| brucellosis in feral swine in florida. | serums from 95 feral swine trapped in glades county, florida, were tested for brucella antibodies, using the standard tube, card, rivanol, and complement-fixation tests. fifty (53%) of the animals were seropositive to at least 1 test. the standard tube test detected 98% of the serums positive to any of the 4 procedures. the prevalence of brucella antibodies was significantly lower (p less than 0.0005) in feral swine less than 6 months old than in those greater than or equal to 6 months old. the ... | 1978 | 738942 |
| [fertility problems caused by infectious agents pigs in the netherlands(author's transl)]. | the concept of fertility problems is defined in the introduction and their economic importance in pig breeding are pointed out. the infectious causes of the problem are reviewed. infections with l. tarassovi and with aujezky's virus regularly lead to practically endemic abortions; brucella suis and swine-plague infections do so sporadically. the smedi syndrom is considered. the clinical symptoms, diagnosis, epizootiology, and the therapeutical and prophylactic measures of these infections are di ... | 1975 | 1172635 |
| brucella suis biovar 3 infection in a kentucky swine herd. | sows from a large farrow-to-finish operation in western kentucky had late-term abortions. boars and breeding-age sows were tested serologically for brucellosis, and 83 of 125 were classified as reactors. no brucellae were isolated from the tissues of 6 unbred reactor sows, but brucella suis biovar 3 was recovered from 5 aborted fetuses. epidemiological studies failed to determine the source of the infection. | 1989 | 2488645 |
| an evaluation of three serological tests for antibody to brucella suis in pigs. | the rose bengal plate agglutination test (rbt), the complement fixation text (cft) and the tube agglutination test (tat) were applied to serums from 345 feral and 80 domestic pigs sampled at slaughter. at least 2 of the 3 serological tests were applied to each serum. tissues from all pigs were cultured for brucella suis and the degree of culture effort was categorised from 1 to 4 in decreasing order. fifty-eight feral and 35 domestic pigs were culture-positive. a greater proportion of culture-po ... | 1989 | 2712774 |
| selection of a brucella vaccine strain of low residual virulence by chemical mutagenesis. | following incubation of the oral vaccine brucella suis strain 2 with diethyl sulphate (des), a mutant designated strain s105 was selected by screening surviving bacteria for reduced virulence for mice. strain 105 also showed low residual virulence for guinea-pigs and, unlike the parent strain, did not initiate abortion in pregnant sheep and goats after parenteral administration. nevertheless, it was as effective as the parent strain in stimulating protective immunity to brucella melitensis when ... | 1989 | 2795638 |
| swine brucellosis in indonesia. | brucella suis biotype 1 was isolated from 13.1% of the pigs slaughtered in kapuk jakarta, west java and from 15.09% of the pigs slaughtered in surabaya, east java. the prevalence of b. suis by means of the rose bengal plate test, was 22.3% for west java and 14.9% for east java. the rose bengal plate test detected more b. suis infected animals (73% of the infected animals) than did the complement fixation test (41%) and the serum agglutination test (54.5%). the high infection rate is a potential ... | 1988 | 3176296 |
| survey of selected diseases in wild swine in texas. | tissue, fecal, and serum specimens and swabs of nasal turbinates and tracheas were collected from 100 wild swine (sus scrofa) from 10 populations in texas and, along with 24 additional serum specimens, were evaluated for selected swine diseases. swine positive for pseudorabies were detected in 7 populations. brucella suis biovar 1 was isolated from 4 swine from 2 populations, but positive serologic results may indicate a more widespread distribution of the organism. all populations contained swi ... | 1986 | 3505919 |
| orally administrable brucellosis vaccine: brucella suis strain 2 vaccine. | an orally administrable brucellosis vaccine, brucella suis strain 2 vaccine was developed in china. the characteristics and merits of the vaccine are described. it is effective for oral vaccination of sheep, goats, cattle and pigs and has been widely used for prevention of animal brucellosis in china over the past 15 years. about 30-40 million doses of the vaccine are produced every year. | 1986 | 3541425 |
| [isolation of brucella suis from the european hare (lepus auropaeus) in argentinea]. | | 1968 | 4234596 |
| [1st case of human brucellosis due to "brucella suis" observed in france]. | | 1970 | 4313064 |
| [infection by brucella suis in mexico]. | | 1965 | 5838575 |
| [brucella suis infection in mexico]. | | 1965 | 5890759 |
| swine diseases transmissible with artificial insemination. | the transport of fresh and frozen semen to be used for artificial insemination creates a mode of disease transmission between farms. normally, semen contains a number of nonpathogenic bacterial contaminants; however, excessive bacterial contamination can result in infertile matings. contamination with a known pathogen, eg, brucella suis, could initiate a serious outbreak of disease in a recipient herd. methods to minimize bacterial contamination of semen include sanitary collecting and processin ... | 1984 | 6090372 |
| taxonomic studies on some atypical strains of brucella suis. | | 1984 | 6365239 |
| the supposed involvement of dogs carrying brucella suis in the spread of swine brucellosis. | | 1982 | 6821294 |
| differentiation of brucella suis biotype 4 from other biotypes by firenze phage. | | 1982 | 7059778 |
