| cloning of a human parvovirus by molecular screening of respiratory tract samples. | the identification of new virus species is a key issue for the study of infectious disease but is technically very difficult. we developed a system for large-scale molecular virus screening of clinical samples based on host dna depletion, random pcr amplification, large-scale sequencing, and bioinformatics. the technology was applied to pooled human respiratory tract samples. the first experiments detected seven human virus species without the use of any specific reagent. among the detected viru ... | 2005 | 16118271 |
| human bocavirus in children. | | 2006 | 16710957 |
| frequent detection of human rhinoviruses, paramyxoviruses, coronaviruses, and bocavirus during acute respiratory tract infections. | viruses are the major cause of pediatric acute respiratory tract infection (arti) and yet many suspected cases of infection remain uncharacterized. we employed 17 pcr assays and retrospectively screened 315 specimens selected by season from a predominantly pediatric hospital-based population. before the brisbane respiratory virus research study commenced, one or more predominantly viral pathogens had been detected in 15.2% (n = 48) of all specimens. the brisbane study made an additional 206 vira ... | 2006 | 16847968 |
| real-time pcr assays for detection of bocavirus in human specimens. | the recently discovered human bocavirus (hbov) is the first member of the family parvoviridae, genus bocavirus, to be potentially associated with human disease. several studies have identified hbov in respiratory specimens from children with acute respiratory disease, but the full spectrum of clinical disease and the epidemiology of hbov infection remain unclear. the availability of rapid and reliable molecular diagnostics would therefore aid future studies of this novel virus. to address this, ... | 2006 | 16954253 |
| detection of bocavirus dna in nasopharyngeal aspirates of a child with bronchiolitis. | we describe a case of bronchiolitis associated with the newly detected human bocavirus (hbov) in a child with a suspected noonan syndrome. this is the first report of a bronchiolitis probably linked to hbov that required intensive care while being accompanied by a congenital heart disease and a history of several episodes of severe respiratory symptoms. | 2007 | 16968654 |
| human bocavirus: developing evidence for pathogenicity. | | 2006 | 17041844 |
| severe pneumonia and human bocavirus in adult. | | 2006 | 17176591 |
| high prevalence of human bocavirus detected in young children with severe acute lower respiratory tract disease by use of a standard pcr protocol and a novel real-time pcr protocol. | the human bocavirus (hbov) was recently isolated from respiratory tract samples. within a study collective of children with severe lower respiratory tract disease, the patients testing positive for hbov (12.8%) had a higher rate of underlying cardiopulmonary disease. viral loads in respiratory tract specimens varied from 10(2) to 10(10) genome equivalents/ml. | 2007 | 17215343 |
| isolation of human bocavirus from swiss infants with respiratory infections. | human bocavirus (hbov) is a novel agent associated with respiratory symptoms in adults and children. we studied prospectively the first acute respiratory infection in a birth cohort of healthy neonates in switzerland. hbov was identified in 5 (4.5%) of 112 infants as young as 3 months of age. in 4 of the 5 infants, hbov was associated with other respiratory viruses. we conclude that hbov circulates in the community and is acquired early in life. | 2007 | 17259883 |
| spectrum of viruses and atypical bacteria in intercontinental air travelers with symptoms of acute respiratory infection. | respiratory infections after air travel are frequent, but epidemiological data are incomplete. using sensitive polymerase chain reactions, we studied the spectrum of atypical bacteria and respiratory viruses in travelers fulfilling the case definition of severe acute respiratory syndrome. a pathogen was identified in 67 travelers (43.2%). influenza and parainfluenza viruses were most prevalent, at 14.2% and 15.5%, respectively. prevalences of adenoviruses, human metapneumovirus, coronaviruses, a ... | 2007 | 17262708 |
| human bocavirus: a novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in thailand. | we detected human bocavirus (hbov) infection in 4.5% of hospitalized patients with pneumonia in rural thailand. however, the role of hbov as a pathogen is unclear. | 2007 | 17330795 |
