| a new species of leishmania isolated from the great gerbil rhombomys opimus. | leishmania turanica n.sp., found infecting the desert rodent rhombomys opimus in the southern territories of the ussr and the mongolian people's republic, is described. this parasite exists sympatrically with l. major and l. gerbilli in r. opimus and is the predominant species. a total of 284 isolates of l. turanica from r. opimus, 3 from naturally infected phlebotomus andrejevi and 1 from p. papatasi were characterized and found to be clearly distinguishable on isoenzyme and nuclear dna charact ... | 1990 | 2092289 |
| [leishmaniasis in karamay. xiv. identification of promastigote isolates from naturally infected phlebotomus major wui]. | phlebotomus major wui, an anthropophilic species, was predominant among sandflies in karamay, xinjiang of china. the females of this species collected in the wild and houses were detected to be infected with promastigotes. three isolates of promastigotes obtained from p. major wui were inoculated intraperitoneally and subcutaneously into normal hamsters or balb/c mice which developed visceral leishmaniasis later. the mean size of amastigotes in hamster viscera (smear observation) artificially in ... | 1994 | 7720197 |
| discovery and study of leishmania turanica for the first time in china. | reported are the results of multidisciplinary studies on leishmania turanica, which was isolated from the auricular tissues of naturally infected great gerbils in xinjiang, china. discussed are the biology of the parasite, its molecular biology, its pathogenicity in rodents and humans and its vectors. this was the first time that l. turanica had been reported in china. l. turanica is highly pathogenic in balb/c mice, with the resulting systemic infection being lethal, and it causes dermal lesion ... | 1995 | 8846493 |
| dynamics of leishmania infection rates in rhombomys opimus (rodentia: gerbillinae) population of an endemic focus of zoonotic cutaneous leishmaniasis in iran. | zoonotic cutaneous leishmaniasis (zcl) due to leishmania major is a great public health problem in the old world. leishmania major is widely distributed in populations of rodents in arid and savannah regions. in this study, seasonal variation of natural infection with leishmania parasites in rhombomys opimus (rodentia: gerbillinae) population of an endemic focus of zcl in iran was monitored. the study was conducted from october 2007 to october 2008 in the central part of the country. nested poly ... | 2010 | 20390397 |
| leishmania species: detection and identification by nested pcr assay from skin samples of rodent reservoirs. | many rodent species act as reservoir hosts of zoonotic cutaneous leishmaniasis in endemic areas. in the present study a simple and reliable assay based on nested pcr was developed for the detection and identification of leishmania parasites from rodent skin samples. we designed leishmania-specific primers that successfully amplified its regions of leishmania major, leishmania gerbilli and leishmania turanica using nested pcr. out of 95 field collected rhombomys opimus, 21 were positive by micros ... | 2010 | 20566364 |
| molecular detection of leishmania infection due to leishmania major and leishmania turanica in the vectors and reservoir host in iran. | an epidemiological study was carried out on the vectors and reservoirs of cutaneous leishmaniasis in rural areas of damghan district, semnan province, central iran, during 2008-2009. totally, 6110 sand flies were collected using sticky papers and were subjected to molecular methods for detection of leishmania parasite. phlebotomus papatasi scopoli was the common species in outdoor and indoor resting places. polymerase chain reaction technique showed that 24 out of 218 p. papatasi (11%) and 4 out ... | 2011 | 20575646 |
| species discrimination and phylogenetic inference of 17 chinese leishmania isolates based on internal transcribed spacer 1 (its1) sequences. | leishmaniasis is a geographically widespread disease, caused by protozoan flagellates of the genus leishmania. this disease still remains endemic in china, especially in the west and northwest frontier regions. to date, the phylogenetic relationships among chinese leishmania isolates are still unclear, and the possible taxonomic diversity remains to be established. in this study, the its1-5.8s fragments of ten isolates collected from different foci in china were determined. to infer the phylogen ... | 2010 | 20617444 |
| Isolation and detection of Leishmania species among naturally infected Rhombomis opimus, a reservoir host of zoonotic cutaneous leishmaniasis in Turkemen Sahara, North East of Iran. | In Iran, three species of Leishmania have been incriminated as the causative agents of human leishmaniasis, Leishmania (L.) major, Leishmania tropica, and Leishmania infantum.Rhombomis opimus have been incriminated as a principal reservoirs of the parasitic protozoan Leishmania major, the causative agent of rural zoonotic cutaneous leishmaniasis (ZCL) in Iran. Rodents captured and examined to find Leishmania species using conventional methods including direct impression smear and microscopic obs ... | 2011 | 21945269 |
| the development of leishmania turanica in sand flies and competition with l. major. | in central asian foci of zoonotic cutaneous leishmaniases, mixed infections of leishmania turanica and l. major have been found in a reservoir host (the great gerbil, rhombomys opimus) as well as in the sand fly vector phlebotomus papatasi, but hybrids between these two leishmania species have never been reported. in addition, the role of sand fly species other than p. papatasi in l. turanica circulation is not clear. | 2012 | 23031344 |
| identification and phylogenetic relationship of iranian strains of various leishmania species isolated from cutaneous and visceral cases of leishmaniasis based on n-acetylglucosamine-1-phosphate transferase gene. | the identity of iranian leishmania species has been resolved to some extent by some genetic markers. in this study, based on n-acetylglucosamine-1-phosphate transferase (nagt) gene, we further elucidated the identity and phylogeny of the prevalent species in this country. dnas of 121 isolates belonging to cutaneous leishmaniasis (cl) patients, canine visceral leishmaniasis (cvl) cases, and rhombomys opimus rodents were amplified by targeting a partial sequence of nagt gene. all the amplicons wer ... | 2014 | 24911282 |
| novel identification of leishmania major in hemiechinus auritus and molecular detection of this parasite in meriones libycus from an important foci of zoonotic cutaneous leishmaniasis in iran. | one of the well-known foci of zoonotic cutaneous leishmaniasis (zcl) in iran is turkemen sahara, which is located in north eastern iran. zcl is a disease of mammals, and humans can become infected as accidental hosts. many researchers have argued that rhombomys opimus is the only main reservoir host of zcl in this region of the golestan province. no other rodents or mammals are thought to host or have been reported to host leishmania parasites in this region. this research was designed and devel ... | 2016 | 24613133 |
| high infection frequency, low diversity of leishmania major and first detection of leishmania turanica in human in northern iran. | smears of suspected patients infected with zoonotic cutaneous leishmaniasis (zcl) were stained and examined under a light microscopic observation. dna of parasites within human ulcers was extracted directly from their smears. nested pcr was used to amplify a fragment containing the internal transcribed spacers of the ribosomal rna genes (its-rdna) of lesihmania parasites in human from turkemen sahara located in the northeastern part of iran. based on rflp method by digesting bsuri restriction en ... | 2014 | 24530890 |
| natural infection and phylogenetic classification of leishmania spp. infecting rhombomys opimus, a primary reservoir host of zoonotic cutaneous leishmaniasis in northeast iran. | in the northeast and central parts of iran, rhombomys opimus (great gerbil) is the primary reservoir host of zoonotic cutaneous leishmaniasis (zcl). this study used both parasitological and molecular methods to identify leishmania spp. and their different haplotypes that were circulating in the great gerbil populations in zcl foci from northeastern iran. | 2013 | 23868742 |