yeast extract in an atmosphere of 7% (v/v) co2 in air. the assay consisted of a dot immunoblotting technique employing bacterial culture supernatants and a mouse monoclonal antibody specific for alpha-hemolysin. ninety-four percent (247) of 262 strains were positive for alpha-hemolysin ... | 1992 | 1423065 |
| nuclear and mitochondrial inheritance in yeast depends on novel cytoplasmic structures defined by the mdm1 protein. | the mdml mutation causes temperature-sensitive growth and defective transfer of nuclei and mitochondria into developing buds of yeast cells at the nonpermissive temperature. the mdm1 gene was cloned by complementation, and its sequence revealed an open reading frame encoding a potential protein product of 51.5 kd. this protein displays amino acid sequence similarities to hamster vimentin and mouse epidermal keratin. gene disruption demonstrated that mdm1 is essential for mitotic growth. antibodi ... | 1992 | 1378448 |
| identification of the fourth member of the mammalian endoprotease family homologous to the yeast kex2 protease. its testis-specific expression. | we used the polymerase chain reaction to identify a mouse testis cdna that represented another member of a growing class of mammalian endoproteases involved in the processing of precursor proteins. this cdna encoded a 655-residue protein, designated pc4, containing a bacterial subtilisin-like catalytic domain closely related to those of the recently characterized precursor-processing endoproteases, furin, pc1/pc3, pc2, and kex2. within this domain, the amino acid sequence of pc4 was 70, 58, 55, ... | 1992 | 1372895 |
| a mouse cdc25-like product enhances the formation of the active gtp complex of human ras p21 and saccharomyces cerevisiae ras2 proteins. | gdp-dissociation stimulators (gdss) are the key element for the regeneration of the active state of ras proteins, but despite intensive investigations, little is so far known about their functional and structural properties, particularly in mammals. a growing number of genes from various organisms have been postulated to encode gdss on the basis of sequence similarity with the saccharomyces cerevisiae cdc25 gene, whose product acts as a gds of ras proteins. however, except for cdc25 and the rela ... | 1992 | 1447167 |
| integration of heterologous genes into the chromosome of saccharomyces cerevisiae using a delta sequence of yeast retrotransposon ty. | distribution of a delta (delta) sequence of the ty element on a chromosome of the yeast saccharomyces cerevisiae was analysed by pulsed-field gel electrophoresis. more than 100 copies of the delta sequence were nonrandomly distributed on the chromosome. using the delta sequence as a recombination site, mouse alpha-amylase and human beta-endorphin genes were introduced into the chromosomal dna. the integration occurred on a particular chromosome in each case and the copy number was estimated as t ... | 1990 | 1369269 |
| evaluation of foreign gene codon optimization in yeast: expression of a mouse ig kappa chain. | we have optimized the codons in an immunoglobulin kappa chain gene to those preferred in the yeast saccharomyces cerevisiae. the mutant and wild type kappa chain genes were each fused with a synthetic invertase signal peptide that also contained only yeast-preferred codons, and expressed in the f762 yeast strain. the use of yeast-preferred codons resulted in a more than 5-fold increase in the rate of synthesis and at least a 50-fold increase in the steady state level of protein. | 1991 | 1367771 |
| functional complementation of yeast ste6 by a mammalian multidrug resistance mdr gene. | multidrug resistance in mammalian tumor cells is associated with the overexpression of mdr genes encoding p-glycoproteins, which function as drug efflux pumps. a yeast homolog of mdr, ste6, mediates export of a-factor mating peptide. yeast mata cells carrying a ste6 deletion produce no extracellular a-factor and therefore are defective in mating. expression of a complementary dna for the mouse mdr3 gene in a yeast ste6 deletion strain restored ability to export a-factor and to mate. a mutation ( ... | 1992 | 1348873 |
| okadaic acid and p13suc1 modulate the reinitiation of meiosis in mouse oocytes. | short-term exposure to okadaic acid (oa), a specific inhibitor of protein phosphatases 1 and 2a, induced resumption of meiosis, including metaphase spindle formation, in mouse oocytes treated with a phosphodiesterase inhibitor, while long incubations with oa arrested oocyte maturation at a step prior to spindle formation. to explore the basis for this difference, the overall patterns of protein synthesis and phosphorylation and the production of tissue-type plasminogen activator (tpa), the synth ... | 1992 | 1333241 |
| a mouse cdc25 homolog is differentially and developmentally expressed. | the timing and activation of the p34cdc2 kinase in mammals is associated with dephosphorylation of phosphotyrosine and phosphothreonine residues on the p34cdc2 kinase. for fission yeast, the timing of mitosis is regulated by cyclic accumulation of cdc25, which promotes dephosphorylation of p34cdc2 and concomitant protein kinase activation. we report the identification and characterization of a structural and functional mouse homolog, cdc25m2, of the cdc25 phosphatase. cdc25m2 shows high sequence ... | 1992 | 1313771 |
| mouse mammary tumor gene int-3: a member of the notch gene family transforms mammary epithelial cells. | expression of a 2.3-kb rna species is induced in mammary tumors as a consequence of insertional mutagenesis at the int-3 locus by the mouse mammary tumor virus. the nucleotide sequence and biological activity of this mammary tumor-specific int-3 rna species were determined. it contains an open reading frame which encodes a 57-kda protein. the translated protein possesses six nearly contiguous 32-amino-acid repeats which are related to a similar motif in the saccharomyces cerevisiae cdc-10-encode ... | 1992 | 1312643 |
| the ubiquitous transactivator zfp-38 is upregulated during spermatogenesis with differential transcription. | we describe the complete nucleotide sequence of a full length cdna clone encoding a new mouse zinc finger protein gene, zfp-38 and localize it on chromosome 5 by the interspecific backcross analysis. the n-terminal domain of the zfp-38 protein (64 kda) contains 358 amino acids and the c-terminal domain of 197 residues encodes 7 zinc fingers. we also present evidence that zfp-38 is a strong transcriptional activator. the transactivation domain was localized in the non finger region and a fusion p ... | 1992 | 1284028 |
| chemistry and pharmacology of naproxen. | the need for a nonsteroidal anti-inflammatory agent effective in rheumatoid arthritis, osteoarthritis, gout, ankylosing spondylitis and related diseases with reduced side effects when compared to existing drugs led us to develop naproxen: d-2-(6'-methoxy-2'-naphthyl)-propionic acid. this new agent is a highly effective anti-inflammatory, analgetic, and antipyretic agent in the rodent administered orally. in a rat paw edema test for anti-inflammatory activity naproxen was 55 times more active tha ... | 1975 | 1173774 |
