| inverted repetitive sequences in human papilloma virus 1 (hpv-1) dna. | | 1977 | 201085 |
| seroepidemiologic studies of bovine papillomavirus infections. | bovine and human sera were analyzed for the presence of antibodies against bovine papillomavirus types 1 and 2 (bpv 1 and 2) and human papillomavirus type 1 (hpv 1) in a solid-phase radioimmunoassay. human sera did not react with bpv antigens, and bovine sera showed no evidence of antibodies against hpv 1. in contrast, 19% of all bovine sera tested reacted with bpv 1 and 2 antigens, and 35% of human sera revealed antibodies against hpv 1. no serologic evidence was obtained for heterologous infec ... | 1979 | 220453 |
| localization of viral dna-replication in sections of human warts by nucleic acid hybridization with complementary rna of human papilloma virus type 1. | using complementary rna of human papilloma virus type 1 (hpv1) and in situ hybridization techniques the localization of viral dna replication was studied in sections of 38 human virus acanthomata from 31 different patients. in five cases significant labeling was detected by autoradiography. labeling started always in the first or the second suprabasal cell layer and was strongly limited to the nuclei. a remarkable early beginning of the vacuolated process seemed to be correlated with the visible ... | 1979 | 220921 |
| characterization of human papillomavirus 3 in warts of a renal allograft patient. | multiple flat wart-like lesions of a renal allograft recipient were shown to contain hpv 3 or a serologically crossreacting virus by indirect immunofluorescence with monospecific animal antisera against hpv [1--5]. the patient's serum revealed virus specific antibodies (immunofluorescence titer 1/80). papillomaviruses were isolated and after in vitro iodination 3 major proteins (mw 70.000, 56.000 and 43.000) were detected by sds polyacryalmide gel electrophoresis. dna was extracted from the wart ... | 1979 | 227966 |
| factors involved in enzyme-linked immunoassay of viruses and evaluation of the method for identification of enteroviruses. | a quantitative enzyme-linked immunosorbent assay was used for identification of selected enteroviruses: poliovirus type 1, echovirus type 6, coxsackievirus a type 9, and coxsackievirus b types 1 through 6. partially purified viral antigens or virus-specific antibodies were adsorbed to polystyrene spectrophotometer cuvettes, which permitted the assays to be reported and compared in terms of enzyme units specifically reacting. both the adsorbed antigen and the adsorbed antibody methods were approx ... | 1979 | 229122 |
| human papillomavirus type 1 e4 proteins differing by their n-terminal ends have distinct cellular localizations when transiently expressed in vitro. | two major human papillomavirus type 1 (hpv-1) e4 proteins are found in large amounts in productively infected differentiating wart cells, a 17-kda protein translated from an e1-e4 transcript and a processed 16-kda protein lacking the e1 amino acids at least. the functions of the e4 proteins are still unknown. we have designed an in vitro system allowing the transient expression of three forms of hpv-1 e4 proteins: the 17-kda e1-e4 protein, an e4 protein without the five e1 amino acids (e4-3200), ... | 1992 | 1309915 |
| cytotoxic and viral neutralizing antibodies crossreact with streptococcal m protein, enteroviruses, and human cardiac myosin. | the development of autoimmunity in certain instances is related to infectious agents. in this report, cytotoxic monoclonal antibodies (mabs) that recognize epitopes on both enteroviruses and the bacterium streptococcus pyogenes are described. murine anti-streptococcal mabs that were crossreactive with streptococcal m protein, human cardiac myosin, and other alpha-helical coiled-coil molecules were found to neutralize coxsackieviruses b3 and b4 or poliovirus type 1. the viral-neutralizing anti-st ... | 1992 | 1311095 |
| poliovirus chimeras expressing sequences from the principal neutralization domain of human immunodeficiency virus type 1. | sequences from the principal neutralization domain of human immunodeficiency virus type 1 (hiv-1) strain lai or rf have been expressed in antigenic site 1 of the capsid of the sabin strain of poliovirus type 1. a number of the resulting chimeras were viable. viable variants bearing mutations within the insertion site spontaneously arose from several nonviable chimeras. in general, these mutations result in a decrease in positive charge in the substituted antigenic site 1. two of the chimeras wer ... | 1992 | 1373205 |
| antibodies may act synergistically or additively with interferon to inhibit poliovirus. | we investigated the protective effects of combinations of interferon-alpha (ifn-alpha) and neutralizing monoclonal antibodies (mabs) and serum antibodies against poliovirus type 1 (pv-1) in vitro. our results indicate that the antiviral effects of ifn-alpha and most neutralizing mabs to pv-1 act synergistically to inhibit pv-1. however, the antiviral effects of ifn-alpha and one type specific mab to pv-1 were additive. further, the protective effects observed with combinations of ifn-alpha and r ... | 1991 | 1665535 |
