| vaccination of adult sheep with reduced doses of brucella melitensis strain rev.1: safety and serological responses. | a trial was made to test the safety of a brucella melitensis strain rev. 1 vaccine when used in various doses on pregnant sheep and a control group of pregnant goats. it was found that local sheep were more susceptible than goats to the effects of this vaccine, and that the accepted dosage rate of 10+5 viable organisms, as recommended for adult goats, should not be used on pregnant sheep. | 1977 | 906093 |
| evidence of reservior hosts of brucella mellitensis. | serological reactors to brucella melitensis were found among goats and eland in chilanga. the only clinical evidence of disease were abortion among the goats, and circumstances did not allow for isolation of the organism in the laboratory. no gross pathological lesions were observed on post mortem examination of the goats. the public health significance relates to the ease of br. melitensis transmission between reservoir hosts and human beings. | 1976 | 1052104 |
| brucellosis outbreak attributed to ingestion of umpasteurized goat cheese. | in six cases of brucella melitensis infections the common etiologic factor was the ingestion of raw goat cheese. presenting complaints were variable, but a constant feature was persistent fevers that frequently were present for months. evidence of liver cell dysfunction was present in each case, and in two, biopsy disclosed diffuse hepatitis with focal necrosis. the diagnoses were initially based on high titers of brucella agglutinins and later confirmed by positive blood cultures. a good clini ... | 1975 | 1170820 |
| brucellosis linked to mexican cheese. | three cases of brucellosis occurred, two acute cases and one chronic, all of which likely resulted from the ingestion of fresh cheese containing brucella melitensis. a history of patients with illness suggestive of brucellosis should include details about food ingested recently in addition to the usual questions about travel and occupation. as the us domestic-animal disease comes under better control, and travel to mexico, spain, and italy increases, it seems likely that human disease caused by ... | 1975 | 1173155 |
| brucellosis as a world problem. | brucellosis is one of the most widespread and economically the most ravaging of zoonoses. the occurrence of the acute, often incapacitating infection in man caused by brucella melitensis usually coincides with occurrence of the infection in sheep and goats. well-known foci of this infection have been identified in the mediterranean basin, central asia and latin america. recent investigations in parts of africa and india have shown that the infection is much more widespread than was previously su ... | 1976 | 1261753 |
| control of brucellosis in kuwait by vaccination of cattle, sheep and goats with brucella abortus strain 19 or brucella melitensis strain rev. 1. | in kuwait, approximately 12,000 dairy cows were vaccinated with a reduced dose of 3 x 10(9) brucella abortus strain 19 and approximately 350,000 sexually mature sheep and goats with a reduced dose of 10(7) b. melitensis strain rev. 1. using the criteria of prevaccinal and postvaccinal incidences of antibodies, abortions, and human cases of brucellosis, the programme was very successful. widespread vaccination of adult animals is the most effective method of controlling brucellosis among cattle, ... | 1992 | 1306918 |
| isolation of brucella melitensis from aborting ruminants in jordan. | thirty-four isolates (16.5 per cent) of brucella melitensis were cultured from 206 samples from aborted, still-born or weak full-term animals and vaginal swabs from aborted animals. twenty-six of 31 isolates from sheep were b. melitensis biovar 3. two isolates were biovar 1 and one of these was the vaccine strain rev 1. the three remaining isolates were from vaginal swabs and were not biotyped since b. melitensis biovar 3 had been isolated from the aborted fetuses. one of two isolates from cattl ... | 1992 | 1452817 |
| serological and bacteriological study of brucellosis in camels in central saudi arabia. | sera from 2,630 apparently normal adult camels (camelus dromedarius) raised in central saudi arabia (riyadh and al-kharj cities) were examined serologically by the rose bengal and standard united states of america brucella plate agglutination tests. the overall seroprevalence of brucellosis in the restricted populations of tested camels was 8%. the seroprevalence of brucellosis among camels raised in small numbers in the backyards of 24 houses in riyadh and those intensively raised on one large ... | 1992 | 1472730 |
| treatment of brucella melitensis infection in sheep and goats with oxytetracycline combined with streptomycin. | six treatment regimens using oxytetracycline (otc) combined with streptomycin (st) were evaluated for eliminating brucella melitensis from 480 naturally-infected sheep and goats. cessation of shedding brucella from udder secretions and absence of brucella in selected tissues at autopsy were considered criteria for successful treatment. four regimens were equally effective in eliminating brucella in the treated groups of sheep and goats regardless of the source of antibiotics used. these were reg ... | 1992 | 1472731 |
| unrecognised neurobrucellosis giving rise to brucella melitensis peritonitis via a ventriculoperitoneal shunt. | | 1992 | 1486897 |
| conjunctival vaccination of pregnant ewes and goats with brucella melitensis rev 1 vaccine: safety and serological responses. | when brucella melitensis strain rev 1 vaccine (rev 1) is administered by the standard method (1-2 x 10(9) viable bacteria injected subcutaneously), it may induce long-lasting serological responses and/or cause abortion in pregnant animals. the conjunctival route considerably reduces these drawbacks. in the present experiment a 1 x 10(8) cfu dose for both ewes and goats conjunctivally vaccinated at mid-pregnancy was tested for innocuousness (outcome of pregnancy, contamination of unvaccinated con ... | 1992 | 1610080 |
| probable breast-milk borne brucellosis in a young infant. | a young infant with acute brucellosis is reported. he presented with a septicaemia-like picture. diagnosis was based on a fourfold rise of brucella agglutination titres and a positive blood culture. he had been exclusively breast-fed when his mother developed brucellosis 4 weeks after delivery. it is strongly suspected that the transmission of brucella melitensis to this infant was through the maternal breast-milk. | 1990 | 1703749 |