| human bocavirus in italian patients with respiratory diseases. | hbov, a recently discovered parvovirus, can be present in the respiratory tract of patients with acute respiratory diseases (ard), but its etiologic involvement in the underlying diseases is still uncertain. | 2007 | 17336143 |
| human bocavirus and acute wheezing in children. | human bocavirus is a newly discovered parvovirus. it has been detected primarily in children with acute lower respiratory tract infection, but its occurrence, clinical profile, and role as a causative agent of respiratory tract disease are not clear. | 2007 | 17342639 |
| frequent detection of the parvoviruses, parv4 and parv5, in plasma from blood donors and symptomatic individuals. | plasma pools used in the manufacture of blood- and plasma-derived medicinal products are frequently contaminated with parvovirus b19. the presence of the novel human parvovirus parv4 and a related variant parv5 in manufacturing plasma pools was recently demonstrated. another recently identified parvovirus, human bocavirus (hbov), has been identified in respiratory samples from children with lower respiratory tract disease. | 2007 | 17524097 |
| human bocavirus, a respiratory and enteric virus. | in spain, human bocavirus (hbov) was detected in 48 (9.1%) of 527 children with gastroenteritis at similar frequency as for children with respiratory illness (40/520, 7.7%). fecal excretion adds new concern about the transmission of hbov. to our knowledge, this report is the first to document hbov in human feces. | 2007 | 17553287 |
| [the human bocavirus: pathogen in airway infections?]. | the human bocavirus (hbov), the second member of the parvovirus family, which displays pathogenicity in humans, has been described in 2005 by allander et al.. it seems to be distributed worldwide and has been isolated mainly in infants and children with respiratory tract infection. this review covers all studies published on hbov to february 2007 and discusses this emerging viral pathogen from the perspective of inpatient medical treatment centers. | 2007 | 17607653 |
| newly discovered respiratory viruses: significance and implications. | with the recent advances in molecular biology and the ability to amplify viral genomes in a non-sequence-dependent manner, several previously unidentified human respiratory viruses have been discovered. there are accumulating data that some of these new pathogens are responsible for a substantial proportion of respiratory tract diseases, particularly in children. this review will focus on several of these newly identified pathogens for which there are clinical data implicating a role of these vi ... | 2007 | 17689145 |
| seroepidemiology of human bocavirus in hokkaido prefecture, japan. | a new human virus, provisionally named human bocavirus (hbov), was discovered by swedish researchers in 2005. a new immunofluorescence assay using trichoplusia ni insect cells infected with a recombinant baculovirus expressing the vp1 protein of hbov was developed, and the levels of immunoglobulin g antibody to the vp1 protein of hbov in serum samples were measured. the overall seroprevalence rate of antibodies against the vp1 protein of hbov in a japanese population aged from 0 months to 41 yea ... | 2007 | 17699639 |
| human bocavirus: multisystem detection raises questions about infection. | | 2007 | 17763314 |
| clinical and molecular epidemiology of human bocavirus in respiratory and fecal samples from children in hong kong. | human bocavirus (hbov) is a recently discovered parvovirus associated with respiratory tract infections in children. we conducted the first systematic prospective clinical and molecular study using nasopharyngeal aspirates (npas) and fecal samples. | 2007 | 17763318 |
| [human bocavirus infections in spanish 0-14 year-old: clinical and epidemiological characteristics of an emerging respiratory virus]. | in 2005 a new respiratory virus, called human bocavirus (hbov), was cloned from respiratory samples from swedish infants and children with lower respiratory tract infections. | 2007 | 17785157 |
| high incidence of human bocavirus infection in children in spain. | the newly identified human bocavirus (hbov), a member of the parvoviridae family, has been associated to low respiratory tract infections in young children. | 2007 | 17904416 |