| humoral assays of human sera to disrupted and nondisrupted epitopes of human papillomavirus type 1. | the use of different assay systems and the disparity in results obtained has meant that we have little understanding about the role played by the humoral response during human papillomavirus (hpv) infection. human antibody responses have so far appeared to be largely directed against the major capsid protein, l1. this protein possesses both type-specific and type-common antigenic determinants but it is not known which of these is important in vivo during the natural course of infection. in this ... | 1990 | 1689524 |
| specific detection of enteroviruses in clinical samples by molecular hybridization using poliovirus subgenomic riboprobes. | enteroviruses were specifically detected in crude clinical specimens or in cell cultures in which the viruses were amplified by dot hybridization by using poliovirus type 1-derived, subgenomic radiolabeled crna probes (riboprobes). the sensitivity of this test varied from 2.5 to 33%, when clinical specimens without cell culture were examined, and was about 85% in cell culture lysates. the specificity of the test was 90 to 100%. the riboprobe corresponding to the 5'-noncoding sequence specificall ... | 1990 | 2155917 |
| integrated hpv 1 genomes in a human keratinocyte cell line can be transactivated by a sv40/bpv1 recombinant virus which expresses bpv1 e2 proteins. | this paper describes studies carried out on an hpv 1 carrying human keratinocyte cell line (svd2) and two subclones of it. although these lines contain multiple copies of hpv 1 genomes, in situ hybridization revealed that integration was restricted to band q33 on the long arms of chromosome 2. an e4 1.25-kb mrna was specifically identified by northern blotting and a pcr generated cdna confirmed the presence of the e1/e4 spliced mrna which is abundant in hpv 1 containing papillomas. infection of ... | 1990 | 2158183 |
| characterization of poliovirus clones containing lethal and nonlethal mutations in the genome-linked protein vpg. | viral rna synthesis was assayed in hela cells transfected with nonviable poliovirus rna mutated in the genome-linked protein vpg-coding region. the transfecting rna was transcribed in vitro from full-length poliovirus type 1 (mahoney) cdna containing a vpg mutagenesis cartridge. hybridization experiments using ribonucleotide probes specific for the 3' end of positive- and negative-sense poliovirus rna indicated that all mutant rnas encoding a linking tyrosine in position 3 or 4 of vpg were repli ... | 1990 | 2159557 |
| evidence for a novel picornavirus in human dermatomyositis. | we prepared rna probes from cloned segments of human and murine enteroviruses (evs) for in situ hybridization of skeletal muscle biopsies from patients with dermatomyositis (dm), polymyositis, other inflammatory myopathies, and noninflammatory muscle diseases, and from normal control subjects. a probe derived from theiler's murine encephalomyelitis virus (tmev) detected viral rna within mononuclear cells of the interstitial connective tissue in 3 of 5 patients with adult-onset dm. none of these ... | 1989 | 2549850 |
| enterovirus replication in porcine ileal explants. | organ explants of porcine ileum were cultured in different media for up to 48 h. tissue preservation was evaluated by light microscopy and by transmission and scanning electron microscopy. cellular structure was well maintained after incubation for 48 h in cmrl-1066 supplemented with insulin and cortisone. explants of absorptive or lymphoid tissue from young or adult pigs were incubated with either coxsackievirus b5 (which is infectious for swine) or human poliovirus type 1 (which served as a co ... | 1987 | 2821187 |
| infectivity and antigenicity reduction rates of human rotavirus strain wa in fresh waters. | the rates of inactivation of human rotavirus type 2 (strain wa) (hrv-wa) and poliovirus type 1 (strain chat) were compared in polluted waters (creek water and secondary effluent before chlorination) and nonpolluted waters (lake water, groundwater, and chlorinated tap water). viral infectivity titers were determined by plaque assays, while hrv-wa antigenicity also was monitored by an enzyme-linked immunosorbent assay. both viruses persisted longest in lake water and shortest in tap water. the act ... | 1987 | 2821902 |
| humoral and cellular immunity to papillomavirus in patients with cervical dysplasia. | the cell-mediated and humoral immune responses to human papillomavirus (hpv) were tested in groups of patients with various degrees of cervical intraepithelial neoplasia (cin) using a lymphocyte proliferation assay (lpa) as a measure of circulating sensitised t-cells and an enzyme-linked immunosorbent assay (elisa) for antibodies. twenty-three of 92 patients (25%) gave stimulation indices (s.i.) greater than two to at least one of the several antigen preparations tested in the lpa. of 282 patien ... | 1988 | 2828535 |
