is222, a new insertion element associated with the genome of pseudomonas aeruginosa.a new insertion element, is222, was identified to be associated with the dna of a mutant strain of the converting pseudomonas aeruginosa bacteriophage d3. the insertion sequence was 1,350 base pairs in size and possessed terminal inverted repeats. the nucleotide sequence contained single cleavage sites for ecori and pvui but none for bamhi, psti, hindiii, smai, or sali. by southern hybridization analysis, no homology was found with genomic dna from p. aeruginosa pat or escherichia coli. genomic ...19863011736
cloning of the early promoters of pseudomonas aeruginosa bacteriophage d3: sequence of the immunity region of d3.the early promoters of bacteriophage d3 of pseudomonas aeruginosa were cloned and physically mapped to the right 25% of the phage genome. the promoters were cloned into promoter selection vector pqf26, and their relative strengths, the direction of transcription, and whether they were directly regulated by repressor were determined. a 3.3-kb fragment of the genome containing the immunity region was sequenced and analyzed (genbank accession number: l22692). the promoter activity associated with t ...19948050993
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