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chlamydia pecorum--the fourth species of genus chlamydia. 19938231966
analyses of the genomes of chlamydial isolates from ruminants and pigs support the adoption of the new species chlamydia pecorum.analysis of the genomic dnas of chlamydial isolates from sheep, cattle, and pigs was performed by southern blot hybridization and by restriction endonuclease (re) profiling of dna amplified by pcr. the hybridization probes were derived from whole genomic dna, the major outer membrane protein (momp) gene, the 16s rrna gene, and an avian chlamydia psittaci isolate plasmid. the pcr analysis used targets in the momp gene, the 16s rrna gene, and the 60-kda cysteine-rich protein gene. together, the re ...19968573503
genomic relatedness of chlamydia isolates determined by amplified fragment length polymorphism analysis.the genomic relatedness of 19 chlamydia pneumoniae isolates (17 from respiratory origin and 2 from atherosclerotic origin), 21 chlamydia trachomatis isolates (all serovars from the human biovar, an isolate from the mouse biovar, and a porcine isolate), 6 chlamydia psittaci isolates (5 avian isolates and 1 feline isolate), and 1 chlamydia pecorum isolate was studied by analyzing genomic amplified fragment length polymorphism (aflp) fingerprints. the aflp procedure was adapted from a previously de ...199910419941
growth characteristics of porcine chlamydial strains in different cell culture systems and comparison with ovine and avian chlamydial strains.porcine chlamydiaceae were cultivated under various culture conditions and we compared their growth characteristics with those of ruminant and avian strains. the combination of centrifugation assisted cell culture infection and cycloheximide treatment of vero cell coverslip cultures provided the highest inclusion numbers with all chlamydial strains. interestingly, the use of iscove's modified dulbecco's medium instead of eagle's minimal essential medium significantly increased chlamydia suis inc ...200415158211
recombinant major outer membrane protein (momp) of chlamydophila abortus, chlamydophila pecorum, and chlamydia suis as antigens to distinguish chlamydial species-specific antibodies in animal sera.recombinant major outer membrane proteins (rmomp) of chlamydophila (ch.) abortus, ch. pecorum, and chlamydia (c.) suis were used as antigens to distinguish chlamydial species-specific antibodies in (i) immune sera from six rabbits and three pigs raised against native purified elementary bodies, (ii) serum samples from 25 sows vaccinated with ch. abortus, and (iii) 40 serum samples from four heifers experimentally infected with ch. abortus. all post-exposition sera contained chlamydial antibodies ...200415381270
prevalence of chlamydiae in boars and semen used for artificial insemination.although there are indications for venereal transmission of chlamydiae in pigs, direct diagnostic evidence on the presence of these bacteria in boars and boar semen in particular is still incomplete. we investigated boars from two studs (a, b) in semen (a: n = 174; b: n = 100) and faeces (a: n = 174; b: n = 24) for chlamydiae using ompa-pcr and partial ompa gene sequencing. additionally, blood serum was examined for chlamydial antibodies using an indirect elisa (a: n = 171; b: n = 62). chlamydia ...200616280160
identification and characterisation of coding tandem repeat variants in inca gene of chlamydophila pecorum.bacteria of the family chlamydiaceae are obligate intracellular pathogens of human and animals. chlamydophila pecorum is associated with different pathological conditions in ruminants, swine and koala. to characterize a coding tandem repeat (ctr) identified at the 3' end of inca gene of c. pecorum, 51 strains of different chlamydial species were examined. the ctr were observed in 18 of 18 tested c. pecorum isolates including symptomatic and asymptomatic animals from diverse geographical origins. ...200818651990
preliminary phylogenetic identification of virulent chlamydophila pecorum strains.chlamydophila pecorum is an obligate intracellular bacterium associated with different pathological conditions in ruminants, swine and koala, which is also found in the intestine of asymptomatic animals. a multi-virulence locus sequence typing (mvlst) system was developed using 19 c. pecorum strains (8 pathogenic and 11 non-pathogenic intestinal strains) isolated from ruminants of different geographical origins. to evaluate the ability of mvlst to distinguish the pathogenic from the non-pathogen ...200818707024
recombinant 35-kda inclusion membrane protein inca as a candidate antigen for serodiagnosis of chlamydophila pecorum.chlamydophila pecorum strains are commonly found in the intestine and vaginal mucus of asymptomatic ruminants and may therefore induce a positive serological response when the animals are tested for c. abortus. they have also been associated with different pathological diseases in ruminants, swine and koala. the aim of this study was to identify specific c. pecorum immunodominant antigens which could be used in elisa tests allowing to distinguish between animals infected with c. pecorum and thos ...201019969431
recent advances in the understanding of chlamydophila pecorum infections, sixteen years after it was named as the fourth species of the chlamydiaceae family.chlamydophila pecorum found in the intestine and vaginal mucus of asymptomatic ruminants has also been associated with different pathological conditions in ruminants, swine and koalas. some endangered species such as water buffalos and bandicoots have also been found to be infected by c. pecorum. the persistence of c. pecorum strains in the intestine and vaginal mucus of ruminants could cause long-term sub-clinical infection affecting the animal's health. c. pecorum strains present many genetic ...201019995513
chlamydiaceae infections in pig.abstract: chlamydiaceae are gram-negative obligate intracellular bacteria. they are responsible for a broad range of diseases in animals and humans. in pigs, chlamydia suis, chlamydia abortus, chlamydia pecorum and chlamydia psittaci have been isolated. chlamydiaceae infections in pigs are associated with different pathologies such as conjunctivitis, pneumonia, pericarditis, polyarthritis, polyserositis, pseudo-membranous or necrotizing enteritis, periparturient dysgalactiae syndrome, vaginal di ...201121314912
seroepidemiologic survey for chlamydia suis in wild boar (sus scrofa) populations in italy.we used serology to estimate the prevalence of exposure to chlamydiae in italian populations of wild boars (sus scrofa). sera from 173 hunter-killed wild boars harvested during the 2006-2009 hunting seasons in three italian regions were tested for antibodies to chlamydia suis, chlamydophila pecorum, chlamydophila abortus, and chlamydophila psittaci by the microimmunofluorescence test. antibody titers to chlamydiae ôëñ 1:32 were detected in 110 of the 173 samples tested (63.6%). specific reactivi ...201121719838
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