development of a real-time qpcr assay for the detection of rv2 lineage-specific rhadinoviruses in macaques and baboons. | two distinct lineages of rhadinoviruses related to kaposi's sarcoma-associated herpesvirus (kshv/hhv8) have been identified in macaques and other old world non-human primates. we have developed a real-time quantitative pcr (qpcr) assay using a taqman probe to differentially detect and quantitate members of the rhadinovirus-2 (rv2) lineage. pcr primers were derived from sequences within orf 60 and the adjacent orf 59/60 intergenic region which were highly conserved between the macaque rv2 rhadino ... | 2005 | 15634356 |