| enteric adenovirus type 40:e1b transcription map and identification of novel e1a-e1b cotranscripts in lytically infected cells. | adenovirus 40 (ad40) is defective for growth in tissue culture but is complemented when the ad2/5 or ad12 e1b 55k protein is supplied in trans. ad40 e1b mrna has not been detected in e1-transformed cells, or at early times in lytically infected cells. in cells constitutively expressing the e1b region of ad2, ad40 e1b mrnas are detected at late times in infection, after the onset of dna replication. we have determined the ad40 e1b transcription map from rna produced at late times in infected kb16 ... | 1991 | 1825250 |
| fiber sequence heterogeneity in subgroup f adenoviruses. | the fiber gene of adenovirus type 41 was sequenced and compared to the fiber gene sequence of adenovirus type 40 (a. h. kidd and m. j. erasmus, 1989, virology 172, 134-144), the other known member of subgroup f. the open reading frame, from map units 87 through 92 with transcription from the r-strand, comprised 1686 bases and was 45 bases longer than its counterpart on the ad40 genome. the 45-base difference appears to have resulted from a block deletion on the ad40 sequence. apart from this one ... | 1990 | 2219717 |
| evaluation of a commercial monoclonal antibody-based enzyme immunoassay for detection of adenovirus types 40 and 41 in stool specimens. | a commercial monoclonal antibody-based enzyme immunoassay (adenoscreen; mercia diagnostics ltd., guildford, united kingdom) for the detection of adenovirus types 40 and 41 in stool specimens was evaluated. two assay modes were tested. in the first, 177 stool samples were screened for the presence of adenovirus type 40 or 41 (assay mode 1). virus was detected in 79 of 82 specimens positive for adenovirus type 40 or 41 by a polyclonal antibody-based immune electron microscope test, giving a sensit ... | 1989 | 2546968 |
| studies on fastidious adenoviruses in ontario: a distinct strain associated with gastroenteritis. | from 100 cases of gastroenteritis among children caused by adenovirus infection in ontario, 33 virus isolates were divided into three categories according to their biological behavior in tissue cultures. so far, the results of neutralization tests, structural protein analysis, and dna restriction patterns showed that the virus of category 1 was similar to adenovirus type 40. however, the adenovirus of category 2 was a distinct adenovirus which shared some similarities with adenovirus type 5. vir ... | 1986 | 3009537 |
| genome variants of human adenovirus 40 (subgroup f). | twenty-six strains of faecal adenovirus from three continents (two from europe, 16 from south africa, and eight from canada) were typed as ad40 by restriction enzyme analysis of the dna using sma i. the dna of the strains was further compared by digestion with six other enzymes, namely bam hi, eco ri, bgl i, xho i, sst i, and hind iii. at least two different restriction profiles were found to exist for each enzyme. nineteen of the 26 strains studied had the same combination of profiles for all t ... | 1984 | 6094722 |
| em study of fastidious adenovirus type 40 replication in vitro. | primary human embryo kidney cells and chang conjunctival cells were inoculated with fastidious enteric adenovirus type 40. mature virions were seen in the nuclei of infected chang cells, but not in human embryo kidney cells, indicating that at least a proportion of chang cells can support the entire replicative cycle. | 1984 | 6696637 |
| identification of adenoviruses in faeces from patients with diarrhoea at the hospitals for sick children, london, 1989-1992. | faecal samples from 137 patients that had been shown to contain adenoviruses by electron microscopy were identified in a series of enzyme immunoassays (eia) using a single monoclonal antibody (mab) to adenovirus 40 and four different mabs to adenovirus 41. adenoviruses were partially characterised by restriction enzyme analysis (rea) of dna extracts using smai. samples were also run in a commercial eia (adenovirus ideia; dako, ltd.) which detects group antigen. the majority (84%) of adenoviruses ... | 1994 | 8083654 |
| the dna sequence of adenovirus type 40. | the 34,214 bp dna sequence of adenovirus type 40 strain dugan was determined directly from random fragments of virion dna cloned into a bacteriophage m13 cloning vector. the gene layout is similar to that of other human adenoviruses, and in addition contains two potential protein-coding regions that are conserved, but have not been recognized previously, in other adenovirus genomes. one is oriented rightward, contained within the intron in the protein-coding region for the l4 33k gene, and would ... | 1993 | 8263936 |
| adenovirus type 40 and 41 growth in vitro: host range diversity reflected by differences in patterns of dna replication. | subgroup f adenoviruses adapt poorly to cell culture, but the reasons for their fastidious nature are as yet ill defined. in an attempt to gain an overview of the differences in replication between adenovirus type 2 (ad2) and representative strains of ad40 and ad41, cell lines which show different degrees of permissiveness to ad40 and ad41 were infected and examined with respect to three key functions in the ad2 life cycle: host protein shutoff, dna synthesis, and late antigen synthesis. the com ... | 1994 | 8289359 |
| inactivation of feline calicivirus and adenovirus type 40 by uv radiation. | little information regarding the effectiveness of uv radiation on the inactivation of caliciviruses and enteric adenoviruses is available. analysis of human calicivirus resistance to disinfectants is hampered by the lack of animal or cell culture methods that can determine the viruses' infectivity. the inactivation kinetics of enteric adenovirus type 40 (ad40), coliphage ms-2, and feline calicivirus (fcv), closely related to the human caliciviruses based on nucleic acid organization and capsid a ... | 2003 | 12514044 |
| inactivation of enteric adenovirus and feline calicivirus by ozone. | little information is available regarding the effectiveness of ozone on the inactivation of caliciviruses and enteric adenoviruses. inactivation experiments were conducted with feline calicivirus (fcv), closely related to the human caliciviruses based on nucleic acid organization and capsid architecture, and adenovirus type 40 (ad40). experiments were carried out in buffered disinfectant demand free water at ph 7 and 5 degrees c. ct values; concentration of ozone multiplied by contact time with ... | 2005 | 16061270 |
| rapid detection of norovirus from fecal specimens by real-time reverse transcription-loop-mediated isothermal amplification assay. | in this study, we developed a one-step, single-tube genogroup-specific reverse transcription-loop-mediated isothermal amplification (rt-lamp) assay for the detection of norovirus (nov) genomes targeting from the c terminus of the rna-dependent rna polymerase gene to the capsid n-terminal/shell domain region. this is the first report on the development of an rt-lamp assay for the detection of nov genomes. because of the diversity of nov genotypes, we used 9 and 13 specially designed primers conta ... | 2006 | 16597865 |