| human bocavirus. | human bocavirus (hbov) was first described in 2005 in nasopharyngeal aspirates of children with respiratory tract infection. multiple studies have confirmed the presence of hbov in respiratory tract samples of children world-wide. hbov has recently also been detected in blood and fecal samples. most studies so far have studied virus prevalence, and only a few reports provide data regarding the linkage of hbov to disease. these reports indicate that hbov infection is indeed associated with acute ... | 2008 | 18055252 |
| respiratory virus surveillance and outbreak investigation. | sensitive, rapid detection of respiratory viruses is needed for surveillance and for investigation of epidemiologically linked cases. the utility of rapid antigen-based methods for detection of common respiratory viruses and to confirm the cause of outbreaks is well established. however, nucleic acid amplification tests (nats) offer some benefits above antigen or culture-based procedures, with the main advantages being sensitivity and range of pathogens detectable. it is important to understand ... | 2007 | 18162251 |
| human bocavirus (hbov) in thailand: clinical manifestations in a hospitalized pediatric patient and molecular virus characterization. | human bocavirus (hbov), a novel virus, which based on molecular analysis has been associated with respiratory tract diseases in infants and children have recently been studied worldwide. to determine prevalence, clinical features and perform phylogenetic analysis in hbov infected thai pediatric patients. | 2008 | 18164764 |
| human bocavirus: clinical significance and implications. | human bocavirus (hbov), a parvovirus, was discovered in 2005 with the use of nonspecific genome amplification techniques. since its discovery, hbov has been identified worldwide. this review will focus on the epidemiology and clinical features associated with hbov infection. | 2008 | 18197041 |
| serodiagnosis of human bocavirus infection. | a new human-pathogenic parvovirus, human bocavirus (hbov), has recently been discovered and associated with respiratory disease in small children. however, many patients have presented with low viral dna loads, suggesting hbov persistence and rendering polymerase chain reaction-based diagnosis problematic. moreover, nothing is known of hbov immunity. we examined hbov-specific systemic b cell responses and assessed their diagnostic use in young children with respiratory disease. | 2008 | 18199037 |
| elisas using human bocavirus vp2 virus-like particles for detection of antibodies against hbov. | human bocavirus (hbov) has been identified worldwide in children with lower respiratory tract infections with an incidence of approximately 2-11%. the role of hbov in pathogenesis, however, is largely unknown, and little is known about the epidemiology of the virus. to study the seroepidemiology of hbov infection, the capsid protein was expressed in insect cells. expression of the putative major capsid protein vp2 in insect cells led to the formation of virus-like particles exhibiting the typica ... | 2008 | 18289709 |
| [human bocavirus (hbov)]. | the human bocavirus (hbov) has been recently identified by means of molecular screening techniques in respiratory tract secretions from children with acute respiratory tract disease. this virus, which belongs to the parvoviridae family, has been detected worldwide with a 5 to 10% prevalence among children with upper or lower respiratory tract infections, essentially during the winter period. a seroepidemiological study has shown that almost all the children have antibodies to hbov by the age of ... | 2009 | 18343604 |
| development of real-time pcr assays for detection and quantification of human bocavirus. | human bocavirus (hbov) is a parvovirus that has been recently detected in patients with respiratory illness. | 2008 | 18378492 |
| human bocavirus: passenger or pathogen in acute respiratory tract infections? | human bocavirus (hbov) is a newly identified virus tentatively assigned to the family parvoviridae, subfamily parvovirinae, genus bocavirus. hbov was first described in 2005 and has since been detected in respiratory tract secretions worldwide. herein we review the literature on hbov and discuss the biology and potential clinical impact of this virus. most studies have been pcr based and performed on patients with acute respiratory symptoms, from whom hbov was detected in 2 to 19% of the samples ... | 2008 | 18400798 |
| seroepidemiology of human bocavirus defined using recombinant virus-like particles. | human bocavirus (hbov) is a newly identified human parvovirus for which seroepidemiology and antigenic properties remain undefined. | 2008 | 18491974 |