| brucellosis in wild swine: a serologic and bacteriologic survey in the southeastern united states and hawaii. | sera from wild swine from 20 populations in 11 southeastern states and hawaii were tested for brucellosis by the card, tube, plate, buffered plate antigen, complement-fixation, and rivanol tests. twenty-one of 352 (6.0%) swine were considered seropositive, and these animals originated from 7 populations in 5 states: arkansas, florida, georgia, louisiana, and south carolina. cultural examinations for brucella suis also were made from subsamples of the swine tested serologically. brucella suis bio ... | 1982 | 7174444 |
| comparison of serologic tests and bacteriologic culture for detection of brucellosis in swine from naturally infected herds. | to compare results of 6 serologic tests with results of bacteriologic culture for brucella suis in swine. | 1995 | 7591930 |
| [spondylodiscitis cause by brucella suis]. | in a 72-year-old dutchman brucellae suis spondylodiscitis txii-li was diagnosed, complicated by a paraspinal abscess, for which surgical drainage was carried out. the patient failed to recover after the combined antibiotic regimen of doxycycline, rifampicin and trimethoprim-sulfamethoxazole. there was good clinical improvement after a course of doxycycline and streptomycin, however. after a symptom-free period of 9 weeks a relapse occurred. the patient refused treatment and died 6 weeks later at ... | 1994 | 7808527 |
| protein profiles of brucella suis and brucella abortus in isoelectric focusing and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. | the protein profiles of five field isolates of brucella suis and four field isolates of b. abortus were examined. isoelectric focusing of soluble proteins from cell sonicates produced 28 bands common to both species, with a further 10 bands being unique to a species. no intraspecies variation was detected. sodium dodecyl sulfate extracted proteins were resolved by polyacrylamide gel electrophoresis. the proteins were transferred to nitrocellulose and then stained. these techniques allowed the de ... | 1994 | 8203125 |
| immunoblot studies in the differential diagnosis of porcine brucellosis: an immunodominant 62 kda protein is related to the mycobacterial 65 kda heat shock protein (hsp-65). | smooth brucella spp. share certain lipopolysaccharide antigens with other bacteria, resulting in serological cross-reactions which can prevent the definitive diagnosis of brucellosis. to identify other antigens with serodiagnostic potential, immunoblot studies following sodium dodecyl sulphate-polyacrylamide gel electrophoresis were carried out. sera from pigs experimentally infected with brucella suis and naturally infected feral pigs, sera from pigs from a farm with a known history of yersinia ... | 1994 | 8203127 |
| brucellosis: re-emergence and changing epidemiology in queensland. | to define the epidemiological and clinical characteristics of human brucellosis, which is being detected in increasing numbers of patients in queensland. | 1993 | 8336614 |
| serological reactions to brucella species in british pigs. | until and including 1987 diagnostically significant serological titres to swine brucellosis had occurred in the serum agglutination test (sat) or the complement fixation test (cft), ie, > or = 100 iu or > or = 20 icftu, respectively, almost every year since reliable records began, but usually only about 0.05 per cent in the sat and 0.005 per cent in the cft. brucella suis was never isolated by cultural examination. in 1988 the level of cft reactions > or = 20 icftu rose to 0.42 per cent (1.04 pe ... | 1993 | 8517004 |
| protection of balb/c mice against homologous and heterologous species of brucella by rough strain vaccines derived from brucella melitensis and brucella suis biovar 4. | to evaluate stable rough mutants derived from brucella melitensis 16m and b suis 2579 (biovar 4) as vaccines against homologous and heterologous brucella spp in the balb/c mouse model. design, animals, and procedure: rough mutants vtrm1 and vtrs1 were obtained from b melitensis 16m and b suis 2579, respectively, by allelic exchange of rfbu gene encoding mannosyltransferase with a tn5-disrupted rfbu gene. mice were vaccinated with vtrm1 or vtrs1 and challenge exposed 8 weeks later. | 1996 | 8723881 |
| serological and bacteriological study of swine brucellosis. | a serological and bacteriological study was performed with sera taken from 2,228 swine from six states in venezuela. none of the animals were vaccinated against brucellosis, and the prevalence of the disease varied from 5 to 89% on farms located in these states. our studies indicated that the animals could be categorized into four groups depending on the degree of reactivity in serological tests. brucella suis biovar 1 was isolated from the lymph nodes, spleens, and semen samples of seropositive ... | 1997 | 8968931 |
| the public health risks associated with wild and feral swine. | wild swine populations (sus scrofa) are present in many regions of the world. large feral populations in north america and australia are principally derived from introduced domestic pigs. in europe, most wild boar are found in germany and poland. while wild swine are certainly a significant reservoir of infection for domestic swine diseases (for example, african swine fever virus in wild boar in sardinia), these swine generally do not constitute a major public health risk. brucella suis infectio ... | 1997 | 9501373 |
| [brucellosis and aujeszky's disease in a wild boar enclose. case report]. | in wild boars kept in a paddock of approximately 1.7 square miles brucella suis biotype 2 was isolated and aujeszky's disease was diagnosed by serological tests. to clear the paddock, the wild boars were lured by offered food and caught using specially built smaller pens. they were then transported to a location far off their original surroundings and euthanized. of a total of 297 wild boars kept in the paddock, 71% were caught and 28% shot. three wild boars (= 1%) were found trapped. the descri ... | 1999 | 10077814 |
