| [effect of conditions and duration of aedes aegypti densonucleosis virus storage on its infective properties]. | | 1981 | 7253970 |
| densovirus of aedes aegypti as an expression vector in mosquito cells. | we have constructed an infectious dna clone containing the genome of aedes aegypti densovirus (aednv) in a bacterial plasmid. when this clone was transfected into aedes albopictus c6/36 mosquito cells, the aednv genome rescued from the plasmid and replicated as the wild-type virus. to investigate the cloned virus as an expression vector, the reporter gene encoding beta-galactosidase (beta-gal) was inserted into four large open reading frames (orf) observed in the aednv genome. when these recombi ... | 1994 | 7957754 |
| complete nucleotide sequence and genomic organization of the aedes albopictus parvovirus (aapv) pathogenic for aedes aegypti larvae. | we have cloned the replicative form of the aedes albopictus parvovirus (aapv) genome and determined the complete sequence of the viral strand. the sequence is 4176 nucleotides (nt) in length. the first 134 nt at the 3' end and the terminal 182 nt at the 5' end of the viral (minus) strand can both generate by folding and annealing of complementary sequences a typical terminal t-shaped structure although they differ in their sequence. three large open reading frames (orfs), each one in a different ... | 1994 | 8178459 |
| packaging of aednv-gfp transducing virus by expression of densovirus structural proteins from a sindbis virus expression system. | genetic recombination resulting in the production of wild-type infectious virus is an obstacle in the current system for producing densovirus transducing particles. in order to eliminate this problem, a double subgenomic sindbis virus (te/3'2j/vp) was engineered that expresses the structural proteins (vps) of aedes densonucleosis virus (aednv) from the second subgenomic promoter. expression of aednv vps from te/3'2j/vp was confirmed by northern analysis of rna from infected c6/36 (aedes albopict ... | 1999 | 10208920 |
| transduction of aedes aegypti mosquitoes with vectors derived from aedes densovirus. | aedes densovirus (aednv)-based constructs that express green fluorescent protein (gfp) from either the p7 or the p61 promoter were made. the construct in which gfp protein was expressed as a fusion protein to the c-terminus of ns1 (ns1-gfp) showed the highest level of gfp expression. this hybrid ns1-gfp protein preserved the biological functions of the parental proteins: it showed gfp fluorescence, it stimulated expression from the virus promoters, and it facilitated rescue and replication of th ... | 1999 | 10208921 |
| a new densovirus isolated from the mosquito culex pipiens (diptera: culicidae). | we have isolated from a laboratory strain of culex pipiens in which abnormal larval mortality occurred a small icosahedral nonenveloped dna virus sharing the main biological and biophysical properties of densoviruses (dnvs). unlike dnvs isolated previously from aedes species, i.e. the aaednv and the aaldnv (afanasiev, b.n., galyov, e. e., buchatsky, l.p., kozlov, y.v., 1991. nucleotide sequence and genomic organization of aedes densonucleosis virus. virology 185, 323-336; boublik, y., jousset, f ... | 2000 | 10773314 |
| characterization of the structural gene promoter of aedes aegypti densovirus. | aedes aegypti densonucleosis virus (aednv) has two promoters that have been shown to be active by reporter gene expression analysis (b. n. afanasiev, y. v. koslov, j. o. carlson, and b. j. beaty, exp. parasitol. 79:322-339, 1994). northern blot analysis of cells infected with aednv revealed two transcripts 1,200 and 3,500 nucleotides in length that are assumed to express the structural protein (vp) gene and nonstructural protein genes, respectively. primer extension was used to map the transcrip ... | 2001 | 11152505 |
| aedes aegypti transducing densovirus pathogenesis and expression in aedes aegypti and anopheles gambiae larvae. | aedes aegypti densovirus (aednv) is a small dna virus that has been developed into an expression and transducing vector for mosquitoes [afanasiev et al. (1994) exp parasitol 79: 322-339; afanasiev et al. (1999) virology 257: 62-72; carlson et al. (2000) insect transgenesis: methods and applications (handler, a.m. & james, a.a., eds), pp. 139-159. crc press, boca raton]. virions carrying a recombinant genome expressing the gfp gene were used to characterize the pathogenesis of the virus in 255 in ... | 2001 | 11881803 |