| clinical and epidemiologic characteristics of human bocavirus in danish infants: results from a prospective birth cohort study. | human bocavirus (hbov) is a recently discovered parvovirus that has been detected in respiratory samples from children with acute respiratory tract infection (arti) and in feces from children with gastroenteritis. however, its role as a causative agent of respiratory disease is not determined. | 2008 | 18756188 |
| the challenge of respiratory virus infections in hematopoietic cell transplant recipients. | respiratory virus infections in hematopoietic cell transplant (hct) recipients are a major cause of morbidity and mortality. while respiratory syncytial virus (rsv), human metapneumovirus, parainfluenzaviruses, and influenza viruses are well known for their potential to cause fatal pneumonia, information has only recently emerged regarding the significance of the newly discovered viruses, such as human coronaviruses nl63 and hku1, and human bocavirus. lymphopenia seems to be the most important r ... | 2008 | 18785968 |
| clinical and epidemiological aspects of human bocavirus infection. | human bocavirus was recently described as a novel member of the parvoviridae to infect humans. based on accumulating clinical and epidemiological data the virus is currently being associated with respiratory infections in young children and infants and is furthermore discussed as causative agent of gastrointestinal illness. | 2008 | 18823816 |
| human bocavirus infection in children with respiratory tract disease. | human bocavirus (hbov) is a ubiquitous, newly described member of the parvoviridae family frequently detected in the respiratory tract of children, but only few reports provide data proving the link between hbov and respiratory tract disease (rtd). | 2008 | 18833027 |
| induced sputum in the diagnosis of childhood community-acquired pneumonia. | the usefulness of induced sputum in searching for causative agents of pneumonia in children has not been studied. | 2009 | 19052043 |
| absence of human bocavirus in bronchoalveolar lavage fluid of lung transplant patients. | | 2009 | 19083266 |
| [seroprevalence of antibody against human bocavirus in beijing, china]. | to find out the importance of human bocavirus (hbov) as an infectious agent for population in beijing, china, seroprevalence study was conducted by using expressed recombinant major capsid vp2 protein as an antigen. | 2008 | 19099684 |
| detection of human bocavirus in hospitalised children. | the objectives of this study are to assess the frequency of human bocavirus (hbov) infection in hospitalised children and to study the clinical symptoms associated with the detection of hbov. | 2009 | 19210599 |
| molecular characterization of infectious clones of the minute virus of canines reveals unique features of bocaviruses. | minute virus of canines (mvc) is a member of the genus bocavirus in the family parvoviridae. we have molecularly cloned and sequenced the 5'- and 3'-terminal palindromes of mvc. the mvc genome, 5,404 nucleotides (nt) in length, shared an identity of 52.6% and 52.1% with that of human bocavirus and bovine parvovirus, respectively. it had distinct palindromic hairpins of 183 nt and 198 nt at the left-end and right-end termini of the genome, respectively. the left-end terminus was also found in two ... | 2009 | 19211770 |
| novel application for isothermal nucleic acid sequence-based amplification (nasba). | human bocavirus (hbov), solely based on phylogenetic analyses, was classified as the second autonomous human parvovirus. unfortunately, neither susceptible cell cultures nor animal models were described hitherto, thus complicating studies on viral genome structure and replication steps. a novel application of nucleic acid sequence-based amplification (nasba) revealed that in all tested samples (100%) that became positive by nasba the negative strand of the hbov genome was packaged. additionally, ... | 2009 | 19428591 |
| human bocavirus can be cultured in differentiated human airway epithelial cells. | in 2005, a human bocavirus was discovered in children with respiratory tract illnesses. attempts to culture this virus on conventional cell lines has failed thus far. we investigated whether the virus can replicate on pseudostratified human airway epithelium. this cell culture system mimics the human airway environment and facilitates culturing of various respiratory agents. the cells were inoculated with human bocavirus-positive nasopharyngeal washes from children, and virus replication was mon ... | 2009 | 19474096 |
