| potential contribution of mild pepsin treatment at ph4 to the viral safety of human immunoglobulin products. | different manufacturers use several different processes for the production of intravenous immunoglobulin. several manufacturers include a production step where the immunoglobulin is treated with low levels of pepsin at ph 4. a series of experiments were undertaken to assess whether or not ph 4/pepsin treatment could inactivate a range of test viruses. acid-labile viruses such as vaccinia, herpes simplex, mumps and semliki forest virus were found to be susceptible to ph 4/pepsin treatment whereas ... | 1988 | 3142151 |
| [urogenital and anal papillomavirus infections]. | recently virologists and clinicians have focused attention on infections with human papillomaviruses (hpv). this is due to the ubiquity, the increasing frequency and the possible association of these viruses with the development of squamous cell carcinomas of the skin and of the mucous membranes of the respiratory, gastrointestinal, genitourinary and anorectal tracts. hpv represent a very heterogeneous group of dna tumor viruses. by means of molecular-biological techniques, more than 40 hpv type ... | 1986 | 3026998 |
| prevention of rhinovirus and poliovirus uncoating by win 51711, a new antiviral drug. | win 51711, a potent new antipicornavirus drug, has been shown to inhibit an early event in the replication cycle of human poliovirus type 2 and human rhinovirus type 2. win 51711 was not virucidal and had no measurable effect on the adsorption of [3h]uridine-labeled virions to cells. when virion penetration of the plasma membrane was determined through loss of sensitivity to neutralizing antisera, win 51711 had no effect on poliovirus penetration, but inhibited rhinovirus penetration by 40%. in ... | 1986 | 3019232 |
| papillomavirus dna in warts of immunosuppressed renal allograft recipients. | renal allograft recipients were shown to have an increased incidence of warts and skin cancers. we examined 148 patients for evidence of wart virus infections and tested for papillomavirus types, which are known to be associated with human malignancies. of the 148, 36 (24.3%) patients were afflicted with warts at the end of our study period, in contrast to 5 of 148 (3.3%) before transplantation. dna from 16 different biopsies was extracted by phenol treatment for further virological studies. dna ... | 1986 | 3015050 |
| identification of the human papilloma virus-1a e4 gene products. | antibodies prepared against a human papilloma virus-1 (hpv-1) e4/beta-galactosidase fusion protein identified several polypeptides in hpv-1, but not hpv-2 or 4, induced papillomas. the major e4 protein, that represented up to 30% of total cellular protein, was a 16/17-k doublet which was purified by column chromatography and analysed for amino acid content. a peptide derived by chymotryptic digestion was purified by h.p.l.c. and subjected to amino acid sequencing. the unique sequence obtained, g ... | 1986 | 3011404 |
| detection of ebv and hpv dna sequences in oral "hairy" leukoplakia by in situ hybridization. | oral "hairy" leukoplakia (ohl) is a white lesion of the oral mucosa, usually located on the lateral tongue among human immunodeficiency virus (hiv) positive acquired immunodeficiency syndrome (aids)-risk patients. the lesion has been reported to be associated with epstein-barr virus (ebv) and human papillomavirus (hpv). twenty surgical biopsy specimens were evaluated for the presence of hpv genus-specific antigen, hpv 2/4, 6/11, and 16/18 dna and ebv dna by in situ hybridization employing formal ... | 1988 | 2849634 |
| epidemiology of butchers' warts. | studies were carried out in two slaughter-houses in different cities differing in the degree of work automation and, for comparison, in workers of nearby factories of the same two cities. there was a high incidence of warts (49.2%) in a slaughterhouse where the workers had direct contact with animals and meat, while a significantly lower incidence (9%) was observed in a modern slaughterhouse where the work was almost completely automated. the types of human papillomaviruses (hpvs) were similar i ... | 1988 | 2841908 |
| oral squamous papillomas: detection of hpv dna by in situ hybridization. | oral squamous papillomas were segregated from other papillary lesions on the basis of histopathologic features. twenty representative papillomas were evaluated for the presence of papillomavirus genus-specific antigen with the use of an immunoperoxidase technique. these same tumors were analyzed for human papillomavirus (hpv) types 2, 4, 6, and 11 with biotinylated full-length double-stranded dna probes by in situ hybridization. only one case exhibited papillomavirus antigen reactivity. alternat ... | 1988 | 2836773 |
