| tumorigenicity of human hematopoietic cell lines in athymic nude mice. | human hematopoietic cell lines, which had been classified on the basis of studies on clonality, and morphological, chromosomal and functional parameters as lymphoblastoid cell lines (lcl) of presumed non-neoplastic origin, and lymphoma, myeloma and leukemia lines of proven malignant origin, were tested for tumorigenic potential on subcutaneous transplantation to nude mice and for capacity to grow in semi-solid medium in vitro. recently established lcl failed to grow both in nude mice and in agar ... | 1977 | 14896 |
| appearance and some unusual features of epstein-barr virus antibody production in infectious mononucleosis and other health disorders. | the indirect immunofluorescence (if) test and the complement-fixation reaction (cfr) were used in examination of over 1500 sera obtained from patients with infectious mononucleosis (im) and other health disorders. the evidence obtained supports a direct aetiological relationship between epstein-barr virus (ebv) and im and points on a relationship of ebv to some other lymphadenopathies and health disorders. the incidence of the igg type antibody against virus capsid antigen (eb-vca) and soluble a ... | 1977 | 15437 |
| differential expression of d and r components of epstein-barr virus early antigen after superinfection and after induction with 5-iododeoxyuridine. | three non-productive human lymphoblastoid cell lines, nc-37, raji and l-1, either superinfected with epstein-barr (eb) virus from p3hr-1 cells or treated with 5-iododexyuridine (iudr), were studied for the development of early antigen (ea) of eb virus. in all three superinfected cell lines, both the restricted (r) and diffuse (d) componets of ea were synthesized, with r-component formation preceding d-component synthesis. after iudr treatment both components of ea were only formed in raji cells, ... | 1977 | 18915 |
| opportunistic non-hodgkin's lymphoma in x-linked recessive immunodeficiency and lymphoproliferative syndromes. | | 1977 | 21454 |
| epstein-barr virus infections in childhood. | igm and igg antibodies against epstein-barr virus (ebv) capsid antigen and antibodies against ebv nuclear antigen and heterophil antibodies were investigated in 115 paired sera of children with acute infections and in 100 sera of healthy controls of the same age and sex. ebv-specific igm antibodies could be recognized in 13.7% of the patients and in 7% of the controls. antibodies against ebv nuclear antigen were not detected in the igm-positive sera. | 1979 | 39436 |
| abortive growth of human lymphocytes carrying a dormant epstein-barr viral genome. | when p3hr-1 lymphoblastoid cells expressing epstein-barr virus (ebv)-related antigens at very low frequency were cocultivated with human umbilical cord blood lymphocytes and the cell-free mixed culture fluid was applied to fresh cord lymphocytes, cells morphologically distinct from normal lymphocytes became evident after one to two weeks' exposure. the abnormal cells became abundant after one month and were easily identified by b-cell markers and a variety of morphologic abnormalities. such abno ... | 1978 | 45600 |
| infectious mononucleosis: model for self-limiting lymphoproliferation. | evidence for the extensive lymphoproliferation which characterises infectious mononucleosis (i.m.) is summarised, and some of the mechanisms which are though to control lymphoproliferative activity in this disease are discussed. the main host responses which appear to limit lymphoproliferation in i.m. include the development of humoral antibodies against the epstein-barr (e.b.) virus-associated membrane antigen (present on the e.b. viral envelope and on e.b.-virus-infected cells) and cellular im ... | 1975 | 48066 |