| kawasaki disease lacks association with human coronavirus nl63 and human bocavirus. | | 2009 | 19504744 |
| [human bocavirus]. | human bocavirus (hbov) is a newly identified parvovirus associated with acute respiratory infections in young children in different parts of the world. it is not inconceivable that this virus is also capable of causing acute gastroenteritis and asymptomatically persisting in infected children. hbov is the third widespread human respiratory virus after respiratory syncytial virus and rhinovirus. polymerase chain reaction remains the most reliable of hbov detection in clinical samples. phylogeneti ... | 2009 | 19537089 |
| [detection of human metapneumovirus and human bocavirus in children with bronchiolitis in east guangdong area]. | | 2009 | 19555574 |
| prevalence of human bocavirus in human tonsils and adenoids. | | 2009 | 19624951 |
| detection and quantification of human bocavirus in river water. | human bocavirus (hbov) was recently discovered in children with respiratory-tract infection and has been detected frequently in faecal specimens from children with gastroenteritis. the present study addresses for the first time, to our knowledge, the prevalence of hbov in river water. by using a newly developed real-time pcr targeting a conserved region of the np1 gene of hbov, virus levels in water samples were determined. moreover, partial sequence analysis of the np1 gene of hbov and comparat ... | 2009 | 19656966 |
| simultaneous detection of human bocavirus and adenovirus by multiplex real-time pcr in a belgian paediatric population. | since the discovery of human bocavirus (hbov), the virus has been detected worldwide in respiratory tract samples from young children by various polymerase chain reaction (pcr) assays and real-time pcrs (q-pcr). until now, no data have been reported on the presence of hbov in belgium and the detection of hbov in a multiplex q-pcr setting has not been described. the aim of this study was to develop a fast and reliable multiplex q-pcr for the simultaneous detection of hbov dna and adenovirus (adv) ... | 2009 | 19705175 |
| absence of detectable replication of human bocavirus species 2 in respiratory tract. | human bocavirus (hbov) commonly infects young children and is associated with respiratory disease; disease associations of the divergent hbov-2 species are unknown. frequent hbov-2 detection in fecal samples indicated widespread circulation in the united kingdom and thailand, but its lack of detection among 6,524 respiratory samples indicates likely differences from hbov-1 in tropism/pathogenesis. | 2009 | 19788826 |
| [diagnosis and molecular epidemiology of viral gastroenteritis in the past, present and future]. | outline, history of research, diagnosis and molecular epidemiology of viral gastroenteritis were described. rotavirus, adenovirus, norovirus, sapovirus, astrovirus, human parechovirus, aichivirus, and human bocavirus are the major target viruses which cause acute gastroenteritis. the viruses were differentiated into genogroup, genotypes and subgenotypes/clusters/lineages. the changing of their genetic backgrounds was well recognized in different areas and years. some reassortments or recombinati ... | 2009 | 19927992 |
| rapid molecular evolution of human bocavirus revealed by bayesian coalescent inference. | human bocavirus (hbov) is a linear single-stranded dna virus belonging to the parvoviridae family that has recently been isolated from the upper respiratory tract of children with acute respiratory infection. all of the strains observed so far segregate into two genotypes (1 and 2) with a low level of polymorphism. given the recent description of the infection and the lack of epidemiological and molecular data, we estimated the virus's rates of molecular evolution and population dynamics. a data ... | 2010 | 19932194 |
| heads up on human bocavirus. | | 2010 | 20083987 |
| [human bocavirus: studies in the literature and in chile]. | the human bocavirus (hbov), virus of the parvoviridae family, discovered by molecular methods in 2005, has been reported in respiratory samples, stool, urine and blood, both in children and adults. prevalence rates range from 0.8% in fecal samples of individuals with acute diarrhea, up to 19% in respiratory samples and blood. hbov has been detected in up to 43% of nasopharyngeal samples in asymptomatic children. in chile, hbov was detected in 24.2% of nasopharyngeal swabs in children under 5 yea ... | 2009 | 20098783 |
