| the complete nucleotide sequence of cell fusing agent (cfa): homology between the nonstructural proteins encoded by cfa and the nonstructural proteins encoded by arthropod-borne flaviviruses. | cell fusing agent (cfa) is an rna virus originally isolated from a line of aedes aegypti mosquito cells. although our characterization of the virus many years ago showed that it resembled the flaviviruses, there was no detectable serological cross-reaction with members of the genus flavivirus. furthermore, unlike the well-studied members of the genus flavivirus, cfa did not replicate in any of several vertebrate cell lines tested. we have now determined the nucleotide sequence of the cfa genome. ... | 1992 | 1322586 |
| isolation of a new flavivirus related to cell fusing agent virus (cfav) from field-collected flood-water aedes mosquitoes sampled from a dambo in central kenya. | cell fusing agent virus (cfav) is an rna insect virus that was isolated from a line of aedes aegypti mosquito cells and has been assigned to the family flaviviridae, genus flavivirus. we report here the first isolation of a cfa-like virus from field-collected mosquitoes. mosquito larvae and pupae were sampled from flooded dambos in central province, kenya during the short rain season of 1999. specimens were reared to adults, identified and pooled by species and were tested for the presence of vi ... | 2003 | 12756616 |
| isolation of a new strain of the flavivirus cell fusing agent virus in a natural mosquito population from puerto rico. | the genus flavivirus contains approximately 70 single-stranded, positive-sense rna viruses that are mosquito-borne, tick-borne or have no known vector. two discoveries support previous suggestions of the existence of a large number of unsampled flaviviruses: (i) a new flavivirus, kamiti river virus, was recently isolated from kenyan mosquitoes, and (ii) sequences with high similarity to those of flaviviruses have been found integrated into the genome of aedes mosquitoes, suggesting a past infect ... | 2006 | 16528021 |
| rapid determination of viral rna sequences in mosquitoes collected in the field. | a method for rapid determination of viral rna sequences (rdv) was applied to homogenates of aedes aegypti collected in thailand in an area in which dengue fever (dengue hemorrhagic fever) is endemic, using the mosquito cell line c6/36. nucleic acid sequences of dengue virus type 4 and cell fusing agent virus were detected. this rdv method has the potential to become a standard method for detection of both known and newly emerging, unknown mosquito-borne viruses. | 2007 | 17869351 |
| infection and vertical transmission of kamiti river virus in laboratory bred aedes aegypti mosquitoes. | kamiti river virus (krv) is an insect-only flavivirus that was isolated from field-collected ae. macintoshi mosquitoes in 1999, and is closely related to cell fusing agent virus. both of these viruses belong to the family flaviviridae, which also contains other viruses of medical importance, such as yellow fever virus, west nile virus and dengue. because ae. macintoshi is the only known natural host to krv, the main objective of this study was to establish the possibility that other mosquito hos ... | 2007 | 20337552 |
| comparison of dengue virus type 2-specific small rnas from rna interference-competent and -incompetent mosquito cells. | the exogenous rna interference (rnai) pathway is an important antiviral defense against arboviruses in mosquitoes, and virus-specific small interfering (si)rnas are key components of this pathway. understanding the biogenesis of sirnas in mosquitoes could have important ramifications in using rnai to control arbovirus transmission. using deep sequencing technology, we characterized dengue virus type 2 (denv2)-specific small rnas produced during infection of aedes aegypti mosquitoes and a. aegypt ... | 2010 | 21049014 |
| detection of sequences from a potentially novel strain of cell fusing agent virus in mexican stegomyia (aedes) aegypti mosquitoes. | flaviviruses (fvs) are a very heterogeneous group of viruses that includes viruses capable of infecting insects and/or vertebrates. different human-disease-causing fvs are disseminated by mosquitoes, and therefore, the search for fv in these insects has recently been proposed in order to evaluate their potential transmission in a given community. an entomological survey was carried out in colima (the hyperendemic dengue fever transmission zone in mexico) to collect culicidae in urban and wild ar ... | 2011 | 21409444 |
| genetic and evolutionary analysis of cell-fusing agent virus based on thai strains isolated in 2008 and 2012. | increasing attention is being devoted to ecological and evolutionary relationships between insect-specific flaviviruses and globally important human-pathogenic flaviviruses such as dengue viruses. one such insect flavivirus, cell-fusing agent virus (cfav), remains poorly investigated. in this study, we isolated 13 and 16 cfav strains from aedes aegypti mosquitoes collected in thailand in 2008 and 2012, respectively, and performed genetic and evolutionary analyses based on gene regions encoding t ... | 2013 | 23871775 |
| [detection of flavivirus in mosquitoes (diptera: culicidae) from easter island-chile]. | flaviviruses are arthropod-borne viruses, mainly by mosquitoes of the genera aedes and culex (culicidae) that are detected in tropical and subtropical areas. main flaviviruses of public health importance are: dengue, west nile virus, yellow fever, among others. in continental chile, flaviviruses has not been detected. however, there are indigenous cases of dengue detected in easter island since 2002, as the presence of its vector aedes aegypti. the aim of this study was: to determine diversity o ... | 2015 | 25860055 |
| wolbachia suppresses cell fusing agent virus in mosquito cells. | the genus flavivirus contains a large number of positive-sense ssrna viruses. while some are transmitted by mosquitoes or other arthropods and are pathogenic to humans and animals (e.g. dengue and zika viruses), some are insect-specific and do not replicate in vertebrate cells. these are known as insect-specific flaviviruses (isfs). cell fusing agent virus (cfav) was the first described isf, which was detected in an aedes aegypti cell line, aag2. here, we investigated the effect of wolbachia, a ... | 2016 | 27902358 |
| cell fusing agent virus and dengue virus mutually interact in aedes aegypti cell lines. | the genus flavivirus contains more than 70 single-stranded, positive-sense arthropod-borne rna viruses. some flaviviruses are particularly medically important to humans and other vertebrates including dengue virus (denv), west nile virus, and yellow fever virus. these viruses are transmitted to vertebrates by mosquitoes and other arthropod species. mosquitoes are also infected by insect-specific flaviviruses (isfs) that do not appear to be infective to vertebrates. cell fusing agent virus (cfav) ... | 2017 | 28761113 |
| experimental infection with and maintenance of cell fusing agent virus (flavivirus) in aedes aegypti. | during the past two decades, there has been a dramatic increase in the recognition and characterization of novel insect-specific flaviviruses (isfvs). some of these agents are closely related to important mosquito-borne flavivirus pathogens. results of experimental studies suggest that mosquitoes and mosquito cell cultures infected with some isfvs are refractory to superinfection with related flavivirus pathogens; and it has been proposed that isfvs potentially could be used to alter the vector ... | 2017 | 28719335 |