| anti-erythrocyte membrane antibodies detected in sera of dogs naturally infected with babesia gibsoni. | due to the potential for anti-erythrocyte membrane antibodies as possible enhancers of erythrocyte destruction, the presence of serum anti-erythrocyte membrane antibodies in 31 dogs with babesia gibsoni infection admitted to a veterinary hospital was investigated by an enzyme linked immunosorbent assay (elisa) and immunoblotting analyses. this infection resulted in an increase of anti-erythrocyte membrane antibodies in 84% (igg) and 74% (igm) of 31 infected dogs, respectively. this was confirmed ... | 1992 | 1477157 |
| changes in anti-erythrocyte membrane antibody level of dogs experimentally infected with babesia gibsoni. | to account for the conflict between the excessive destruction of erythrocytes and the number of parasitized erythrocytes in dogs infected with babesia gibsoni, we examined the correlation between anti-erythrocyte membrane antibody level (aemal) and the number of erythrocytes (rbc count) in dogs with experimentally induced babesiosis using hematological examination and an enzyme linked immunosorbent assay (elisa). in the infected dogs without splenectomy, more prominent reduction in rbc count acc ... | 1992 | 1477177 |
| [babesiosis of dogs in germany: epidemiologic case analysis]. | between january 1987 and february 1990 babesia infections were detected in 320 dogs in germany by means of microscopical and/or serological methods. it was found, that 316 dogs were infected with babesia canis and 4 animals with babesia gibsoni. of the babesia-canis-positive dogs 184 were abroad up to 4 months before diagnosis, mainly in france, spain and italy, but also in hungary, greece, jugoslavia, portugal, morocco, togo, pakistan, sri lanka, malaysia, turkey, romania, austria and the nethe ... | 1991 | 2068714 |
| babesia gibsoni in a dog. | | 1968 | 4233354 |
| action of certain antiprotozoal compounds against babesia gibsoni in dogs. | | 1973 | 4703508 |
| [comparative studies on the fine structure of babesia gibsoni and babesia canis]. | | 1968 | 4976227 |
| babesia gibsoni: influence of infection on serum enzymes of dogs. | | 1971 | 5157924 |
| treatment of babesia gibsoni infections with phenamidine isethionate. | | 1970 | 5411947 |
| elevated erythrocyte-bound igg value in dogs with clinical babesia gibsoni infection. | erythrocyte (rbc)-bound igg values (igg concentration, ng/ml) were examined in 8 babesia gibsoni-infected and 10 healthy dogs by a competitive enzyme immunoassay. five dogs with clinical b. gibsoni infection, manifesting severe anemia, were observed to have increased rbc-bound igg values compared to dogs with subclinical b. gibsoni infection and healthy controls, this suggesting that anemia in b. gibsoni infection may be explained in part on the basis of a humoral immunologic mechanism. | 1994 | 7999903 |
| evaluation of the indirect fluorescent antibody test for diagnosis of babesia gibsoni infections in dogs. | we determined the extent of the serologic cross-reactivity in the indirect fluorescent antibody (ifa) test for babesia gibsoni, and the optimal cut-off titer for seropositivity in the test. the lowest titer to b gibsoni detected in a dog with naturally acquired clinical babesiosis was 1,280, and 7 of 12 dogs had titer between 10,240 and 20,480. two experimentally infected normosplenic dogs developed high titer (40,960 to 81,920) to b gibsoni, and the same sera reacted in ifa tests for b canis (t ... | 1993 | 8250380 |
| direct evidence for preferential multiplication of babesia gibsoni in young erythrocytes. | to clarify the effect of the age of host erythrocytes on the multiplication of babesia parasites, b. gibsoni was cultured together with reticulocytes, immature erythrocytes, or mature erythrocytes from dogs. parasitemia reached peak levels (34.1% +/- 15.8%) at cultivation day 8 in immature-erythrocyte culture, whereas the highest parasitemia attained in mature-cell culture was only 3.6% +/- 2.2% at day 5. these results clearly demonstrate that b. gibsoni parasites preferentially invade and multi ... | 1993 | 8327448 |
| babesiosis in washington state: a new species of babesia? | to characterize the etiologic agent (wa1) of the first reported case of babesiosis acquired in washington state. | 1993 | 8328736 |
| isolation of babesia gibsoni piroplasms from infected erythrocytes of dogs. | to eliminate the influence of anti-dog erythrocyte membrane antibodies in babesia gibsoni-infected dogs on the immunological investigations of parasites, an attempt was made to purify b. gibsoni piroplasms from infected erythrocytes by n2 cavitation and centrifugation in a percoll discontinuous density gradient. although electron microscopic examination revealed that the concentrated piroplasms visible as a band at the interface between 30% and 40% percoll solutions were contaminated with a smal ... | 1993 | 8395229 |
| serum hemolytic activity of babesia gibsoni-infected dogs: the difference in the activity between self and nonself red blood cells. | the serum hemolytic activity of babesia gibsoni-infected dogs varied when assayed with nonself red blood cells from different dogs, whereas it did not vary when assayed with red blood cells, irrespective of self or nonself, from a particular dog throughout the experiment. the variety in activity determined with nonself red blood cells was not related to the type of red blood cell by dea, d and j systems. serum hemolytic activity with self red blood cells was different in the course of infection ... | 1993 | 8512998 |