| human bocavirus in children with respiratory tract infection in shanghai: a retrospective study. | human bocavirus (hbov) was first reported in 2005. the worldwide presence of hbov in children with acute respiratory tract infection (arti) has been confirmed. this study aimed to understand the prevalence and clinical features of hbov in children with arti in shanghai and explore the causative implication of hbov in arti. | 2010 | 20143214 |
| dating of human bocavirus infection with protein-denaturing igg-avidity assays-secondary immune activations are ubiquitous in immunocompetent adults. | human bocavirus (hbov) is a widespread human parvovirus causing acute respiratory illness in young children. the hbov primary infections are viremic and can be diagnosed serologically. | 2010 | 20227338 |
| human bocavirus species 2 and 3 in brazil. | the newly described human bocavirus (hbov) species 2 and 3 have been repeatedly detected in stool strengthening the possibility that these viruses might present a tropism for the gastrointestinal tract and may be etiological agents of diarrhea. | 2010 | 20382557 |
| detection of novel respiratory viruses from influenza-like illness in the philippines. | several novel viruses have been recently identified in respiratory samples. however, the epidemiology of these viruses in tropical countries remains unclear. the aim of the present study was to provide an overview of the epidemiology of novel respiratory viruses, including human metapneumovirus, human bocavirus, new subtypes of human coronavirus (nl63 and hku1), ki virus, wu virus, and melaka virus in the philippines, a tropical country. nasopharyngeal aspirates from 465 patients with influenza- ... | 2010 | 20419824 |
| characterization of the gene expression profile of human bocavirus. | we have generated a quantitative transcription profile of human bocavirus type 1 (hbov1) by transfecting a nearly full-length clone in human lung epithelial a549 cells as well as in a replication competent system in 293 cells. the overall transcription profile of hbov1 is similar to that of two other members of genus bocavirus, minute virus of canines and bovine parvovirus 1. in particular, a spliced ns1-transcript that was not recognized previously expressed the large non-structural protein ns1 ... | 2010 | 20457462 |
| role of emerging respiratory viruses in children with severe acute wheezing. | acute wheezing episodes are frequently associated with respiratory viral infections in children. however, the role of the recently described respiratory viruses is not yet fully understood. | 2010 | 20503284 |
| human bocavirus in children. | | 2010 | 20508481 |
| newly identified respiratory viruses in children with asthma exacerbation not requiring admission to hospital. | there are few data describing the comprehensive identification in and influence of newly identified respiratory viruses on asthma exacerbations. most studies focus on inpatients. in this preliminary study, the point prevalence and the associations of picornavirus species described recently and human bocavirus (hbov) with the recovery from exacerbations in non-hospitalized asthmatic children (median age 5.1 years) were examined. human rhinoviruses (hrvs) were present in 52.6% of specimens, hbov-1 ... | 2010 | 20572080 |
| widespread infection with homologues of human parvoviruses b19, parv4, and human bocavirus of chimpanzees and gorillas in the wild. | infections with human parvoviruses b19 and recently discovered human bocaviruses (hbovs) are widespread, while parv4 infections are transmitted parenterally and prevalent specifically in injecting drug users and hemophiliacs. to investigate the exposure and circulation of parvoviruses related to b19 virus, parv4, and hbov in nonhuman primates, plasma samples collected from 73 cameroonian wild-caught chimpanzees and gorillas and 91 old world monkey (owm) species were screened for antibodies to re ... | 2010 | 20668071 |
| [expression of recombinant vp2 gene in insect sf9 cells and screening of clinical specimens]. | to clone and express vp, gene from hbov, and the expressed vp, protein was as the antigen in order to detect serum from children in wenling area with lower respiratory tract infections. | 2009 | 20718346 |
| [isolation and cell culture of human bocavirus (hbov) by human bronchial epithelial cell lines]. | to investigate pave a way for studying pathogenicty of hbov. | 2009 | 20718349 |
| human bocavirus in children: mono-detection, high viral load and viraemia are associated with respiratory tract infection. | human bocavirus 1 (hbov1) has recently been detected in children with respiratory tract infections (rti). in order to study whether hbov1 can cause rti, we investigated its presence in children with upper rti (urti), lower rti (lrti) and a control group of children without rti. | 2010 | 20833582 |