| [newer antimycotics. i. derivatives of phenyl-hydrazine (author's transl)]. | the author produced a number of derivatives of phenyl-hydrazine and its analogues, investigated their antimicrobial efficiency in vitro, and discussed the association of chemical structure with the fungistatic effect in the series of phenyl-hydrazine-derivatives. from the author's results it can be concluded that the halogen-substituted phenyl-hydrazines and their n-(acetyl)-derivatives exert a moderate fungistatic effect (tab. 1). in the series of n-aryl-sulphonyl-n'-phenyl-hydrazines only the ... | 1975 | 1099848 |
| [experimental candida vaginitis in the mouse (author's transl)]. | manifest candida albicans infection of the vagina of mice was achieved only after oestrogen treatment. histologically, different phases of vaginal candidiasis could be observed: the mycotic infection began with a massive colonization of candida albicans in the horny layers of the vaginal epithelium and an early immigration of granulocytes. abscess-like changes followed when high number of yeast cells were used for infection. one to two weeks after onset the inflammatory changes decreased and fun ... | 1976 | 1036880 |
| severe generalized glutathione reductase deficiency after antitumor chemotherapy with bcnu" [1,3-bis(chloroethyl)-1-nitrosourea]. | patients receiving bcnu [1,3-bis(2 chloroethyl)-1-nitrosourea] acquire a profound deficiency of erythrocytic oxidized glutathione reductase (gssg-r) within minutes after the first intravenous injection of a single therapeutic dose (75 mg/m.2) of the drug. this effect is not accompanied by changes in the activites of 19 additional erythrocytic enzymes tested, is reproducible in vitro in a dose-related manner, and is not caused by the antitumor agents administered concurrently with the nitrosourea ... | 1977 | 870569 |
| classification of polyene antibiotics according to chemical structure and biological effects. | fourteen polyene antibiotics and six of their semisynthetic derivatives were compared for their effects on potassium (k(+)) leakage and lethality or hemolysis of either saccharomyces cerevisiae or mouse erythrocytes. these polyene antibiotics fell into two groups. group i antibiotics caused k(+) leakage and cell death or hemolysis at the same concentrations of added polyene. in this group fungistatic and fungicidal levels were indistinguishable. group i drugs included one triene (trienin); tetra ... | 1979 | 393163 |
| the intrasanguineous host-mediated assay procedure distribution and retention of yeast in the mouse. | a study of the factors that could affect a method to detect mutations in cells recovered from different organs after intravenous injection in mice, was performed by using the d4 strain of saccharomyces cerevisiae. the recovery of the yeast cells 5 min to 3 days after injection in the host animal was investigated. the circulation, distribution and localization of the cells were determined, and histopathologic analysis was performed in order to detect possible interactions between the mice and the ... | 1979 | 390379 |
| complement and fc receptor-mediated phagocytosis of normal and stimulated mouse peritoneal macrophages. | | 1979 | 385329 |
| mutagenicity of industrial compounds. vii. styrene and styrene oxide: ii. point mutations, chromosome aberrations and dna repair induction analyses. | the possible genetic effects produced by styrene have been investigated by means of different methodologies in several biological organisms: (a) the induction of point mutation has been investigated in salmonella typhimurium (reverse mutation), in the yeast schizosaccharomyces pombe (forward mutation), both in vitro and in vivo, in the host-mediated assay of mice, and in the chinese hamster cell line grown in vitro (v-79) (forward mutation); (b) the induction of chromosome mutation has been inve ... | 1978 | 366743 |
| cryptococcus neoformans: pseudohyphal forms surviving culture with acanthamoeba polyphaga. | during experiments on the gastrointestinal tract as a possible portal of entry for cryptococcus neoformans, we occasionally observed the free-living amoeba, acanthamoeba polyphaga, growing in the presence of c. neoformans cultured from mouse feces. examination of the amoebic trophozoites revealed that they were engorged with yeast cells. over a period of 2 to 3 weeks of incubation, the amoebae apparently killed most of the yeast cells. some of the surviving c. neoformans cells formed atypical co ... | 1978 | 352931 |
| [factors influencing formation of dinitrosyl complexes of non-heme iron in vitro preparations of mouse liver and yeasts]. | total content of fenh and its amounts incorporated into paramagnetic dinitrozyl complexes of fenh (complexes 2.03) which are formed in vitro in homogenates of mouse liver and yeast preparations treated with nitrogen oxide was determined by means of chemical and esr methods. formation of the complexes 2.03 in the liver homogenate is limited by the content of easily dyalized weakly bound fenh, in yeasts--by the content of pair rs-groups. it is suggested that in the liver preparation fenh incorpora ... | 1977 | 198016 |
| transfer of trnas to somatic cells mediated by sendai-virus-induced fusion. | trnas from yeast (trnaphe and 4s rna) and escherichia coli (suiii+ trnaityr) have been transferred to mouse cells by means of a two-step transfer procedure [loyter, zakai, and kulka (1975) j. cell biol. 66, 292-304; schlegel and rechsteiner (1975) cell 5, 371-379]. in the first stage of the transfer trnas were incorporated into rabbit red blood cells (rbcs). thereafter, the loaded erythrocytes were fused with recipient mouse cells by means of sendai virus. at least 0.3-0.4% of the total input of ... | 1976 | 183211 |
| the distribution of pyrophosphatidic acid in nature. | the occurrence of a novel phospholipid, pyrophosphatidic acid, in the lipid extracts of yeasts (23 species), bacteria (e. coli), algae (chlorella), mammalia (human, rabbit, guinea pig, and mouse), insect (cockroach), fish (carp), mollusc (clam), and spermatophyta (spinach) was investigated. pyrophosphatidic acid was found exclusively in the lipid extracts of several kinds of yeast species, but not in other normal living species (animals, plants, and microorganisms) so far investigated. all of th ... | 1975 | 175047 |
| [autoradiography of the exchanges that can occur between murine sarcoma cells (bp 8) and yeast cells (saccharomyces cerevisiae, complete or protoplast)]. | we can tell after observing the resulting negative that yeasts are able, in our experimental conditions, to collect a sequence of adn and of arn proceeding from the cancerous cells. then, those yeasts could turn their synthesis towards the production of new materials. if those informed yeasts were introduced into a mouse, they would induce at the level of the immunocompetent cells a specific immunizing antitumoral power. | 1977 | 145297 |