| erythrocyte oxidation in artificial babesia gibsoni infection. | we have already reported that anti-erythrocyte antibody induced by babesia gibsoni infection (aeab) showed higher reactivity against aged and/or oxidized erythrocytes than intact one in vitro (morita et al., 1995). to clarify the meaning of such a binding character of aeab in vivo, changes in erythrocyte oxidation were observed in artificially infected dogs. the ratio of methemoglobin concentration against total hemoglobin concentration (methb%) as the indicator of erythrocyte oxidation was incr ... | 1996 | 8792575 |
| clinical, anatomic, and immunopathologic characterization of babesia gibsoni infection in the domestic dog (canis familiaris). | the pathology associated with acute, chronic, and recrudescent babesia gibsoni infections was characterized in a group of 6 naturally or experimentally infected, spleen-intact and splenectomized dogs. all experimentally infected dogs became acutely parasitemic, lethargic, anemic, thrombocytopenic, and hemoglobinuric. anatomic lesions associated, with the disease included diffuse nonsuppurative periportal and centrilobular hepatitis, multifocal necrotizing arteritis, membranoproliferative glomeru ... | 1997 | 9267413 |
| survey of tick-borne diseases in dogs infested with rhipicephalus sanguineus at a kennel in okayama prefecture, japan. | serological tests were performed to investigate extent of tick-borne diseases in dogs infested with rhipicephalus sanguineus at a kennel in okayama prefecture. three of 22 dogs (13.6%) were positive for ehrlichia canis. two of 19 dogs (10.5%) were positive for rickettsia japonica. three of 22 dogs (13.6%) were positive for babesia gibsoni. none of these animals were positive for coxiella burnetii or hepatozoon canis. a microfilaria was detected in a drop smear of hemolymph from an engorged femal ... | 1998 | 9673953 |
| babesia canis canis, babesia canis vogeli, babesia canis rossi: differentiation of the three subspecies by a restriction fragment length polymorphism analysis on amplified small subunit ribosomal rna genes. | the parasites babesia canis and babesia gibsoni (phylum apicomplexa) are responsible for canine babesiosis throughout the world. babesia canis was previously described as a group of three biologically different subspecies, namely b. canis canis, b. canis vogeli, and b. canis rossi. we report partial sequences of small subunit ribosomal rna gene (ssu-rdna) of each subspecies amplified in vitro with primers derived from a semi-conserved region of the ssu-rdna genes in other babesia species. the po ... | 1999 | 10377990 |
| detection of a new pathogenic babesia microti-like species in dogs. | small babesiae in dogs are generally considered to belong to babesia gibsoni. here we describe the genotypic characterisation of small piroplasms found in the blood of a dog which suffered from clinical babesiosis. pairwise identities as well as distance, parsimony and maximum likelihood analyses of the 18s rdna clearly demonstrated that this isolate was only distantly related to the other canine piroplasms characterised genetically so far, including b. gibsoni. it was more closely related to b. ... | 2000 | 10760414 |
| a genotypically unique babesia gibsoni-like parasite recovered from a dog in oklahoma. | a small babesia gibsoni-like parasite was identified and isolated as the cause of clinical babesiosis in a dog from oklahoma. because this was potentially the first documented case of b. gibsoni infection in oklahoma, further characterization was warranted, and the 18s nuclear small subunit ribosomal rna gene was sequenced. sequence comparison with other piroplasms from dogs showed significant nucleotide sequence differences between this isolate and both b. canis and b. gibsoni. these findings d ... | 2001 | 11318581 |
| fatal babesia gibsoni infection in a dog from spain. | | 2001 | 11467614 |
| development of a polymerase chain reaction method for diagnosing babesia gibsoni infection in dogs. | a pair of oligonucleotide primers were designed according to the nucleotide sequence of the p18 gene of babesia gibsoni (b. gibsoni), nrcpd strain, and were used to detect parasite dna from blood samples of b. gibsoni-infected dogs by polymerase chain reaction (pcr). pcr was specific for b. gibsoni since no amplification was detected with dna from b. canis or normal dog leucocytes. pcr was sensitive enough to detect parasite dna from 2.5 microl of blood samples with a parasitemia of 0.000002%. p ... | 2001 | 11642286 |
| electron microscopic investigations on stages of dog piroplasms cultured in vitro: asian isolates of babesia gibsoni and strains of b. canis from france and hungary. | stages obtained from two asian babesia gibsoni-isolates cultured in vitro were studied by means of transmission electron microscopy and compared to strains of b. canis cultured in vitro. while the developmental stages of the latter preserved their shape in culture, many of the initially small stages of the b. gibsoni strains grew considerably and often looked rather similar to b. canis. | 2002 | 11822735 |