| the elisa for the examination of hare sera for anti-brucella antibodies. | hare brucellosis is caused primarily by brucella suis biovar 2. hares along with wild boars are the natural reservoir of this microorganism. in view of restriction of applicability of traditional serological methods the work aimed to develop the elisa to examine hare sera for the presence of anti-brucella antibodies. lipopolysaccharide (lps) antigen obtained from the strain s19 of brucella abortus and the conjugate of antibodies against rabbit immunoglobulin with horseradish peroxidase were used ... | 1999 | 10099027 |
| validation of the fluorescence polarization assay as a serological test for the presumptive diagnosis of porcine brucellosis. | sera from canadian pigs (brucellosis free, n = 14037) and sera from pigs infected with brucella suis (n = 401) were tested by the buffered antigen plate agglutination test, the complement fixation test, an indirect and a competitive enzyme immunoassay and a fluorescence polarization assay. the results were analysed and assay sensitivity and specificity estimates were calculated. the sensitivity and specificity of the tests were as follows: the buffered antigen plate agglutination test, 77.1 and ... | 1999 | 10510043 |
| evaluation of primary binding assays for presumptive serodiagnosis of swine brucellosis in argentina. | an indirect enzyme-linked immunosorbent assay (ielisa), a competitive elisa (celisa), and a fluorescence polarization assay (fpa) for the presumptive serological diagnosis of swine brucellosis were evaluated using two populations of swine sera: sera from brucellosis-free canadian herds and sera from argentina selected based on positive reactions in the buffered antigen plate agglutination test (bpat) and the 2-mercaptoethanol (2-me) test. in addition, sera from adult swine from which brucella su ... | 2000 | 10973463 |
| visceral abscesses due to brucella suis infection in a retired pig farmer. | a 78-year-old retired pig farmer developed brucellosis and died of liver failure >20 years after her last exposure to infected livestock. this is an exceptionally long incubation period for this infection, which usually presents within weeks of exposure. | 2001 | 11283821 |
| increased prevalence of brucella suis and pseudorabies virus antibodies in adults of an isolated feral swine population in coastal south carolina. | two hundred twenty seven adult (> 8 mo) feral swine (sus scrofa) trapped from april through july 1999 at three locations on a coastal south carolina (usa) peninsula with restricted ingress and egress were tested for brucella suis and pseudorabies virus (prv) antibodies. approximately 44% of the animals tested positive for b. suis antibodies and 61% tested positive for antibodies to prv. previous surveys (1976 and 1992) of feral swine at the same location with similar methods indicated lower sero ... | 2002 | 12238392 |
| brucellosis in central america. | brucellosis is a disease of domestic animals and humans in central america (ca). bovine and swine brucellosis caused by brucella abortus and brucella suis, respectively, have been identified in all ca countries, while ovine and caprine brucellosis caused by brucella melitensis has been detected in guatemala. the prevalence of bovine brucellosis is estimated between 4 and 8%, with higher prevalence in dairy herds, with losses calculated at 25 million us dollars per year. national control programs ... | 2002 | 12414131 |
| brucellosis in the european union and norway at the turn of the twenty-first century. | control and eradication programs of brucellosis in cattle, sheep, goats and pigs have been more or less successfully implemented within the member states (ms) of the european union (eu) and norway after word war ii. as a result, the epidemiological situation of animal brucellosis is extremely diverse among different ms or regions within a ms and among the different animal species. some ms, mainly north european countries, and norway are declared "officially bovine brucellosis free" and/or "offic ... | 2002 | 12414139 |
| how to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing. | collaborative work was financed by the eu to develop and assess new diagnostic tools that can differentiate between bovine brucellosis and bovine infections due to yersinia enterocolitica o:9 either in conjunction with, or as an alternative to, the classical serological, bacteriological or allergic skin tests. sixteen heifers were experimentally infected with brucella abortus biovar 1 (five heifers), brucella suis biovar 2 (two heifers), y. enterocolitica o:9 (six heifers) and y. enterocolitica ... | 2002 | 12414165 |
| brucella vaccines in wildlife. | brucellosis has been known to exist in populations of wildlife since the early part of the 20th century. at the beginning of this century in the us, brucella abortus is a problem in elk and bison in the greater yellowstone area, b. suis is prevalent in millions of feral swine in most of the southern states, and caribou/reindeer in alaska are infected with b. suis biovar 4. brucellosis has been virtually eliminated in domestic livestock in the us after decades of expensive governmental disease pr ... | 2002 | 12414169 |
| brucella suis infection in suckling and weanling pigs. i. | | 1952 | 13022509 |
| brucella suis infection in suckling and weanling pigs. | | 1952 | 13022528 |
| [1st isolation of brucella suis from swine in poland]. | | 1957 | 13469292 |
| [evaluation of 2 hemoculture media for the isolation of brucella spp.]]. | the diagnostic efficiency of two hemoculture media for the detection of different species of brucella strains was evaluated. strains of brucella melitensis, brucella suis, brucella abortus, brucella ovis, and brucella abortus s19 were used. each strain was diluted in phosphate buffer saline (pbs) to obtain a concentration of 10(5) colony forming units/ml (cfu/ml). blood from goats, pigs, cattle, and sheep was mixed with the bacterial suspension to obtain a final concentration minor or equal to 1 ... | 2003 | 14587372 |
