| porcine encephalomyocarditis virus persists in pig myocardium and infects human myocardial cells. | recent advances toward using pig tissues in human transplantation have made it necessary to determine the risk of transmitting zoonotic viruses from pigs to humans or vice versa. we investigated the suitability of the porcine encephalomyocarditis virus (emcv) model for such studies by determining its ability to persist in pigs, escape detection by routine serological methods, and infect human cells. intraperitoneal inoculation of 5-week-old pigs with emcv-30, a strain isolated from commercial pi ... | 2001 | 11689644 |
| a wild-type porcine encephalomyocarditis virus containing a short poly(c) tract is pathogenic to mice, pigs, and cynomolgus macaques. | previous studies using wild-type encephalomyocarditis virus (emcv) and mengo virus, which have long poly(c) tracts (61 to 146 c's) at the 5' nontranslated region of the genome, and variants of these viruses genetically engineered to truncate or substitute the poly(c) tracts have produced conflicting data on the role of the poly(c) tract in the virulence of these viruses. analysis of the nucleotide sequence of an emcv strain isolated from an aborted swine fetus (emcv 30/87) revealed that the viru ... | 2003 | 12915530 |
| comparison of the pathogenic, antigenic and molecular characteristics of two encephalomyocarditis virus (emcv) isolates from belgium and greece. | the pathogenicity of two porcine encephalomyocarditis virus (emcv) isolates for sows in gestation and young piglets was studied. one virus originated from a case of reproductive failure in pigs in belgium and the other from a case of acute myocarditis in pigs in greece. sows in the mid-gestation period and one- to two-month old piglets were inoculated with each isolate. the molecular relationship between both isolates was studied by determining the nucleotide sequence located across the junction ... | 1997 | 9300541 |
| genomic analysis of two porcine encephalomyocarditis virus strains isolated in china. | two strains of encephalomyocarditis virus (emcv), designated bjc3 and hb1, were isolated from an aborted fetus and the heart tissue of a dead piglet that had pericardial fluid, respectively. the complete genomic sequences of the two viruses were determined and analyzed. the size of the genomes of bjc3 and hb1 were 7746 and 7735 nucleotides, respectively, including poly(a) tails. comparative analysis with the genomic sequences of other emcv strains showed that bjc3 and hb1 shared higher identity ... | 2007 | 17294091 |
| immune responses and expression of the virus-like particle antigen of the porcine encephalomyocarditis virus. | virus-like particles (vlps) are particles that consist of viral capsid proteins and are structurally similar to authentic virus. to express vlps of the porcine encephalomyocarditis virus (emcv) and investigate their efficacy and immuno response in vivo, a plasmid (p12a3c-pci) containing the p12a and 3c genes of the emcv-k3 viral strain was constructed. the vlps of emcv-k3 were successfully assembled in 293ft cells on 3 days after transfection with p12a3c-pci and were identified as particles of a ... | 2010 | 20378136 |
| immunogenicity and safety of virus-like particle of the porcine encephalomyocarditis virus in pig. | abstract: | 2011 | 21492483 |
| isolation, molecular characterization, and phylogenetic analysis of porcine encephalomyocarditis virus strain hb10 in china. | encephalomyocarditis virus (emcv) can cause myocarditis, respiratory failure, reproductive failure, and mortality in pregnant sows, fetuses, and ablactating piglets. diseases caused by emcv currently affect the swine industry worldwide. a virus was isolated from organs of dead piglets that presented with acute myocarditis in northern china. the production of a specific cytopathic effect on susceptible cells and the results of hemagglutination inhibition (hi) assay, pcr, electron microscopy (em), ... | 2012 | 22538207 |
| prevalent human coxsackie b-5 virus infects porcine islet cells primarily using the coxsackie-adenovirus receptor. | we have previously demonstrated that transplanting porcine encephalomyocarditis virus (emcv)-infected porcine islet cells (pics) results in transmission of the virus to recipient mice, which is manifested by acute fatal infection within 5 to 8 days. here, we determined pic susceptibility to a related and highly prevalent human picornavirus, coxsackie b-5 virus (cvb-5). | 2004 | 15479464 |
| transplanting encephalomyocarditis virus-infected porcine islet cells reverses diabetes in recipient mice but also transmits the virus. | previous studies demonstrated that porcine encephalomyocarditis virus (emcv) caused acute and persistent infection in the myocardium, central nervous system, and spleen of non-human primates (cynomolgus macaques); and it productively infected primary human cardiomyocytes, suggesting that the virus may pose a risk in pig-to-human transplantation. recently, transplantation of myocardial and pancreatic tissues from acutely infected pigs transmitted the virus to recipient mice, resulting in acute fa ... | 2004 | 14962278 |
| transmission of porcine encephalomyocarditis virus (emcv) to mice by transplanting emcv-infected pig tissues. | we recently demonstrated that pigs infected with porcine encephalomyocarditis virus (emcv) develop a persistent infection (up to 90 days post-infection (pi)) in the heart and brain that is accompanied by virus-induced pathologic changes, and that emcv productively infects human cardiomyocytes in vitro, suggesting that emcv may pose a risk to humans following transplantation of pig tissues to humans (brewer et al. j virol 2001; 75: 11621-11629). in this report, we demonstrate that intra-abdominal ... | 2003 | 14708524 |