| platelet size, platelet surface-associated igg, and reticulated platelets in dogs with immune-mediated thrombocytopenia. | immune-mediated thrombocytopenia (imt) is a disorder in which bound igg on the surface of platelets results in platelet removal and alterations in mean platelet volume. using flow cytometry, alterations in platelet size, platelet surface-associated igg (psaigg), and numbers of reticulated platelets were determined in 13 dogs with primary imt and 4 dogs with secondary imt induced by experimental infection with babesia gibsoni. effects of sample age on platelet parameters also were determined, usi ... | 2001 | 12024314 |
| molecular characterisation of babesia canis canis and babesia canis vogeli from naturally infected european dogs. | the morphologically small babesia species isolated from naturally infected dogs in europe, japan, and us are described as babesia gibsoni despite the fact that molecular techniques show that they should be assigned to two or three separate taxons. the morphologically large babesia isolated from dogs in europe, africa, and us were generally classified as b. canis until it was proposed to distinguish three related, albeit genetically distinct subspecies of this genus, namely b. canis canis, b. can ... | 2002 | 12079734 |
| molecular cloning and phylogenetic analysis of babesia gibsoni heat shock protein 70. | the babesia gibsoni heat shock protein 70 gene (bghsp70) was cloned by polymerase chain reaction (pcr) and sequenced. the length of the gene was 1938 bp and the predicted polypeptide was 646 amino acids long with a calculated molecular weight of 70,627. the amino acid sequences of bghsp70 from 17 isolates were identical, though there were six types of polymorphisms among the corresponding nucleotide sequences. there was no intron in the bghsp70 gene. phylogenetic analysis of the amino acid seque ... | 2002 | 12446097 |
| transfusion-associated babesia gibsoni infection in a dog. | a 2.5-year-old spayed female german shepherd dog was referred for evaluation of progressive anemia, lethargy, and weight loss. seventeen days earlier, the dog had received a whole blood transfusion to manage hemorrhage after ovariohysterectomy. mild fever, splenomegaly, and thrombocytopenia were also identified. von willebrand disease and babesia gibsoni infection were diagnosed. because of the serologic cross-reactivity of b gibsoni and b canis in the immunofluorescent antibody assay for igg an ... | 2003 | 12685786 |
| cdna cloning of a rab5 homologue in babesia gibsoni. | for studying protein trafficking in babesia-infected erythrocyte, we describe the cloning of a rab5, one of molecular marker for vesicle trafficking in eukaryotic cells, gene homologue in babesia gibsoni (bgrab5). the full-length cdna of bgrab5 is 1,020 bp long with an open reading frame encoding a protein of 220 amino acids. the deduced amino acid sequence of bgrab5 contained the highly conserved gtp-binding consensus sequence and shares about 40% homology with that of rab5 from plasmodium falc ... | 2003 | 12736436 |
| two species of canine babesia in australia: detection and characterization by pcr. | the haemoprotozoan babesia canis has been recognized in australia for many years, and a second, smaller species has recently been discovered. amplification and sequencing of a partial region of the 18s small subunit ribosomal rna (rrna) gene enabled detection and characterization of the large and small canine babesiae of australia for the first time. isolates from northern australia were genetically characterized to be 99% homologous to babesia canis vogeli, confirming previous speculation about ... | 2003 | 12760669 |
| babesia gibsoni: preferential multiplication in reticulocytes is related to the presence of mitochondria and a high concentration of adenosine 5'-triphosphate in the cells. | to determine the cause of the predilection of babesia gibsoni for reticulocytes, the parasites were cultivated with various types of reconstituted erythrocyte ghosts, which were prepared by resealing erythrocyte ghosts together with variously treated erythrocyte lysate, in vitro. the level of parasitemia in the culture with reconstituted reticulocyte ghosts containing untreated reticulocyte lysate was significantly higher than that in the culture with reconstituted normocyte (mature erythrocyte) ... | 2002 | 12856312 |
| epidemiological survey of babesia species in japan performed with specimens from ticks collected from dogs and detection of new babesia dna closely related to babesia odocoilei and babesia divergens dna. | detection and analysis of babesia species from ticks recovered from dogs in japan were attempted by pcr and nucleotide sequence analysis based on the 18s rrna gene, respectively. a total of 1136 ticks were examined for babesia dna by 18s rrna-based pcr and nucleotide sequencing. partial sequences of babesia canis vogeli dna were detected from six ticks in aomori, nara, hiroshima, oita, and okinawa prefectures; and babesia gibsoni asia-1 dna was also detected in four ticks in osaka, hiroshima, mi ... | 2003 | 12904344 |