| lymphoblastoid transformation and kinetics of appearance of viral nuclear antigen (ebna) in cord-blood lymphocytes infected by epstein-barr virus (ebv). | human cord-blood lymphocytes were infected with b95.8 epstein-barr virus (ebv) before and after separation into b- and t-cell populations. lymphoblastoid cells exhibiting b-cell characteristics appeared after 2 to 3 days of culture in the total population and in the separated b-cell subpopulation but not in the t-cell subpopulation. ebv nuclear antigen (ebna) was detected concurrently with the appearance of lymphoblastoid cells. the proportion of ebna-positive cells corresponded to that of lymph ... | 1975 | 49325 |
| critical evaluation of b and t cell markers in man. | | 1975 | 53874 |
| subpopulations of human lymphoblastoid cell lines. correlation with the expression of surface receptors and content of epstein-barr virus genome. | forty lymphoblast cell lines derived from normal subjects, patients with infectious mononucleosis, leukemia, and burkitt's lymphoma have been studied for surface receptors including surface ig, complement receptors by the eac rosette and fluorescent (raji cell) techniques, and fc (aggregate) receptor by direct and indirect immunofluorescence. because of the b-cell tropism of the epstein-barr virus (ebv), an effort was made to correlate the expresion of various surface properties of lymphoblastoi ... | 1976 | 62390 |
| detection of early antigens in nuclei of cells infected with cytomegalovirus or herpes simplex virus type 1 and 2 by anti-complement immunofluorescence, and use of a blocking assay to demonstrate their specificity. | skin fibroblasts exposed to cytosine arabinoside (ara c) were infected with either cytomegalovirus (cmv) or herpes simplex virus (hsv) type 1 and 2. herpesvirus-determined early antigens (hv-ea), detected by anti-complement immunofluorescence (acif), occurred primarily in the nucleic, and the specificity of these results was established by an acif blocking reaction using f(ab')2 fragments of human and hyperimmune reference sera. direct tests with selected sera and cross-blocking experiments betw ... | 1977 | 64461 |
| comparison of dna polymerase activities from an american burkitt's lymphoma cell line and ebv producer and nonproducer cells. | | 1975 | 65315 |
| comparative studies on ebv antigens by immunofluorescence and immunoperoxidase techniques. | three groups of ebv antigens, vca, ma and ea, were compared by the techniques of electron microscopic immunoperoxidase (ip) and immunofluorescence (if). p3hr-1 and ebv superinfected raji cells served as targets for labelled sera from patients with bl, npc and im or from healthy donors. 125i peroxidase-labelled antibodies were also prepared to determine, autoradiographically, the penetration of the complex into the cell system, and to monitor the incubation and washing procedures. the development ... | 1977 | 66208 |
| separation of the complement-fixing and early antigens from epstein-barr soluble antigen. | the epstein-barr virus-associated complement-fixing (soluble) antigen (cfsa) and the early antigen (ea) were isolated by ion-exchange chromatography from the p3hr-1 lymphoblastoid cell line activated with 5-iodo-2'-deoxyuridine for 72 hours. as expected, these two antigens were not immunologically identical. the cfsa, but not the ea, was isolated from the rajl lymphoblastoid cell line by the same method. | 1977 | 66320 |
| continuous lymphoblastoid suspension cultures from cells of haematopoietic organs of baboons with malignant lymphoma. report iii: immunological studies. | lymphoblastoid cultures established from haematopoietic organs of baboons with lymphoma belong to the b-cell type. cytoplasmic antigen is revealed in 2--6% of suspension culture cells established from lymphomatous baboons by indirect immunofluorescence reaction using serum from human burkitt's lymphoma patients with antibody titer to capsid antigen of epstein-barr virus 1: 320. there is a correlation between the number of antigen-containing cells in these cultures and the number of cells in whic ... | 1977 | 71245 |
| [virus and cancer]. | | 1977 | 71664 |