| the selective isolation of novel cdnas encoded by the regions surrounding the human interleukin 4 and 5 genes. | we have developed modifications to direct cdna selection that allow the rapid and reproducible isolation of low abundance cdnas encoded by large genomic clones. biotinylated, cloned genomic dnas are hybridized in solution with amplifiable cdnas. the genomic clones and attached cdnas are captured on streptavidin coated magnetic beads, the cdnas are eluted and amplified. we have applied this protocol to a 425kb yac that contains the human il4 and il5 genes. after two cycles of enrichment twenty-fo ... | 1992 | 1408833 |
| development of immunogenic recombinant oka varicella vaccine expressing hepatitis b virus surface antigen. | recombinant oka varicella vaccine expressing hepatitis b virus (hbv) surface antigen (hbs) was constructed by inserting the hbs gene into the viral thymidine kinase (tk) gene and was examined for its immunogenicity in guinea-pigs. the hbs gene encoding 25 amino acids of pres2 and the whole of the s region was inserted into the tk gene of the cloned plasmid. the chimeric plasmid dna and oka varicella vaccine dna were cotransfected and recombinant virus was isolated after immunofluorescence screen ... | 1991 | 1646279 |
| [interaction of mrna of the p53 protein gene with ribosomal rna]. | by blot hybridization we found that dna fragments of eukaryotic 18 and 28s rrnas bind specifically with mrna. in these experiments the in vitro transcribed mrna of mouse gene p53 was used. in addition we found that both 18 and 28s rrnas were able to form intermolecular complexes with mrnas of several genes 18s rrna-mrna; 28s rrna-mrna, but fail to bind the antisense rnas of the same genes. the experimental data allow to suppose that 18s and 28s rrnas carry several fragments that are complementar ... | 1992 | 1435781 |
| transmission blocking antibody of the plasmodium falciparum zygote/ookinete surface protein pfs25 also influences sporozoite development. | the plasmodium falciparum zygote/ookinete surface protein, pfs25, persists in the oocyst wall throughout its development. anti-25 kd transmission blocking antibody, given to infected anopheles stephensi or a. gambiae mosquitoes in an additional bloodmeal, 3-6 days after being fed gametocyte infected blood, penetrated the oocyst and reacted with the 25 kd protein within it. this reaction caused a significant reduction in the number of developing sporozoites. mouse serum containing antibodies rais ... | 1992 | 1437237 |
| th1 and th2 cytokine secretion patterns in murine candidiasis: association of th1 responses with acquired resistance. | two chemically mutagenized agerminative variants of candida albicans were used to immunize mice against challenge with highly virulent cells of the parent strain. although both mutants (vir- 3 and vir- 13) resulted in nonlethal infection and could be recovered from mouse organs for many days after the intravenous inoculation of 10(7) to 10(6) cells, significant protection to systemic challenge with virulent c. albicans was induced by only one (vir- 3) of the two variants. anticandidal resistance ... | 1991 | 1682265 |
| transcription and replication of animal mitochondrial dnas. | the development of in vitro transcription and replication systems has allowed the identification of promoter sequences and origins of replication for several animal mtdnas. as a consequence, the necessary reagents and basic information are available to permit the characterization of transacting factors that are required for transcription and replication. all of the animal trans-acting species purified at this time are known or reasoned to be nuclear gene products. there is now the opportunity to ... | 1992 | 1452432 |
| molecular cloning and structural analysis of genes from zea mays (l.) coding for members of the ras-related ypt gene family. | we have isolated, cloned, and characterized two cdnas from zea mays (l.), denoted yptm1 and yptm2, encoding proteins related to the ypt protein family. amino acid similarity scores with ypt1 from yeast and ypt from mouse are in the range of 70% for yptm1 and 74% for yptm2, respectively, whereas similarities with p21 ras and other ras-related proteins are less than 40%. most amino acid residues showing identity are clustered in the gtp/gdp binding domain. in addition, two cysteine residues close ... | 1992 | 1731354 |
| red blood cells as an antigen-delivery system. | the use of adjuvants is usually required to induce strong immunological responses to protein antigens. however, in many cases these adjuvants cannot be extensively applied in human and veterinary vaccinations because of associated inflammatory reactions or granuloma formation. we show here that protein antigens (bovine serum albumin, hog liver uricase, and yeast hexokinase), coupled to autologous red blood cells by way of a biotin-avidin-biotin bridge, elicit an immunological response in mice si ... | 1992 | 1457052 |
| targeting sequences of the two major peroxisomal proteins in the methylotrophic yeast hansenula polymorpha. | dihydroxyacetone synthase (das) and methanol oxidase (mox) are the major enzyme constituents of the peroxisomal matrix in the methylotrophic yeast hansenula polymorpha when grown on methanol as a sole carbon source. in order to characterize their topogenic signals the localization of truncated polypeptides and hybrid proteins was analysed in transformed yeast cells by subcellular fractionation and electron microscopy. the c-terminal part of das, when fused to the bacterial beta-lactamase or mous ... | 1992 | 1465101 |
| effect of oral saccharomyces boulardii treatment on the activity of clostridium difficile toxins in mouse digestive tract. | human antibiotic-associated diarrhoea and pseudomembranous colitis are partly due to toxin production by clostridium difficile. it is now well documented that saccharomyces boulardii protects against c. difficile induced diseases. in an attempt to understand better the mechanism of this protective effect, the action of s. boulardii on a crude toxin preparation was studied in vitro and in vivo. the results showed that the yeast had no effect on the toxins in vitro but was able to protect mice ino ... | 1992 | 1488767 |
| the mouse enhancer trap locus 1 (etl-1): a novel mammalian gene related to drosophila and yeast transcriptional regulator genes. | a novel mouse gene, enhancer trap locus 1 (etl-1), was identified in close proximity to a lacz enhancer trap integration in the mouse genome showing a specific beta-galactosidase staining pattern during development. in situ analysis revealed a widespread but not ubiquitous expression of etl-1 throughout development with particularly high levels in the central nervous system and epithelial cells. the amino acid sequence of the etl-1 protein deduced from the cdna shows strong similarity, over a st ... | 1992 | 1489724 |
| evidence for a conserved 95-120 kda subunit associated with and essential for activity of v-atpases. | vacuoles purified from saccharomyces cerevisiae bearing the vph1-1 mutation had no detectable bafilomycin-sensitive atpase activity or atp-dependent proton pumping. furthermore, the vacuolar h(+)-atpase (v-atpase) nucleotide binding subunits were no longer associated with vacuolar membranes yet were present at wild-type levels in yeast whole-cell extracts. the vph1 gene was cloned by screening a lambda gt11 expression library with antibodies directed against a 95 kda vacuolar integral membrane p ... | 1992 | 1491220 |
