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crystallization and preliminary x-ray analysis of the dsdna bacteriophage hk97 mature empty capsid.hk97 is a temperate dsdna bacteriophage of escherichia coli that is structurally similar to phage lambda, with an icosahedral head of triangulation (7) number 7. although the capsids of several large dsdna phages have been studied extensively using a variety of biophysical approaches, no high-resolution structure is available. we have grown crystals of mature but empty bacteriophage hk97 capsids that diffract to at least 3.5 a using synchrotron radiation. the hk97 head ii crystals are the first ...19989527920
crystallographic analysis of the dsdna bacteriophage hk97 mature empty capsid.hk97 is a member of the siphovirus family of dsdna bacteriophages. it is similar in architecture to bacteriophage lambda, the type member of this family, with an icosahedral capsid of triangulation number t = 7. no high-resolution structural information is available for the dsdna phages, and hk97 is the only dsdna bacteriophage capsid to produce crystals which diffract x-rays. at 650 a in diameter, the large size of the particle and resultant large unit cell create crystallographic challenges. t ...199910089306
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