| humoral and cellular immunity to papillomavirus in patients with cervical dysplasia. | the cell-mediated and humoral immune responses to human papillomavirus (hpv) were tested in groups of patients with various degrees of cervical intraepithelial neoplasia (cin) using a lymphocyte proliferation assay (lpa) as a measure of circulating sensitised t-cells and an enzyme-linked immunosorbent assay (elisa) for antibodies. twenty-three of 92 patients (25%) gave stimulation indices (s.i.) greater than two to at least one of the several antigen preparations tested in the lpa. of 282 patien ... | 1988 | 2828535 |
| novel variants of human papillomavirus type 2 in warts from immunocompromised individuals. | twenty cutaneous warts from i8 renal transplant recipients (rtr) and a single wart sample from a patient suffering from hodgkin's disease were screened for the presence of human papillomavirus (hpv) sequences by southern blot analysis. thirteen samples were shown to contain hpv dna sequences, one was identified as hpv-3, two as hpv-4, six as hpv-2 and four were unidentifiable by restriction digest analysis. hpv-3 was identified in the wart sample from the patient suffering from hodgkin's disease ... | 1989 | 2557072 |
| human papillomavirus type 29 (hpv-29), an hpv type cross-hybridizing with hpv-2 and with hpv-3-related types. | the cloning and partial characterization of human papillomavirus (hpv) type 29 is presented. by hybridization analyses, this virus appears to be related to hpv types associated with common warts and hpv types associated with flat warts. | 1989 | 2552165 |
| presence and type specificity of papillomavirus antibodies demonstrable by immunoelectron microscopy tests in samples from patients with warts. | sera from 75 patients with warts were tested for antibodies against human papillomaviruses (hpvs) in an immunoelectron microscopy test. a group of 40 patients' sera were tested against a pooled suspension of the hpvs extracted from their warts and 90% of the sera were found to be antibody-positive. prior to serological investigation viruses from a second group of 35 patients were typed by dot blot hybridization with hpv-1 and hpv-2 dna. according to these results the patients were divided into t ... | 1990 | 2155296 |
| identification of candidate sequences that determine virulence in coxsackievirus b4. | we have previously shown that a major determinant of virulence for coxsackievirus b4 mapped to the 5' end of the viral genome. comparison of the corresponding cdna sequences of a virulent and a non-virulent virus has allowed the identification of candidate determinants of virulence in the 5' untranslated region and the capsid proteins vp1, vp2 and vp4. thirteen nucleotide substitutions were observed in a region spanning 3298 nucleotides. four mutations were detected in the non-coding region. of ... | 1992 | 1320798 |
| [effect of sodium salicylate on the "in vitro" development of rna viruses (author's transl)]. | sodium salicylate 10(-3) m is able to limit the number of plaques induced by rna viruses on monkey kidney cell cultures (cercopithecus aethiops and macaccus rhesus). however, the inhibition rate changes with the virus type or even the strain used. poliovirus 2, coxsackie b3, echovirus and reovirus 1 were the most susceptible to the inhibitory effect of the drug. latent infection caused by sv5 decreases the antiviral activity of salicylate. the stages of the poliovirus type 2 growth affected by s ... | 1975 | 171988 |
| the effects of concanavalin a on the early events of infection by rhinovirus type 2 and poliovirus type 2. | the effect of concanavalin a (con a) on the course of early infection of hela cells with purified radioactive human rhinovirus type 2 (hrv-2) or poliovirus type 2 (p-2) has been examined. several early steps in infection were inhibited before the uncoating of parental virus. con a, at 100 mug/ml, reduces attachment of virus when added to cells before infection. con a also detectably slows the normal progression of adsorbed virus to tightly bound forms characterized, in the case of hrv-2, by resi ... | 1975 | 170375 |
