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nucleotide sequence analysis of a c1 gene fragment of psittacine beak and feather disease virus amplified by real-time polymerase chain reaction indicates a possible existence of genotypes.to investigate sequence diversity of psittacine beak and feather disease virus, samples collected from 31 psittacine species with or without clinical signs were tested for the presence of the viral genome. a real-time polymerase chain reaction was developed amplifying a 202 base pair fragment of the region encoding the capsid protein c1 and detecting 100 to 1000 genome equivalents. the nucleotide sequences of the polymerase chain reaction products showed 84.1 to 100% identity with no consistent ...200414681067
analysis of the beak and feather disease viral genome indicates the existence of several genotypes which have a complex psittacine host specificity.a study was made of the phylogenetic relationships between fifteen complete nucleotide sequences as well as 43 nucleotide sequences of the putative coat protein gene of different strains belonging to the virus species beak and feather disease virus obtained from 39 individuals of 16 psittacine species. the species included among others, cockatoos ( cacatuini), african grey parrots ( psittacus erithacus) and peach-faced lovebirds ( agapornis roseicollis), which were infected at different geograph ...200415290360
beak and feather disease virus haemagglutinating activity using erythrocytes from african grey parrots and brown-headed parrots.psittacine beak and feather disease (pbfd) is a common viral disease of wild and captive psittacine birds characterized by symmetric feather loss and beak deformities. the causative agent, beak and feather disease virus (bfdv), is a small, circular single-stranded dna virus that belongs to the genus circovirus. bfdv can be detected by pcr or the use of haemagglutination (ha) and haemagglutination inhibition (hi) assays that detect antigen and antibodies respectively. erythrocytes from a limited ...200516300196
phylogenetic analysis of six isolates of beak and feather disease virus from african grey parrots in portugal.beak and feather disease virus (bfdv), a member of the genus circovirus, was detected in six dead african grey parrots (psittacus erithacus) in portugal. the complete nucleotide sequences of these six bfdvs (pt05, pt08, pt08-2, pt08-3, pt09, and pt09-2) were determined and analyzed. the seven open reading frames (orfs) described for other bfdvs were detected in all strains, except for pt05 and pt08, in which orfs 4 and 7 are absent. bayesian inference of phylogeny based on complete genomes of bf ...201020945789
incidence and detection of beak and feather disease virus in psittacine birds in the uae.beak and feather disease is caused by circovirus, which affects actively growing beak and feather cells of avian species. the disease affects mainly young birds while older birds may overcome the disease with few lasting effects. due to lack of treatment, the only way to control the disease is through hygiene and early diagnosis. as a diagnostic tool, we have established a taqman probe based real-time pcr assay to detect the presence of the viral genome in psittacine birds in uae and reported th ...201627077045
molecular analysis and associated pathology of beak and feather disease virus isolated in italy from young congo african grey parrots (psittacus erithacus) with an "atypical peracute form" of the disease.this study is the first report on the genetic and pathogenic characterization of beak and feather disease virus (bfdv) occurring in italy. twenty bfdv strains isolated in italy from juvenile congo african grey parrots (psittacus erithacus) were investigated. seventeen strains showed an "atypical peracute form" (apf) of the disease, and three a chronic form (cf). the birds with apf had been weaned, were independent as far as food and protection were concerned and apparently were without lesions. ...201424968067
relatedness between host species and genotype of beak and feather disease virus suggesting possible interspecies cross infection during bird trade.beak and feather disease virus (bfdv) is a causative agent of psittacine beak and feather disease (pbfd), which shows a characteristic feather disorder in psittacine birds. in the present study, the subclinical infection rate of pbfd in imported and domestically bred psittacine birds was investigated by polymerase chain reaction. as a result, 126 of 402 birds (31.3%) were found to be bfdv positive. the dna sequences of the part of open reading frame (orf) c1 were determined for 16 bfdv-positive ...201323149463
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