| intertypic cross-neutralization of polioviruses by human monoclonal antibodies. | human b cell lines producing monoclonal antibodies (mabs) reactive with poliovirus type 1 were generated by transformation with epstein-barr virus (ebv) b 95-8 of tonsillar lymphocytes from several immune donors. ebv-transformed cells were cloned in semisolid agarose. some neutralizing (nt) human mabs recognized and neutralized only poliovirus type 1, whereas other nt mabs neutralized either poliovirus type 1 and 2 or all three serotypes. mabs reactive with poliovirus type 1 and 3 but not with t ... | 1988 | 2831657 |
| a highly efficient second-step concentration technique for bacteriophages and enteric viruses using ammonium sulfate and tween 80. | addition of tween 80 to a 1.5% solution of beef extract was found to enhance the elution of bacteriophages adsorbed to electronegative filters. when reconcentration of the eluate was attempted by ammonium sulfate precipitation, a floating layer containing most of the viruses was formed. this floating layer can be obtained with several nonionic detergents including tween 80 and under a salt saturation of 55% with ammonium sulfate, potassium tartrate, and sodium phosphate. virus recovery ranged fr ... | 1988 | 2850100 |
| use of bituminous coal as an alternative technique for field concentration of waterborne viruses. | a filter system that sandwiches a bituminous coal preparation between two prefilters was comparable to those presently used to recover human viruses from large volumes of water. this filter was effective over a ph range of 3.0 to 7.0. poliovirus type 1 recoveries from 100-liter seeded samples of cincinnati tap water did not vary significantly when compared with those of identical samples processed through filterite and millipore filters. in studies with raw domestic sewage, virus recoveries were ... | 1985 | 4004235 |
| ultraviolet devitalization of eight selected enteric viruses in estuarine water. | the effect of ultraviolet (uv) radiation on the devitalization of eight selected enteric viruses suspended in estuarine water was determined. the surviving fractions of each virus were calculated and then plotted against the uv exposure time for purposes of comparison. analytical assessment of the survival data for each virus consisted of least squares regression analysis for determination of intercepts and slope functions. all data were examined for statistical significance. when the slope func ... | 1970 | 4316273 |
| human papilloma virus type i purified from human genital warts. | human wart virus (hpv) was isolated from a pool of genital warts. the electrophoretic mobility of virion proteins was studied by sds polyacrylamide gel electrophoresis and showed the same pattern as that obtained with hpv-1. the analysis of dna after restriction enzyme digestion with the endonucleases hind iii and hae iii, and nucleic acid hybridization did not show any difference with hpv-1. the viral particles were agglutinated by anti-hpv-1 serum, as shown by electron microscopic particle agg ... | 1981 | 6266889 |
| identification of the human papilloma virus-1a e4 gene products. | antibodies prepared against a human papilloma virus-1 (hpv-1) e4/beta-galactosidase fusion protein identified several polypeptides in hpv-1, but not hpv-2 or 4, induced papillomas. the major e4 protein, that represented up to 30% of total cellular protein, was a 16/17-k doublet which was purified by column chromatography and analysed for amino acid content. a peptide derived by chymotryptic digestion was purified by h.p.l.c. and subjected to amino acid sequencing. the unique sequence obtained, g ... | 1986 | 3011404 |
| a virion concentration method for detection of human enteric viruses in oysters by pcr and oligoprobe hybridization. | this article reports the development of a method to purify and concentrate intact virions from oyster extracts to volume and quality compatible with viral genomic nucleic acid amplification by reverse transcriptase pcr (rt-pcr) and confirmation by oligonucleotide probe hybridization. fifty-gram oyster samples were processed by an adsorption-elution -precipitation method and then seeded with 10(1) to 10(5) pfu of poliovirus type 1 (pv1) or hepatitis a virus (hav). seeded viruses in oyster extract ... | 1996 | 8787405 |
| human enterovirus infection in stray dogs. some aspects of interest to public health. | to investigate the possible role of domestic animals as reservoirs of human enteroviruses, we studied 212 stray dogs captured in different areas of the municipality of são paulo. the captured animals were divided into 19 groups of 10 to 20 dogs each; faeces of 126 of the 212 dogs were processed for enterovirus isolation. the following viruses were isolated from 12 dogs; poliovirus type 1 (2 dogs), poliovirus type 3 (1 dog), echovirus type 7 (8 dogs) and echovirus type 15 (1 dog). of the 12 infec ... | 1996 | 9071037 |