| wild boars (sus scrofa) as reservoirs of brucella suis biovar 2 in croatia. | this work presents the results of findings for brucellosis in wild boars and domestic swine in two regions of croatia. in the region of djakovo the blood samples of 211 wild boars were analysed and in 29.4% of the samples serologically positive reactions were established. in the same region the blood samples of 1080 domestic swine on pastures were also analysed and positive serological reactions were established in 12.3%. in the regions around lonjsko polje the blood samples of 53 wild boars wer ... | 2003 | 14680058 |
| from the discovery of the malta fever's agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis. | brucellosis is not a sustainable disease in humans. the source of human infection always resides in domestic or wild animal reservoirs. the routes of infection are multiple: food-borne, occupational or recreational, linked to travel and even to bioterrorism. new brucella strains or species may emerge and existing brucella species adapt to changing social, cultural, travel and agricultural environment. brucella melitensis is the most important zoonotic agent, followed by brucella abortus and bruc ... | 2005 | 15845228 |
| whole-genome analyses of speciation events in pathogenic brucellae. | despite their high dna identity and a proposal to group classical brucella species as biovars of brucella melitensis, the commonly recognized brucella species can be distinguished by distinct biochemical and fatty acid characters, as well as by a marked host range (e.g., brucella suis for swine, b. melitensis for sheep and goats, and brucella abortus for cattle). here we present the genome of b. abortus 2308, the virulent prototype biovar 1 strain, and its comparison to the two other human patho ... | 2005 | 16299333 |
| seroprevalence of brucellosis, tularemia, and yersiniosis in wild boars (sus scrofa) from north-eastern germany. | brucellosis and tularemia are classical zoonotic diseases transmitted from an animal reservoir to humans. both, wildlife and domestic animals, contribute to the spreading of these zoonoses. the surveillance of the animal health status is strictly regulated for domestic animals, whereas systematic disease monitoring in wildlife does not exist. the aim of the present study was to provide data on the prevalence of anti-brucella, anti-francisella and anti-yersinia antibodies in wild boars from north ... | 2005 | 16364020 |
| differentiation between serological responses to brucella suis and yersinia enterocolitica serotype o:9 after natural or experimental infection in pigs. | false-positive serological reactions (fpsr) due to infections with yersinia enterocolitica serotype oratio9 (yeoratio9) are a problem in tests for brucellosis. in the present study, fpsr in classical and novel tests for brucellosis following experimental infections of pigs with yeoratio9 were compared with responses of b. suis biovar 2-inoculated pigs. fpsr were limited to 2-9 weeks post-yeoratio9 inoculation, while b. suis-infected pigs were test-positive throughout the 21-week period of invest ... | 2006 | 16490140 |
| development of a multiplex pcr assay for polymorphism analysis of brucella suis biovars causing brucellosis in swine. | swine brucellosis is caused by the biovars 1, 2 and 3 of brucella suis the identification of which up to now relies on microbiological tests lacking adequate specificity together with time consuming and expensive molecular procedures. based on sequence variation of the omp2b gene, we have developed a four primer set multiplex pcr assay that was tested for polymorphism analysis of b. suis biovars causing brucellosis in swine. the assay exploits the single nucleotide polymorphisms found in omp2b g ... | 2006 | 16530357 |
| parenteral vaccination of domestic pigs with brucella abortus strain rb51. | to determine the immunogenicity and efficacy of brucella abortus strain rb51 (srb51) as a vaccine in domestic pigs. | 2006 | 17014337 |
| cell-mediated immune responses differentiate infections with brucella suis from yersinia enterocolitica serotype o:9 in pigs. | due to almost identical lipopolysaccharide (lps) o-antigens, infections with yersinia enterocolitica serotype o:9 (yeo:9) cause false positive serological reactions (fpsr) in tests for brucella and thus cause problems in national brucella surveillance programs. as lps are strong inducers of antibody responses it was hypothesized that cell-mediated immune responses to non-lps antigens of the two bacteria can be used to separate immune responses to these two biologically very different infections. ... | 2007 | 17270281 |
| prevalence of classical swine fever, aujeszky's disease and brucellosis in a population of wild boar in switzerland. | during two survey rounds of a national surveillance system for infectious diseases in wild boar in switzerland, each lasting four months from november to february, between 2001 and 2003, 1949 blood samples and 62 tissue samples from the spleen and 50 from the reproductive organs were collected from hunted wild boar. the survey was designed so that freedom from infection could be detected with a probability of 95 per cent at a threshold prevalence of less than 1 per cent for classical swine fever ... | 2007 | 17369476 |
| diagnostic characterization of a feral swine herd enzootically infected with brucella. | eighty feral swine were trapped from a herd that had been documented to be seropositive for brucella and which had been used for brucella abortus rb51 vaccine trials on a 7,100-hectare tract of land in south carolina. the animals were euthanized and complete necropsies were performed. samples were taken for histopathology, brucella culture, and brucella serology. brucella was cultured from 62 (77.5%) animals. brucella suis was isolated from 55 animals (68.8%), and all isolates were biovar 1. bru ... | 2007 | 17459850 |