| serosurvey of antibabesia antibodies in stray dogs and american pit bull terriers and american staffordshire terriers from north carolina. | stray dogs (n=359) and kennel dogs (n=149) from north carolina were tested for evidence of antibabesia antibodies. antibabesia antibodies were detected in 21/359 and 22/149 of the stray and kennel dogs, respectively. a total of 57 dogs from both groups were tested for babesiasis by light microscopy and polymerase chain reaction (pcr). babesia deoxyribonucleic acid (dna) was detected in 3/28 of the stray dogs and 14/29 of the kennel dogs. when babesia dna was detected by pcr, the species-specific ... | 2003 | 14736721 |
| inhibitory effect of antiserum to surface antigen p50 of babesia gibsoni on growth of parasites in severe combined immunodeficiency mice given canine red blood cells. | the inhibitory effect of an antiserum to surface protein p50 of babesia gibsoni on the growth of the parasite was determined with severe combined immunodeficiency mice given canine red blood cells. the antiserum to the recombinant p50 protein significantly inhibited the parasite growth, indicating that p50 might be a useful vaccine candidate. | 2004 | 14977989 |
| the pcr-based detection of babesia gibsoni infection in dogs (german shepherds) reared in south korea. | german-shepherd dogs from the south korean provinces of gangwon and gyunggi were used in a study exploring both the detection of babesia gibsoni infection, by use of a pcr-based assay, and the nucleotide sequences of part of the p18 gene of the parasite. nine (1.8%) of the 501 dogs checked were found pcr-positive. although the prevalence of the infection appeared slightly higher in the male dogs than in the female (2% v. 1.7%), and in the dogs aged <3 years than in the older animals (2.7% v. 1.1 ... | 2004 | 15035725 |
| serodiagnosis of canine babesia gibsoni infection by enzyme-linked immunosorbent assay with recombinant p50 expressed in escherichia coli. | the entire p50 gene encoding a surface protein of babesia gibsoni was cloned into the bacteria expression vector pgex-4t-3 and subsequently expressed in escherichia coli as a glutathione s-transferase fusion protein. the purified recombinant p50 was evaluated in an enzyme-linked immunosorbent assay (elisa) for the serological diagnosis of b. gibsoni infection in dogs. elisa was able to differentiate clearly among b. gibsoni-infected, babesia canis-infected, and uninfected dog sera. the antibody ... | 2004 | 15165064 |
| incidence of canine babesia gibsoni infection and subclinical infection among tosa dogs in aomori prefecture, japan. | to identify the incidence of babesia gibsoni (b. gibsoni) in aomori prefecture, northeastern japan, dogs with acute b. gibsoni infection were investigated at the animal teaching hospital, kitasato university, between april 2002 and march 2003. eighteen dogs with acute b. gibsoni infection were recognized; they were all male dogs of the fighting dog breed tosa. their platelet counts were below normal and their packed cell volumes (pcvs) were at various levels. we collected blood samples from 141 ... | 2004 | 15353837 |
| serodiagnosis of babesia gibsoni infection in dogs by an improved enzyme-linked immunosorbent assay with recombinant truncated p50. | the surface antigen p50 of babesia gibsoni is an important candidate for the development of a diagnostic reagent for canine piroplasmosis. in order to establish an effective diagnostic method for practical use, the gene encoding truncated p50 (p50t) lacking a signal peptide and c-terminal hydrophobic regions were cloned and expressed in escherichia coli as a fusion protein with glutathione s-transferase (gst). more than 90% portion of the gst-p50t was expressed as a soluble form, in contrast wit ... | 2004 | 15644601 |
| clinical observations of babesia gibsoni infection with low parasitaemia confirmed by pcr in dogs. | | 2005 | 15704555 |
| immunization with recombinant surface antigen p50 of babesia gibsoni expressed in insect cells induced parasite growth inhibition in dogs. | this is a report of a vaccine trial directed against babesia gibsoni infection in dogs with the use of the recombinant antigen p50. dogs immunized with p50 showed partial protection manifested as a significantly low level of parasitemia. the results indicated that p50 is a primary vaccine candidate molecule against canine b. gibsoni infection. | 2005 | 15817768 |
| epidemiological survey of babesia gibsoni infection in dogs in eastern japan. | to determine the distribution of babesia gibsoni infection in dogs in the eastern part of japan, an epidemiological survey of dogs suspected of having b. gibsoni infection was attempted using the polymerase chain reaction (pcr) and enzyme-linked immunosorbent assay (elisa). thirty-five of 115 such dogs (30.4%) were positive by pcr and/or elisa. the 35 positive dogs consisted of 28 tosa dogs, 4 american pit bull terriers, and 3 mongrel dogs in aomori, fukushima, ibaraki, gunma, chiba, tokyo, kana ... | 2005 | 15942130 |
| fatal experimental transplacental babesia gibsoni infections in dogs. | a babesia gibsoni infected bitch was mated with an uninfected dog in order to determine whether this parasite could be vertically transmitted. the bitch delivered a litter of four live and one stillborn pup. the four pups died from congenital babesiosis between 14 and 39 days post-birth. babesia gibsoni dna was detected in tissue from all five pups. these results show that vertical transmission occurred by the uterine route and not via the transmammary route. this is the first confirmed report o ... | 2005 | 15979628 |
