| isolation and characterization of a new insect flavivirus from aedes albopictus and aedes flavopictus mosquitoes in japan. | we isolated a new flavivirus from aedes albopictus mosquito and a related species in japan. the virus, designated aedes flavivirus (aefv), only replicated in a mosquito cell line and produced a mild cytopathic effect. the aefv genome was positive-sense, single-stranded rna, 11,064 nucleotides in length and contained a single open reading frame encoding a polyprotein of 3341 amino acids with 5' and 3' untranslated regions (utrs) of 96 and 945 nucleotides, respectively. genetic and phylogenetic an ... | 2009 | 19580982 |
| arboviral survey of mosquitoes in two northern italian regions in 2007 and 2008. | recently, italy-particularly the emilia-romagna region-was the location of consecutive outbreaks of human diseases caused by the arboviruses chikungunya virus and west nile virus. the two outbreaks, spread by different species of mosquitoes, were not related, but pointed out the lack of an arboviral surveillance program in this region. beginning in 2007 entomological surveillance was initiated in the emilia-romagna region, and in 2008 the program was improved and extended at lombardia region. us ... | 2010 | 20370434 |
| molecular characterization of flaviviruses from field-collected mosquitoes in northwestern italy, 2011-2012. | the genus flavivirus comprises several mosquito-borne species, including the zoonotic pathogens west nile and usutu virus, circulating in animals and humans in italy since 1998. due to its ecological and geographical features, piedmont is considered a risk area for flavivirus transmission. here we report the results of a flavivirus survey (detection and genetic characterization) of mosquitoes collected in piedmont in 2012 and the genetic characterization of three strains detected in 2011. | 2014 | 25160565 |
| detection of a new insect flavivirus and isolation of aedes flavivirus in northern italy. | during recent years, numerous novel 'insect flaviviruses' have been discovered in natural mosquito populations. in a previous study we described the presence of flavivirus dna sequences integrated in aedes albopictus (asian tiger mosquito) populations from northern italy in 2007. | 2012 | 23043958 |
| detection of mosquito-only flaviviruses in europe. | the genus flavivirus, family flaviviridae, includes a number of important arthropod-transmitted human pathogens such as dengue viruses, west nile virus, japanese encephalitis virus and yellow fever virus. in addition, the genus includes flaviviruses without a known vertebrate reservoir, which have been detected only in insects, particularly in mosquitoes, such as cell fusing agent virus, kamiti river virus, culex flavivirus, aedes flavivirus, quang binh virus, nakiwogo virus and calbertado virus ... | 2012 | 22377581 |
| wide detection of aedes flavivirus in north-eastern italy--a european hotspot of emerging mosquito-borne diseases. | the pattern of flavivirus infection in mosquitoes belonging to the genera aedes and culex collected in two regions of north-eastern italy (trentino and veneto) was assessed. mosquitoes were collected during 2012 and screened for flaviviruses using a generic reverse transcription-nested-pcr targeted on a region of the non-structural ns5 gene. the phylogenetic analysis was performed on a fragment of ~1000 bp. virus isolation was attempted in c6/36 insect cell lines and the infected cell cultures w ... | 2015 | 25326313 |
| complete genome sequence of menghai flavivirus, a novel insect-specific flavivirus from china. | menghai flavivirus (mfv) was isolated from aedes albopictus in menghai county of yunnan province, china, during an arboviruses screening program in august 2010. whole genome sequencing of mfv was performed using an ion pgm™ sequencer. the complete genome of mfv was 10897 nucleotides in length and encoded a polyprotein and fairly interesting flavivirus orf (fifo). the polyprotein contained three flavivirus structural proteins (c, prm/m and e) and seven nonstructural proteins. nucleotide blast ana ... | 2017 | 28175982 |
| first isolation of aedes flavivirus in the western hemisphere and evidence of vertical transmission in the mosquito aedes (stegomyia) albopictus (diptera: culicidae). | we report here the first evidence of vertical transmission of aedes flavivirus (aefv) and its first isolation in the western hemisphere. aefv strain spfld-mo-2011-mp6 was isolated in c6/36 cells from a pool of male aedes albopictus mosquitoes that were reared to adults from larvae collected in southwest missouri, usa, in 2011. electron micrographs of the virus showed virions of approximately 45nm in diameter with morphological characteristics associated with flaviviruses. the genomic sequence de ... | 2013 | 23582303 |