| detection and localization of human papillomavirus dna in human genital condylomas by in situ hybridization with biotinylated probes. | we have examined the distribution of human papillomavirus (hpv) dna in paraffin sections of humans warts by in situ hybridization with biotin-labeled dna probes. recombinant plasmid dnas (hpv-1, -6, -11, -16) were labeled by nick translation with biotinylated deoxyuridine triphosphate. paraffin sections were hybridized with the probes for 18 h in stringent or non-stringent conditions, and dna-dna hybrids were detected by immunocytochemistry. paraffin sections of warts were also examined for the ... | 1985 | 2993496 |
| epidemiology of butchers' warts. | studies were carried out in two slaughter-houses in different cities differing in the degree of work automation and, for comparison, in workers of nearby factories of the same two cities. there was a high incidence of warts (49.2%) in a slaughterhouse where the workers had direct contact with animals and meat, while a significantly lower incidence (9%) was observed in a modern slaughterhouse where the work was almost completely automated. the types of human papillomaviruses (hpvs) were similar i ... | 1988 | 2841908 |
| distinct cellular receptor interactions in poliovirus and rhinoviruses. | receptor binding to human poliovirus type 1 (pv1/m) and the major group of human rhinoviruses (hrv) was studied comparatively to uncover the evolution of receptor recognition in picornaviruses. surface plas- mon resonance showed receptor binding to pv1/m with faster association and dissociation rates than to hrv3 and hrv16, two serotypes that have similar binding kinetics. the faster rate for receptor association to pv1/m suggested a relatively more accessible binding site. thermodynamics for re ... | 2000 | 10716921 |
| immunomodulatory therapy in the management of viral infections in patients with hiv infection. | human immunodeficiency virus (hiv) causes disease by infecting lymphocytes and progressively destroying critical regulatory and effector cells of the immune system, leaving patients vulnerable to a number of bacterial, fungal, and viral infections. facial herpes (herpes simplex virus-1 [hsv-1]), genital herpes (hsv-2), herpes zoster (varicella zoster virus), oral hairy leukoplakia (epstein-barr virus), kaposi's sarcoma (hhv-8), molluscum contagiosum, condyloma acuminata (human papillomavirus [hp ... | 2000 | 10861104 |
| enterovirus-induced production of pro-inflammatory and t-helper cytokines by human leukocytes. | enteroviruses are associated with chronic inflammatory and autoimmune diseases in humans. in these conditions, the cytokine network is supposed to have an important role in inflammation and modulation of the (auto)immune response. in the present study, we demonstrate that coxsackie virus b4 and poliovirus type 1 induce production of pro-inflammatory cytokines il-1 beta and tnf-alpha in freshly isolated human leucocytes. furthermore, enteroviruses stimulate the production of cytokines belonging t ... | 2000 | 11097750 |
| analysis of hpv-1 e4 gene expression using epitope-defined antibodies. | six monoclonal antibodies (mabs) have been raised against the e4 proteins of hpv-1. five of these were found to recognize denaturation-resistant epitopes as determined by western blotting--and their binding sites were identified by determining their reactivity against a panel of bacterial e4--beta-galactosidase fusion proteins which contained progressive deletions at the c-terminal end of the e4 region. the five mabs were found to bind to four distinct sites. by using these epitope-defined mabs, ... | 1988 | 2456213 |
| transgenic mice expressing a human poliovirus receptor: a new model for poliomyelitis. | a human poliovirus receptor (pvr) gene was used to generate transgenic mice that express pvr transcripts and poliovirus binding sites in a wide range of tissues. intracerebral inoculation of pvr transgenic mice with poliovirus type 1, mahoney strain, resulted in viral replication in the brain and spinal cord and development of paralytic poliomyelitis. p1/mahoney did not replicate or cause paralysis in nontransgenic mice. pvr transgenic mice failed to develop clinical disease when inoculated intr ... | 1990 | 2170026 |
| study of stringency conditions for human papillomavirus dna detection on cell lines, frozen and paraffin-embedded tissue sections by in situ hybridization with biotinylated probes. | in situ hybridization was mainly used for typing human papillomavirus (hpv) in paraffin-embedded or frozen sections under stringent conditions (sc). we tested 5 different conditions of stringency with biotinylated hpv 1, 2, 16 and 18 probes on 3 cell lines (sihà and caski with hpv16, hela with hpv18) by varying the concentration of formamide in the hybridization mixture and washings in order to determine the stringency conditions to be used to assess the presence of hpv and its typing: a-low str ... | 1990 | 2158491 |