| improving the specificity of immunodiagnosis for porcine brucellosis. | this report describes the use of cell mediated immunity to improve specificity of current diagnosis for brucella suis. diagnosis is problematic due to cross reactions that lead to false positive serological reactions (fpsr) in the standard diagnostic tests. a common cause of this cross reactivity is infection with the organism yersinia enterocolitica o:9. gottingen mini-pigs were experimentally infected with b. suis biovar i field strain or y. enterocolitica serotype o:9 biotype 3. infection was ... | 2008 | 17934790 |
| infection trials in pigs with a human isolate of brucella ( isolate 02/611 'marine mammal type' ). | to determine if pigs could support infection of a human brucella isolate (brucella 02/611) from new zealand, and to study seroconversion to this isolate using a competitive elisa. | 2008 | 18322554 |
| brucella suis identification and biovar typing by real-time pcr. | fast and accurate identification of brucella suis at the biovar level is an important issue for public health laboratories because some of the biovars that infect suidae (boars and pigs) are pathogenic for humans while others are not. since classical biovar typing methods are often time-consuming, hard to standardize and require high-level biosafety containment, methodological improvements are desirable. this article describes new single nucleotide polymorphism (snp) signatures for the rapid ide ... | 2008 | 18499359 |
| brucella suis infection associated with feral swine hunting - three states, 2007-2008. | historically, brucellosis from brucella suis infection occurred among workers in swine slaughterhouses. in 1972, the u.s. department of agriculture national brucellosis eradication program was expanded to cover swine herds. subsequent elimination of brucellosis in commercial swine resulted in a decrease in b. suis-associated illness in humans. currently, swine-associated brucellosis in humans in the united states is predominantly associated with exposure to infected feral swine (i.e., wild boar ... | 2009 | 19521334 |
| pathogen exposure in feral swine populations geographically associated with high densities of transitional swine premises and commercial swine production. | surveys for evidence of exposure to pseudorabies virus (prv), brucella suis, swine influenza virus (siv; human-like h1n1, reassortant type h1n1, h1n2-like h1n1 and h3n2), porcine circovirus 2 (pcv 2), and porcine respiratory and reproductive syndrome virus (prrsv) in feral swine (sus scrofa) were conducted in areas where feral swine were geographically associated with high densities of transitional swine premises in south carolina and high densities of commercial swine production in north caroli ... | 2009 | 19617481 |
| wild boars as sources for infectious diseases in livestock and humans. | wild boars (sus scrofa) are indigenous in many countries in the world. these free-living swine are known reservoirs for a number of viruses, bacteria and parasites that are transmissible to domestic animals and humans. changes of human habitation to suburban areas, increased use of lands for agricultural purposes, increased hunting activities and consumption of wild boar meat have increased the chances of exposure of wild boars to domestic animals and humans. wild boars can act as reservoirs for ... | 2009 | 19687039 |
| epidemiologic survey for brucella suis biovar 2 in a wild boar (sus scrofa) population in northwest italy. | in 2001, antibodies to brucella spp. were detected in the wild boar (sus scrofa) population of regional park in the piedmont, northwest italy. this was the first report of swine brucellosis in italian wildlife, and in response, we conducted a survey on wild boars culled in the park to evaluate the presence and epidemiologic pattern of brucella. in total, 2,267 serum samples and 1,841 tissue samples were collected and tested from 2001 to 2007. differences in antibody and infection prevalence amon ... | 2009 | 19901393 |
| feral pig hunting: a risk factor for human brucellosis in north-west nsw? | a multi-agency investigation followed the notification of four locally acquired human brucellosis cases in north-west nsw. feral pig hunting within a geographically discrete region was identified as the likely exposure with brucella suis the suspected cause. to test whether feral pigs in the region were infected with brucella, serological testing was performed on trapped feral pigs and testicular abscesses from condemned carcasses bound for export were cultured. although no brucella species were ... | 2009 | 20132743 |
| spatial distribution and risk factors of brucellosis in iberian wild ungulates. | the role of wildlife as a brucellosis reservoir for humans and domestic livestock remains to be properly established. the aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for brucellosis transmission in several iberian wild ungulates. | 2010 | 20205703 |
| studies on brucellosis in swine; clinical and histologic features of intracutaneous reactions to fractions of brucella suis. | | 1947 | 20251177 |
| brucellosis in the united states: role and significance of wildlife reservoirs. | regulatory programs for brucellosis in domestic livestock have been active in the u.s. for almost 80 years. wildlife reservoirs of brucellosis include bison (bison bison) and elk (cervus elaphus nelsonii) for brucella abortus whereas brucella suis is the predominant species infecting feral swine. the persistence of brucellosis in wildlife reservoirs poses a risk for reintroduction of brucella into domestic livestock. reducing the prevalence of brucellosis in wildlife reservoirs is anticipated to ... | 2010 | 20362627 |
| brucella suis infection in domestic pigs and wild boar in croatia. | between the years 2000 and 2004, 93,107 sera from 1,997 pig herds in 11 regions of croatia were tested for the presence of antibodies against brucellosis. positive results were observed in 67 herds from seven regions (mean individual prevalence: approximately 1%; herd prevalence: 3.4%). the herds from all but two of the infected farms were reared outdoors and thus almost certainly came into contact with wildlife. from 2003 to 2004, 424 sera, which were randomly collected from hunted wild boar (s ... | 2009 | 20462164 |