| anti-picornavirus activity of synthetic flavon-3-yl esters. | the in vitro antiviral activity against picornaviruses (rhinovirus serotype 1b and 14, and poliovirus type 2) of new synthetic 3-hydroxyflavones, 3-acetoxyflavones, and substituted cinnamic and benzoic acid flavon-3-yl esters was evaluated. the maximum non-toxic concentration of compounds was determined in a human cell line (hela) suitable for the replication of the three viruses. their antiviral potency was measured by a plaque reduction assay. generally, rhinoviruses exhibited a higher sensiti ... | 1998 | 9865389 |
| common association of hpv 2 with anogenital warts in prepubertal children. | anogenital (ag) warts in 31 prepubertal children were hpv typed by nonisotopic in situ hybridization (nish) using digoxigenin-labeled probes for human papilloma virus (hpv) types 1-5, 6, 11, 16, 18, 31, and 33. mode of transmission was determined from historical, clinical, and laboratory data independent of hpv typing. hpv 2 was detected most commonly (13/31 warts) followed by hpv 6 (7/31), hpv 11 (5/31), and hpv 16 (1/31). although not reaching statistical significance, our results suggested th ... | 1997 | 9336800 |
| sch 38057: a picornavirus capsid-binding molecule with antiviral activity after the initial stage of viral uncoating. | the activity of a new water-soluble molecule, sch 38057, against picornaviruses is described. sch 38057 inhibited plaque formation of selected entero- and rhinoviruses in a range of 10.2 to 29.1 microm (50% endpoint) and had a therapeutic index of 10 against poliovirus type 2 (polio 2) in hela cells. when administered orally or subcutaneously, sch 38057 protected mice infected with either coxsackievirus b3 (cvb3) or echovirus-9 from mortality. the molecule provided a low level of protection agai ... | 1993 | 8391247 |
| papillomavirus infection and its pathologic correlate in sexually active adolescents. | we investigated the prevalence of human papillomavirus (hpv) and its pathologic correlation in an adolescent clinic population (13-20 years, mean 16 years) over a 2-year period. 413 cervical specimens were obtained and analyzed cytologically and by a southern blot (sb) method for hpv dna. 277 specimens from 210 patients could be fully analyzed. 23 patients (10.9%) were positive for hpv dna by sb. cytologic findings in these 23 patients demonstrated changes compatible with low-grade squamous intr ... | 1993 | 8390646 |
| common warts from immunocompetent patients show the same distribution of human papillomavirus types as common warts from immunocompromised patients. | we studied the papillomaviruses (hpv) found in 131 common warts from 111 immunocompetent patients by amplification of viral dna sequences with the general-primer-mediated polymerase chain reaction (pcr). the virus types were determined by restriction-enzyme cleavage and reverse-blot analysis. results were confirmed by using the southern blot technique. forty patients harboured hpv 2a, 25 individuals showed hpv 2c and 13 yielded hpv 57. common warts from 16 patients were induced by a variant of h ... | 1993 | 8385983 |
| [riboprobes: an alternative in the detection of viral sequences]. | the use of riboprobes for the detection of rna viral sequences is analyzed. subgenomic fragments of cdna from poliovirus type 2, dengue virus type 4 and human immunodeficiency virus type 1, were inserted downstream sp6 and/or t7 promoters in the transcription vectors pgem-4z or psp64. rnas obtained by in vitro transcription in the presence of utp infinity (32p) were used as probes for the detection of rna viral sequences from infected cell lines in slot and northern blot assays. the poliovirus r ... | 1994 | 8073329 |
| [typification of a subgroup of cervical papilloma by in situ hybridization. preliminary study]. | the purpose of this study was to analyze 19 patients infected with human papilloma virus (hpv), in order to determine the most frequent subtype. subjects were patients of the national institute of perinatology (inper), between 1991-1992. all the patients had positive cytology, cervical biopsy was done through colposcopy control. sample characteristics were: mean age 29 years, married; most of them multiparous (9/19) and 7 were monogamous. six out of 12 cytologies were positive for hpv, 12 out of ... | 1994 | 8056363 |
| effect of oral aromatic retinoid (ro 10-9359) on human papilloma virus-2-induced common warts. | persisting human papilloma virus (hpv) 2 induced common warts of a chronic lymphatic leukemia patient were orally treated with aromatic retinoid ro 10-9359 (tigason). clinically, the lesions improved rapidly. virus-specific cytopathogenic effects (cpe), virus particles and viral dna were no longer detectable. therapy was discontinued because of the development of a liposarcoma, which led to a complete relapse of the cutaneous lesions. hpv-2 specific parameters, cpe and viral dna, were restored. ... | 1983 | 6840376 |