| development of a practical immunochromatographic test with recombinant p50 for the diagnosis of babesia gibsoni infection in dogs. | an immunochromatographic test (ict), using recombinant truncated p50 (p50t), for the detection of antibodies to babesia gibsoni was developed and evaluated. whereas all sera from specific pathogen-free dogs were clearly negative, all sera from dogs experimentally infected with b. gibsoni were clearly positive in the ict. in addition, the ict detected no cross-reactivity with sera from dogs experimentally infected with closely related parasites, b. canis canis, b. canis vogeli, and b. canis rossi ... | 2005 | 16336730 |
| a review of the small canine piroplasms from california: babesia conradae in the literature. | small piroplasms as a cause of canine babesiosis in southern california were first documented in 1990. initially these piroplasms were considered to be babesia gibsoni, the only small babesia parasite known to infect dogs at that time. in the following decade, the use of molecular analysis made it clear that small canine babesia in fact are comprised of at least three distinct species, and the isolates from dogs in southern california were not b. gibsoni. molecular, antigenic, and morphological ... | 2006 | 16522352 |
| inhibition of na,k-atpase activity reduces babesia gibsoni infection of canine erythrocytes with inherited high k, low na concentrations. | babesia gibsoni multiplies well in canine red blood cells (rbcs) containing high concentrations of potassium (hk), reduced glutathione, and free amino acids as a result of an inherited high na,k-atpase activity, i.e., hk rbcs. to determine the role of na,k-atpase in the multiplication of b. gibsoni, the effect of ouabain on the proliferation of the parasites in hk rbcs was investigated. to determine the direct effect of ouabain on the parasites, the proliferation of the parasites in normal canin ... | 2005 | 16539007 |
| characterization of the babesia gibsoni p18 as a homologue of thrombospondin related adhesive protein. | thrombospondin related adhesive proteins (traps) are well conserved among several apicomplexans. in this study, we reported the identification of the babesia gibsoni p18, designated by our group previously, as a homologue of trap and renamed the p18 as the b. gibsoni trap (bgtrap). the amino acid sequence of bgtrap consists of several typical regions, including a signal peptide, a vonwillebrand factor a domain, a thrombospondin type 1 domain, a transmembrane region, and a cytoplasmic c-terminus. ... | 2006 | 16675041 |
| babesia gibsoni genotype asia in dogs from brazil. | babesia gibsoni was first recognized in india in 1910. analysis of 18s rrna has shown that there are at least three distinct isolates that are morphologically identical. although organisms similar to b. gibsoni have been identified in peripheral blood smears from one dog in brazil, this isolate has not been molecularly characterized. accordingly, we obtained blood samples from 16 dogs with intraerythrocytic inclusion bodies. dna was extracted and amplified with primers that detect a segment of t ... | 2006 | 16765518 |
| prime-boost immunization with dna followed by a recombinant vaccinia virus expressing p50 induced protective immunity against babesia gibsoni infection in dogs. | a heterologous prime-boost immunization regime with priming dna followed by recombinant vaccinia virus expressing relevant antigens has been shown to induce effective immune responses against several infectious pathogens. in this study, we constructed a recombinant plasmid and vaccinia virus, both of which expressed p50 of babesia gibsoni, to investigate the immunogenicity and protective efficacy of a heterologous prime-boost immunization against canine babesiosis. the dogs immunized with the pr ... | 2007 | 17055131 |
| identification of a novel gene encoding a secreted antigen 1 of babesia gibsoni and evaluation of its use in serodiagnosis. | serum from a dog immunized with blood plasma from a b. gibsoni-infected dog, putatively containing secreted antigens, was used to screen a cdna expression library. a novel gene encoding bgsa1 was identified from the isolated clones. the serum raised in mice immunized with the recombinant bgsa1 expressed in escherichia coli could recognize a native parasite protein with a molecular mass of 59 kda. comparing with the previously established elisa with recombinant p50 as antigen, the elisa with reco ... | 2006 | 17123975 |
| pcr-rflp for the detection and differentiation of the canine piroplasm species and its use with filter paper-based technologies. | canine piroplasmosis is an emerging disease worldwide, with multiple species of piroplasm now recognised to infect dogs. a nested polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) assay was developed for the detection and differentiation of each of the piroplasm species currently known to infect dogs on the basis of the 18s ribosomal rna gene. the assay can potentially amplify and discriminate between theileria annae, theileria equi, babesia conradae, babesia gibsoni, ... | 2007 | 17127005 |
