| comparison between human and armadillo paracoccidioides brasiliensis isolates by random amplified polymorphic dna analysis. | sixty-three paracoccidioides brasiliensis isolates obtained from three nine-banded armadillos (dasypus novemcinctus), one amazonian armadillo's and 19 clinical isolates were compared by random amplified polymorphic dna analysis with the primer opg-19. the isolates were divided into three major clusters, i, ii and iii. coincidences between human and armadillo isolates were observed in clusters i and ii. cluster iii consisted only of armadillos' isolates. the results suggested that (i) humans may ... | 1998 | 10353214 |
| cerebriform colonies of paracoccidioides brasiliensis isolated from nine-banded armadillos (dasypus novemcinctus) at room temperature. | twelve isolates of paracoccidioides brasiliensis generated cerebriform colonies at room temperature on potato glucose agar slants (pda). these isolates contained abundant chlamydospores and yeast-like cells and are a subset of the 65 isolates obtained from nine-banded armadillos (dasypus novemcinctus). they grew as a yeast form with typical multiple buddings at 37 degrees c on brain heart infusion agar supplemented with 1% glucose. after replating on pda and culturing at room temperature for 2 m ... | 2001 | 11398643 |
| biosynthesis of chondroitinase and hyaluronidase by different strains of paracoccidioides brasiliensis. | the biosynthesis of chondroitinase and hyaluronidase by different isolates of paracoccidioides brasiliensis was investigated in 20 strains isolated from patients (17 strains), a penguin (pygocelis adeliae, one strain), an armadillo (dasypus novemcinctus, one strain) and the environment (dog food, one strain). all the p. brasiliensis isolates studied had the ability to produce chondroitinase and hyaluronidase, although differences in colony morphology and enzyme production were detected among the ... | 2003 | 12748266 |
| comparison of the sequences of the internal transcribed spacer regions and pbgp43 genes of paracoccidioides brasiliensis from patients and armadillos (dasypus novemcinctus). | paracoccidioides brasiliensis isolates from 10 nine-banded armadillos (dasypus novemcinctus) were comparable with 19 clinical isolates by sequence analysis of the pbgp43 gene and ribosomal internal transcribed spacer 1 (its1) and its2 and by random amplified polymorphic dna. in this original its study, eight isolates differed by one or three sites among five total substitution sites. | 2003 | 14662970 |
| igm and igg antibody response to paracoccidioides brasiliensis in naturally infected wild armadillos (dasypus novemcinctus). | we studied the extent to which wild nine-banded armadillos, dasypus novemcinctus, produce immune humoral responses specifically directed against characteristic paracoccidioides brasiliensis antigens. such antibody production might reflect direct contact with the ecological microniche of p. brasiliensis, or might merely reflect inhalation of widely distributed airborne propagules. an enzyme-linked immunosorbent assay (elisa) was designed containing purified glycoprotein gp43 and gp70 antigens fro ... | 2004 | 15473362 |
| molecular detection of paracoccidioides brasiliensis in road-killed wild animals. | paracoccidioides brasiliensis infections have been little studied in wild and/or domestic animals, which may represent an important indicator of the presence of the pathogen in nature. road-killed wild animals have been used for surveillance of vectors of zoonotic pathogens and may offer new opportunities for eco-epidemiological studies of paracoccidiodomycosis (pcm). the presence of p. brasiliensis infection was evaluated by nested-pcr in tissue samples collected from 19 road-killed animals; 3 ... | 2008 | 17885959 |
| diversity in paracoccidioides brasiliensis. the pbgp43 gene as a genetic marker. | paracoccidioides brasiliensis is a temperature-dependent dimorphic fungus and the agent of paracoccidioidomycosis (pcm), which is prevalent in rural workers of latin american countries. until a decade ago, most of the studies involving p. brasiliensis used clinical isolates, since environmental samples from soil are difficult to obtain. more recently, p. brasiliensis has been isolated from infected wild and domestic animals, especially from the nine-banded armadillo dasypus novemcinctus in brazi ... | 2008 | 18777634 |
| virulence profiles of ten paracoccidioides brasiliensis isolates obtained from armadillos (dasypus novemcinctus). | paracoccidioides brasiliensis is the etiologic agent of paracoccidioidomycosis (pcm), the most important systemic mycosis in latin america. the armadillo, dasypus novemcinctus, has been confirmed as the primary natural reservoir of this fungus. its geographic distribution is similar to that of human pcm. in this study, virulence profiles of 10 p. brasiliensis isolates from different armadillos and of two clinical isolates were tested in an experimental hamster model. pathogenicity was evaluated ... | 2003 | 12964840 |
| high frequency of paracoccidioides brasiliensis infection in armadillos (dasypus novemcinctus): an ecological study. | the fungus paracoccidioides brasiliensis has been isolated from nine-banded armadillos (dasypus novemcinctus) in different regions where paracoccidiodomycosis (pcm) is endemic. the link between pcm and these animals has provided the first valuable clue in the effort to elucidate the ecological niche of p. brasiliensis. the present study was aimed at correlating p. brasiliensis infection in armadillos with local ecological features and, if possible, the presence of the fungus in the soil in the b ... | 2003 | 12964713 |
| the habitat of paracoccidioides brasiliensis: how far from solving the riddle? | when trying to understand the pathophysiology of any infectious agent, one key piece of information is the determination of its habitat. in the case of paracoccidioides brasiliensis, the precise location of the fungus' environmental niche remains undefined despite the efforts of various research groups. this review summarizes recent studies on the ecology of p. brasiliensis and certain facets of paracoccidioidomycosis. studies on the juvenile form of paracoccidioidomycosis in children less than ... | 2001 | 11446526 |