| brucellosis in northern australia. | the aim of this study was to define the clinical presentation of brucellosis in northern australia and to assess the long-term impact of brucellosis on individual lives. a retrospective review was conducted to assess 32 patients with brucellosis caused by brucella suis in townsville, australia during 1996-2009. all patients were caucasian males with a mean age of 35 years. the greatest risk factor for brucellosis was feral pig hunting (94%, n = 30). there was one laboratory-acquired case. the mo ... | 2010 | 20889883 |
| gene expression changes in spleens of the wildlife reservoir species, eurasian wild boar (sus scrofa), naturally infected with brucella suis biovar 2. | brucella suis is responsible for swine brucellosis worldwide. of the five different b. suis biovars (bv.), bv. 2 appears restricted to europe where it is frequently isolated from wild boar and hares, can infect pigs and can cause human brucellosis. in this study, the differential gene expression profile was characterized in spleens of eurasian wild boar naturally infected with b. suis bv. 2. of the 20,201 genes analyzed in the microarray, 633 and 1,373 were significantly (fold change > 1.8; p < ... | 2010 | 21115167 |
| over-expression of homologous antigens in a leucine auxotroph of brucella abortus strain rb51 protects mice against a virulent b. suis challenge. | infection by members of the gram-negative bacterial genus brucella causes brucellosis in a variety of mammals. brucellosis in swine remains a challenge, as there is no vaccine in the usa approved for use in swine against brucellosis. here, we developed an improved recombinant brucella abortus vaccine strain rb51 that could afford protection against brucella suis infection by over-expressing genes encoding homologous proteins: l7/l12 ribosomal protein, cu/zn superoxide dismutase [sod] and glycosy ... | 2011 | 21376799 |
| proinflammatory caspase-2-mediated macrophage cell death induced by a rough attenuated brucella suis strain. | brucella spp. are intracellular bacteria that cause an infectious disease called brucellosis in humans and many domestic and wildlife animals. b. suis primarily infects pigs and is pathogenic to humans. the macrophage-brucella interaction is critical for the establishment of a chronic brucella infection. our studies showed that smooth virulent b. suis strain 1330 (s1330) prevented programmed cell death of infected macrophages and rough attenuated b. suis strain vtrs1 (a vaccine candidate) induce ... | 2011 | 21464087 |
| a novel isolation method of brucella species and molecular tracking of brucella suis biovar 2 in domestic and wild animals. | brucella suis biovar 2 is the most common aetiological agent of porcine brucellosis in europe. b. suis biovar 2 is considered to have low zoonotic potential, but is a causative agent of reproductive losses in pigs, and it is thus economically important. the multilocus variable-number of tandem repeats genotyping analysis of 16 loci (mlva-16) has proven to be highly discriminatory and is the most suitable assay for simultaneously identifying b. suis and tracking infections. the aim of this study ... | 2011 | 21474256 |
| epidemiological and clinical aspects of human brucella suis infection in polynesia. | summaryhigh brucellosis seroprevalence rates in domestic swine herds have been reported in wallis and futuna islands and are associated with a significant burden of human infection by brucella suis, a species that is rarely incriminated in human disease. between 2003 and 2010, seven patients had a positive blood culture for b. suis biovar 1, 11 symptomatic patients had a positive rose bengal test (rbt) and a positive serum agglutination test (sat) and three asymptomatic cases were found to be po ... | 2011 | 21733252 |
| Blood, guts and knife cuts: reducing the risk of swine brucellosis in feral pig hunters in north-west New South Wales, Australia. | Humans who have close contact with livestock, wild or feral animals can risk acquiring zoonotic infections such as brucellosis, Q fever, and leptospirosis. Human infection with <em>Brucella suis</em> (swine brucellosis) usually follows occupational or recreational exposure to infected animals. Worldwide, many cases of human infection follow contact with infected feral pigs. In Australia there is a growing market for the export of 'wild boar' and a considerable number of people are involved in fe ... | 2011 | 22182348 |
| Development and evaluation of a selective medium for Brucella suis. | A new selective medium, named LNIV-M, has been developed for isolation of Brucella suis. In this work, we evaluated the growth of B. suis reference and field strains from domestic pigs in different basal media and the susceptibility to different antibiotics contained in the currently used Farrell's and modified Thayer-Martin media. We also determined the efficacy of LNIV-M and its diagnostic performance for isolating B. suis from wild boar tissue samples. A total of 1649 samples from 918 hunter- ... | 2011 | 21968103 |
| Free-ranging wild boar: a disease threat to domestic pigs in Switzerland? | The risk of transmission of pathogens from free-ranging wild boars (Sus scrofa scrofa) to outdoor domestic pigs (S. scrofa domesticus) is of increasing concern in many European countries. We assess this risk, using Switzerland as an example. We estimated 1) the prevalence of important pathogens in wild boars and 2) the risk of interactions between wild boars and outdoor pigs. First, we tested 252 wild boars from selected areas between 2008 and 2010 for infection with Brucella spp. Bacterial prev ... | 2011 | 22102657 |
| complete genome sequence of brucella suis vbi22, isolated from bovine milk. | brucella suis is the causative agent of swine brucellosis and is known to be able to infect several different hosts, including cattle, dogs, and horses, without causing disease symptoms. here we report the complete genome sequence of brucella suis vbi22, which was isolated from raw milk from an infected cow. | 2012 | 22275106 |
