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associations between microbial species in dental root canal infections.the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ...19921494447
microflora associated with experimental root surface caries in humans.this study describes the microflora from actively progressing root surface caries lesions, in which mineral loss had been determined by quantitative microradiography. the caries lesions were produced experimentally in root surface specimens from human molars inserted in lower partial dentures carried for 3 months by six elderly individuals. a total of 780 bacterial isolates were identified from 13 plaque samples, collected with a punch technique, and six dentin samples. the composition of the mi ...19902341169
prevalence of viridans streptococci exhibiting lactose-inhibitable coaggregation with oral actinomycetes.fresh oral isolates from human dental plaque were selected on the basis of their spherical morphology. in a double-blind experiment, their species identity and ability to coaggregate with human oral actinomyces viscosus and actinomyces naeslundii were determined. of the 110 isolates characterized, 30 were identified as either streptococcus mutans, streptococcus anginosus-constellatus, or veillonella parvula; none of these coaggregated with the actinomycetes. the remaining 80 isolates were identi ...19836409806
establishment of human indigenous bacteria in germ-free mice.gibbons, r. j. (forsyth dental center and harvard school of dental medicine, boston, mass.), s. s. socransky, and b. kapsimalis. establishment of human indigenous bacteria in germ-free mice. j. bacteriol. 88:1316-1323. 1964.-thirteen strains of bacteria indigenous to the gingival crevice area of man were tested for their ability to establish as monocontaminants in germ-free mice. three facultative organisms, streptococcus mitis, staphylococcus albus, and a "diphtheroid," established, as well as ...196414234787
pattern of cytokine responses to gram-positive and gram-negative commensal bacteria is profoundly changed when monocytes differentiate into dendritic cells.the normal gastrointestinal bacterial flora is crucial for the maturation of acquired immunity via effects on antigen-presenting cells (apcs). here we investigated how two types of apcs, monocytes and dendritic cells (dcs), react to different bacterial strains typical of the commensal intestinal microflora. purified human monocytes and monocyte-derived dcs were stimulated with uv-inactivated gram-positive (lactobacillus plantarum and bifidobacterium adolescentis) and gram-negative (escherichia c ...200415102775
on the occurrence of the lactate fermenting anaerobe, micrococcus lactilyticus, in human saliva. 195015428563
activation of toll-like receptors 2 and 4 by gram-negative periodontal bacteria.periodontitis is a chronic infectious disease associated with a gram-negative subgingival microflora. bacterial components stimulate, among other receptors, toll-like receptor (tlr) 2 and/or tlr4. accumulating evidence indicates that both qualitatively and quantitatively distinct immune responses result from the triggering of tlr2 as compared to tlr4 triggering. the aim was to study the interaction of porphyromonas gingivalis, actinobacillus actinomycetemcomitans, tannerella forsythensis, prevot ...200717488439
targeted profiling of oral bacteria in human saliva and in vitro biofilms with quantitative real-time pcr.an in vitro plaque model based on the use of human salivary bacteria and tooth-like surfaces was previously developed for studying the formation of oral biofilm and its use for pre-clinical testing of candidate antimicrobial or antiplaque agents. in this study, a quantitative taqman pcr assay (qpcr) was developed to compare the bacterial compositions of in vitro biofilms to parent saliva samples, and to determine the relative contributions of different species in the formation of the oral biofil ...200717653931
predominant bacteria recovered from a periodontitis site in a hamster model raised by silk-ligature with porphyromonas gingivalis infection.we isolated oral bacteria that coexisted with porphyromonas gingivalis in a hamster periodontitis model. as predominant bacteria in the periodontitis site, collinsella-reltaed strains, eubacterium-reltaed strains, streptococcus suis-related strains, and veillonella parvula-reltaed strains were detected. in addition, actinomyces, bacteroides, and p. gingivalis were also isolated predominantly. the results suggest that the bacterial composition of the periodontitis site in hamsters is complex, as ...200818460794