| development of an immunochromatographic test with recombinant bgsa1 for the diagnosis of babesia gibsoni infection in dogs. | an immunochromatographic test (ict) using recombinant bgsa1 (rbgsa1) for the detection of antibodies against babesia gibsoni was developed and evaluated. only the serum samples collected from dogs infected with b. gibsoni were positive in the ict, but the serum samples from dogs infected with closely related parasites and from healthy dogs were negative. the specific antibodies could be detected in a dog experimentally infected with b. gibsoni at both the acute and chronic infection stages by th ... | 2007 | 17171565 |
| comparison and phylogenetic analysis of the heat shock protein 70 gene of babesia parasites from dogs. | the heat shock protein 70 (hsp70) genes of babesia gibsoni, b. canis canis, b. canis vogeli, and b. canis rossi isolated from infected dogs were cloned by polymerase chain reaction (pcr) and sequenced. in the nucleotide sequence and the predicted amino acid sequence of the gene, the parasites were very similar to each other. the nucleotide sequences of the hsp70 gene had more variety than those of 18s nuclear subunit ribosomal dna (18s rdna). a phylogenetic analysis of these sequences and compar ... | 2007 | 17289267 |
| babesia gibsoni: detection during experimental infections and after combined atovaquone and azithromycin therapy. | babesia gibsoni is a protozoan parasite of dogs worldwide yet both an effective treatment and a reliable method for detecting subclinical cases of this emerging infection remain elusive. experimental b. gibsoni infections were established in vivo to investigate the efficacy of combined atovaquone and azithromycin drug therapy and to determine the detection limits of a nested-pcr, ifat and microscopy during various stages of infection. while atovaquone and azithromycin produced a reduction in par ... | 2007 | 17543304 |
| first evidence of babesia gibsoni (asian genotype) in dogs in western europe. | canine babesiosis in europe is generally caused by babesia canis canis and babesia canis rossi. here we describe the first two autochthonous cases of babesia gibsoni (asian genotype) infection in germany. two american pit bull terriers showed clinical and hematologic signs consistent with babesiosis. polymerase chain reaction (pcr) analysis of the 18s rdna of blood samples revealed 486 bp fragments. the sequences were 100% identical to each other and 100% identical to babesia gibsoni (asian geno ... | 2007 | 17627433 |
| blood, bull terriers and babesiosis: further evidence for direct transmission of babesia gibsoni in dogs. | this study reports on the epidemiology of babesia gibsoni in american pit bull terriers living in a region of western victoria in southern australia. both american pit bull terriers (n = 100) and other dog breeds (n = 51) were screened for b gibsoni using immunofluorescent antibody testing (ifat) and/or polymerase chain reaction restriction fragment length polymorphism (pcr-rflp). a questionnaire was also completed by each dog owner, ascertaining the husbandry and habits of the dogs sampled. fou ... | 2007 | 17970851 |
| babesia gibsoni: serodiagnosis of infection in dogs by an enzyme-linked immunosorbent assay with recombinant bgtrap. | the thrombospondin-related adhesive protein of babesia gibsoni (bgtrap) is known as an immunodominant antigen and is, therefore, considered as a candidate for the development of a diagnostic reagent for canine babesiosis. the recombinant bgtrap (rbgtrap) expressed in escherichia coli was tested in an enzyme-linked immunosorbent assay (elisa) for detecting antibodies to b. gibsoni in dogs. the elisa with rbgtrap clearly differentiated between b. gibsoni-infected dog sera and specific pathogen-fre ... | 2008 | 18155197 |
| assessment of primers designed for the subspecies-specific discrimination among babesia canis canis, babesia canis vogeli and babesia canis rossi by pcr assay. | canine babesiosis is an infectious disease caused by either babesia gibsoni or babesia canis protozoans. the latter is also classified under three different phylogenetic groups, referred to as subspecies b. canis canis, b. canis vogeli and b. canis rossi. the objective of the present study was to validate and standardize a pcr assay to discriminate the organisms at the subspecies level. first, the reference sequences of the 18s rrna, 5.8s rrna and 28s rrna genes, including the internal transcrib ... | 2008 | 18242863 |
| improvement of dna extraction method for dried blood spots and comparison of four pcr methods for detection of babesia gibsoni (asian genotype) infection in canine blood samples. | to eradicate canine babesiosis in epidemic areas, mass-screening of the infection situation of babesia gibsoni including occult infection is necessary. the development of cost-effective method for storage and transport of blood samples is required. a highly efficient dna extraction procedure from dried blood spots (dbs) onto whatman 3mm filter paper was developed for the diagnosis of b. gibsoni infection in dog by pcr. in 3 extraction methods, chelex-based method in combination with saponin wash ... | 2008 | 18525167 |
| pcr survey of vectorborne pathogens in dogs living in and around barcelona, an area endemic for leishmaniasis. | blood samples from 153 dogs living in and around barcelona were assayed for leishmania infantum and ehrlichia, anaplasma, rickettsia, bartonella, hepatozoon, babesia and theileria species by pcr amplification of dna, and the amplicons obtained were sequenced. the prevalence of the infectious agents was l infantum (29.4 per cent), ehrlichia and anaplasma species (4.0 per cent), hepatozoon canis (3.3 per cent), babesia canis vogeli (2.0 per cent), babesia gibsoni (2.0 per cent), babesia canis cani ... | 2009 | 19168881 |
