| resistance to bean common mosaic necrosis virus conferred by the bc-1 gene affects systemic spread of the virus in common bean. | bean common mosaic necrosis virus (bcmnv) isolates belong to two pathogroups (pg), pg-iii and pg-vi, which are distinguished in common bean due to the inability of the pg-iii isolates of bcmnv to overcome the two recessive resistance alleles bc-1 and bc-1(2). the biological and molecular basis of this distinction between pg-iii and pg-vi isolates of bcmnv is not known. here, three isolates of bcmnv were typed biologically on a set of 12 bean differentials and molecularly through whole-genome seq ... | 2017 | 28475025 |
| bean common mosaic virus and bean common mosaic necrosis virus: relationships, biology, and prospects for control. | the closely related potyviruses bean common mosaic virus (bcmv) and bean common mosaic necrosis virus (bcmnv) are major constraints on common bean (phaseolus vulgaris) production. crop losses caused by bcmv and bcmnv impact severely not only on commercial scale cultivation of this high-value crop but also on production by smallholder farmers in the developing world, where bean serves as a key source of dietary protein and mineral nutrition. in many parts of the world, progress has been made in c ... | 2015 | 26111585 |
| first complete genome sequence of bean common mosaic necrosis virus from east timor. | we present here the first complete bean common mosaic necrosis virus (bcmnv) genomic sequence isolated from virus-infected common bean (phaseolus vulgaris) in east timor, and compare it with six complete bmcnv genomes from the netherlands, and one each from the united states, tanzania, and an unspecified country. it most resembled the netherlands strain nl-8 genome. | 2016 | 27688343 |
| a quantitative method to screen common bean plants for resistance to bean common mosaic necrosis virus. | abstract a quantitative method to screen common bean (phaseolus vulgaris) plants for resistance to bean common mosaic necrosis virus (bcmnv) is described. four parameters were assessed in developing the quantitative method: symptoms associated with systemic virus movement, plant vigor, virus titer, and plant dry weight. based on these parameters, two rating systems (v and vv rating) were established. plants from 21 recombinant inbred lines (rils) from a sierra (susceptible) x olathe (partially r ... | 2003 | 18944072 |
| nl-3 k strain is a stable and naturally occurring interspecific recombinant derived from bean common mosaic necrosis virus and bean common mosaic virus. | abstract a strain of bean common mosaic necrosis virus (bcmnv) from idaho was identified by enzyme-linked immunosorbent assay using monoclonal antibodies and determined to be similar to the nl-3 d strain (of drifjhout) by reaction of differential bean cultivars. however, this bcmnv strain (designated nl-3 k) caused earlier and more severe symptoms on bean plants representing host groups 0, 4, and 5. the nucleotide sequence encoding the predicted polyprotein of nl-3 k was 9,893 nucleotides (nt) i ... | 2005 | 18943301 |
| genetic characterization of differential reactions among host group 3 common bean cultivars to nl-3 k strain of bean common mosaic necrosis virus. | abstract a previously unrecognized recessive resistance gene (or allele) was identified in three host group (hg) 3 common bean (phaseolus vulgaris) cvs. olathe, victor, and ui 37, based on genetic analysis of plants from five populations screened with the nl-3 k strain of bean common mosaic necrosis virus (bcmnv). the gene (or allele) was associated with resistance to leaf stunting and deformity and reduction in plant height. the gene (or allele) provides similar, but slightly better resistance ... | 2003 | 18943054 |
| genetic and molecular characterization of the i locus of phaseolus vulgaris. | the i locus of the common bean, phaseolus vulgaris, controls the development of four different phenotypes in response to inoculation with bean common mosaic virus, bean common mosaic necrosis virus, several other related potyviruses, and one comovirus. we have generated a high-resolution linkage map around this locus and have aligned it with a physical map constructed with bac clones. these clones were obtained from a library of the cultivar "sprite," which carries the dominant allele at the i l ... | 2006 | 16322513 |
| phenotypic recombination between bean common mosaic and bean common mosaic necrosis potyviruses in vivo. | the us-5 strain of bean common mosaic virus (bcmv) and the nl-8 strain of bean common mosaic necrosis virus (bcmnv) are both seedborne potyviruses in common bean (phaseolus vulgaris l). they have contrasting and highly stable biological characteristics which are genetically controlled. bcmv strain us-5 belongs to pathogenicity group iv. bcmnv strain nl-8 belongs to pathogenicity group iii. the two strains have contrasting serological characteristics: nl-8 is serotype a; us-5 is serotype b. when ... | 2001 | 11448021 |
| a recombinant of bean common mosaic virus induces temperature-insensitive necrosis in an i gene-bearing line of common bean. | the i gene is a single, dominant gene conferring temperature-sensitive resistance to all known strains of bean common mosaic virus (bcmv) in common bean (phaseolus vulgaris). however, the closely related bean common mosaic necrosis virus (bcmnv) induces whole plant necrosis in i-bearing genotypes of common bean, and the presence of additional, recessive genes is required to prevent this severe whole plant necrotic reaction caused by bcmnv. almost all known bcmnv isolates have so far been classif ... | 2014 | 24875385 |
| potyviral resistance derived from cultivars of phaseolus vulgaris carrying bc-3 is associated with the homozygotic presence of a mutated eif4e allele. | eukaryotic translation initiation factors (eifs) play a central role in potyviral infection. accordingly, mutations in the gene encoding eif4e have been identified as a source of recessive resistance in several plant species. in common bean, phaseolus vulgaris, four recessive genes, bc-1, bc-2, bc-3 and bc-u, have been proposed to control resistance to the potyviruses bean common mosaic virus (bcmv) and bean common mosaic necrosis virus. in order to identify molecular entities for these genes, w ... | 2010 | 20447274 |
| genotyping common bean for the potyvirus resistance alleles i and bc-1(2) with a multiplex real-time polymerase chain reaction assay. | abstract a multiplex real-time polymerase chain reaction (pcr) assay was developed to simultaneously genotype plants for the i and bc-1(2) alleles, which condition resistance in beans to bean common mosaic virus and bean common mosaic necrosis virus. a segregating f(2) population was derived from the cross between pinto bean breeding line p94207-189a (bc-1 bc-1 i i) and olathe (bc-12 bc-1(2) i i). real-time pcr assays were developed that were specific for each allele, and a multiplex pcr reactio ... | 2005 | 18943314 |
| determination of seed-born percentages of bean common mosaic necrosis virus (bcmnv) in three genotypes of phaseolus vulgaris. | bean common mosaic necrosis virus (bcmnv) is one of the most damaging viruses of bean that naturally transmitted by aphid in non persistent manner and through the seeds. bcmnv belongs to the genus potyvirus and the family potyviridae. during the growing season of 2004, bean leaf samples with viral symptoms were collected from tehran province, karaj region. das-elisa by using bcmnv polyclonal antiserum (as-0239, prepared in dsmz, germany) was conducted and samples with viral infection were distin ... | 2006 | 17390883 |