| [serological survey of small ruminants in 4 districts of niger]. | in 1990, a serosurvey on 1,474 small ruminants was conducted in four districts of niger (maradi, zinder, diffa and dosso). the epidemiology and seroprevalence of eight diseases were studied: brucella melitensis and b. ovis brucellosis, chlamydiosis, coxiellosis, contagious caprine pleuropneumonia, type a pasteurellosis, peste des petits ruminants, rift valley fever, crimée-congo haemorragic fever. the main health problem for the development of small ruminants farming, seems to be both pasteurell ... | 1991 | 1843821 |
| the isolation of brucella melitensis biovar 3 from a testicular hygroma of a ram in a nomadic flock of sheep and goats in western sudan. | brucella melitensis biovar 3 was isolated from a testicular hygroma in a ram from a nomadic flock of sheep and goats serologically positive for brucellosis and with a history of occasional abortions. the affected testis was enlarged, extensively damaged and contained fluid. the isolation of this biovar identifies the need for further investigations into the prevalence of brucellosis and the particular biovars involved in nomadic animals. | 1990 | 2079561 |
| the interaction of brucella melitensis 16-m and caprine polymorphonuclear leukocytes. | in the present work, a study about the phagocytosis and intracellular killing of the polymorphonuclear leukocytes (pnmls) of goat in animals clinically healthy, vaccinated and inoculated experimentally with brucella melitensis has been done. during 6 weeks postvaccination and postinfection, the evolution in these animals has been studied. although animals were inoculated or vaccinated, there was influence in the phagocytosis and intracellular killing phases, even though a very low indices in thi ... | 1990 | 2208970 |
| dot-enzyme immunoassay for visual detection of brucella melitensis antibodies. | an indirect enzyme-linked immunosorbent assay, using antigen coupled to paper, has been adapted for the detection of brucella melitensis antibodies. optimum conditions were achieved by incubation of 1 ml of diluted serum with a single piece of paper coated with purified brucella antigens for a period of one hour, and by addition of a goat anti-human enzyme conjugate antibody for one hour again. under these conditions 80 human sera were examined and the results obtained were compared with wright ... | 1989 | 2490400 |
| isolation of brucella melitensis biovar 3 from a chamois (rupicapra rupicapra) in the southern french alps. | systemic brucellosis caused by brucella melitensis biovar 3 was identified in a chamois (rupicapra rupicapra) near the parc national des ecrins in the southern french alps (france). clinical signs included orchiepididymitis, polyarthritis, blindness and various neurological signs; necropsy findings included numerous calcified foci in testis, epididymis, kidney, subcutaneous connective tissue and brain. brucella sp. were identified in brain by indirect immunofluorescence and b. melitensis biovar ... | 1990 | 2304191 |
| analysis of immune response: comparison of immunoblots after isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis using cytoplasmic protein extract from brucella. | analysis of the immune response towards the facultative intracellular bacterium, brucella melitensis, was studied by immunoblotting after either isoelectric focusing (ief) or sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). a cytoplasmic extract (cpe) of brucella melitensis was used as antigen to analyse the response in 17 sera from naturally infected goats. cpe analysed by ief exhibited 25 proteins within the ph range of 4.35 to 6. immunoblotting revealed most of the staine ... | 1990 | 2227361 |
| the isolation and serology of brucella melitensis in a flock of goats in central rsa. | brucella melitensis biotype 1 was isolated in pure culture from the lungs, liver, spleen, kidney, stomach contents, abomasum and brain of an aborted caprine (boer goat) foetus in the district of cullinan near pretoria. the 18 does and 1 ram in the flock of boer goates were examined serologically by means of the complement fixation (cf) test, using brucella abortus antigen. six weeks later they were examined again, using b. abortus as well as b. melitensis biotype 1 antigens. no significant diffe ... | 1990 | 2216349 |
| in vitro determination of phagocytosis and intracellular killing of brucella melitensis by goat's polymorphonuclear phagocytes. | one hundred and forty samples of goats serum taken from animals suspected of having brucellosis, have been studied by trying to assess the phagocytosis in vitro of brucella melitensis rev-1 by polymorphonuclear neutrophil phagocytes opsonized with antibrucella serum. the serum agglutination test and the coombs antiglobulin test were carried out with the serum samples. the opsonization ingestion, intracellular killing and germ killing power of the serum were also assessed. the results demonstrate ... | 1989 | 2776406 |
| [sanitary and technologic evaluation of the rural processing of fresh goat cheese in chile]. | a sanitary and technological diagnosis of the goat cheese rural process was carried out. the purpose was to obtain more information for the planning of a program aimed to the improvement of this small agroindustry. samples of milk, curdle, dry abomasum, rennet, water and cheese of 10% of the small industries of two rural villages in two agricultural seasons, were taken. moreover, dilutions of the utensils and goat udders were prepared. the samples were subjected to microbiological analysis of me ... | 1988 | 3154301 |
| brucellosis in the upper nile region--melut district, sudan (report). | in the melut district, south sudan human and animal blood samples were investigated for brucella abortus and brucella melitensis. 9.2% of the investigated cattle and 16.2% of the green long-tailed monkies were brucella abortus-positive. school children revealed a 3.7% positivity rate, melut's hospital patients 5.2% and village children and adults 2.2%. however, only a small number of hospital patients was investigated. a small percentage of sheep and goats was investigated for brucella melitensi ... | 1988 | 3275090 |
| control of brucella melitensis brucellosis in developing countries. | | 1987 | 3300717 |
| control of brucella melitensis infection in sheep and goats--a review. | | 1987 | 3307078 |
