Publications

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isolation of a new strain of the flavivirus cell fusing agent virus in a natural mosquito population from puerto rico.the genus flavivirus contains approximately 70 single-stranded, positive-sense rna viruses that are mosquito-borne, tick-borne or have no known vector. two discoveries support previous suggestions of the existence of a large number of unsampled flaviviruses: (i) a new flavivirus, kamiti river virus, was recently isolated from kenyan mosquitoes, and (ii) sequences with high similarity to those of flaviviruses have been found integrated into the genome of aedes mosquitoes, suggesting a past infect ...200616528021
detection and monitoring of mosquito flaviviruses in spain between 2001 and 2005.between the years 2001 and 2005, a total of 72,895 female mosquitoes were trapped during their season of abundance, and analyzed. they were sorted into 4,723 pools belonging to 20 culicidae species from the anopheles, aedes, ochlerotatus, culex, culiseta, coquillettidia, and uranotaenia genera. the aim was to detect arboviral rna directly from mosquito homogenates for the genera alphavirus, flavivirus, and phlebovirus. the study formed part of general arbovirus transmission research in four of t ...200918959502
isolation and characterization of a new insect flavivirus from aedes albopictus and aedes flavopictus mosquitoes in japan.we isolated a new flavivirus from aedes albopictus mosquito and a related species in japan. the virus, designated aedes flavivirus (aefv), only replicated in a mosquito cell line and produced a mild cytopathic effect. the aefv genome was positive-sense, single-stranded rna, 11,064 nucleotides in length and contained a single open reading frame encoding a polyprotein of 3341 amino acids with 5' and 3' untranslated regions (utrs) of 96 and 945 nucleotides, respectively. genetic and phylogenetic an ...200919580982
comparison of dengue virus type 2-specific small rnas from rna interference-competent and -incompetent mosquito cells.the exogenous rna interference (rnai) pathway is an important antiviral defense against arboviruses in mosquitoes, and virus-specific small interfering (si)rnas are key components of this pathway. understanding the biogenesis of sirnas in mosquitoes could have important ramifications in using rnai to control arbovirus transmission. using deep sequencing technology, we characterized dengue virus type 2 (denv2)-specific small rnas produced during infection of aedes aegypti mosquitoes and a. aegypt ...201021049014
detection of mosquito-only flaviviruses in europe.the genus flavivirus, family flaviviridae, includes a number of important arthropod-transmitted human pathogens such as dengue viruses, west nile virus, japanese encephalitis virus and yellow fever virus. in addition, the genus includes flaviviruses without a known vertebrate reservoir, which have been detected only in insects, particularly in mosquitoes, such as cell fusing agent virus, kamiti river virus, culex flavivirus, aedes flavivirus, quang binh virus, nakiwogo virus and calbertado virus ...201222377581
morphological, biochemical, and serological studies on a viral agent (cfa) which replicates in and causes fusion of aedes albopictus (singh) cells. 1976982814
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