| isolation of brucella suis biovar 2 from a wild boar in the abruzzo region of italy. | a female wild boar, aged approximately two years, was found dead by local veterinary services in pianola di roio in l'aquila province situated in the abruzzo region of central italy. the carcass was submitted to the istituto zooprofilattico sperimentale dell'abruzzo e del molise 'g. caporale' in teramo for necropsy. brucella suis biovar 2 was isolated from submandibular lymph nodes. this is the first report of isolation of b. suis in the abruzzo region. several authors agreed in the past on the ... | 2012 | 23277120 |
| unexpected brucella suis biovar 2 infection in a dairy cow, belgium. | | 2013 | 24274041 |
| risk factors for contacts between wild boar and outdoor pigs in switzerland and investigations on potential brucella suis spill-over. | due to the parallel increase of the number of free-ranging wild boar and domestic pigs reared outdoor, the risk that they interact has become higher. contacts with wild boar can be the origin of disease outbreaks in pigs, as it has been documented for brucellosis in some european countries. this study aimed at quantifying the occurrence of contacts between wild boar and outdoor domestic pigs in switzerland, and identifying risk factors for these contacts. furthermore, exposed pigs were tested fo ... | 2012 | 22817843 |
| a serological and bacteriological survey of brucellosis in wild boar (sus scrofa) in belgium. | brucellosis is frequently reported among wild boar populations in europe. the aim of the study was to assess the epidemiological situation in belgium, regarding the steady increase of wild boar populations over the last decades. several serological tests were used and compared with culture and is711 polymerase chain reaction (pcr), to determine the most suitable combination of diagnostic tools for conducting a successful prevalence study in wildlife. | 2012 | 22709889 |
| complete genome sequences of two central european brucella suis bv. 2 haplotype 2c strains isolated from wild boars. | the brucella suis haplotype 2c is commonly isolated from wild boars and domestic pigs across central europe, though it is rarely described in the iberian peninsula. we report here the complete and annotated genome sequences of two haplotype 2c strains isolated from wild boars in the northeast region of spain, above the ebro river. | 2014 | 25013144 |
| complete genome sequences of three iberian brucella suis biovar 2 strains isolated from wild boars. | brucella suis biovar 2 is the most common biovar isolated from wild boars (sus scrofa) associated with transmission to outdoor-reared pigs in europe. we report here the complete and annotated genome sequences of three strains isolated from wild boars in portugal and spain and belonging to the iberian clone (haplotypes 2d and 2e). | 2014 | 24994794 |
| evaluating the risk of pathogen transmission from wild animals to domestic pigs in australia. | wild animals contribute to endemic infection in livestock as well as the introduction, reintroduction and maintenance of pathogens. the source of introduction of endemic diseases to a piggery is often unknown and the extent of wildlife contribution to such local spread is largely unexplored. the aim of the current study was to quantitatively assess the probability of domestic pigs being exposed to different pathogens from wild animals commonly found around commercial piggeries in australia. spec ... | 2016 | 26711303 |
| use of serology and bacterial culture to determine prevalence of brucella spp. in feral swine (sus scrofa) in proximity to a beef cattle herd positive for brucella suis and brucella abortus. | using serology and bacterial culture, we determined the prevalence of brucella spp. and the antibody to brucella spp. in a feral swine (sus scrofa) population in proximity to a cattle herd that was culture positive for brucella abortus and brucella suis in north-central texas, usa. during a prospective cross-sectional quantitative study in april 2005, we collected blood and tissue samples from 40 feral swine within a 30-km radius of the infected herd. serum samples were tested by the rose bengal ... | 2013 | 23568896 |
| the first report of brucella suis biovar 1 isolation in human in turkey. | brucella melitensis and b. abortus are the species generally isolated from human samples in turkey. several studies have also demonstrated the presence of antibodies against b. canis. | 2017 | 26947547 |
| boar hunting and brucellosis caused by brucella suis. | brucellosis remains as a significant public health concern in many areas where the infection persists in domestic hosts (i.e. goats, cattle, and domestic swine) with subsequent risk of transmission to human populations. brucellosis caused by b. suis remains an important threat to human populations in many countries exposed to domestic and feral swine. in the u.s., swine brucellosis has been under control for many years. meanwhile, it is a widespread infection among feral swine, particularly in t ... | 2017 | 28300645 |
| whole-genome mapping reveals a large chromosomal inversion on iberian brucella suis biovar 2 strains. | optical mapping is a technology able to quickly generate high resolution ordered whole-genome restriction maps of bacteria, being a proven approach to search for diversity among bacterial isolates. in this work, optical whole-genome maps were used to compare closely-related brucella suis biovar 2 strains. this biovar is the unique isolated in domestic pigs and wild boars in portugal and spain and most of the strains share specific molecular characteristics establishing an iberian clonal lineage ... | 2016 | 27527786 |