| risk of infection by brucella melitensis for people living near infected goats. | taking advantage of a local outbreak of brucellosis in a herd of goats, we have assessed the risk of infection for people living nearby. by means of serological tests we have shown that such a risk is small provided that direct contact with contaminated tissues or secretions of goats is avoided. | 1987 | 3312421 |
| acute brucella ileitis. | human brucellosis is an enteric fever in which systemic symptoms are generally more prominent than gastrointestinal complaints. during an outbreak of infection caused by brucella melitensis, linked to ingestion of unpasteurized goat milk cheese, we treated a child with radiographic evidence of acute ileitis. alimentary tract involvement in brucellosis is reviewed. | 1988 | 3337066 |
| conjunctival vaccination of young goats with brucella melitensis strain rev 1. | three groups each of 15 four-month old female kids were vaccinated with brucella melitensis strain rev 1 either conjunctivally (1.1 x 10(7) or 1.1 x 10(9) cfu) or subcutaneously (1.1 x 10(9) cfu). one group of 15 kids remained unvaccinated as control. all kids were mated when 9.5 month old. three months later, they were challenged conjunctivally with b melitensis strain h38 at a dose of 5.1 x 10(6) cfu. blood samples collected before vaccination and throughout the experiment were subjected to ro ... | 1987 | 3451685 |
| the changing epidemiology of human brucellosis in texas, 1977-1986. | from 1977-1986, a total of 331 laboratory confirmed cases of human brucellosis were reported in texas. the annual number of cases ranged from 13 in 1977 to 84 in 1983. males accounted for 66% of the cases. cases ranged in age from one to 92 years, with 57% between the ages of 20 and 49 years. brucella melitensis infections accounted for 66% of the bacteriologically confirmed cases. infection with brucella canis was identified in four patients. annual incidence rates were higher in hispanics in e ... | 1989 | 2787107 |
| selection of a brucella vaccine strain of low residual virulence by chemical mutagenesis. | following incubation of the oral vaccine brucella suis strain 2 with diethyl sulphate (des), a mutant designated strain s105 was selected by screening surviving bacteria for reduced virulence for mice. strain 105 also showed low residual virulence for guinea-pigs and, unlike the parent strain, did not initiate abortion in pregnant sheep and goats after parenteral administration. nevertheless, it was as effective as the parent strain in stimulating protective immunity to brucella melitensis when ... | 1989 | 2795638 |
| serological titres following vaccination of sheep and goats with brucella melitensis rev i vaccine. | | 1973 | 4197921 |
| brucella melitensis infection in female kids born into a heavily infected environment. | | 1970 | 5503825 |
| effect of living rev. 1 vaccine in producing long-term immunity against brucella melitensis infection in sheep in iran. | | 1967 | 5625649 |
| experimental brucella melitensis infection in local goats-opsonoc- ytophagic test on blood. | | 1967 | 5626592 |
| [inborn natural immunity to brucella melitensis. ii. genetic factors of hereditary immunity to brucella melitensis in goats]. | | 1969 | 5802750 |
| serological response of cattle, sheep and goats in kenya vaccinated with killed brucella melitensis strain h38 adjuvant vaccine. | the antibody responses of cattle, sheep and goats to the brucella melitensis h38 adjuvant vaccine were monitored by serum (tube) agglutination, complement fixation and rose bengal plate tests. high and persisting antibody titres were induced by a single dose of this vaccine in all three species. it would be difficult to classify reactors by commonly used diagnostic tests in a control or an eradication programme using the h38 adjuvant vaccine. | 1983 | 6346660 |
| [immunization of ewes and she-goats against experimental brucella melitensis infection. a comparison of rev 1 and h 38 vaccines]. | | 1984 | 6489637 |
| comparison of four serological tests in the diagnosis of caprine brucellosis. | results from four serological tests for diagnosing brucellosis--serum tube agglutination (sat), agar gel immunodiffusion (agit), rose bengal plate (rbpt) and complement fixation (cft)--were compared using sera from goats from farms infected with brucella melitensis. ninety-two goats were negative and 29 positive to all four tests. the remaining 85 reacted to one or more tests. the rbpt was the most sensitive test and the agit the most specific, when compared with the cft. the results suggest tha ... | 1980 | 6774391 |
| [bovine brucellosis caused by brucella melitensis in france]. | each brucella species is known to have a definite host preference. in this respect, brucellosis in cattle is primarily due to b. abortus. as part of an investigation of the species and biovars responsible for bovine brucellosis in france, 312 strains have been examined since 1978 by the methods recommended by the subcommittee on the taxonomy of the genus brucella. of these, 264 (85%) were indeed classified as b. abortus members. however the 48 others (15%) had all the characteristics that define ... | 1989 | 2930137 |
| studies on brucella melitensis rev. 1 vaccine in goats. | | 1981 | 7342582 |
| comparative evaluation of microagglutination test and serum agglutination test as supplementary diagnostic methods for brucellosis. | the diagnosis of brucellosis in cattle and small ruminants requires the use of more than one serological test. the complement fixation test (cft), the rose bengal test (rbt), and the serum agglutination test (sat) are among the most useful tests for routine diagnosis. the microagglutination test (mat) was developed as a simpler and more efficient test than the sat. the relative efficacy of this test compared with that of the sat was evaluated by using brucella-free sheep and goats prior to and a ... | 1995 | 7559970 |
| urban outbreak of goat cheese brucellosis. | in 1983 an outbreak of human brucellosis caused by brucella melitensis occurred among residents of a predominantly hispanic neighborhood in houston, tx. the source of the infections was traced to unpasteurized goats' milk cheese imported from mexico. nineteen of the 31 patients who contracted the disease were members of 5 families, which included 11 children ranging in age from 2 to 17 years. infection was generally associated with protein complaints and a paucity of physical findings. both immu ... | 1986 | 3797296 |