| recombinant repair of diverged dnas: a study of homoeologous chromosomes and mammalian yacs in yeast. | recombinational repair is the means by which dna double-strand breaks (dsbs) are repaired in yeast. dna divergence between chromosomes was shown previously to inhibit repair in diploid g1 cells, resulting in chromosome loss at low nonlethal doses of ionizing radiation. furthermore, 15-20% divergence prevents meiotic recombination between individual pairs of saccharomyces cerevisiae and s. carlsbergensis chromosomes in an otherwise s. cerevisiae background. based on analysis of the efficiency of ... | 1992 | 1495486 |
| yeast transcription factor iid participates in cell-free transcription of a mammalian ribosomal protein tata-less promoter. | we analysed transcription of the gene for the ribosomal protein (rp) l32 of the mouse, which is transcribed in mouse l1210 nuclear extracts in vitro. the rpl32 gene lacks a canonical tata box. hence it has been suggested that this gene has an alternative transcription pathway not requiring transcription factor iid (tfiid). selective inactivation of tfiid in nuclear extract completely abolished the transcription of rpl32 in vitro. selective inactivation was restored by the addition of cloned and ... | 1992 | 1497610 |
| identification of the antibiotic hops component, colupulone, as an inducer of hepatic cytochrome p-4503a in the mouse. | a higher level of cytochrome p-450 (p450)-dependent ethylmorphine (em) n-demethylase activity was observed in hepatic microsomes from mice fed a natural-ingredient diet ("crude diet") than in those from mice fed a semi-purified diet ("purified diet"). this led to the testing of individual ingredients of the crude diet as inducers of the p-450 system. brewers yeast proved to be the most significant inductive component of the crude diet. further investigation revealed that hop components (lupulone ... | 1992 | 1352202 |
| yeast site-specific ribonucleoprotein endoribonuclease mrp contains an rna component homologous to mammalian rnase mrp rna and essential for cell viability. | rnase mrp is a site-specific ribonucleoprotein endoribonuclease that cleaves rna sequence complementary to mammalian mitochondrial origins of replication in a manner consistent with a role in primer rna metabolism. the same activity in the yeast saccharomyces cerevisiae has recently been identified; it cleaves an rna substrate complementary to a yeast mitochondrial origin of replication at an exact site of linkage of rna to dna. we have purified this yeast enzyme further and detect a single, nov ... | 1992 | 1398074 |
| strategies for advancement of short-term mutagenicity tests: on the optimal ionic strength for the liver microsomal assay. | the aim of this work was to optimize the ionic strength (tau) in the liver microsomal assay (lma) in performing short-term genotoxicity tests. tau optimization would increase the sensitivity (i.e. decrease false negatives) and at the same time increase the specificity (decrease false positives). such optimization depends upon the relative activities and stabilities of the liver polysubstrate cytochrome p450- and fad-containing monooxygenase-dependent metabolizing enzymes present in the incubatio ... | 1992 | 1499090 |
| identification of an estrogen response element in the 3'-flanking region of the murine c-fos protooncogene. | we have used transient transfection assays with reporter plasmids expressing chloramphenicol acetyltransferase, linked to regions of mouse c-fos, to identify a specific estrogen response element (ere) in this protooncogene. this element is located in the untranslated 3'-flanking region of the c-fos gene, 5 kilobases (kb) downstream from the c-fos promoter and 1.5 kb downstream of the poly(a) signal. this element confers estrogen responsiveness to chloramphenicol acetyltransferase reporters linke ... | 1992 | 1517237 |
| effect of lectins on hepatic clearance and killing of candida albicans by the isolated perfused mouse liver. | the isolated perfused mouse liver model was used to study the effects of various lectins on hepatic trapping and killing of candida albicans. after mouse livers were washed with 20 to 30 ml of perfusion buffer, 10(6) c. albicans cfu were infused into the livers. at the time of recovery, 63% +/- 2% (mean +/- standard error of the mean) of the infused c. albicans cfu were recovered from the liver and 14% +/- 1% were recovered from the effluent for a total recovery of 77% +/- 2%. this indicated tha ... | 1992 | 1541519 |
| chromosomal localization of a human cdna containing a dids binding domain and demonstrating high homology to yeast omnipotent suppressor 45. | we recently have identified a full-length cdna (tb3-1) from a human adenocarcinoma cell line t84 cdna library that encodes a 47.8-kda protein. tb3-1 shares identity with the putative yeast translation termination factor omnipotent suppressor 45. using human-mouse somatic cell panel analysis, a family of sequences with high homology to the tb3-1 cdna clone were localized to human chromosomes 5, 6, 7, and x. southern analysis of a panel of mammalian and chicken genomic dna demonstrates that tb3-1 ... | 1992 | 1546371 |
| ethyl carbamate metabolism: in vivo inhibitors and in vitro enzymatic systems. | the metabolism of ethyl carbamate and the localization of its metabolites have been shown to be almost completely inhibited by ethanol in the mouse [waddell, marlowe, pierce: food chem. toxicol.25, 527 (1987); yamamoto, pierce, hurst, chen, waddell: drug metab. dispos. 16, 355 (1988)]. the enzyme system catalyzing this metabolism which is inhibited by ethanol now has been further investigated in both in vivo and in vitro studies. there is a direct, highly significant relationship between the ext ... | 1990 | 1974186 |
| properties of the nuclear p1 protein, a mammalian homologue of the yeast mcm3 replication protein. | polyclonal antibodies were raised against a multiprotein 'holoenzyme' form of calf thymus dna polymerase alpha-primase and used to probe a human cdna-protein expression library constructed in the lambda gt11 vector. the probe identified a series of cdna clones derived from a 3.2 kb mrna which encodes a novel 105 kda polypeptide, the p1 protein. in intact cells, the p1 protein was specifically associated with the nucleus, and in cell extracts, it was associated with complex forms of dna polymeras ... | 1992 | 1549468 |
| molecular cloning and characterization of interferon alpha/beta response element binding factors of the murine (2'-5')oligoadenylate synthetase me-12 gene. | seven clones encoding interferon response element binding factors have been isolated from a mouse fibroblast lambda gt11 cdna library by using a 32p end-labeled tandem trimer of the mouse (2'-5')oligoadenylate synthetase gene interferon response element as a probe. clone 16 shares strong similarity (95%) at both dna and amino acid level with yb-1, a human major histocompatibility complex class ii y-box dna-binding protein, and with dbpb, a human epidermal growth factor receptor gene enhancer reg ... | 1991 | 1986360 |