| presence and type specificity of papillomavirus antibodies demonstrable by immunoelectron microscopy tests in samples from patients with warts. | sera from 75 patients with warts were tested for antibodies against human papillomaviruses (hpvs) in an immunoelectron microscopy test. a group of 40 patients' sera were tested against a pooled suspension of the hpvs extracted from their warts and 90% of the sera were found to be antibody-positive. prior to serological investigation viruses from a second group of 35 patients were typed by dot blot hybridization with hpv-1 and hpv-2 dna. according to these results the patients were divided into t ... | 1990 | 2155296 |
| presence of human immunodeficiency virus nucleic acids in wastewater and their detection by polymerase chain reaction. | the human immunodeficiency virus type 1 (hiv-1) released by infected individuals or present in human and hospital wastes can potentially cause contamination problems. the presence of hiv-1 was investigated in 16 environmental samples, including raw wastewater, sludge, final effluent, soil, and pond water, collected from different locations. a method was developed to extract total nucleic acids in intact form directly from the raw samples or from the viral concentrates of the raw samples. the iso ... | 1992 | 1476440 |
| inhibition of dna replication of human papillomavirus by artificial zinc finger proteins. | recently, we demonstrated that plant dna virus replication was inhibited in planta by using an artificial zinc finger protein (azp) and created azp-based transgenic plants resistant to dna virus infection. here we apply the azp technology to the inhibition of replication of a mammalian dna virus, human papillomavirus type 18 (hpv-18). two azps, designated azp(hpv)-1 and azp(hpv)-2, were designed by using our nondegenerate recognition code table and were constructed to block binding of the hpv-18 ... | 2006 | 16699021 |
| comoviruses and enteroviruses share a t cell epitope. | an in vitro murine t cell proliferation assay was used to determine whether an antigenic epitope(s) recognized by enterovirus-immune t cells is held in common between plant comoviruses and human enteroviruses. splenocytes isolated from c3h/hej mice infected with coxsackievirus b3 (cvb3) proliferated in vitro not only against a variety of enterovirus (cvb2, cvb3, cvb6, cva16, pv1) antigens, but against comovirus (cpmv, bpmv) antigens as well. splenocytes from mice inoculated with bean pod mottle ... | 1992 | 1370127 |
| in vitro infection of normal human keratinocytes by human papillomavirus type 1 followed by amplification of the viral genome in reconstructed epidermis. | primary cultures of normal human keratinocytes were inoculated in vitro with human papillomavirus type 1 (hpv-1), the agent responsible for deep plantar warts. upon transfer to dead de-epidermized dermis and growth at the air-liquid interface, keratinocytes reconstituted a pseudoepidermis. under these highly differentiating conditions, hpv-1 dna amplification was found to take place in the reconstructed epidermis, being detectable from 7 days after the transfer and persisting for at least 10 day ... | 1992 | 1335028 |
| antiviral activity and mode of action of a peptide isolated from sorghum bicolor. | in this paper, we describe the purification of an antiviral peptide from seeds of sorghum bicolor l. by a procedure that included gel filtration, ion exchange, and high-performance liquid chromatography (hplc) in a reversed-phase column. its molecular weight, determined by chromatographic mobility on the shim-pack diol-150 gel permeation column in hplc, was found to be 2000da. the peptide designated 2kd peptide strongly inhibited the replication of herpes simplex virus type 1 (hsv-1), dose-depen ... | 2008 | 17890069 |
| detection of antibodies to the e4 or e7 proteins of human papillomaviruses (hpv) in human sera by western blot analysis: type-specific reaction of anti-hpv 16 antibodies. | to determine the cross-reactivity between early (e) proteins of different human papillomavirus (hpv) types, 346 serum samples were tested with e4 and e7 of hpv 16. two hundred and sixteen of them were also tested with hpv 1 e4, 21 with hpv 11 e4 and e7, and 109 with hpv 18 e4 and e7. viral fusion proteins were expressed in escherichia coli and used as antigens in western blot experiments. the sera were obtained from patients with hpv-associated genital lesions or cervical cancer, from renal tran ... | 1992 | 1326715 |
| a host-specific, temperature sensitive translation defect determines the attenuation phenotype of a human rhinovirus/poliovirus chimera pv1(ripo). | using pv1(ripo), a chimeric poliovirus, utilizing the cognate ires of human rhinovirus type 2 (hrv2), we set out to shed light on the mechanism by which this variant expresses its attenuated phenotype in poliovirus-sensitive, cd155 transgenic (tg) mice and cynomolgus monkeys. here we report that replication of pv1(ripo) is restricted not only in human cells of neuronal origin, as was reported previously, but also in cells of murine origin at physiological temperature. this block in replication w ... | 2011 | 21561914 |