| efficacy of different rose bengal and complement fixation antigens for the diagnosis of brucella melitensis infection in sheep and goats. | fifteen different rose bengal antigens showed large differences with respect to ph, cell concentration and agglutination with the international standard anti-brucella abortus serum, demonstrating the lack of international standardisation. their sensitivity and specificity, compared with that of the complement fixation test, were evaluated for the diagnosis of b melitensis infection in culture-positive sheep, brucella-free ewes, and sheep and goats belonging to field flocks under different epidem ... | 1994 | 8036772 |
| evaluation of serological tests for diagnosis of brucella melitensis infection of goats. | five serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (rbt), complement fixation test (cft), radial immunodiffusion (rid) with brucella and yersinia enterocolitica o:9 polysaccharides, counterimmunoelectrophoresis (ciep) with cytosol, and enzyme-linked immunosorbent assay (elisa) with polyclonal and protein g conjugates and smooth lipopolysaccharide (s-lps), native hapten polysaccharide (nh), or cytosol antigens. for optimal sensitivity, rbt had t ... | 1994 | 8051240 |
| identification of an immunoreactive brucella abortus htra stress response protein homolog. | an 11-kb fragment of brucella abortus genomic dna cloned into the bamhi site of puc9 expressed a 60-kda protein in escherichia coli dh5-alpha. antibodies reactive with this 60-kda protein were detected by western blot (immunoblot) analysis in sera from mice, cattle, and goats experimentally infected with b. abortus, in sera from mice experimentally infected with brucella melitensis, and in serum from a dog experimentally infected with brucella canis. similar results were seen with sera obtained ... | 1994 | 8112833 |
| urban outbreak of a brucella melitensis infection in an argentine family: clinical and diagnostic aspects. | an outbreak of brucella melitensis in a family was studied. from the fourteen family members who ate unpasteurized goat cheese nine became ill. patients included four females and five males of 8 to 75 years old. in seven of the patients the diagnosis was confirmed by positive blood culture for b. melitensis biovar 1. all the patients were analyzed by standard tube agglutination (sta) and standard tube agglutination with 2-mercaptoethanol (sta-2me) tests at the time of diagnosis. in six of the pa ... | 1994 | 8156050 |
| southwestern internal medicine conference: brucellosis: don't let it get your goat! | brucellosis is a highly pleomorphic zoonotic infection caused by one of the following four species of gram-negative facultative intracellular coccobacilli: brucella melitensis, b. abortus, b. suis, or b. canis. the disease is a worldwide public health problem and a significant cause of economic losses in domestic live-stock. although largely eradicated in most industrialized countries, in the united states there has been an upsurge of b. melitensis cases associated with the ingestion of unpasteu ... | 1994 | 8291510 |
| survey of brucellosis in goats and sheep in the yemen arab republic: comparison of tests for brucella melitensis infection in sheep. | sera from 538 yemeni goats and 690 yemeni sheep were screened for brucellosis by the rose bengal plate test (rbpt) and reactors confirmed by the complement fixation test (cft) and the serum agglutination test (sat). the prevalence among goats was 0.4% and among sheep 0.6%. the prevalence among 183 imported goats and sheep was 4.4%. the sensitivity and specificity of three serological tests available for the diagnosis of brucellosis -cft, rbpt and sat - were compared using ovine sera obtained thr ... | 1985 | 4024267 |
| the immune response of goats vaccinated with low and high doses of brucella melitensis rev 1. | the serological response, lymphocyte reactivity, and dermal hypersensitivity reactions of goats vaccinated with high and low doses of brucella melitensis rev 1 organisms to brucella antigens were studied. antibodies to soluble antigen a2 were detectable by immunoelectrophoresis (ie), appeared later than agglutinating antibodies but disappeared faster in most vaccinated animals. anti-a2 antibodies appeared earlier in goats which received the high dose. antibodies to polysaccharide b antigen were ... | 1982 | 7112914 |
| endocarditis caused by brucella melitensis. | to describe the management of an uncommon but serious infection in australia. | 1993 | 8479384 |
| brucellosis associated with unpasteurized milk products abroad. | | 1995 | 8519642 |
| single-step pcr for detection of brucella spp. from blood and milk of infected animals. | a versatile method for the extraction of brucella dna and pcr are presented as reliable tools for the detection of brucella spp. from body fluids of infected animals. two oligonucleotides homologous to regions of the gene encoding for an outer membrane protein (omp-2) were designed to detect the pathogen from milk and/or blood of infected goats, bovines, and human patients. the sensitivity of our test and its ability to detect the pathogen in samples from the field reveal a promising advance in ... | 1995 | 8586678 |
| bacterial persistence and immunity in goats vaccinated with a pure deletion mutant or the parental 16m strain of brucella melitensis. | to evaluate host responses, young goats were inoculated subcutaneously with a genetic deletion mutant (deltapure201) of brucella melitensis (n = 6), its virulent parental strain 16m (n = 6), or saline (n = 6). no clinical evidence of brucellosis was seen in any goat. serum antibody titers peaked at postinoculation day (pid) 14. bacteria in lymph nodes that drained sites of vaccination reached peak numbers of >10(6) cfu/g in both infected groups at pid 7 and progressively declined to pid 84. at n ... | 1996 | 8698463 |
| cloning of a brucella melitensis group 3 antigen gene encoding omp28, a protein recognized by the humoral immune response during human brucellosis. | brucella group 3 antigens (ags) are outer membrane proteins (omps) with a molecular mass ranging from 25 to 30 kda. the omps are of interest partially because of their potential use as vaccine and diagnostic reagents. we used human convalescent antibody (ab) to clone a gene that encoded a 28-kda protein from a lambdagt11 library of brucella melitensis 16m genomic dna. dna sequence analysis revealed a single open reading frame that would encode a protein of 26,552 da. the 28-kda protein had a pri ... | 1996 | 8698471 |