| evidence that candida albicans binds via a unique adhesion system on phagocytic cells in the marginal zone of the mouse spleen. | we recently demonstrated by using an ex vivo adhesion assay that candida albicans yeast cells exhibit a unique binding affinity for the marginal zone of the spleen. this binding event provides a working model for studying mechanisms of organ dissemination of the fungus from the blood. by using the ex vivo assays reported here, we showed by bright-field and electron microscopic techniques that mouse spleen marginal zone cells capable of ingesting india ink particles are also involved in yeast cel ... | 1992 | 1563789 |
| gene products that promote mrna turnover in saccharomyces cerevisiae. | we showed previously that the increased rate of mrna turnover associated with premature translational termination in the yeast saccharomyces cerevisiae requires a functional upf1 gene product. in this study, we show that the upf1 gene codes for a 109-kda primary translation product whose function is not essential for growth. the protein contains a potential zinc-dependent nucleic acid-binding domain and a nucleoside triphosphate-binding domain. a 300-amino-acid segment of the upf1 protein is 36% ... | 1992 | 1569946 |
| molecular cloning and expression of hsp82 gene of the dimorphic pathogenic fungus histoplasma capsulatum. | we have cloned a nucleotide sequence from histoplasma capsulatum g222b corresponding to a heat inducible hsp82 gene, and determined its entire sequence and the flanking regions. during the temperature-controlled mycelium-to-yeast phase transition the gene is more actively transcribed at 37 degrees c in the temperature tolerant and mouse-virulent g222b strain, while 34 degrees c is the optimum for transcription in the temperature sensitive and mouse-avirulent downs strain. | 1992 | 1581351 |
| co-operativity of functional domains in the muscle-specific transcription factor myf-5. | myf-5 is a member of a family of muscle-specific transcription factors that activate myogenesis in 10t1/2 fibroblasts. here we report the analysis of myf-5 structural domains that are responsible for its biological activity. site-directed mutagenesis revealed that two clusters of basic amino acids within a conserved basic region and two amphipathic helices within the adjacent hlh domain are essential for sequence-specific dna binding and hetero-oligomerization, respectively. transcriptional acti ... | 1992 | 1582413 |
| elevated expression of the ribosomal protein s2 gene in human tumors. | differential screening of a cdna library was used to isolate genes differentially expressed in a nontumorigenic clone and a ras-transformed variant of the human teratocarcinoma cell line pa-1. the rna transcript for one of the cdna clones that we identified was expressed at a 25-fold higher level in the ras-transformed pa-1 cells than in the nontumorigenic pa-1 cells. dna sequence analysis of this clone showed that it had 86% nucleic acid homology to the mouse llrep3 gene and only differed at a ... | 1992 | 1586449 |
| gastrointestinal candidiasis in a murine model of severe combined immunodeficiency syndrome. | a murine model of severe combined immunodeficiency syndrome (scid mice) affords an opportunity to study the interaction of candida albicans with a host lacking functional b- and t-cell mechanisms. we have previously reported no significant difference in yeast recovery after intravenous challenge of balb/c mice and scid mice with c. albicans (s. mahanty, r.a. greenfield, w.a. joyce, and p.w. kincade, infect. immun. 56:3162-3166, 1988). in this study, we evaluate the course of gastrointestinal can ... | 1991 | 2037373 |
| cloning and characterization of transcripts showing virulence-related gene expression in naegleria fowleri. | the pathogenic lee strain of naegleria fowleri isolated from human or mouse brain loses pathogenicity when cultured axenically in a nutrient broth. to identify genes differentially expressed in highly virulent versus weakly virulent amoebae, a cdna library was constructed by using mrna from amoebae recovered from a mouse brain. two cdna clones were isolated by differential screening of the library. the transcript homologous to clone nf314 was preferentially expressed in highly virulent cells, wh ... | 1992 | 1587609 |
| identification of the cbp1 polypeptide in mitochondrial extracts from saccharomyces cerevisiae. | cbp1 is a nuclearly encoded yeast protein required for stability of mitochondrial cytochrome b pre-mrna. previous studies have shown that cbp1 stabilizes the cytochrome b transcripts via interaction with the 5'-end. for the present study, both rabbit polyclonal and mouse monoclonal antibodies against cbp1 were prepared using a trpe-cbp1 fusion polypeptide as a source of antigen. cbp1 was undetectable in a crude mitochondrial fraction from a wild-type strain by western blot assay, but a 66-kda im ... | 1990 | 2104848 |
| kex2-like endoproteases pc2 and pc3 accurately cleave a model prohormone in mammalian cells: evidence for a common core of neuroendocrine processing enzymes. | two mammalian gene products, pc2 and pc3, have been proposed as candidate neuroendocrine-precursor processing enzymes based on the structural similarity of their catalytic domains to that of the yeast precursor-processing endoprotease kex2. in this report we demonstrate that these two proteases can cleave proopiomelanocortin (pomc) in the secretory pathway of mammalian cells. similarly to pituitary corticotrophs, pc3 expressed in processing-deficient bsc-40 cells cleaved native mouse pomc at the ... | 1991 | 1647029 |
| interferon-inducible mouse mx1 protein that confers resistance to influenza virus is gtpase. | the murine mx1 protein is an interferon-inducible nuclear protein and confers resistance to influenza virus infection even though the resistance mechanism is yet unclear. the mx1 protein contains a tripartite gtp-binding domain consisting of gxxxxgks, dxxg, and t/nkxd motifs. in the gtpase gene superfamily such as p21ras protein, signal-transducing g protein, and translation elongation factor, the gtpase activity plays a key role in each protein function. here we show that gtpase activity is ind ... | 1991 | 1657964 |
| new yeast actin-like gene required late in the cell cycle. | actin, a major cytoskeletal component of all eukaryotic cells, is one of the most highly conserved proteins. it is involved in various cellular processes such as motility, cytoplasmic streaming, chromosome segregation and cytokinesis. the actin from the yeast saccharomyces cerevisiae, encoded by the essential act1 gene, is 89% identical to mouse cytoplasmic actin and is involved in the organization and polarized growth of the cell surface. we report here the characterization of act2, a previousl ... | 1992 | 1729653 |
