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combinations of two amino acids (ala40 and phe75 or ser40 and tyr75) in the coat protein of apple chlorotic leaf spot virus are crucial for infectivity.amino acid sequences of apple chlorotic leaf spot virus (aclsv) coat protein (cp) were compared between 12 isolates from apple, plum and cherry, and 109 cdna clones that were amplified directly from infected apple tissues. phylogenetic analysis based on the amino acid sequences of cp showed that the isolates and cdna clones were separated into two major clusters in which the combinations of the five amino acids at positions 40, 59, 75, 130 and 184 (ala(40)-val(59)-phe(75)-ser(130)-met(184) or se ...200717698674
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