| comparison of two selective media for the isolation of brucella melitensis from naturally infected sheep and goats. | the efficacy of two selective media for the isolation of brucella melitensis from naturally infected sheep and goats was compared. two hundred and eighty sheep and 60 goats belonging to b melitensis-infected flocks were slaughtered and samples of milk (when available) and seven tissues were taken from each animal for bacteriological analyses. a modified thayer-martin's medium was more sensitive than farrell's medium; by the combined use of both media 142 infected sheep and 40 infected goats were ... | 1996 | 8733178 |
| childhood brucellosis in north-western greece: a retrospective analysis. | fifty-two cases of childhood brucellosis which occurred in north-western greece during the 15-year period 1979-1993, are reviewed. it is believed that they represent very closely the total incidence of the disease in the region which has a population of 100,000 children aged 0-14 years old. brucellosis-affected children were almost exclusively from goat- or shepherd families and of both sexes and all age groups. a broad spectrum of clinical manifestations ranging from malaise only to brain absce ... | 1996 | 8750800 |
| brucella melitensis biotype 1 outbreak in goats in northern kwazulu-natal. | | 1996 | 8786610 |
| brucella melitensis biotype 1 outbreak in goats in northern kwazulu-natal. | brucella melitensis biotype 1 was confirmed in indigenous, outbred goats in three northern districts of the kwazulu-natal province following the diagnosis of human malta fever in the same area. six foci of infection were found during an extensive serological survey involving 6266 goats carried out in most of the districts of the kwazulu-natal province. the prevalence in the positive herds varied between 17% and 100%. the diagnosis was confirmed by culturing milk samples from serologically positi ... | 1996 | 8856768 |
| antibody and delayed-type hypersensitivity responses to ochrobactrum anthropi cytosolic and outer membrane antigens in infections by smooth and rough brucella spp. | immunological cross-reactions between brucella spp. and ochrobactrum anthropi were investigated in animals and humans naturally infected by brucella spp. and in experimentally infected rams (brucella ovis infected), rabbits (brucella melitensis infected), and mice (b. melitensis and brucella abortus infected). in the animals tested, o. anthropi cytosolic proteins evoked a delayed-type hypersensitivity reaction of a frequency and intensity similar to that observed with b. melitensis brucellin. o. ... | 1997 | 9144364 |
| lymphocyte proliferative responses of goats vaccinated with brucella melitensis 16m or a delta pure201 strain. | the response to a brucella melitensis purek deletion mutant, delta pure201 (referred to as strain 201), was compared with the response to its parental strain, 16m, in juvenile goats. proliferative responses to gamma-irradiated bacteria were detected earlier in strain 201-infected goats. lymphocytes from strain 16m- or 201-infected goats proliferated in response to one-dimensional polyacrylamide gel electrophoresis-separated proteins of similar mass isolated from strain 16m or brucella abortus rb ... | 1997 | 9199478 |
| brucellosis: an overview. | brucellosis remains a major zoonosis worldwide. although many countries have eradicated brucella abortus from cattle, in some areas brucella melitensis has emerged as a cause of infection in this species as well as in sheep and goats. despite vaccination campaigns with the rev 1 strain, b. melitensis remains the principal cause of human brucellosis. brucella suis is also emerging as an agent of infection in cattle, thus extending its opportunities to infect humans. the recent isolation of distin ... | 1997 | 9204307 |
| a review of the use of b. melitensis rev 1 vaccine in adult sheep and goats. | the live brucella melitensis rev 1 strain is considered the best vaccine available for the prophylaxis of brucellosis in small ruminants. the classically recommended exclusive vaccination of young replacement animals has failed to control brucellosis in some developed countries and is frequently inapplicable in the developing world. accordingly, whole-flock vaccination is the only feasible alternative to control b. melitensis infection in small ruminants under the extensive management conditions ... | 1997 | 9234451 |
| [fever and dry cough in a construction worker from portugal]. | a 33-year-old portugese worker presented with a one-week history of nonproductive cough and fever. a presumptive diagnosis "viral infection of the respiratory tract" was made. however, because of persisting cough and fever further investigations were necessary, and finally brucella melitensis was isolated in blood cultures. three months before admission to the hospital the man was dressing the carcasses of a goat in portugal and consumpted fresh goats milk cheese. antibiotic therapy with rifampi ... | 1997 | 9340710 |
| the differentiation of brucella species by substrate specific tetrazolium reduction. | in the development of a tetrazolium reduction assay to replace substrate stimulated oxygen uptake for the identification of brucella species, nine tetrazolium salts were evaluated. only the more readily reduced compounds (mtt and int) detected increased metabolic activity with the more fastidious, slow growing strains and with b. suis strains on l-arginine and dl-ornithine. the assay was optimised with mtt. mtt reduction profiles offered with the medium on which the cells were grown. cells grown ... | 1997 | 9355260 |
| a brucella melitensis high-temperature-requirement a (htra) deletion mutant is attenuated in goats and protects against abortion. | it has been previously demonstrated that a brucella melitensis high-temperature-requirement a (htra) deletion mutant is more susceptible to oxidative killing in vitro than the parental strain and is attenuated in mice. to evaluate the contribution of the b melitensis htra protease to virulence in ruminants, the capacity of the b melitensis htra mutant rwp5 to produce abortion in goats was compared to that of the virulent parental strain 16m. experimental infection with strain 16m caused abortion ... | 1997 | 9429252 |
| human infection by brucella melitensis: an outbreak attributed to contact with infected goats. | although several outbreaks of brucella melitensis infection have been reported among laboratory workers or goat cheese consumers, outbreaks related to rural labour have been rarely studied. an outbreak of human brucellosis among farm workers of argentina was studied and revealed a close relationship with an epidemic of caprine abortions which occurred shortly before on the same farm. high rates of b. melitensis infection were found among goats. active brucellosis was diagnosed in 33 subjects (14 ... | 1997 | 9537757 |