| detection of epstein-barr virus antigens with enzyme-conjugated antibody. | monospecific conjugated (fluorescein isothiocynate and horseradish peroxidase) goat antisera, prepared against three human immunoglobulin classes, im (mu), igg (gamma) and iga (alpha), were compared for their ability to detect human ig classes possessing specificity for epstein-barr virus (ebv) viral capsid antigens (vca) in a chronically infected human lymphoid cell line, p3j-hrik. it was determined that the enzyme system was significantly more sensitive than immunofluorescence in detecting mos ... | 1977 | 72045 |
| serum iga antibodies to epstein-barr virus capsid antigen preceding symptoms of nasopharyngeal carcinoma. | | 1978 | 75457 |
| transmission of non-a, non-b hepatitis from man to chimpanzee. | non-a, non-b hepatitis was transmitted to four colony-born chimpanzees by intravenous inoculation of human sera. two chimpanzees were inoculated with serum from a patient with a clinical and serological diagnosis of chronic non-a, non-b hepatitis whose blood appeared to transmit this disease to a nurse following accidental needle-stick, and the other two chimpanzees were inoculated with serum from either of two former blood-donors whose hbsag-negative blood appeared to transmit clinically recogn ... | 1978 | 76018 |
| non-a, non-b hepatitis. | | 1978 | 77141 |
| epstein-barr-specific iga in nasopharyngeal cancer. | | 1978 | 78104 |
| persistent epstein-barr virus infection in a child with hypergammaglobulinaemia and immunoblastic proliferation associated with a selective defect in immune interferon secretion. | a 5-year-old girl had a chronic disease characterised by fever, lymphoid hyperplasia, interstitial pneumonitis, thrombocytopenia, and polyclonal hypergammaglobulinaemia. evidence for severe, persistent epstein-barr virus (e.b.v.) infection was found: titres of antibody to e.b.v. viral capsid antigen (igm and igg) and early antigen were extremely high, cells containing e.b.v.-associated nuclear antigen (e.b.n.a.) were found in lymph nodes and blood, and spontaneous permanent lymphoblastoid cell l ... | 1978 | 79029 |
| epstein-barr virus and burkitt lymphoma. | | 1978 | 79925 |
| epstein-barr virus d.n.a. in tumour tissue from native alaskan patients with nasopharyngeal carcinoma. | | 1978 | 82107 |
| characterization of a leukemic cell line of the pre-b phenotype. | | 1979 | 83966 |
| epstein-barr virus infections in the x-linked recessive lymphoproliferative syndrome. | prospective studies demonstrated variable phenotypic expression of the x-linked recessive lymphoproliferative syndrome (x.l.r.l.s.) in three brothers: (1) hypogammaglobulinaemia and subclinical epstein-barr-virus (e.b.v.) infection with antibody response to e.b.v.; (2) e.b.v. infection with defective immune response to e.b.v., fatal infectious mononucleosis (i.m.), and immunoblastic lymphoma; and (3) histiocytic lymphoma. hypogammaglobulinaemia and measles pneumonitis had preceded infection with ... | 1978 | 85816 |
| cell-mediated immunity to epstein-barr virus in nasopharyngeal carcinoma. | | 1979 | 86139 |
| search for epstein-barr and type c oncornaviruses in systemic lupus erythematosus. | lymphoblastoid cell lines were derived from patients with active systemic lupus erythematosus by allowing spontaneous transformation of peripheral b lymphocytes (b cells) harboring endogenous epstein-barr virus or by superinfecting peripheral lymphocytes with exogeneous epstein-barr virus. results of extensive studies aimed at identifying type c oncornaviruses in these lymphoblastoid cells were entirely negative by electron microscopy, dna-dna hybridization, reverse transcriptase assays, and coc ... | 1979 | 88943 |
| [epstein-barr virus associated burkitt's lymphoma in a french child (author's transl)]. | pathologic diagnosis of malignant lymphoma, burkitt type, was made in a 12 year old child with an abdominal tumour. the association of this lymphoma with epstein-barr virus was done based on the detection of epstein-barr viral markers within the tumour cells, as well as on the patient's serology. to our knowledge, it is the first time that the association between burkitt's lymphoma and epstein-barr virus, which is frequent in africa, is reported in a caucasian french. | 1979 | 93275 |
