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hepatozoonosis in foxes from portugal.hepatozoon, sp. is described for the first time in foxes (vulpes vulpes silacea) in portugal. of 301 foxes examined, 143 (48%) were infected. the gametocyte was the predominant stage of the life cycle and was found in every organ except the bone marrow, where schizonts were the most abundant stage. the morphological similarity of this parasite's gametocytes to hepatozoon canis is emphasized.19883373641
molecular survey of hepatozoon canis in red foxes (vulpes vulpes) from romania.blood samples of 119 red foxes, originating from 44 hunting grounds of 3 western counties (arad, hunedoara, and timiş) of romania, have been examined for the presence of hepatozoon canis infection using the conventional polymerase chain reaction (pcr) of the fragment of 18s rrna gene. overall, 15 (12.6%) samples were found to be pcr-positive. of the sampled hunting grounds, 29.5% (13/44) were found positive. positive samples were recorded in all screened counties with the prevalence of 14.8% (9/ ...201525764146
autochthonous hepatozoon infection in hunting dogs and foxes from the czech republic.blood samples from 21 red foxes (vulpes vulpes) and 8 hunting dogs from the same locality in the czech republic were examined for presence of hepatozoon canis/hepatozoon sp. the dogs were selected based on their close contact with foxes during fox bolting and because they had not traveled into known endemic areas. using diagnostic pcr amplifying partial 18s rdna fragment, hepatozoon dna was detected in 20 red foxes (95 %) and 4 dogs (50 %). from 8 positive foxes and 2 positive dogs, we obtained ...201627431805
retrospective molecular study on canine hepatozoonosis in slovakia - does infection risk for dogs really exist?this study provides the very first evidence of hepatozoon canis infection in dogs from slovakia, a rhipicephalus sanguineus sensu lato free area. in total, 297 red foxes (vulpes vulpes) and 293 dogs, from three regions of slovakia, were screened for the presence of h. canis using a conventional 18s rrna polymerase chain reaction (pcr). genomic dna of this blood parasite was detected in 51 (17.1%) foxes from all sampling regions in slovakia, whereas the overall prevalence in examined dogs was sig ...201728366618
hepatozoon canis in german red foxes (vulpes vulpes) and their ticks: molecular characterization and the phylogenetic relationship to other hepatozoon spp.in this study, the prevalence of hepatozoon spp. in red foxes (vulpes vulpes) and their ticks from germany, as well as molecular characterizations and phylogenetic relationship to other hepatozoon spp. were investigated. dna extracts of 261 spleen samples and 1,953 ticks were examined for the presence of hepatozoon spp. by a conventional polymerase chain reaction (pcr) targeting the 18s rrna gene. the ticks included four tick species: ixodes ricinus, ixodes canisuga, ixodes hexagonus and dermace ...201424802869
spatial distribution of anaplasma phagocytophilum and hepatozoon canis in red foxes (vulpes vulpes) in hungary.in recent years, ehrlichia canis and hepatozoon canis transmitted by rhipicephalus sanguineus were reported from hungary. the aim of the present study was to reveal the spatial distribution pattern of pathogens transmitted by r. sanguineus in a sentinel species, red fox (vulpes vulpes) in hungary and to analyse the relationship of these patterns with landscape and climate by geographical information systems. fox carcasses, representing 0.5% of the total fox population were randomly selected out ...201526065623
molecular and histopathological detection of hepatozoon canis in red foxes (vulpes vulpes) from portugal.hepatozoon canis is a protozoan tick-borne pathogen of dogs and wild canids. hepatozoon spp. have been reported to infect foxes in different continents and recent studies have mostly used the polymerase chain reaction (pcr) for the detection and characterization of the infecting species. surveying red foxes (vulpes vulpes) may contribute to better understanding the epidemiology of canine vector-borne diseases, including hepatozoonosis caused by h. canis in domestic dogs. the present study invest ...201424655375
fox on the run--molecular surveillance of fox blood and tissue for the occurrence of tick-borne pathogens in austria.the red fox (vulpes vulpes) is a widespread species, harbouring many pathogens relevant for humans and pets. indeed, anaplasma spp., ehrlichia canis and rickettsia spp. among the bacteria and hepatozoon canis as well as babesia sp. among the parasites have been the focus of several studies.201425413694
a molecular survey of vector-borne pathogens in red foxes (vulpes vulpes) from bosnia and herzegovina.red foxes (vulpes vulpes) have recently been recognized as potential reservoirs of several vector-borne pathogens and a source of infection for domestic dogs and humans, mostly due to their close vicinity to urban areas and frequent exposure to different arthropod vectors. the aim of this study was to investigate the presence and distribution of babesia spp., hepatozoon canis, anaplasma spp., bartonella spp., 'candidatus neoehrlichia mikurensis', ehrlichia canis, rickettsia spp. and blood filaro ...201525889961
first molecular evidence of hepatozoon canis infection in red foxes and golden jackals from hungary.recently, hepatozoon canis infection has been detected among shepherd, hunting and stray dogs in the southern part of hungary, which is considered to be free of rhipicephalus sanguineus sensu lato and close to the border with croatia. the aim of this study was to acquire information on the possibility that red foxes and/or golden jackals could play a role in the appearance and spread of h. canis in hungary.201424985073