| comparison of penaeid shrimp and insect parvoviruses suggests that viral transfers may occur between two distantly related arthropod groups. | the dna and putative amino acid sequences of representative insect and shrimp parvoviruses (subfamily densovirinae) were analyzed using computer programs. shrimp viruses included hepatopancreatic parvovirus (hpv) of penaeus monodon (hpvmon) and p. chinensis (hpvchin), spawner-isolated mortality virus from p. monodon (smvmon) and infectious hypodermal and hematopoietic necrosis virus (ihhnv) from p. vannamei. insect viruses included aedes aegypti densovirus (aaednv), aedes albopictus densovirus ( ... | 2002 | 12135792 |
| genetic, biochemical, and structural characterization of a new densovirus isolated from a chronically infected aedes albopictus c6/36 cell line. | we report the isolation, sequencing, biochemical, and structural characterization of a previously undescribed virus in a chronically infected aedes albopictus c6/36 cell line. this virus is identified as a new densovirus under the densovirinae subfamily of the parvoviridae based on its biological and morphologic properties as well as sequence homologies, and is tentatively designated a. albopictus c6/36 cell densovirus (c6/36 dnv). analysis of the 4094 nt of the c6/36 dnv genome revealed that th ... | 2004 | 14972541 |
| densonucleosis virus purification by ion exchange membranes. | preparative chromatography is widely used in the downstream purification of biopharmaceutical products. replacement of resins by membranes as chromatographic supports, overcomes many of the limitations associated with resin-based chromatography such as high-pressure drops, slow processing rates due to pore diffusion and channeling of the feed through the bed. in particular, adsorptive membranes may be ideally suited for virus capture. virus capture is critical in a number of applications. in gen ... | 2004 | 15384051 |
| binding aedes aegypti densonucleosis virus to ion exchange membranes. | experimental and numerical results for binding aedes aegypti densonucleosis virus (aednv) using anion and cation exchange membranes are presented. aednv particles are adsorbed by anion and cation exchange membranes providing the virus particles and membranes are oppositely charged. q membranes which are strongly basic anion exchangers were the most effective. dynamic and static capacities for q membranes were found to be similar. a numerical model is proposed which assumes a log normal pore size ... | 2005 | 16188566 |
| a new densovirus isolated from the mosquito toxorhynchites splendens (wiedemann) (diptera: culicidae). | a new densovirus was isolated and characterized in laboratory strains of toxorhynchites splendens. the virus was detected by polymerase chain reaction (pcr) from mosquitoes reared in our laboratory. pcr fragments from each mosquito were compared by single strand conformation polymorphism (sscp) assay and found to be indistinguishable. thus, it is likely the densoviruses from these mosquitoes contain homologous nucleotide sequences. the pcr fragment corresponding to a 451 bp densovirus structural ... | 2007 | 17539278 |
| isolation and characterization of the full coding sequence of a novel densovirus from the mosquito culex pipiens pallens. | during an investigation of arboviruses in china, a novel densovirus (dnv) was isolated from the adult female culex pipiens pallens. the virus, designated culex pipiens pallens densovirus (cppdnv), caused cytopathic effect in c6/36 cells. the virus particles were icosahedral, non-enveloped and had a mean diameter of 24 nm. the complete coding region of cppdnv was found to be 3335 nt and it contained three open reading frames (orfs). cppdnv shares 82-93 % identical nucleotides with isolates of the ... | 2008 | 18089743 |
| aedes aegypti densonucleosis virus amplifies, spreads, and reduces host populations in laboratory cage studies. | aedes densonucleosis virus (family parcoviridae, genus brevidensovirus, aednv) is a mosquito pathogen that increases aedes aegypti larval mortality and reduces adult life span. we conducted three laboratory population cage trials, each lasting 16-25 wk. we tested two broad hypotheses. first, ae. aegypti raised in containers seeded with 10(8) aednv genome equivalents/ml (geq/ ml), a concentration feasible for field application, increase aednv to concentrations that cause significant adult and lar ... | 2009 | 19645297 |
| a large scale laboratory cage trial of aedes densonucleosis virus (aednv). | aedes aegypti (l.) (diptera: culicidae) the primary vector of dengue viruses (denv1-4), oviposit in and around human dwellings, including sites difficult to locate, making control of this mosquito challenging. we explored the efficacy and sustainability of aedes densonucleosis virus (aednv) as a biocontrol agent for ae. aegypti in and among oviposition sites in large laboratory cages (> 92 m3) as a prelude to field trials. select cages were seeded with aednv in a single oviposition site (ops) wi ... | 2010 | 20496587 |
