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transduction by bacteriophage t1.amber mutants of the virulent coliphage t1 are able to transduce a wide variety of genetic characteristics from permissive to nonpermissive k strains of escherichia coli.19704920089
requirement for protein synthesis for survival of unmodified bacteriophage t1 in a restricting host.at high multiplication of infection, a substantial fraction of restricting cells (p1 lysogens) could be productively infected by unmodified coliphage t1 (t1.0) provided that protein synthesis was uninhibited during the first 5 min of infection. successful infection under restricting conditions was accompanied by more genetic recombination than was seen under nonrestricting host, the recombination frequency declined for markers on t1.0 genomes; no effect was seen on recombination between markers ...19817014928
escherichia coli bacteriophage t1 dna methyltransferase appears to interact with escherichia coli enolase.infection of escherichia coli cells with bacteriophage t1 induces synthesis of a bacteriophage-specific dna methyltransferase (m.ecot1, ec no: 2.1.1.72) with a specificity for adenine residues in the sequence 5'-gatc-3'. purification of m.ecot1 allowed the determination of the coding sequence of the gene (schneider-scherzer et al., 1990). the peptide of the entire coding sequence was over-expressed as a histidine-hexapeptide tagged protein in e. coli. affinity purification using a ni2+ chelating ...19989628368
the structure of replicating bacteriophage t1 dna: comparison between wild type and dna-arrest mutant infections.analysis of the structure of replicating phage t1 dna has identified three major forms of t1+ intracellular dna; (i) concatemeric molecules with single-stranded interruptions, (ii) monomer-length dna with single-strand interruptions, and (iii) monomer-length molecules with completely intact single strands. the interruptions in concatemeric dna are spaced at intervals, with a mean distance equivalent to one monomer length but with a broad distribution and many are in the form of gaps. type (iii) ...19846328749
characterization of transduction by bacteriophage t1: time of production and density of transducing particles.the transducing activity of two different kinds of premature lysates of t1-infected cells have been compared to normal lysates. the results show that t1-transducing particles are made early in the maturation period. the average density of t1-transducing particles is slightly greater than the density of plaque-forming t1.19751100864
transduction of bacteriophage lambda by bacteriophage t1.when bacteriophage t1 was grown on bacteriophage lambda-lysogenic cells, phenotypically mixed particles were formed which had the serum sensitivity, host range, and density of t1 but which gave rise to lambda phage. t1 packaged lambda genomes more efficiently both when the length of the prophage was less than that of wild-type lambda and when the host cell was polylysogenic. expression of the red genes of lambda or the rece system of escherichia coli during t1 growth enhanced pickup of lambda by ...1979381686
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