| allergic sensitization is associated with rhinovirus-, but not other virus-, induced wheezing in children. | data on the link between atopy and viral wheeze are limited. aim: to evaluate the association between ige sensitization and viral infection in wheezing children. | 2010 | 20977499 |
| identification and nearly full-length genome characterization of novel porcine bocaviruses. | the genus bocavirus includes bovine parvovirus (bpv), minute virus of canines (mvc), and a group of human bocaviruses (hbov1-4). using sequence-independent single primer amplification (sispa), a novel bocavirus group was discovered with high prevalence (12.59%) in piglet stool samples. two nearly full-length genome sequences were obtained, which were approximately 5,100 nucleotides in length. multiple alignments revealed that they share 28.7-56.8% dna sequence identity with other members of parv ... | 2010 | 21049037 |
| [clinical relevance of human bocavirus with acute respiratory tract infection and diarrhea in children: a prospective case-control study]. | to explore the causative role of human bocavirus (hbov) played in acute respiratory infection and diarrhea in children, a case-control study was prospectively conducted to investigate hbov detection in symptomatic children with acute respiratory tract infection, diarrhea and asymptomatic children. | 2010 | 21055300 |
| the detection of parvoviruses. | parvovirus b19 is a single-stranded dna virus which causes severe disease in immunocompromised patients and foetal loss in pregnant women. it is classified as an erythrovirus and this genus also comprises two related viral genotypes (so-called lali/a6 (genotype 2) and v9 (genotype 3)) which appear to be immunologically indistinguishable from parvovirus b19. serological and nucleic acid test (nat) systems to detect parvovirus b19-mediated infection are commercially available; however, some nat sy ... | 2011 | 21116804 |
| viruses and asthma. | background: viral respiratory infection has long been known to influence the occurrence of asthma exacerbations. over the last 20years much effort has been put into clarifying the role that viral respiratory infections play in the eventual development of asthma. scope of review: in this review we give a general background of the role of viruses in the processes of asthma exacerbation and asthma induction. we review recent additions to the literature in the last 3years with particular focus on cl ... | 2011 | 21291960 |
| molecular characterization of viruses associated with gastrointestinal infection in hiv-positive patients. | diarrhea is a major cause of morbidity and mortality among hiv-infected patients worldwide. | 2010 | 21340293 |
| detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern brazil. | the introduction of newer molecular methods has led to the discovery of new respiratory viruses, such as human metapneumovirus (hmpv) and human bocavirus (hbov), in respiratory tract specimens. we have studied the occurrence of hmpv and hbov in the porto alegre (pa) metropolitan area, one of the southernmost cities of brazil, evaluating children with suspected lower respiratory tract infection from may 2007-june 2008. a real-time polymerase chain reaction method was used for amplification and de ... | 2011 | 21340356 |
| phylogenetic and recombination analysis of human bocavirus 2. | human bocavirus 2(hbov2) and other human bocavirus species (hbov, hbov3, and hbov4) have been discovered recently. but the precise phylogenetic relationships among these viruses are not clear yet. | 2011 | 21345238 |
| kawasaki disease and human bocavirus-potential association? | kawasaki disease (kd) is an acute febrile multisystem vasculitic syndrome of unknown etiology, occurring mostly in infants and children younger than 5 years of age. we present a 13-month-old male with kd from whom was found human bocavirus dna in nasopharyngeal secretions. human bocavirus dna in a patient with kd raised question about the coincidental or possible etiological association. | 2011 | 21524620 |