| isolation of paracoccidioides brasiliensis from armadillos (dasypus novemcinctus) in an area where the fungus was recently isolated from soil. | natural infection of armadillos (dasypus novemcinctus) with paracoccidioides brasiliensis in northern brazil was reported in 1986, raising great interest in the understanding of the role of this mammal in the epidemiological cycle of the fungus. recently, p. brasiliensis was isolated from the soil of ibiá, state of minas gerais, southeastern brazil. armadillos captured in this area were evaluated for the presence of p. brasiliensis in the viscera and infection was detected in 4/16 animals (25%). ... | 2000 | 10892986 |
| pathogenicities and gp43kda gene of three paracoccidioides brasiliensis isolates originated from a nine-banded armadillo (dasypus novemcinctus). | we studied three different isolates of paracoccidioides brasiliensis obtained from the mesenteric lymph node (d3ly1), the spleen (d3s1) and the liver (d3liv1) of the same armadillo (dasypus novemcinctus). pulmonal inflammatory area was evaluated by intravenous inoculation of 10(6) yeast cells of each isolates in young, male, ddy mice. moreover, the partial sequence of gp43kda gene of p. brasiliensis was analyzed. the lung inflammatory area was greater in animals inoculated with isolate d3s1. the ... | 1998 | 10481285 |
| isolation of paracoccidioides brasiliensis from armadillos (dasypus noveminctus) captured in an endemic area of paracoccidioidomycosis. | paracoccidioides brasiliensis, the causative agent of paracoccidioidomycosis (pcm), was first isolated from armadillos from the amazonian region where the mycosis is uncommon. in the present study, we report on the high incidence of pcm infection in armadillos from a hyperendemic region of the disease. four nine-banded armadillos (dasypus novemcinctus) were captured in the endemic area of botucatu, sao paulo, brazil, killed by manual cervical dislocation and autopsied under sterile conditions. f ... | 1998 | 9574800 |
| importance of xenarthrans in the eco-epidemiology of paracoccidioides brasiliensis. | several pathogens that cause important zoonotic diseases have been frequently associated with armadillos and other xenarthrans. this mammal group typically has evolved on the south american continent and many of its extant species are seriously threatened with extinction. natural infection of armadillos with paracoccidioides brasiliensis in hyperendemic areas has provided a valuable opportunity for understanding the role of this mammal in the eco-epidemiology of paracoccidioidomycosis (pcm), one ... | 2009 | 19919716 |
| experimental infections with paracoccidioides brasiliensis obtained from armadillos: comparison to clinical isolates. | paracoccidioides brasiliensis causes paracoccidioidomycosis (pcm) that is one of the most prevalent systemic human mycoses in latin america. armadillos show a high incidence of pcm infection and could, therefore, be a natural reservoir for this fungus. in this study were compared the virulence profiles of isolates obtained from nine-banded armadillos (dasypus novemcinctus) (pbt1 and pbt4) and isolates from pcm patients (pb265 and bt83). pathogenicity was evaluated by fungal load and analysis of ... | 2008 | 18553016 |
| isolation of paracoccidioides brasiliensis from the nine-banded armadillo dasypus novemcinctus, in an endemic area for paracoccidioidomycosis in colombia. | the microniche of the dimorphic fungus paracoccidiodes brasiliensisremains undefined in spite of the many attempts to isolate it from natural sources. until recently, knowledge was also scanty concerning the presence of natural infections in animals; however, in the last decade, the fungus has been repeatedly isolated from the nine-banded armadillo, dasypus novemcinctusin brazil. a study aimed at determining the presence of infected armadillos in one of the paracoccidioidomycosis endemic areas o ... | 1999 | 18473551 |
| phylogenetic and evolutionary aspects of paracoccidioides brasiliensis reveal a long coexistence with animal hosts that explain several biological features of the pathogen. | the habitat of the mycelial saprobic form of paracoccidioides brasiliensis, which produces the infectious propagula, has not been determined and has proven difficult for mycologists to describe. the fungus has been rarely isolated from the environment, the disease has a prolonged latency period and no outbreaks have been reported. these facts have precluded the adoption of preventive measures to avoid infection. the confirmation of natural infections in nine-banded armadillos (dasypus novemcinct ... | 2006 | 16473563 |
| the naked-tailed armadillo cabassous centralis (miller 1899): a new host to paracoccidioides brasiliensis. molecular identification of the isolate. | the natural habitat of paracoccidioides brasiliensis remains undefined but the repeated demonstration of infection by this fungus in the nine-banded armadillo dasypus novemcinctus has opened interesting research avenues. we report here the isolation of this fungus from the spleen of a naked-tailed armadillo cabassous centralis (miller 1899) captured in a coffee farm localized in the colombian endemic area for paracoccidioidomycosis. this particular isolate was identified by its dimorphism and al ... | 2005 | 16010854 |
| role of the armadillo dasypus novemcinctus in the epidemiology of paracoccidioidomycosis. | a study conducted in a rural area of ibiá, state of minas gerais, brazil, where paracoccidioides brasiliensis was recently isolated from soil, sought to determine if the armadillo dasypus novemcinctus developed paracoccidioidomycosis. out of 21 armadillos captured in the area, one had a lung granuloma containing fungal cells attributable to those of p. brasiliensis. the present report presents the first histopathological evidence for the presence of this pathogen in the lungs of an armadillo. th ... | 1998 | 10531678 |