| diversity of babesia and theileria species in symptomatic and asymptomatic dogs in croatia. | babesiosis, the disease caused by tick-borne hematozoan parasites of the genus babesia, is particularly common in dogs, and is caused by several "large" species of babesia, as well as by an increasing number of "small" species of babesia, some of which appear to be more closely related to members of the genus theileria. in this work, blood samples were collected from 848 randomly selected, asymptomatic dogs and from 81 symptomatic dogs, microscopically positive for babesia, and characterised by ... | 2009 | 19367832 |
| identification of secreted antigen 3 from babesia gibsoni. | a cdna expression library of babesia gibsoni was screened with the serum collected from a dog experimentally infected with b. gibsoni. a novel antigen sharing homology with secreted antigen 1 of b. gibsoni was isolated. the genomic analysis indicated that the bgsa3 gene exists as multicopies in the genome of b. gibsoni. the putative peptide encoded by the bgsa3 gene showed some characteristics of secreted proteins. the serum raised in mice immunized with the recombinant bgsa3 expressed in escher ... | 2009 | 19386799 |
| molecular and immunological characterization of babesia gibsoni and babesia microti heat shock protein-70. | serological immunoscreening was used to identify a gene encoding heat shock protein-70 from babesia gibsoni (bghsp-70) that showed high homology with hsp-70s from other apicomplexan parasites. this gene corresponded to a full-length cdna containing an open reading frame of 1968 bp predicted to result in a 70-kda mature protein consisting of 656 amino acids. analysis of the expression levels of bghsp-70 indicated elevated transcription from cultured parasites incubated at 40 degrees c for 1 h, bu ... | 2009 | 19493212 |
| immunogenicity and growth inhibitory efficacy of the prime-boost immunization regime with dna followed by recombinant vaccinia virus carrying the p29 gene of babesia gibsoni in dogs. | in recent studies, heterologous prime-boost approaches, employing plasmid dna and viral vector pathogen-delivering sequences, have been considered an effective protection strategy for intracellular parasite infections. here, we evaluated the efficacy of such a strategy against the canine babesia gibsoni infection. the dna (pcaggs-p29) and recombinant vaccinia virus (vvp29) both encoding the p29 of b. gibsoni were used in this study. the dogs were immunized 3 times with priming dna and boosted on ... | 2009 | 19712674 |
| detection of babesia gibsoni by reaction of phage display single chain antibodies with p50 proteins. | babesia gibsoni (b. gibsoni) is a tick-borne hemoprotozoan parasite, which causes piroplasmosis in dogs. diagnosis of canine babesiosis is commonly carried out using giemsa-stained thin blood smears. however, at low levels of infection, it is difficult to detect babesia organisms by observation of giemsa-stained thin blood smears. we constructed a monoclonal phage display single chain antibody (scfv) against a b. gibsoni merozoite antigen, p50 protein. intraerythrocytic b. gibsoni organisms are ... | 2009 | 19721374 |
| frequency and therapy monitoring of canine babesia spp. infection by high-resolution melting curve quantitative fret-pcr. | babesia gibsoni and babesia canis are the etiological agents of canine babesiosis, a protozoal hemolytic disease with global significance. canine babesiosis has been diagnosed by microscopic identification of intra-erythrocytic trophozoites in blood smear, and by serological testing. here we developed a quantitative fluorescence resonance energy transfer (fret)-pcr that amplifies a fragment of the babesia spp. 18s rrna gene with high sensitivity and specificity. melting curve analysis differenti ... | 2010 | 19931290 |
| babesiosis caused by a large babesia species in 7 immunocompromised dogs. | a large unnamed babesia species was detected in a dog with lymphoma. it was unknown if this was an underrecognized pathogen. | 2010 | 20002547 |
| a pcr study of piroplasms in 166 dogs and 111 horses in france (march 2006 to march 2008). | positive pcr results for piroplasms were obtained on edta blood samples from 166 dogs and 111 horses between march 2006 and march 2008. the organisms were initially identified using common primers, followed by restriction enzyme profiles to determine the species and types of babesia/theileria. in 27 dogs and eight horses with positive results, the size of the specimen was insufficient to separate species/types. we identified babesia canis canis in 105 of the 166 dogs (63%) and theileria equi in ... | 2010 | 20221639 |
| babesiosis in dogs and cats-expanding parasitological and clinical spectra. | canine babesiosis caused by different babesia species is a protozoal tick-borne disease with worldwide distribution and global significance. historically, babesia infection in dogs was identified based on the morphologic appearance of the parasite in the erythrocyte. all large forms of babesia were designated babesia canis, whereas all small forms of babesia were considered to be babesia gibsoni. however, the development of molecular methods has demonstrated that other babesia species such as ba ... | 2011 | 21571435 |