| isolation of brucella melitensis from alpine ibex. | eleven alpine ibex (capra ibex) and 27 chamois (rupicapra rupicapra) from gran paradiso national park (italy) were examined in march 1996. a 7-yr-old ibex buck had thick-walled carpal joints and enlargement of the right testicle characterized by necrosis and fibrosis. microscopically, testicular lesions were characterized by large areas of necrosis, fibrosis with irregular aggregates of macrophages and lymphocytes, and scattered foci of suppuration. specimens of the carpal bursae and testicle we ... | 1998 | 9577795 |
| evaluation of north american antibody detection tests for diagnosis of brucellosis in goats. | the sensitivities and specificities of 17 antibody detection tests for brucellosis in goats were estimated. tests evaluated included the u.s. department of agriculture (usda) card test with 8% cell concentration (8%card), usda rapid automated presumptive test (rap), mexican rose bengal plate tests with 8 and 3% cell concentrations (8%rb and 3%rb), french rose bengal plate test with 4.5% cell concentration (4.5%rb), usda standard plate test (spt), usda buffered acidified plate agglutination test ... | 1998 | 9620406 |
| evaluation of a rough mutant of brucella melitensis in pregnant goats. | brucella melitensis strain vtrm1, a rough derivative of b melitensis strain 16m, is able to colonise the lymph nodes of goats, does not induce abortion in pregnant goats when used at doses leading to abortions with virulent strain 16m, and does not induce anti-o chain antibodies. however, strain vtrm1 as a single dose vaccine induces only partial protection against both infection and abortion following challenge. | 1998 | 9690613 |
| evaluation of lipopolysaccharides and polysaccharides of different epitopic structures in the indirect enzyme-linked immunosorbent assay for diagnosis of brucellosis in small ruminants and cattle. | brucella abortus and brucella melitensis have surface lipopolysaccharides and polysaccharides carrying b. melitensis-type (m) and b. abortus-type (a) epitopes as well as common (c) epitopes present in all smooth brucella biotypes. crude lipopolysaccharides, hydrolytic o polysaccharides, and native hapten polysaccharides of mc or ac specificity were evaluated in indirect enzyme-linked immunosorbent assays with polyclonal, monoclonal, or protein g conjugates by using sera from cattle, sheep, and g ... | 1998 | 9801329 |
| risk factors for brucellosis seropositivity of goat herds in the mexicali valley of baja california, mexico. | a case-control study was conducted in the mexicali valley to identify risk factors for goat-herd seropositivity for brucella melitensis. nineteen case herds (> or = 2 positive results with the 8% rose bengal plate test (rbt)) and 55 control herds (zero positive results in rbt), matched for herdsize and geographic location, were enrolled. conditional logistic regression was used to construct a multivariable model of the odds of seropositivity using variables assessed in a questionnaire administer ... | 1998 | 9879591 |
| use of mass vaccination with a reduced dose of rev 1 vaccine for brucella melitensis control in a population of small ruminants. | mass vaccination with reduced dose 1/50 rev 1 strain live vaccine (1-2 10(9) colony forming units), administered subcutaneously, over a four and a half year period reduced the prevalence of brucella melitensis in kuwait's small ruminant population from 5.8% in 1993 to 2.02% in 1997. serological test results using the rose bengal plate test, rivanol agglutination test and complement fixation showed no evidence of persistence of positive serology in animals nine or more months after vaccination. q ... | 1999 | 10445249 |
| brucellosis outbreak: analysis of risk factors and serologic screening. | israel is one of the mediterranean countries in which brucellosis is endemic. as recently there has been a brucellosis outbreak in a kibbutz, the aim of this study is to identify asymptomatic infected kibbutz members, and to delineate the manner of infection in this setting. therefore, all the asymptomatic kibbutz members were screened by the rose bengal test for brucellosis, while both patients and healthy members were asked to fill in a questionnaire in order to pinpoint the manner of infectio ... | 1999 | 10567679 |
| molecular characterization of brucella strains isolated from marine mammals. | recently, gram-negative bacteria isolated from a variety of marine mammals have been identified as brucella species by conventional phenotypic analysis. this study found the 16s rrna gene from one representative isolate was identical to the homologous sequences of brucella abortus, b. melitensis, b. canis, and b. suis. is711-based dna fingerprinting of 23 isolates from marine mammals showed all the isolates differed from the classical brucella species. in general, fingerprint patterns grouped by ... | 2000 | 10699036 |
| brucella melitensis infection discovered in cattle for first time, goats also infected. | | 2000 | 10707672 |
| [incidence, etiology and epidemiology of brucellosis in a rural area of the province of lleida]. | this a prospective study of the incidence, etiology and epidemiological profile of human brucellosis in the regions of pallars jussà y sobirà (lleida) for the 1995-1998 period. | 2000 | 10832390 |
| [brucellosis presenting as acute abdomen]. | usually symptoms of brucellosis are nonspecific and characterized by a wide range of complaints. although the disease in israel is almost exclusively food borne (caused by brucella melitensis in unpasteurized goat milk products) so the main route of infection is the gastrointestinal tract, but gastrointestinal complications are rare, and only sporadic cases of ileitis or colitis have been described. we present a 43-year-old woman with an acute abdomen, probably due to diverticulitis. it was diag ... | 1999 | 10914217 |
| isolation of chlamydia psittaci involved in abortion of goats in mexico: first report. | a group of 35 healthy adult goats ranging from two to six years old were bought from a chlamydiosis and brucellosis-free flock; they were vaccinated against brucella melitensis with rev1 vaccine at reduced doses, and one month later placed in isolation units. the animals were one month pregnant at the moment of purchase, and during the third month of pregnancy 10 out of the 35 dams aborted. necropsy of the aborted fetus and examination of the foetal membranes was performed where no macroscopic l ... | 1997 | 10932720 |