| mouse adaptation determinants of poliovirus type 1 enhance viral uncoating. | most poliovirus (pv) strains, such as pv type 1/mahoney, cannot infect the mouse central nervous system. we previously identified two determinants of mouse adaptation of pv type 1/mahoney at positions 22 and 31 of the viral capsid proteins vp1 and vp2, respectively (t. couderc, j. hogle, h. le blay, f. horaud, and b. blondel, j. virol. 67:3808-3817, 1993). these residues are located on the interior surface of the capsid. in an attempt to understand the molecular mechanisms of adaptation of pv to ... | 1996 | 8523541 |
| comparative study on e6 and e7 genes of some cutaneous and genital papillomaviruses of human origin for their ability to transform 3y1 cells. | e6 (or e7) genes of some genital and cutaneous human papillomaviruses (hpvs) were compared for their ability to transform cells of a rat fibroblastic line (3y1) by using recombinant retroviruses. the e6 gene of genital cancer-associated hpv 16 or 18 was found to induce a characteristic morphological change, i.e., densely packed arrays of elongated cells forming swirl patterns. this change is the same as that induced by the e6 gene of cutaneous cancer-associated hpv 5, 8, or 47, which we describe ... | 1993 | 8390746 |
| common warts from immunocompetent patients show the same distribution of human papillomavirus types as common warts from immunocompromised patients. | we studied the papillomaviruses (hpv) found in 131 common warts from 111 immunocompetent patients by amplification of viral dna sequences with the general-primer-mediated polymerase chain reaction (pcr). the virus types were determined by restriction-enzyme cleavage and reverse-blot analysis. results were confirmed by using the southern blot technique. forty patients harboured hpv 2a, 25 individuals showed hpv 2c and 13 yielded hpv 57. common warts from 16 patients were induced by a variant of h ... | 1993 | 8385983 |
| isolation and structure of woodorien, a new glucoside having antiviral activity, from woodwardia orientalis. | hot aqueous and methanol extracts of the rhizomes of woodwardia orientalis were tested for their in vitro antiviral activity against herpes simplex virus type 1 (hsv-1), poliovirus type 1, and measles virus by plaque reduction assay. the aqueous extract of w. orientalis reduced the plaque forming ability of hsv-1 and poliovirus more strongly than did the methanol extract. by bioassay-directed fractionation of the aqueous extract, a new glucoside, woodorien (1), along with five known compounds we ... | 1993 | 8281578 |
| antiviral traditional medicines against herpes simplex virus (hsv-1), poliovirus, and measles virus in vitro and their therapeutic efficacies for hsv-1 infection in mice. | one hundred forty-two kinds of traditional medicines, which have been historically used in china, indonesia, and japan, were examined for the antiviral activity of their hot water (hw) extracts against herpes simplex virus type 1 (hsv-1), poliovirus type 1, and measles virus by plaque reduction assay. thirty-two, 55, and 30 hw-extracts of them showed anti-hsv-1, antipoliovirus, and anti-measles virus activities, respectively. among the 32 hw-extracts with anti-hsv-1 activity, 3 hw-extracts had a ... | 1993 | 8279811 |
| double infection with human papillomavirus 1 and human papillomavirus 63 in single cells of a lesion displaying only an human papillomavirus 63-induced cytopathogenic effect. | specific histologic features are associated with the presence of certain human papillomaviruses (hpv) in verrucous lesions of the skin. hpv 1 is known to be associated with eosinophilic keratohyalin-like intracytoplasmic inclusion bodies, granular in appearance, whereas the recently described hpv 63 seems to induce inclusion bodies displaying a filamentous structure. although an infection by two distinct hpv types within a single cell has not been previously reported, we have recently treated a ... | 1993 | 8246450 |
| detection of adenoviruses and enteroviruses in polluted waters by nested pcr amplification. | a procedure has been developed for the rapid detection of enteroviruses and adenoviruses in environmental samples. several systems for virus concentration and extraction of nucleic acid were tested by adding adenovirus type 2 and poliovirus type 1 to different sewage samples. the most promising method for virus recovery involved the concentration of viruses by centrifugation and elution of the virus pellets by treatment with 0.25 n glycine buffer, ph 9.5. nucleic acid extraction by adsorption of ... | 1994 | 8085832 |
| the human poliovirus receptor. receptor-virus interaction and parameters of disease specificity. | the host range of poliovirus is determined by the expression of the hpvr, a member of the immunoglobulin superfamily. we characterized hpvr proteins biochemically and found them to be complex-type glycoproteins. the outermost v-like domain of three extracellular domains harbors the pvr function. a panel of single or multiple amino acid exchanges were introduced throughout this domain in order to localize regions involved in virus-receptor interactions. putative contact amino acids were found to ... | 1995 | 7611627 |
