| coronavirus hku1 infection in the united states. | in 2005, a new human coronavirus, hcov-hku1, was identified in hong kong. we screened respiratory specimens collected from december 16, 2001, to december 15, 2002, from children <5 years of age who tested negative for respiratory syncytial virus, parainfluenza viruses, influenza virus, and adenovirus for hcov-hku1 by reverse transcription-polymerase chain reaction. overall, 1,048 respiratory specimens from 851 children were tested, and 9 hcov-hku1-positive children (1%) were identified, 2 of who ... | 2006 | 16704837 |
| pan-viral screening of respiratory tract infections in adults with and without asthma reveals unexpected human coronavirus and human rhinovirus diversity. | between 50% and 80% of asthma exacerbations are associated with viral respiratory tract infections (rtis), yet the influence of viral pathogen diversity on asthma outcomes is poorly understood because of the limited scope and throughput of conventional viral detection methods. | 2007 | 17703411 |
| spike protein, s, of human coronavirus hku1: role in viral life cycle and application in antibody detection. | we recently described the discovery, genome, clinical features, genotypes and evolution of a novel and global human respiratory virus named human coronavirus hku1 (hcov-hku1) which is not yet culturable. we expressed a c-terminal flag-tagged cov-hku1 spike (s) protein by the semliki forest virus (sfv) system and investigated its maturation profile. pulse chase labeling revealed that s-flag was expressed as high-mannose n-glycans of monomers and trimers. it was predominantly cleaved into subdomai ... | 2008 | 18849544 |
| human coronavirus and acute respiratory illness in older adults with chronic obstructive pulmonary disease. | the clinical features and incidence of human coronavirus (hcov) infections in chronically ill older adults need better definition. | 2009 | 19239338 |
| epidemiology and clinical presentations of human coronavirus nl63 infections in hong kong children. | human coronavirus nl63 (hcov-nl63) has been found in children presenting with respiratory tract infections (rtis). however, the epidemiology and clinical course of this newly identified virus have not been fully elucidated. this study investigated the epidemiology, seasonality, and clinical features of hcov-nl63 in hong kong children. this study consisted of two cohorts of children hospitalized in a university-affiliated teaching hospital. in the 12-month retrospective part of the study, reverse ... | 2009 | 19759228 |
| a novel construction of genome space with biological geometry. | a genome space is a moduli space of genomes. in this space, each point corresponds to a genome. the natural distance between two points in the genome space reflects the biological distance between these two genomes. currently, there is no method to represent genomes by a point in a space without losing biological information. here, we propose a new graphical representation for dna sequences. the breakthrough of the subject is that we can construct the moment vectors from dna sequences using this ... | 2010 | 20360268 |
| detection of novel respiratory viruses from influenza-like illness in the philippines. | several novel viruses have been recently identified in respiratory samples. however, the epidemiology of these viruses in tropical countries remains unclear. the aim of the present study was to provide an overview of the epidemiology of novel respiratory viruses, including human metapneumovirus, human bocavirus, new subtypes of human coronavirus (nl63 and hku1), ki virus, wu virus, and melaka virus in the philippines, a tropical country. nasopharyngeal aspirates from 465 patients with influenza- ... | 2010 | 20419824 |
| prevalence and clinical characteristics of human cov-hku1 in children with acute respiratory tract infections in china. | human cov-hku1 (hcov-hku1) has been isolated from a 71-year-old man with pneumonia; however, the impact and role of emerging hcov-hku1 have not been defined in children with acute respiratory tract infection (arti). | 2010 | 20702134 |
| more and more coronaviruses: human coronavirus hku1. | after human coronaviruses oc43, 229e and nl63, human coronavirus hku1 (hcov-hku1) is the fourth human coronavirus discovered. hcov-hku1 is a group 2a coronavirus that is still not cultivable. the g + c contents of hcov-hku1 genomes are 32%, the lowest among all known coronaviruses with complete genome sequences available. among all coronaviruses, hcov-hku1 shows the most extreme codon usage bias, attributed most importantly to severe cytosine deamination. all hcov-hku1 genomes contain unique tan ... | 2009 | 21994538 |