lipid-containing bacteriophage pr4: structure and life cycle.the structure of the purified lipid-containing phage pr4 was studied electron microscopically using thin sectioning, negative staining and freeze-fracturing techniques. the lipid layer was located inside a rigid capsid and had a bilayer structure. the innermost dense core was probably composed solely of dna since no major structural proteins were missing in empty phage particles lacking dna. during infection, phages were attached to the cell wall of the host. they apparently inject their dna thr ...1979113500
the lipid-containing bacteriophage pr4. effects of altered lipid composition on the virion.phage pr4 was grown on a variety of escherichia coli mutants defective in fatty acid and phospholipid metabolism. the composition of the phage lipids was modified by changing the composition of the host membrane phospholipids. the compositions of both the polar and the acyl moieties of the phospholipids were altered. the proportion of saturated fatty acids in the phage phospholipids was increased in increments from 44% of the total fatty acids to 55%, 61% and 69% of the total fatty acids using a ...19836341607
inhibitory effect of fatty acids on the entry of the lipid-containing bacteriophage pr4 into escherichia coli.various unsaturated fatty acids (notably palmitoleic acid and oleic acid) interfered with plaque production by the lipid-containing bacteriophage pr4 on lawns of escherichia coli. addition of fatty acid to give 50 mug/ml ( approximately 0.2 mm) at the time of infection prevented phage replication. if, however, the fatty acid was added after infection, normal amounts of phage were produced. if the fatty acid was added (to 50 mug/ml) to the host cell culture a long enough time before infection suc ...1978342724
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