| assessment of a monoclonal antibody-based competitive enzyme linked immunosorbent assay (celisa) for diagnosis of brucellosis in infected and rev. 1 vaccinated sheep and goats. | in this study a celisa for the diagnosis of brucellosis due to b. melitensis in sheep and goats was evaluated and its capability of discriminating vaccinated from infected animals was assessed. information is provided indicating that the celisa has a diagnostic sensitivity (99.4%) and specificity (98.9%) in sheep and goats comparable to that of many standard indirect elisa methods. in addition, the test proved able to distinguish between vaccinated and infected animals with an accuracy of up to ... | 2000 | 11061628 |
| identification and characterization of in vivo attenuated mutants of brucella melitensis. | brucella melitensis 16m is a gram-negative alpha2-proteobacterium responsible for abortion in goats and for malta fever in humans. this facultative intracellular pathogen invades into and survives within both professional and non-professional phagocytes. signature-tagged mutagenesis (stm) was used to identify genes required for the in vivo pathogenesis of brucella. a library of transposon mutants was screened in a murine infection model. out of 672 mutants screened, 20 were not recovered after a ... | 2000 | 11069678 |
| [meningitis as the first and only manifestation of brucellosis]. | an 8-year-old girl with no previous symptoms was admitted to hospital with aseptic meningitis syndrome. the girls father is a shepherd and there was a familial antecedent of brucellosis, but the patient did not consume unpasteurized goat milk products nor did she take part in the care of the sheep stock. the clinical evolution did not follow the usual development of a viral or partially treated bacterial meningitis. brucella melitensis was isolated in the cerebrospinal fluid and blood. specific ... | 2000 | 11083975 |
| comparative analysis of brucella serotype a and m and yersinia enterocolitica o:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats. | hapten polysaccharides of brucella smooth m and a serotypes were prepared from brucella sp. and yersinia enterocolitica o:9 by previously described hydrolytic (o chain) or nonhydrolytic (native hapten [nh]) procedures. the purified polysaccharides differed only in the presence (o chain) or absence (nh) of lipopolysaccharide core sugars. the polysaccharides were compared by reverse radial immunodiffusion for the diagnosis of brucellosis in cattle (brucella abortus biotype 1 [a serotype] and bruce ... | 1993 | 8308104 |
| effect of age and duration of disease on the clinical manifestations of brucellosis. a study of 73 consecutive patients in israel. | we describe our experience with 73 patients diagnosed with brucellosis during the years 1979-91 at two jerusalem hospitals: hadassah mount scopus (37 patients from 1979-1984) and shaare zedek (36 patients from 1979-1991). the patients included 32 children less than 14 years old and 41 adults; 70 of the patients were non-jews. in all cases the pathogen was brucella melitensis. the high proportion of children and the equal sex distribution was quite different from the age and sex distribution of b ... | 1993 | 8454438 |
| bacterial infections: osteoarticular brucellosis. | osteoarticular brucellosis has been documented extensively from the middle east and spain in the last 5 years, but it has only been reported infrequently from the uk and usa. brucella melitensis from goat and sheep is the most frequently isolated organism. peripheral articular pain, particularly of the large joints, is the commonest osteoarticular manifestation, while effusions that seldom yield organisms on culture, also occur frequently. sacroiliitis which most frequently is unilateral, often ... | 1995 | 7728879 |
| acute cholecystitis caused by brucella melitensis: case report and review. | brucellosis is a common zoonosis in many parts of the world. human brucellosis is a multisystemic disease with a wide range of clinical symptoms. biliary involvement in the form of acute cholecystitis is a very rare manifestation of brucellosis. here we report the case of a 34-year-old male with acute cholecystitis caused by brucella melitensis and review seven other cases. we conclude that brucella may have a focal effect on the gallbladder, in the form of acute cholecystitis. | 2001 | 11243761 |
| [bacteremia caused by brucella sp. with negative conventional serology]. | | 2001 | 11256247 |
| re-examination of the role of the brucella melitensis htra stress response protease in virulence in pregnant goats. | based on previously reported studies describing the experimental infection of pregnant goats with b. melitensis strain rwp5, we proposed that the htra protease plays an important role in the virulence of b. melitensis in its natural ruminant host. subsequent studies, however, have shown that rwp5 is actually an htra cycl double mutant. in order to definitively evaluate the role of the b. melitensis htra in virulence, we constructed an authentic htra mutant and examined this strain in pregnant go ... | 2001 | 11423199 |
| use of recombinant bp26 protein in serological diagnosis of brucella melitensis infection in sheep. | previously a brucella protein named cp28, bp26, or omp28 has been identified as an immunodominant antigen in infected cattle, sheep, goats, and humans. in the present study we evaluated antibody responses of infected and b. melitensis rev.1-vaccinated sheep to the bp26 protein using purified recombinant bp26 protein produced in escherichia coli in an indirect enzyme-linked immunosorbent assay (i-elisa). the specificity of the i-elisa determined with sera from healthy sheep (n = 106) was 93%. the ... | 2001 | 11427425 |
| brucellosis control in saudi arabia: prospects and challenges. | brucellosis is a zoonotic disease of worldwide distribution. despite its control in many developed countries the disease remains endemic in saudi arabia where the national seroprevalence of the disease is 15%. in saudi arabia the disease is introduced through uncontrolled importation of animals that are poorly screened for the disease. every year the kingdom imports a few million heads of sheep and goats for sacrifice during hajj from africa, india, and autstralia. brucella melitensis remains th ... | 2001 | 11434523 |
