| confirmed clinical neospora caninum infection in a boxer puppy from argentina. | generalized neosporosis was diagnosed in a 2-month-old boxer puppy. the dog had a history of progressive paralysis and muscle atrophy, followed by cervical weakness, stiff jaws and dysphagia. the dog had a 1:12,800 antibody titer for neospora caninum and was negative for antibodies to toxoplasma gondii by the indirect fluorescent antibody test (ifat). after euthanasia a complete necropsy was carried out. the puppy had a megaesophagus. microscopically, tachyzoites and tissue cysts were observed i ... | 2005 | 15978728 |
| maintenance of neospora caninum tachyzoites using mongolian gerbils (meriones unguiculatus). | | 1999 | 16032067 |
| immunological relationship between neospora caninum and besnoitia besnoiti. | neospora caninum is a coccidian parasite identified as a major cause of abortion in cattle. a combined infection of n. caninum with another taxonomically related parasite of cattle, besnoitia besnoiti can occur in geographical areas endemic for both species. both infections are routinely diagnosed serologically, and incorrect diagnosis could occur if immunological cross-reactivity exists between the two parasites. to investigate the possible degree of cross-reactivity, we compared results obtain ... | 2002 | 11992709 |
| first isolation of neospora caninum from the feces of a naturally infected dog. | neospora caninum is a major cause of abortion in cattle worldwide. cattle become infected with n. caninum by ingesting oocysts from the environment or transplacentally from dam to fetus. experimentally, dogs can act as definitive hosts, but dogs excrete few oocysts after ingesting tissue cysts. a natural definitive host was unknown until now. in the present study, n. caninum was isolated from the feces of a dog. gerbils (meriones unguiculatus) fed feces from the dog developed antibodies to n. ca ... | 2001 | 11426726 |
| characterization of the oregon isolate of neospora hughesi from a horse. | neospora hughesi was isolated in cell cultures inoculated with homogenate of spinal cord from a horse in oregon. tachyzoites of this oregon isolate of n. hughesi were maintained continuously by cell culture passage and tachyzoites were infective to immunosuppressed mice. gamma interferon gene knockout (ko) mice injected with tachyzoites developed fatal myocarditis and numerous tachyzoites were seen in lesions. gerbils (meriones unguiculatus) inoculated with tachyzoites developed antibodies (> or ... | 2001 | 11318565 |
| gerbils (meriones unguiculatus) are highly susceptible to oral infection with neospora caninum oocysts. | laboratory-reared gerbils (meriones unguiculatus) were found to be highly susceptible to oral infection with neospora caninum (nc-liv strain) oocysts. gerbils fed approximately 1000 oocysts became sick or died at 6-13 days post feeding of oocysts (pfo). n. caninum was isolated in cell culture and from gamma-interferon-knockout mice inoculated with homogenates of mesenteric lymph nodes of gerbils examined as early as 1 day pfo. numerous n. caninum tachyzoites were found in ulcerative lesions in t ... | 2000 | 10685849 |
| characterization of tissue distribution and histopathological lesions in neospora caninum experimentally infected gerbils. | gerbils (meriones unguiculatus) were inoculated intraperitoneally (i.p.) with neospora caninum tachyzoites to examine parasite distribution and histological lesions at different time points over a 9-day period of infection. gerbils were sacrificed 12 h post-infection (pi), then daily intervals up to day 9 pi. the parasite was detected by pcr assay targeting the nc5 sequence of n. caninum. the parasite was not found in any organs until day 5 pi, however, from day 8 pi onwards, they were detected ... | 2009 | 19172297 |
| loss of infectivity of neospora caninum oocysts maintained for a prolonged time. | the purpose of this study was to investigate whether sporulated neospora caninum oocysts, which had been stored for 46 mo in a 2% sulfuric acid solution at 4 degrees c, remain morphologically viable and infective to gerbils (meriones unguiculatus). six gerbils were orally inoculated with doses of 400 or 1,200 oocysts. two mo after inoculation, the animals did not show any clinical signs, had no histological lesions, and were seronegative for n. caninum at 1: 50 in an immunofluorescent antibody t ... | 2007 | 18165712 |
| incidence of neospora caninum and other intestinal protozoan parasites in populations of swiss dogs. | the protozoan parasite neospora caninum is one of the most important abortifacient organisms in cattle worldwide. the dog is known to act as definitive host although its potential role as infection source for bovines still remains unelucidated. the aim of the present study was to compile initial epidemiological data on the prevalence and incidence of n. caninum in swiss dogs acting as definitive hosts. thus, 249 swiss dogs were investigated coproscopically in monthly intervals over a period of 1 ... | 2006 | 16564131 |
| gerbil model of acute neosporosis. | experimental infections with the nc-1 strain of neospora caninum were conducted in gerbils (meriones unguiculatus) to determine their acute responses to experimental intraperitoneal infection. five groups of five female gerbils were used and they were intraperitoneally infected with 1x10(6), 2x10(6), 3x10(6), 4x10(6) or 5x10(6) tachyzoites. gerbils in all groups developed clinical signs of neosporosis which consisted of inactivity 4-5 days post-inoculation. morbidity and mortality were observed ... | 2005 | 15631903 |