| characterisation of brucella suis isolates from southeast europe by multi-locus variable-number tandem repeat analysis. | porcine brucellosis is a common bacterial zoonosis which can cause significant financial losses. its diverse and often complicated factors have hampered efforts to control disease spread. the aim of the study was to assess the epidemiological situation of porcine brucellosis primarily in croatia and its relationship to genotypes present in other, mostly european countries. one hundred and seven brucella suis strains isolated from swine, hares, cattle, humans, wild hares, a wild boar and a mare o ... | 2015 | 26324171 |
| a serosurvey for brucellosis in wild boar (sus scrofa) in sardinia, italy. | porcine brucellosis is a zoonotic disease caused by brucella suis and hosted by pigs (sus scrofa). both domestic pigs and wild boars are affected. we measured the prevalence of antibody to brucella spp. in wild boars in sardinia, italy. during 1 november 2009 to 31 january 2010, we collected 570 serum samples from legally hunted wild boars and tested them using a commercial competitive enzyme-linked immunosorbent assay. sex and age class of the sampled wild boars were also recorded. thirty-five ... | 2015 | 26267463 |
| first report of brucella suis biovar 2 in a semi free-range pig farm, italy. | this communication describes the isolation of brucella suis (b. suis) biovar 2 in semi‑free‑range pigs located in the province of rome, italy. sera of 28 pigs from a herd with reproductive problems were tested for brucellosis. twenty-five sera (89%) were found positive to rose bengal test (rbt), while 22 (79%) were positive to complement fixation test (cft). two positive pigs were slaughtered, organs were collected and tested for the presence of bacteria. brucella spp. was isolated from the sple ... | 2015 | 26129667 |
| genetic relatedness of brucella suis biovar 2 isolates from hares, wild boars and domestic pigs. | porcine brucellosis generally manifests as disorders in reproductive organs potentially leading to serious losses in the swine industry. brucella suis biovar 2 is endemic in european wild boar (sus scrofa) and hare (lepus europeus, lepus capensis) populations, thus these species may play a significant role in disease spread and serve as potential sources of infection for domestic pigs. the aim of this study was an epidemiologic analysis of porcine brucellosis in hungary and a comparative analysi ... | 2014 | 24962519 |
| identification of brucella suis from feral swine in selected states in the usa. | serologic tests currently available for brucellosis diagnosis detect antibodies to brucella but do not distinguish between species of brucella. although brucella suis is known to circulate within various feral swine (sus scrofa) populations, our objective was to determine the primary species of brucella circulating in feral swine populations in areas of the us with high brucellosis prevalence. we cultured lymph nodes from 183 feral swine. we identified 22 isolates from 21 animals, and all isolat ... | 2014 | 24484477 |
| brucella suis biovar 2 isolations from cattle in poland. | bovine brucellosis is an infectious disease caused by bacteria of the brucella genus, primarily by b. abortus, less frequently by b. melitensis, and occasionally by b. suis. in the european union, brucellosis in cattle has been eradicated in most of the member states, which are recognized as 'officially free from bovine brucellosis'. nevertheless, cattle herds continue to be serologically monitored for the potential re-emergence of the disease. the aim of the presented study was to show the resu ... | 2013 | 24364432 |
| high-density dependence but low impact on selected reproduction parameters of brucella suis biovar 2 in wild boar hunting estates from south-western spain. | porcine brucellosis is a disease caused by brucella suis, which is characterized by reproductive disorders in pigs. the number of cases of swine brucellosis has risen in many european countries, likely because of the presence of a wild reservoir of b. suis in wild boar. this study aimed at evaluating factors that may influence the probability of infection with brucella spp. in wild boar and at assessing the impact of a previous contact with brucella spp. on reproductive parameters of wild boar. ... | 2014 | 23347330 |
| development and evaluation of diagnostic tests for the serological diagnosis of brucellosis in swine. | a competitive enzyme-linked immunosorbent assay (c-elisa), an indirect elisa (i-elisa) and a dissociation-enhanced lanthanide fluorescence immunoassay (delfia) were developed to test for antibodies to brucella suis in pig and wild boar sera. an anti-brucella-lps monoclonal antibody (mab 4b5a) (c-elisa and delfia) and an anti-swine igg monoclonal antibody (mab 10c2g5) (i-elisa) were used for the three assays. the specificity (sp) and sensitivity (se) of the assays gave the following results: se a ... | 2013 | 22718331 |
| a serosurvey for brucella suis, classical swine fever virus, porcine circovirus type 2, and pseudorabies virus in feral swine (sus scrofa) of eastern north carolina. | as feral swine (sus scrofa) populations expand their range and the opportunity for feral swine hunting increases, there is increased potential for disease transmission that may impact humans, domestic swine, and wildlife. from september 2007 to march 2010, in 13 north carolina, usa, counties and at howell woods environmental learning center, we conducted a serosurvey of feral swine for brucella suis, pseudorabies virus (prv), and classical swine fever virus (csfv); the samples obtained at howell ... | 2012 | 22493123 |
| brucella suis biovar 2 infection in humans in france: emerging infection or better recognition? | brucellosis is usually acquired by humans through contact with infected animals or the consumption of raw milk from infected ruminants. brucella suis biovar 2 (bsb2) is mainly encountered in hares and wild boars (sus scrofa), and is known to have very low pathogenicity to humans with only two case reports published in the literature. human cases of brucellosis caused by bsb2 were identified through the national mandatory notification of brucellosis. the identification of the bacterium species an ... | 2017 | 28784192 |