wildlife reservoirs for vector-borne canine, feline and zoonotic infections in austria.austria's mammalian wildlife comprises a large variety of species, acting and interacting in different ways as reservoir and intermediate and definitive hosts for different pathogens that can be transmitted to pets and/or humans. foxes and other wild canids are responsible for maintaining zoonotic agents, e.g. echinococcus multilocularis, as well as pet-relevant pathogens, e.g. hepatozoon canis. together with the canids, and less commonly felids, rodents play a major role as intermediate and par ...201425830102
high prevalence of hepatozoon-infection among shepherd dogs in a region considered to be free of rhipicephalus sanguineus.blood samples and ticks were collected from 100 shepherd dogs, 12 hunting dogs and 14 stray dogs in southern hungary, in order to screen them for the presence of hepatozoon spp. by pcr. out of 126 blood samples, 33 were positive (26%). significantly more shepherd dogs (31%) were infected, than hunting (8%) and stray dogs (7%). three genotypes of hepatozoon canis were identified by sequencing, differing from each other in up to six nucleotides in the amplified portion of their 18s rrna gene. in d ...201323499483
the first report of hepatozoon canis identified in vulpes vulpes and ticks from italy.this is the first report on the presence of hepatozoon canis in vulpes vulpes in italy. during the years 2005 and 2006, a total of 119 foxes were collected and their spleen tissues were screened by microscopy, polymerase chain reaction, and sequencing. in the same area, 290 ticks were picked off from dogs or collected from the environment. microscopy detected inclusion bodies regarded as belonging to the genus hepatozoon in four samples, whereas molecular diagnostics evidenced 16 foxes (13.4%) a ...201020420538
hepatozoon canis infection in slovakia: imported or autochthonous?tissue samples from nine red foxes (four samples of striated muscle tissue and five samples of heart tissue) that originated from the michalovce district (slovakia), an area with endemic occurrence of canine babesiosis were examined by pcr method using primers amplifying a fragment of the 18s rrna spanning the v4 region of babesia and theileria. an unexpected determination of 450 bp dna fragment of hepatozoon canis was found in four samples. partial sequences of the 18s rrna gene from the h. can ...200717627439
occurrence and diversity of arthropod-transmitted pathogens in red foxes (vulpes vulpes) in western austria, and possible vertical (transplacental) transmission of hepatozoon canis.red fox (vulpes vulpes) is the most abundant wild canid species in austria, and it is a well-known carrier of many pathogens of medical and veterinary concern. the main aim of the present study was to investigate the occurrence and diversity of protozoan, bacterial and filarial parasites transmitted by blood-feeding arthropods in a red fox population in western austria. blood (n = 351) and spleen (n = 506) samples from foxes were examined by pcr and sequencing and the following pathogens were id ...201728835291
molecular detection of theileria annae and hepatozoon canis in foxes (vulpes vulpes) in croatia.an epizootiological field study on tick-borne protozoan infections in foxes (vulpes vulpes) was carried out in different parts of croatia. spleen samples of 191 carcasses of red foxes killed in sanitary hunting, were examined for the presence of hematozoa by polymerase chain reaction (pcr) and subsequent sequencing. the investigation revealed four species of hematozoa in 57 foxes (30%), namely theileria annae, theileria sp. 3182/05 and hepatozoon canis. t. annae was found in 10 foxes (5%), theil ...201020646832
a serosurvey of hepatozoon canis and ehrlichia canis antibodies in wild red foxes (vulpes vulpes) from israel.a seroepidemiological survey was conducted to investigate the prevalence of antibodies reactive with ehrlichia canis and hepatozoon canis antigens in free-ranging red foxes (vulpes vulpes) in israel. of 84 fox sera assayed, 36% were seropositive for e. canis by the indirect fluorescent antibody (ifa) test and 24% were positive for h. canis using an enzyme-linked immunosrbent assay (elisa). canine ehrlichiosis and hepatozoonosis appear to be endemic in the wild red fox populations in israel, and ...200415036573
molecular studies on babesia, theileria and hepatozoon in southern europe. part i. epizootiological aspects.molecular epizootiology of piroplasmids (babesia spp., theileria spp.) and hepatozoon canis was studied in mammals from southern europe (mainly from spain, but also from portugal and france). partial amplification and sequencing of the 18s rrna gene was used for molecular diagnosis. in some particular cases (b. ovis and b. bovis) the complete 18s rrna gene was sequenced. blood samples were taken from domestic animals showing clinical symptoms: 10 dogs, 10 horses, 10 cows, 9 sheep and 1 goat. in ...200312719133
first report of angiostrongylus vasorum and hepatozoon from a red fox (vulpes vulpes) from west virginia, usa.angiostrongylus vasorum was identified in the lungs of a red fox (vulpes vulpes) from west virginia, united states (us), indicating a new geographical location for this metastrongylid nematode. the fox was euthanized and submitted for necropsy after displaying erratic behavior. we did not detect rabies virus or canine distemper virus from the fox. we observed bronchopneumonia associated with a. vasorum infection disseminated in both lungs. in addition, protozoal meronts were observed in the live ...201424412356
molecular detection of tick-borne pathogens in wild red foxes (vulpes vulpes) from central italy.spleen samples from 153 red foxes, shot during regular hunting season in the province of pisa (central italy), were examined to detect dna of anaplasma phagocytophilum, ehrlichia canis, coxiella burnetii, francisella tularensis, hepatozoon canis and babesia sp./theileria sp. dna of vector-borne pathogens was detected in 120 (78.43%; 95% ci: 71.06-84.66%) foxes. specifically, 75 (49%; 95% ci: 40.86-57.22%) animals scored pcr-positive per h. canis, 68 (44.44%; 95% ci: 36.42-52.69%) for e. canis, 3 ...201728502642
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