| a recombinant aedna containing the insect-specific toxin, bmk it1, displayed an increasing pathogenicity on aedes albopictus. | the aedes aegypti densovirus (aednv) has previously shown potential in mosquito control. to improve its efficacy as a biopesticide, the gene for an excitatory insect-specific toxin from buthus martensii karsch (bmk it1) was inserted into the aednv genome and cloned into puca plasmid. the coding sequence for green fluorescent protein was ligated to the c-terminus of the bmk it1 gene as a screening marker. recombinant and helper plasmids were cotransfected into c6/36 cells; wild-type viruses were ... | 2010 | 20810829 |
| development of an efficient recombinant mosquito densovirus-mediated rna interference system and its preliminary application in mosquito control. | the aedes aegypti densovirus (aednv) has potential as a delivery vector for foreign nucleic acids into mosquito cells. in this study, we investigated the ability of plasmids containing recombinant viral transducing genome to induce rna interference (rnai) effects in c6/c36 cells. we then evaluated the efficiency of a recombinant aednv vector to induce rnai in aedes albopictus larvae. we found that the expression of v-atpase was inhibited by up to 90% at 96 h post-transfection in transfected c6/c ... | 2011 | 21698129 |
| development of non-defective recombinant densovirus vectors for microrna delivery in the invasive vector mosquito, aedes albopictus. | we previously reported that mosquito densoviruses (mdvs) are potential vectors for delivering foreign nucleic acids into mosquito cells. however, considering existing expression strategies, recombinant viruses would inevitably become replication-defective viruses and lose their ability for secondary transmission. the packaging limitations of the virion represent a barrier for the development of mdvs for viral paratransgenesis or as high-efficiency bioinsecticides. herein, we report the developme ... | 2016 | 26879823 |
| [identification of microrna-like viral small rnas from aaedv]. | several virus families have been shown to encode micrornas (mirnas), which have roles in the infection and replication of viruses in host cells. these virus-encoded mirnas are identified in double-stranded dna virus (dsdna virus) and in several rna virus families, but not in single-stranded dna virus (ssdna virus). we used a bioinformatics approach based on vmir, mirnafold and maturepred software to predict virus-encoded mirna-like small rnas from the genome of a ssdna virus: aedes aegypti denso ... | 2015 | 26738292 |
| application of post-pcr methods for analysis of mosquito densovirus. | two clades of aedes densovirus, aedes aegypti densovirus and aedes albopictus densovirus, were classified according to the origin of isolation. these two densoviruses were isolated from indigenous mosquitoes and mosquito cell lines, respectively. this group of invertebrate viruses belongs to the subfamily densovirinae of the parvoviridae family and infects only insects. several types of densoviruses have been isolated from mosquitoes especially aedes aegypti and aedes albopictus, which are impor ... | 2014 | 25427347 |
| comparison of mosquito densoviruses: two clades of viruses isolated from indigenous mosquitoes. | we analyzed the phylogenetic tree of densoviruses isolated from indigenous mosquitoes and mosquito cell lines. our findings suggest two distinct clades of densovirus. the viruses in the first clade were isolated from an indigenous mosquito which had the aedes aegypti densovirus (aaednv) as a representative virus. the other clade of viruses was isolated from mosquito indigenous cell line which had the aedes albopictus densovirus (aaldnv) as the representative virus. the origin of the two clades o ... | 2013 | 24050091 |
| use of a recombinant mosquito densovirus as a gene delivery vector for the functional analysis of genes in mosquito larvae. | in vivo microinjection is the most commonly used gene transfer technique for analyzing the gene functions in individual mosquitoes. however, this method requires a more technically demanding operation and involves complicated procedures, especially when used in larvae due to their small size, relatively thin and fragile cuticle, and high mortality, which limit its application. in contrast, viral vectors for gene delivery have been developed to surmount extracellular and intracellular barriers. t ... | 2017 | 29053694 |