| mouse pulmonary cytochrome p-450 naphthalene hydroxylase: cdna cloning, sequence, and expression in saccharomyces cerevisiae. | we have isolated a cdna clone, nah-2, encoding the cytochrome p-450nah (naphthalene hydroxylase) from a mouse lung lambda zap cdna library using anti-cytochrome p-450nah igg as a probe. this same antibody selectively blocked [nagata, k., martin, b.m., gillette, j.r., & sasame, h.a. (1990) drug metab. dispos. 18, 557-564] the cytochrome p-450 in mouse lung microsomes that catalyzed the conversion of naphthalene to (1r,2s)-naphthalene 1,2-oxide, which has been postulated as a causative agent in th ... | 1991 | 1742282 |
| the hydrophilic and acidic n-terminus of the integral membrane enzyme phosphatidylserine synthase is required for efficient membrane insertion. | the product of the yeast cho 1 gene, phosphatidylserine synthase (pss), is an integral membrane protein that catalyses a central step in cellular phospholipid biosynthesis. a 1.2 kb fragment containing the regulatory and structural components of the cho 1 gene was sequenced. transcription initiation in wild-type cells was found to occur between -1 and -15 relative to the first atg of a large open reading frame capable of encoding a 30,804 molecular weight protein. this translation initiation sit ... | 1990 | 2168611 |
| transfer of yeast artificial chromosomes from yeast to mammalian cells. | human dna can be cloned as yeast artificial chromosomes (yacs), each of which contains several hundred kilobases of human dna. this dna can be manipulated in the yeast host using homologous recombination and yeast selectable markers. in relatively few steps it is possible to make virtually any change in the cloned human dna from single base pair changes to deletions and insertions. in order to study the function of the cloned dna and the effects of the changes made in the yeast, the human dna mu ... | 1991 | 1755830 |
| proton-linked sugar transport systems in bacteria. | the cell membranes of various bacteria contain proton-linked transport systems for d-xylose, l-arabinose, d-galactose, d-glucose, l-rhamnose, l-fucose, lactose, and melibiose. the melibiose transporter of e. coli is linked to both na+ and h+ translocation. the substrate and inhibitor specificities of the monosaccharide transporters are described. by locating, cloning, and sequencing the genes encoding the sugar/h+ transporters in e. coli, the primary sequences of the transport proteins have been ... | 1990 | 2172229 |
| recognition of the cdei motif gtcacatg by mouse nuclear proteins and interference with the early development of the mouse embryo. | we have reported previously (1) two unexpected consequences of the microinjection into fertilized mouse eggs of a recombinant plasmid designated p12b1, carrying a 343 bp insert of non-repetitive mouse dna. injected at very low concentrations, this plasmid could be established as an extrachromosomal genetic element. when injected in greater concentration, an early arrest of embryonic development resulted. in the present work, we have studied this toxic effect in more detail by microinjecting shor ... | 1991 | 1766880 |
| prohormone-converting enzymes: regulation and evaluation of function using antisense rna. | several putative peptide-processing endoproteases have been identified by homology to the yeast kex2 endoprotease, including furin, pc2, and pc1. however, the question is still open as to which might be involved in peptide posttranslational processing. to enable detailed comparisons of physiological changes in peptide processing with biochemical and molecular biological studies, we cloned rat pituitary cdnas for pc1 and pc2. the amino acid sequence homologies among rat, human, and mouse pc1, pc2 ... | 1991 | 1791845 |
| the use of historical data for identifying biologically unimportant but statistically significant results in genotoxicity assays. | the definition of a negative result is a problem in genetic toxicology. here we suggest that a result may be considered biologically unimportant (negative) if it falls within the limits of variation usually found in the negative controls of the particular test. to determine 'usual' variation, we have set 95% confidence limits on three indices of variation, calculated from historical values for duplicate negative control data from several genotoxicity tests. these tests showed four characteristic ... | 1990 | 2188068 |
| role of endogenously derived leukotrienes in the regulation of lysosomal enzyme expression in macrophages exposed to beta 1,3-glucan. | the expression of the lysosomal enzyme hexosaminidase has been shown to be stimulated by the exposure of mouse macrophages to beta 1,3-glucan, a particulate component of yeast cell walls and zymosan particles. exposure of mouse peritoneal macrophages to particulate beta 1,3-glucan (100 micrograms/ml) was also found to stimulate the production of eicosanoids from both the cyclooxygenase (prostaglandin e2) and 5'-lipoxygenase (leukotriene c4) pathways. the objective of this study was to determine ... | 1991 | 1825502 |
| furin: the prototype mammalian subtilisin-like proprotein-processing enzyme. endoproteolytic cleavage at paired basic residues of proproteins of the eukaryotic secretory pathway. | furin, the translational product of the recently discovered fur gene, appears to be the first known mammalian member of the subtilisin family of serine proteases and the first known mammalian proprotein-processing enzyme with cleavage selectivity for paired basic amino acid residues. structurally and functionally, it resembles the prohormone-processing enzyme, kexin (ec 3.4.21.61), which is encoded by the kex2 gene of yeast saccharomyces cerevisiae. most likely, furin is primarily involved in th ... | 1991 | 1843280 |
| binary system for regulating transgene expression in mice: targeting int-2 gene expression with yeast gal4/uas control elements. | we have developed a binary transgenic system that activates an otherwise silent transgene in the progeny of a simple genetic cross. the system consists of two types of transgenic mouse strains, targets and transactivators. a target strain bears a transgene controlled by yeast regulatory sequences (uas) that respond only to the yeast transcriptional activator gal4. a transactivator strain expresses an active gal4 gene that can be driven by any selected promoter. the current paradigm uses the muri ... | 1991 | 1846961 |
| drosophila rna polymerase ii elongation factor dms-ii has homology to mouse s-ii and sequence similarity to yeast ppr2. | dmsii is a drosophila rna polymerase ii elongation factor which suppresses pausing by rna polymerase ii at specific sites on double stranded templates. using antibodies produced against the purified protein, a drosophila cdna expression library was screened and a cdna was isolated which encoded a portion of dmsii. when this cdna was used to probe kc cell mrna the predominant species was found to be 1.4 kb in length. the original cdna was used to screen a drosophila kc cell cdna library resulting ... | 1990 | 2243775 |