identification of oral species of the genus veillonella by polymerase chain reaction.members of the genus veillonella cannot be reliably distinguished by their biochemical characteristics and phenotypic features. moreover, dna-dna hybridization and sequence analyses of the 16s ribosomal rna gene including random fragment length polymorphism analysis, are complex and time-consuming procedures that are not well-suited to identifying oral species of veillonella: veillonella atypica, veillonella denticariosi, veillonella dispar, veillonella parvula, and veillonella rogosae.200919572893
effects of sonic energy on monospecific biofilms of cariogenic microorganisms.to evaluate the in vitro differential effect of sonic energy, delivered by sonicare advance, on cariogenic and non-cariogenic bacteria biofilms.200616555648
was isolation of veillonella from spinal osteomyelitis possible due to poor tissue perfusion?from a needle biopsy of the body of the 12th thoracic vertebra in a 74-year-old man with spinal osteomyelitis a veillonella parvula was isolated. the significance of this bacterium as lactic acid indicator has been considered and discussed. since this strictly anaerobic bacterium mainly uses lactic acid for energy generation, lactic acid must continuously and sufficiently have been produced in or near to the vertebrate body to permit outgrowth of this bacterium. since neither microbial infection ...200415325011
endocarditis due to veillonella alcalescens.a 60-year-old man with a history of a benign heart murmur for 25 years had evaluation of six months of unexplained fever and heart disease. the fever responded to nonspecific antimicrobial therapy, but progressive aortic insufficiency necessitated aortic valve replacement. although numerous preoperative blood cultures were negative, veillonella alcalescens was isolated in pure culture from the excised valve. the patient remained well after two weeks of intravenous therapy with cephalothin and th ...19846484702
production of volatile fatty acids as a result of bacterial interactions in the cecum of gnotobiotic rats and chickens fed a lactose-containing diet.volatile fatty acid (vfa) productions from lactose and lactate by a clostridium butyricum and a veillonella alcalescens strain, alone or in combination with a lactobacillus acidophilus strain, were determined both in vitro in culture media and in vivo in the ceca of gnotobiotic animals. gnotobiotic rats, which possess intestinal lactase, and chickens, which are devoid of it, were used. both animal species were fed a diet containing 4% lactose. the in vitro results showed that the c. butyricum st ...19883148988
metabolism of gentiopicroside (gentiopicrin) by human intestinal bacteria.as a part of our studies on the metabolism of crude drug components by intestinal bacteria, gentiopicroside (a secoiridoid glucoside isolated from gentiana lutea), was anaerobically incubated with various defined strains of human intestinal bacteria. many species had ability to transform it to a series of metabolites. among them, veillonella parvula ss parvula produced five metabolites, which were identified as erythrocentaurin, gentiopicral, 5-hydroxymethylisochroman-1-one,5-hydroxymethylisochr ...19892605687
a comparison of human and animal mouth flora.human bite injuries are often believed to be more serious than animal bite injuries. one reason for this distinction might be a species difference in the microbial mouth flora. in order to compare this possibility, we cultured the mouths of humans, dogs, and cats. human oral flora contained the smallest number of bacteria followed by dog and cat oral flora, respectively. veillonella parvula was cultured from all humans and was also found in dog and cat mouths, but less frequently. v parvula is n ...19911744782
blastogenic response of human lymphocytes to oral bacterial antigens: comparison of individuals with periodontal disease to normal and edentulous subjects.cell-mediated immunity in humans to antigens derived from oral plaque bacteria was investigated by using the lymphocyte blastogenesis assay. subjects with varying severities of periodontal disease including normal, gingivitis, periodontitis, and edentulous were compared. mononuclear leukocytes were separated from peripheral blood and cultured with antigens prepared by sonication of actinomyces viscosus (av), actinomyces naeslundii (an), veillonella alcalescens (va), leptotrichia buccalis (lb), b ...197610259
genetic transformation of veillonella parvula.veillonellae are one of the most prevalent and predominant microorganisms in both the supra- and subgingival plaques of the human oral cavity. veillonellae's mutualistic relationships with the early, middle, and late colonizers of the oral cavity make them an important component of oral biofilm ecology. unlike other ubiquitous early colonizers in the oral cavity, surprisingly little is known about veillonella biology due to our lack of ability to genetically transform this group of bacteria. the ...201121707732
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