| a new cis-acting element for rna replication within the 5' noncoding region of poliovirus type 1 rna. | mouse cells expressing the human poliovirus receptor (pvr-mouse cells) as well as human hela cells are susceptible to poliovirus type 1 mahoney strains and produce a large amount of progeny virus at 37 degrees c. however, the virus yield is markedly reduced at 40 degrees c in pvr-mouse cells but not in hela cells. the reduction in virus yield at 40 degrees c appears to be due to a defective initiation process in positive-strand rna synthesis (k. shiroki, h. kato, s. koike, t. odaka, and a. nomot ... | 1995 | 7474095 |
| identification of human papillomavirus dna in cervical and vaginal intraepithelial neoplasia with molecularly cloned virus-specific dna probes. | the presence of human papillomavirus (hpv) dna was identified in the tissues of cervical and vaginal intraepithelial neoplasia by southern blot dna hybridization under conditions of low stringency. the specific types of hpv present in the tissues were identified by using molecularly cloned types 1 through 6 (hpv-1 through hpv-6) hpv dna probes under high-stringency conditions. all tissues of cervical and vaginal intraepithelial neoplasia analyzed contained hpv genomes. fifteen of 19 samples (79% ... | 1983 | 6313534 |
| failure of guanidine and 2-(alpha-hydroxybenzyl)benzimidazole to inhibit replication of hepatitis a virus in vitro. | replication of hepatitis a virus (hav) in the human hepatoma-derived plc/prf/5 cell line was neither inhibited in the presence of various concentrations of guanidine or d-2-(alpha-hydroxybenzyl)benzimidazole (d-hbb), nor were the two chemicals effective in combination. under identical conditions, however, replication of poliovirus type 1 was inhibited. tracer experiments with radiolabelled guanidine and d-hbb also furnished no evidence that the two antiviral substances were metabolized gradually ... | 1982 | 6288846 |
| analysis of human genital warts (condylomata acuminata) and other genital tumors for human papillomavirus type 6 dna. | 32p-labelled cloned hpv 6 dna was used as probe to analyze human genital tumors for dna sequences homologous to hpv 6 dna. ninety three percent of all condylomata acuminata (41 out of 44) were found to harbor hpv 6 dna. of the remaining three, one contained hpv 1 dna. no papillomavirus dna was identified in the two other tumors. all three invasively growing giant condylomata acuminata (buschke-löwenstein tumors) investigated also contained hpv 6 dna. two out of six atypical condylomata of the ce ... | 1982 | 6277807 |
| anogenital warts contain several distinct species of human papillomavirus. | anogenital warts from 26 patients were examined for the presence of human papillomavirus (hpv). although no whole, intact virus could be identified, varying amounts of nonintegrated hpv dna were detected in 18 tissue specimens (70%) by employing both an agarose gel-ethidium bromide staining method and the southern blot hybridization procedure. when hybridization analysis was performed under stringent conditions, six anogenital warts were observed to contain hpv genomic sequences related to eithe ... | 1980 | 6255208 |
| [cervical intraepithelial neoplasia (cin) and human papillomavirus (hpv) infection]. | many studies have shown a strong correlation between cin and hpv infection. molecular biology has allowed identification of types of hpv which seem to be connected, more frequently than others, to dysplastic lesions. physical state of hpv-genome seems to play an important role in the development of cervical cancer. in this study the hpv-genome has been searched in tissue specimens obtained from 34 women affected by cin ii and iii. all patients underwent laser conization. immediately before treat ... | 1997 | 9162879 |
| isolation and characterization of an anti-hsv polysaccharide from prunella vulgaris. | a water soluble substance was isolated from a chinese herb, prunella vulgaris, by hot water extraction, ethanol precipitation and gel permeation column chromatography. chemical tests showed that the substance was an anionic polysaccharide. using a plaque reduction assay, the polysaccharide at 100 microg/ml was active against the herpes simplex virus types 1 and 2 (hsv-1 and hsv-2), but was inactive against cytomegalovirus, the human influenza virus types a and b, the poliovirus type 1 or the ves ... | 1999 | 10588332 |
| purification of a hela cell receptor protein for group b coxsackieviruses. | coxsackievirus b3 (cb3) firmly attaches to hela cells, forming a specific complex between the virus and its receptor on the cell surface. we extracted this virus-receptor complex (vrc) with the detergents sodium deoxycholate and triton x-100. the vrc was identified by its sedimentation coefficient (140s), which was less than that of virions (155s). formation of the vrc from cell lysates and 3h-cb3 occurred with the same specificity as did attachment of virions to cells, in that its formation was ... | 1985 | 2991580 |