| identification of human papillomavirus dna in cervical and vaginal intraepithelial neoplasia with molecularly cloned virus-specific dna probes. | the presence of human papillomavirus (hpv) dna was identified in the tissues of cervical and vaginal intraepithelial neoplasia by southern blot dna hybridization under conditions of low stringency. the specific types of hpv present in the tissues were identified by using molecularly cloned types 1 through 6 (hpv-1 through hpv-6) hpv dna probes under high-stringency conditions. all tissues of cervical and vaginal intraepithelial neoplasia analyzed contained hpv genomes. fifteen of 19 samples (79% ... | 1983 | 6313534 |
| structural proteins of poliovirus type 2 isolates. | seventeen strains of type 2 poliovirus isolated from vaccines and vaccine-contacts in japan were analyzed for viral structural proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis. an alteration in electrophoretic mobility of vp1 was observed in most of the strains from paralytic patients and healthy vaccinees. the alteration in vp1 was observed at a high frequency among many strains, but no significant relation between the mobility change of vp1 and antigenic marker or rct/40 m ... | 1981 | 6268945 |
| anogenital warts contain several distinct species of human papillomavirus. | anogenital warts from 26 patients were examined for the presence of human papillomavirus (hpv). although no whole, intact virus could be identified, varying amounts of nonintegrated hpv dna were detected in 18 tissue specimens (70%) by employing both an agarose gel-ethidium bromide staining method and the southern blot hybridization procedure. when hybridization analysis was performed under stringent conditions, six anogenital warts were observed to contain hpv genomic sequences related to eithe ... | 1980 | 6255208 |
| molecular analysis of the effect of topical imiquimod treatment of hpv 2/27/57-induced common warts. | imiquimod is effective in the treatment of genital warts and clinical studies suggest activity against common warts as well. we have analyzed the effect of topical imiquimod on gene expression and virus load in human papilloma virus (hpv) 2/27/57-induced common warts. mrna was extracted from keratinocyte culture, from normal skin, from three untreated common warts and from three common warts treated topically with 5% imiquimod cream twice daily. differential gene expression was demonstrated by r ... | 2004 | 15452412 |
| antiviral evaluation of plants from brazilian atlantic tropical forest. | the antiviral activity of six medicinal plants from brazilian atlantic tropical forest was investigated against two viruses: herpes simplex virus type 1 (hsv-1) and poliovirus type 2 (pv-2). cuphea carthagenensis and tillandsia usneoides extracts showed the best antiherpes activity. t. usneoides dichloromethane, ethyl acetate and n-butanol extracts, and lippia alba n-butanol extract showed inhibition of hsv-1, strain 29r/acyclovir resistant. in addition, only l. alba ethyl acetate extract showed ... | 2005 | 15890472 |
| inhibition of dna replication of human papillomavirus by artificial zinc finger proteins. | recently, we demonstrated that plant dna virus replication was inhibited in planta by using an artificial zinc finger protein (azp) and created azp-based transgenic plants resistant to dna virus infection. here we apply the azp technology to the inhibition of replication of a mammalian dna virus, human papillomavirus type 18 (hpv-18). two azps, designated azp(hpv)-1 and azp(hpv)-2, were designed by using our nondegenerate recognition code table and were constructed to block binding of the hpv-18 ... | 2006 | 16699021 |
| human papilloma virus persistence and neopterin, folate and homocysteine levels in cervical dysplasias. | objective: to investigate the relationship between human papilloma virus (hpv) infection persistence and serum folate, vitamin b12, homocysteine, neopterin levels in cervical dysplasia. study design: through the years 2007-2008, 122 women who have admitted to gynecology clinic were evaluated for cervical cytology, demographical characteristics, hpv infection, serum folate, vitamin b12, homocysteine, albumin, and neopterin levels. results: considering all the cases, the highest percentage of the ... | 2010 | 20740364 |