| detection of ki polyomavirus and wu polyomavirus dna by real-time pcr in nasopharyngeal swabs and in normal lung and lung adenocarcinoma tissues. | polyomaviruses ki (kipyv) and wu (wupyv) were detected from 7 (3.0%) and 38 (16.4%) of 232 children with respiratory tract infections by real-time pcr. the rates of single infection of kipyv and wupyv were 3 (42.9%) of 7 and 20 (52.6%) of 38, respectively. in other samples, various viruses (human respiratory syncytial virus, human metapneumovirus (hmpv), human rhinovirus, parainfluenza virus 1 and human bocavirus) were detected simultaneously. one case was positive for kipyv and wupyv and also f ... | 2011 | 21545509 |
| human bocavirus and lower respiratory tract infection. | | 2011 | 21808965 |
| fatal type 7 adenovirus associated with human bocavirus infection in a healthy child. | adenovirus is a common cause of acute upper respiratory tract infection and rarely causes respiratory failure or septic shock in immunocompetent patients. severe disease is confined to immunocompromised patients. human bocavirus (hbov) is known as the fourth most common virus in respiratory samples from healthy subjects. in patients with respiratory complaints, it can be found alone or, more often, in combination with other viruses known to cause respiratory complaints. a well-documented fatal c ... | 2011 | 21837792 |
| detection of human bocavirus mrna in respiratory secretions correlates with high viral load and concurrent diarrhea. | human bocavirus (hbov) is a parvovirus recently identified in association with acute respiratory infections (ari). despite its worldwide occurrence, little is known on the pathogenesis of hbov infections. in addition, few systematic studies of hbov in ari have been conducted in latin america. therefore, in order to test whether active viral replication of human bocavirus is associated with respiratory diseases and to understand the clinical impact of this virus in patients with these diseases, w ... | 2011 | 21701591 |
| [genome cloning of human bocavirus (hbov1) and analysis of viral promoter activity]. | human bocavirus (hbov) is a recently discovered parvovirus, which is suspected to be an etiologic agent of respiratory disease and gastrointestinal disease in human. in the present study, we screened 941 nasopharyngeal aspirates collected from hospitalized children with lower respiratory tract infections from october 9, 2007 to march 20, 2009 in the children's hospital of hubei province. our results showed that 33 of 941 samples (3.51%) were detected positive for hbov. to obtain a full-length hb ... | 2011 | 22034820 |
| the prevalance of respiratory viruses among healthcare workers serving pilgrims in makkah during the 2009 influenza a (h1n1) pandemic. | despite the high risk of acquiring respiratory infections, healthcare workers who treat pilgrims at hajj have not been studied in previous research on respiratory diseases during hajj. the objective of this study was to determine the prevalence of different respiratory viruses among healthcare workers who treated pilgrims during hajj 2009, the year of the influenza a h1n1 pandemic. a cross-sectional study was performed just before and after hajj (25-29 november, 2009). nasal and throat swabs wer ... | 2011 | 22197024 |
| Viruses and bacteria in sputum samples of children with community-acquired pneumonia. | Clin Microbiol Infect ABSTRACT: Few comprehensive studies have searched for viruses and bacteria in children with community-acquired pneumonia (CAP). We identified 76 children hospitalized for pneumonia. Induced sputum samples were analysed for 18 viruses by antigen detection and PCR, and for six bacteria by culture and PCR. Viruses were found in 72% of samples, bacteria in 91%, and both in 66%. Rhinovirus (30%), human bocavirus (18%) and human metapneumovirus (14%) were the most commonly detect ... | 2011 | 21851481 |
| Evidence of human bocavirus viremia in healthy blood donors. | Human bocavirus DNA was detected by means of a quantitative, real-time polymerase chain reaction at low levels in the 5.51% of sera obtained from healthy blood donors, suggesting that viral detection in blood is not necessarily associated with disease status. | 2011 | 21996095 |
| detection of human bocavirus from children and adults with acute respiratory tract illness in guangzhou, southern china. | abstract: background: human bocavirus (hbov) is a newly discovered parvovirus associated with acute respiratory tract illness (arti) and gastrointestinal illness. our study is the first to analyze the characteristics of hbov-positive samples from arti patients with a wide age distribution from guangzhou, southern china. methods: throat swabs (n = 2811) were collected and analyzed from children and adults with arti over a 13-month period. the hbov complete genome from a 60 year-old female patien ... | 2011 | 22168387 |