| expression of truncated babesia gibsoni thrombospondin-related adhesive proteins in escherichia coli and evaluation of their diagnostic potential by enzyme-linked immunosorbent assay. | among the previously established enzyme-linked immunosorbent assays (elisas), an elisa using the full length of a recombinant thrombospondin-related adhesive protein of babesia gibsoni (rbgtrapf) is considered as the most sensitive diagnostic method for the detection of an antibody to b. gibsoni in dogs. however, the expression of rbgtrapf in high concentration is poor and, thus, limits its usefulness as a diagnostic antigen. to improve its expression level, we have truncated bgtrapf into two fr ... | 2011 | 21802417 |
| epidemiological aspects on vector-borne infections in stray and pet dogs from romania and hungary with focus on babesia spp. | canine arthropod-borne infections are of major interest in small animal practice and have been widely investigated in central and western europe. however, only limited epidemiological data are available from south-eastern european countries, although diseases including babesiosis or dirofilariosis are widely recognised as important canine infections in these countries. a steadily increasing number of dogs imported from south-eastern europe into germany require particular attention by small anima ... | 2011 | 21947342 |
| phylogenetic characterization of babesia canis vogeli in dogs in the state of goiás, brazil. | the genus babesia comprises protozoa that cause diseases known as babesiosis. dogs are commonly affected by babesia canis or babesia gibsoni. babesia canis is divided into the subspecies babesia canis canis, babesia canis vogeli and babesia canis rossi. among these, babesia canis vogeli predominates in brazil. the objective of this study was to conduct a phylogenetic analysis on babesia isolates from dogs in goiânia, goiás. blood samples were obtained from 890 dogs presenting clinical signs sugg ... | 2011 | 22166380 |
| molecular investigation of tick-borne pathogens in dogs from luanda, angola. | no molecular data have been available on tick-borne pathogens that infect dogs from angola. the occurrence of agents from the genera anaplasma, babesia, ehrlichia and hepatozoon was assessed in 103 domestic dogs from luanda, by means of the polymerase chain reaction (pcr) and dna sequence analysis. | 2016 | 27160839 |
| canine tick-borne diseases in pet dogs from romania. | tick-borne diseases are of substantial concern worldwide for animals as well as humans. dogs have been a human companion for millennia, and their significant impact on human life renders disease in dogs to be of great concern. tick-borne diseases in dogs represent a substantial diagnostic challenge for veterinarians in that clinical signs are often diffuse and overlapping. in addition, co-infections with two or more pathogens enhance this problem further. molecular methods are useful to disentan ... | 2017 | 28335825 |
| black-backed jackals (canis mesomelas) are natural hosts of babesia rossi, the virulent causative agent of canine babesiosis in sub-saharan africa. | babesia rossi, which is transmitted by haemaphysalis spp. and is highly virulent to domestic dogs, occurs only in sub-saharan africa. since dogs are not native to the region, it has been postulated that the natural host of b. rossi is an indigenous african canid. although various attempts at artificial infection indicated that black-backed jackals (canis mesomelas) could become subclinically infected with b. rossi, data on occurrence of b. rossi in free-ranging jackals was lacking. a long-term b ... | 2017 | 28285591 |
| survey of babesiosis in symptomatic dogs from romania: occurrence of babesia gibsoni associated with breed. | blood samples from 49 symptomatic dogs from 5 western and north-western counties of romania were screened using microscopic examination, polymerase-chain-reaction-restriction-fragment-length-polymorphism procedure (pcr-rflp), and sequence analysis. results of the microscopic evaluation of stained blood smears revealed 45 positive samples with the presence of large and small intraerythrocytic piroplasms in 35 and 10 samples, respectively. babesia canis (35/49, 71.4%) and babesia gibsoni (14/49, 2 ... | 2013 | 23994336 |
| molecular detection of vector-borne pathogens in dogs and cats from qatar. | vector-borne diseases (vbds) have been increasingly reported in dogs and cats worldwide. however, no data are currently available regarding canine and feline vbds in qatar and limited information is available from other persian gulf countries. | 2017 | 28633666 |
| the presence of tick-borne diseases in domestic dogs and cats living on iriomote-jima and tsushima islands. | the iriomote cat and tsushima leopard cat are endangered wildcats in japan and inhabit only iriomote-jima and tsushima islands, respectively. domestic dogs and cats living on iriomote-jima and tsushima islands were surveyed to clarify the interrelationship between wildcats and domestic animals regarding tick-borne disease transmission. pathogen-derived dna in blood samples was detected by polymerase chain reaction. babesia gibsoni was detected in dogs of iriomote-jima, and hepatozoon felis and h ... | 2017 | 28484147 |