| activation of polyomavirus dna replication by yeast gal4 is dependent on its transcriptional activation domains. | the polyomavirus replication origin contains transcriptional regulatory sequences. to determine how these elements function in dna replication, and to learn whether a common mechanism underlies the activation of transcription and dna replication, we tested whether a well-characterized transcriptional activator, yeast gal4, was capable of stimulating dna replication and transcription in the same mammalian cell line. we observed that gal4 activated polyomavirus dna replication in mouse cells when ... | 1991 | 1849079 |
| cell-cycle aspects of growth and maturation of mammalian oocytes. | in this review, recent data concerning growth and maturation of nonmammalian and mammalian female germ cells are compiled with regard to the increased understanding of somatic cells mitotic cycles, from yeast to human tissues. these data allow us to conclude that growing oocytes of nonvertebrates, lower vertebrates, and mammals resemble somatic cells in the g1 phase of the mitotic cycle in their metabolic and cell cycle behavior. transcriptional and translational activity of growing oocytes and ... | 1990 | 2264998 |
| coupling of protein synthesis and mitochondrial import in a homologous yeast in vitro system. | we made use of a homologous cell-free mitochondrial protein import system derived from the yeast saccharomyces cerevisiae to investigate the coupling of protein synthesis and import. mitochondrial precursor proteins were synthesized in a yeast lysate either in the presence or absence of isolated yeast mitochondria. we were, therefore, able to analyze protein import into mitochondria either in a strictly posttranslational reaction (when isolated mitochondria were added only after protein synthesi ... | 1991 | 1849892 |
| the yeast homolog to mouse tcp-1 affects microtubule-mediated processes. | a saccharomyces cerevisiae homolog to drosophila melanogaster and mouse tcp-1 encoding tailless complex polypeptide 1 (tcp1) has been identified, sequenced, and mapped. the mouse t complex has been under scrutiny for six decades because of its effects on embryogenesis and sperm differentiation and function. tcp1 is an essential gene in yeast cells and is located on chromosome 4r, linked to pet14. the tcp1-encoded proteins in yeast, drosophila, and mouse cells share between 61 and 72% amino acid ... | 1991 | 1901944 |
| transcriptional activation domain of the muscle-specific gene-regulatory protein myf5. | the human muscle determination factor myf5, like myod and other members of the family of skeletal muscle-specific regulatory proteins, contains a highly conserved putative helix-loop-helix domain. in myod this motif is required for the initiation of myogenesis in c3h mouse 10t1/2 fibroblasts and other non-muscle cells as well as for transcriptional activation of muscle genes. high affinity dna binding of myod to regulatory dna elements in muscle genes requires the formation of heterodimers with ... | 1990 | 2385294 |
| ty element transposition: reverse transcriptase and virus-like particles. | we have found reverse transcriptase activity and virus-like particles only in yeast cells that contain a galactose-promoted ty element induced on galactose. the cofractionation of reverse transcriptase, genomic-length ty rna, and a ty-specified protein antigen in a particulate fraction and the ability of this complex to synthesize specifically a product that is homologous to the entire ty suggest that reverse transcription of ty rna takes place in the particle. the absence of appreciable levels ... | 1985 | 2411424 |
| a genomic clone encoding a novel proliferation-dependent histone h2a.1 mrna enriched in the poly(a)+ fraction. | replication-dependent histone mrnas are prime examples of nonpolyadenylated mrnas. we isolated and characterized cdnas and a genomic clone for a replication-dependent histone h2a.1 mrna which segregated into the poly(a)+ fraction during mrna isolation through an oligo(dt)-cellulose column. however, the results of sequencing of the genomic clone suggested that the mrna did not contain a poly(a) tail. instead, the genomic sequence revealed a nonterminal oligo(a) tract directly upstream from the ty ... | 1990 | 1971418 |
| nucleotide sequence of mouse hsp60 (chaperonin, groel homolog) cdna. | the cdna sequence of the 60 kda heat-shock protein from mouse 3t3 cells has been determined. the deduced amino acid sequence of mouse hsp60 protein differs from the corresponding proteins from chinese hamster and human cells in 7 and 13 residues, respectively, most of which are conservative replacements. | 1990 | 1979012 |
| expression and inducibility of drug-metabolizing enzymes in novel murine liver epithelial cell lines and their ability to activate procarcinogens. | four novel nontransformed epithelial cell lines, isolated from fetal or adult mouse liver, were tested: (a) to determine the profile of xenobiotic metabolizing enzymes; (b) to evaluate the inducibility of the polysubstrate (cytochrome p-450-dependent) monooxygenase system by various classes of inducers; and (c) to assess the capacity of the cells to metabolize structurally different procarcinogens. with regard to the phase i pathway, the cells expressed various p-450 (class ia, ia2, iib, iie1, i ... | 1991 | 1988092 |
| identification of a cdna encoding a second putative prohormone convertase related to pc2 in att20 cells and islets of langerhans. | pc2 and furin are two recently identified members of a class of mammalian proteins homologous to the yeast precursor processing protease kex2 and the bacterial subtillisins. we have used the polymerase chain reaction to identify and clone a cdna (pc3) from the mouse att20 anterior pituitary cell line that represents an additional member of this growing family of mammalian proteases. pc3 encodes a 753-residue protein that begins with a signal peptide and contains a 292-residue domain closely rela ... | 1991 | 1988934 |
| properties and applications of human dna repair genes. | the importance of understanding dna repair processes is discussed in terms of the origins of human cancer. several human repair genes have been mapped to specific human chromosomes using somatic cell hybrids. it is noteworthy that 3 of these genes lie in the same region of chromosome 19: genes ercc1 and ercc2, which are involved in nucleotide excision repair, and xrcc1, which is involved in the repair of strand breaks. the genes xrcc1 and ercc2 were cloned from cosmid libraries prepared from dna ... | 1991 | 2011139 |
| mammalian camp-dependent protein kinase functionally replaces its homolog in yeast. | the cdna encoding the catalytic subunit (c alpha) from mouse camp-dependent protein kinase (pk) was expressed in saccharomyces cerevisiae. by a plasmid swap procedure, we demonstrated that the mammalian c alpha subunit can functionally replace its yeast homolog to maintain the viability of a yeast strain containing genetic disruptions of the three tpk genes encoding the yeast c subunits. c alpha subunit produced in yeast was purified and its biochemical properties were determined. the protein is ... | 1991 | 2022331 |