| brucellosis among animals and human contacts in eastern sudan. | to study the relative frequency of brucellosis among domestic animals in kassala state, sudan, in the year 1999 and compare the results of previous studies since 1908. also to study the frequency rates of the disease in animal contacts in the area. | 2001 | 11479636 |
| [historical brief on the fever of malta]. | malta fever (brucellosis) is an infectious disease of cattle (chiefly goats) which can infect man. although the disease is not limited to malta, occurring also in other mediterranean countries as well as in asia and america, it was originally described in 1863 in malta where the responsible bacteria (brucella melitensis) was discovered in 1887 by d. bruce. ten years later wright & smith (1897) showed that the agglutination test originally established for typhoidic patients could be used for the ... | 1996 | 11624839 |
| risk factors for brucella seropositivity in goat herds in eastern and western uganda. | cross-sectional prevalences and risk factors for brucella seropositivity in goats in eastern and western uganda were investigated. serum was collected from 1518 goats randomly selected from 145 herds which had been identified using multistage sampling. the brucellosis card test (ct) and the brucella melitensis tube-agglutination test (tat) were used in parallel to detect antibodies against b. abortus and b. melitensis, respectively. interviewer-administered questionnaires were used to collect in ... | 2001 | 11679168 |
| field investigations of brucellosis in cattle and small ruminants in syria, 1990-1996. | the authors present the epidemiological status of brucellosis in cattle and small ruminants in syria from 1990 to 1996, based on laboratory findings at the brucellosis centre, damascus. initial investigations using the rose bengal plate test, the complement fixation test and a miniaturised variant of the slow agglutination test were conducted throughout the country in 1990 and 1991, revealing an overall herd seroprevalence rate of 3.14% in cattle herds and 2.94% in small ruminant flocks. althoug ... | 2001 | 11732419 |
| the genome sequence of the facultative intracellular pathogen brucella melitensis. | brucella melitensis is a facultative intracellular bacterial pathogen that causes abortion in goats and sheep and malta fever in humans. the genome of b. melitensis strain 16m was sequenced and found to contain 3,294,935 bp distributed over two circular chromosomes of 2,117,144 bp and 1,177,787 bp encoding 3,197 orfs. by using the bioinformatics suite ergo, 2,487 (78%) orfs were assigned functions. the origins of replication of the two chromosomes are similar to those of other alpha-proteobacter ... | 2002 | 11756688 |
| identification of the brucella melitensis vaccine strain rev.1 in animals and humans in israel by pcr analysis of the psti site polymorphism of its omp2 gene. | adverse effects of strain persistence and secretion in milk have been encountered with the brucella melitensis vaccine strain rev.1. field isolates obtained from vaccinated animals and from a human resembled the vaccine strain rev.1 by conventional bacteriological tests. the lack of a specific molecular marker that could specifically characterize the commercial vaccine strain prevented confirmation of the homology of the rev.1-like field isolates to the vaccine strain. the composition of the omp ... | 2002 | 11923376 |
| spinal brucellosis: case report in the united states. | case report. | 2002 | 11979184 |
| identification of a quorum-sensing signal molecule in the facultative intracellular pathogen brucella melitensis. | brucella melitensis is a gram-negative alpha2-proteobacterium responsible for abortion in goats and for malta fever in humans. this facultative intracellular pathogen invades and survives within both professional and nonprofessional phagocytes. a dichloromethane extract of spent culture supernatant from b. melitensis induces bioluminescence in an escherichia coli acyl-homoserine lactone (acyl-hsl) biosensor strain based upon the activity of the lasr protein of pseudomonas aeruginosa. hplc fracti ... | 2002 | 12010991 |
| identification of brucella melitensis vaccine strain rev.1 by pcr-rflp based on a mutation in the rpsl gene. | the live attenuated strain b. melitensis rev.1 is considered the best vaccine available for the prophylaxis of brucellosis in sheep and goats. the rev.1 vaccine was obtained in the 1950s by a two-step selection involving firstly streptomycin resistance and dependence and secondly reversion of dependence but keeping streptomycin resistance. chromosomally acquired streptomycin resistance is frequently due to mutations in the gene encoding the ribosomal protein s12, rpsl. nucleotide sequencing reve ... | 2002 | 12057611 |
| pathogenicity and protective activity in pregnant goats of a brucella melitensis deltaomp25 deletion mutant. | the brucella melitensis mutant bm 25, which lacks the major 25 kda outer membrane protein omp25, has previously been found to be attenuated in the murine brucellosis model. in the present study, the capacity of the deltaomp25 mutant to colonise and cause abortions in the caprine host was evaluated. the vaccine potential of bm 25 was also investigated in goats. inoculation of nine pregnant goats in late gestation with the b. melitensis mutant resulted in 0/9 abortions, while the virulent parental ... | 2002 | 12076120 |
| bacteria-modified amperometric immunosensor for a brucella melitensis antibody assay. | a novel amperometric immunosensor setup is described which uses horseradish peroxidase (hrp) as a label in conjunction with a current-based brucella sensor. the bacteria modified immunosensor was constructed by using a biocomposite formed by dispersing graphite powder into a mixture of brucella melitensis and silicate polymer gel. the enzyme-labeled antibody can readily diffuse toward the encapsulated antigen (brucella melitensis), which retains its binding properties, and the association reacti ... | 2002 | 12083544 |
| global analysis of the brucella melitensis proteome: identification of proteins expressed in laboratory-grown culture. | brucella melitensis is a facultative intracellular bacterial pathogen that causes brucellosis, a zoonotic disease primarily infecting sheep and goats, characterized by undulant fever, arthritic pain and other neurological disorders in humans. a comprehensive proteomic study of strain 16m was conducted to identify and characterize the proteins expressed in laboratory-grown culture. using overlapping narrow range immobilized ph gradient strips for two-dimensional gel electrophoresis, 883 protein s ... | 2002 | 12203900 |