| host selenium status selectively influences susceptibility to experimental viral myocarditis. | the purpose of the present work was to determine whether dietary selenium (se) deficiency could influence the injurious effect of human viruses other than coxsackie virus b3 (cvb3) on mouse heart. weanling c3h/hen mice were fed a se-deficient or se-adequate diet for 4 wk and then were inoculated intraperitoneally with one of the following viruses: coxsackie virus b1 (cvb1), echovirus 9 (ev9), coxsackie virus a9 (cva9), or herpes simplex 1 (hsv1). polio virus 1 (pv1) was employed as a negative co ... | 2001 | 11393307 |
| regulation of human papillomavirus type 1 replication by the viral e2 protein. | the e1 and e2 proteins encoded by papillomaviruses are required for viral replication. earlier studies have shown that the viral e2 protein plays an important role in replication by targeting the e1 helicase to the origin of replication (ori). we have previously shown that the e1 protein of human papillomavirus (hpv) type 1 is sufficient for the in vivo replication of ori plasmids, although the e2 protein stimulates replication. in this study, we have further analyzed the role of the e2 protein ... | 2001 | 11504556 |
| ultraviolet inactivation of feline calicivirus, human enteric viruses and coliphages. | norwalk and norwalk-like viruses (nlv) are major causes of food- and water-related disease in the united states. there is no host cell line in which the nlv can be tested for infectivity. feline calicivirus (fcv) and nlv both belong to the family caliciviridae. fcv can be assayed for infectivity in the crandell reese feline kidney cell line, so fcv serves as a surrogate for nlv. this study is the first report of uv inactivation of fcv and also of using the plaque technique, in contrast to the 50 ... | 2002 | 12405148 |
| analysis of the cloverleaf element in a human rhinovirus type 14/poliovirus chimera: correlation of subdomain d structure, ternary protein complex formation and virus replication. | rna genomes of enteroviruses and rhinoviruses contain a 5'-terminal structure, the cloverleaf (cl), which serves as signal in rna synthesis. substitution of the poliovirus [pv1(m)] cl with that of human rhinovirus type 2 (hrv2) was shown previously to produce a viable chimeric pv, whereas substitution with the hrv14 cl produced a null phenotype. fittingly, the hrv14 cl failed to form a complex with pv-specific proteins 3cd(pro)-3ab or 3cd(pro)-pcbp2, considered essential for rna synthesis. it wa ... | 2003 | 12867653 |
| synergistic drug combinations against the in vitro replication of coxsackie b1 virus. | the existence of synergistic drug combinations against the in vitro replication of poliovirus type 1 (mahoney) (pv-1) had been established in our previous work. the objective of the present study was to test the combined effects of the different drugs against another representative of the enterovirus genus, i.e. coxsackievirus b1 (cbv-1). dual combinations of enviroxime, disoxaril, arildone, ptu-23, hbb and s-7 were evaluated. the susceptibility of cbv-1 to the individual effects of the inhibito ... | 2004 | 15026197 |
| nucleotide sequences of ires domains iv and v of natural echo virus type 11 isolates with different replicative capacity phenotypes. | echo viruses (ecv) belong to the enterovirus genus of the picornaviridae family and are the most frequently isolated from clinical and environmental samples. they are responsible for a wide variety of clinical syndromes involving most organs of the human body. we previously postulated that some of the variations in the recognition of echo virus type 11 (ecv 11) strains by a group specific monoclonal antibody (mab) which we have studied could be explained by variations in their replicative capaci ... | 2006 | 16732479 |
| evidence for emergence of diverse polioviruses from c-cluster coxsackie a viruses and implications for global poliovirus eradication. | the poliovirus (pv) eradication campaign is conducted on the premise that this virus, because of the lack of a zoonotic reservoir, will not reemerge once eradicated. this report examines the origin of pv using theoretical and experimental approaches. our rooted phylogenetic analysis suggests a speciation of pv from a c-cluster coxsackie a virus (c-cav) ancestor through mutation of the capsid that caused a receptor switch from intercellular adhesion molecule-1 to cd155, leading to a change of pat ... | 2007 | 17517601 |
| cytotoxic and antiviral activities of aporphine alkaloids of magnolia grandiflora l. | bioassay guided fractionation of the methanol extract of magnolia grandiflora l. (magnoliaceae) leaves led to the isolation and characterisation of four aporphine alkaloids, magnoflorine, lanuginosine, liriodenine and anonaine. the cytotoxicities of the pure compounds magnoflorine and lanuginosine were determined in a cell viability assay with the tumour cell lines hela (cervix tumour cell line), hepg2 (hepatocellular carcinoma cell line) and u251 (brain tumour cell line). magnoflorine was more ... | 2010 | 19764006 |