| the human hprt gene on a yeast artificial chromosome is functional when transferred to mouse cells by cell fusion. | a 680-kb yeast artificial chromosome (yac) that contains a functional copy of the human hypoxanthine phosphoribosyltransferase (hprt) gene has been isolated. this yac, yhprt, and another yac, yxy837, which contains the 3' end of the hprt gene, have been mapped with restriction enzymes that cleave human dna infrequently. the hprt gene lies near the center of yhprt. fusion of yhprt-containing yeast spheroplasts with mouse l a-9 cells, which are hprt-negative, gives rise to hprt-positive colonies. ... | 1991 | 2037299 |
| molecular mechanisms involved in the regulation of gene expression during cell differentiation and development. | one general point to emerge from this review is that each step in the pathway for gene expression from dna to final polypeptide via chromosome structure, primary transcript, mrna processing, stability, and translation is exploited in some developmental context as a means of regulation. moreover, there is little evidence that the regulation of different stages in this pathway is associated consistently with particular classes of genes or developmental processes: for example, homeotic and other re ... | 1990 | 2090260 |
| the c terminus of the nf-kappa b p50 precursor and an i kappa b isoform contain transcription activation domains. | the p50 subunit of the nf-kappa b transcription complex is derived from the n-terminal half of a larger precursor protein, p105. although a fair amount is known about functions located within the p50 sequences, less is known about the c-terminal half of p105. in this report, we have identified a potent transcription activation domain located in the c terminus of mouse p105. in addition, the i kappa b beta proteins chicken p40 and human mad-3, proteins that are related to the p105 c terminus, str ... | 1992 | 1598203 |
| the mouse homolog to the ras-related yeast gene ypt1 maps on chromosome 11 close to the wobbler (wr) locus. | | 1992 | 1643310 |
| the yeast gal4 protein transactivates the polyomavirus origin of dna replication in mouse cells. | we have replaced the polyomavirus (py) enhancer, which is an essential component of the py origin of dna replication (ori), with five repeats of a 17-bp oligonucleotide including the yeast gal4 upstream activating sequence (5xgal4 sites). plasmids containing this modified py ori, designated test plasmids, and plasmids encoding either the gal4 transcriptional activator protein or various derivatives of this protein were cotransfected into mouse cells which constitutively synthesize a temperature- ... | 1991 | 1645781 |
| purification and properties of mouse liver coproporphyrinogen oxidase. | coproporphyrinogen oxidase was purified to homogeneity from mouse liver. the specific activity of the pure enzyme was 3500 nmol.h-1.mg-1; its apparent molecular mass (35 kda) was confirmed by immunological characterization of the enzyme in a trichloroacetic-acid-precipitated total-liver-protein extract. the native enzyme appeared to be a dimer of 70 kda as determined by gel filtration under nondenaturating conditions. the km value for coproporphyrinogen iii was 0.3 microm. the purified enzyme wa ... | 1989 | 2540974 |
| the thiol proteinase inhibitors improve the abnormal rapid down-regulation of protein kinase c and the impaired natural killer cell activity in (chediak-higashi syndrome) beige mouse. | protein kinase c (pkc) is essential in intracellular signal transduction for various cell functions including natural killer (nk) cell activity. this enzyme is hydrolysed by calpain, which is ca2+-dependent thiol proteinase. we showed here that in nk activity-deficient beige (bg/bg) mouse, the model of chediak-higashi syndrome, the translocated membrane-bound pkc activity declined rapidly in nk cell-enriched lymphocytes after tpa stimulation. however, the rapid decline was abolished by the pretr ... | 1989 | 2541700 |
| phototumorigenesis studies of 5-methoxypsoralen in bergamot oil: evaluation and modification of risk of human use in an albino mouse skin model. | the skin of the female hairless albino mouse (skh 1) was used to study the enhancement of solar simulated radiation (ssr) tumorigenesis by 5-methoxypsoralen (5-mop) in model perfumes that contain bergamot oil. this work was done in association with yeast mutagenicity studies and human skin phototoxicity studies. analyses of time-to-onset of tumour observation with 5-mop at 0, 5, 15 and 50 ppm show a highly significant 5-mop dose effect and the data indicate that 5-mop has phototumorigenic potent ... | 1990 | 2128326 |
| structure, expression and chromosomal localization of zfp-1, a murine zinc finger protein gene. | zinc finger proteins (zfp) are encoded by a large family of genes present in many organisms including yeast and human. some of them are transcriptional activators and bind specifically to dna by zinc mediated folded structures commonly known as zinc fingers. the drosophila krüppel (kr) is a segmentation gene and encodes a zinc finger protein. using a probe from the finger domain of kr, we have isolated a structurally related gene zfp-1 from the mouse. in this paper, we report the complete nucleo ... | 1989 | 2574853 |
| transport of the yeast atp synthase beta-subunit into mitochondria. effects of amino acid substitutions on targeting. | we have isolated the yeast atp2 gene encoding the beta-subunit of mitochondrial atp synthase and determined its nucleotide sequence. a fusion between the n-terminal 15 amino acid residues of beta-subunit and the mouse cytosolic protein dihydrofolate reductase (dhfr) was transcribed and translated in vitro and found to be transported into isolated yeast mitochondria. a fusion with the first 35 amino acid residues of beta-subunit attached to dhfr was not only transported but also proteolytically p ... | 1990 | 2138017 |
| transformation and ph homeostasis of fibroblasts expressing yeast h(+)-atpase containing site-directed mutations. | mouse fibroblasts expressing a yeast proton-pumping atpase show tumorigenic transformation (r. perona, and r. serrano, nature (london) 334:438-440, 1988). by expressing site-directed mutations of the yeast atpase with different levels of activity, a close correlation has been found between enzyme activity, tumorigenic transformation, and intracellular ph measured by weak-acid distribution. fibroblasts expressing the yeast proton-pumping atpase showed increased capability to grow at acidic ph and ... | 1990 | 2142513 |
| [the research institute for molecular pathology (i.m.p.)--a new approach for cancer development]. | the aim of basic research at the i.m.p. is a more comprehensive understanding of cancer development that should lead to less aggressive and more efficient therapies in future. molecular biological methods provide a valuable tool to describe the genetic defects and malfunctions of the cancer cell, an understanding of which is essential for the establishment of causal cancer treatment. several working groups are studying aspects of cellular growth and its regulation from different points of view. ... | 1989 | 2609663 |