| abortive expression of the epstein-barr virus (ebv) cycle in a variety of ebv dna-containing cell lines, as reflected by nucleic acid hybridization in situ. | a variety of epstein-barr virus (ebv) dna-containing cell lines have been tested for the expression of the ebv-associated antigens ebna (nuclear antigen), ea (early antigen), and vca (viral capsid antigen), and for the presence of cells containing disproportionate amounts of ebv dna. the antigen tests utilized immunofluorescence and 125i-labelled antibodies combined with autoradiography. ebv-dna was detected by in situ hybridization with 3h-labelled ebv rna complementary to p3hr-1 ebv dna (p-ebv ... | 1979 | 93584 |
| establishment of specific antibody producing human lines by antigen preselection and epstein-barr virus (ebv)-transformation. | specific antibody producing human cell lines were established by preselecting antigen binding b-lymphocytes and subsequently transforming ("immortalizing") them with epstein-barr virus (ebv). nnp-binding b-cells were isolated from the blood of three donors with high anti-nnp titers. ebv-transformation led to polyclonal cell lines that had 15-18% nnp receptor positive cells. a similar fraction of the cells produced nnp-specific plaques in the cunningham-szenberg assay. unconcentrated culture medi ... | 1979 | 95800 |
| herpesvirus papio: state and properties of intracellular viral dna in baboon lymphoblastoid cell lines. | herpesvirus papio (hvp) is an indigenous b-lymphotropic virus of baboons (papio sp.) present in latent form in baboon lymphoblastoid cell lines. it shares cross-reacting viral capsid and early antigens with the epstein-barr virus (ebv), and hvp dna and ebv dna show partial sequence homology. ebv-specific complementary rna was employed here as a probe to investigate the physical state of the hvp dna component in baboon lymphoblastoid cells after fractionation of cellular dna by density gradient c ... | 1979 | 113352 |
| induction of permanently proliferating human lymphoblastoid lines by n-methyl-n'-nitro-n-nitrosoguanidine. | n-methyl-n'-nitro-n-nitrosoguanidine treatment of human peripheral blood mononuclear cells increases the frequency of lymphocyte transformation into permanently proliferating lines. the transformation is dependent on the dose of n-methyl-n-7-nitro-n-nitrosoguanidine and does not occur in the presence of autologous human serum. thirty-eight of the forty-two established lines resulting fron n-methyl-n'-nitro-n-nitrosoguanidine treatment produced detectable epstein-barr virus antigens. | 1975 | 162872 |
| characterization of a serum inhibitor of mlc reactions. iii. specificity: hl-a-related antibodies. | | 1975 | 163145 |
| elevated immunofluorescence antibody titers to several herpesviruses in burkitt's lymphoma patients: are high titers unique? | antibody titers for viral capsid antigens of all four human herpesviruses were measured by immunofluorescence in the sera of 16 burkitt's lymphoma (bl) patients, 16 age-, sex-, and locality-matched controls, and 136 family members from the west nile district of uganda. among family members, titers greater than 1:4 were found in 98% for herpes simplex virus (hsv), 86% for varicella-zoster virus (vzv), 100% for cytomegalovirus (cmv), and 94% for epstein-barr (ebv). titers in patients averaged appr ... | 1975 | 163324 |
| dna of epstein-barr virus. i. comparative studies of the dna of epstein-barr virus from hr-1 and b95-8 cells: size, structure, and relatedness. | we have compared the properties of the dna of epstein-barr virus (ebv) purified from hr-1 (ebv hr-1 dna) and b95-8 (ebv b95-8 dna) continuous lymphoblast cultures. our data indicate that (i) the s suc of native ebv dna relative to t4d dna is 55s. using the modified burgi-hershey relationship (5), we estimate the molecular weight of native ebv dna is 101 (plus or minus the molecular weight of native fbv dna by measurement of the length of 3) times 106. estimation of the molecule relative to form ... | 1975 | 163373 |
| transfusion-associated hepatitis not due to viral hepatitis type a or b. | twenty-two patients who had an episode of transfusion-associated hepatitis not positive for hepatitis b antigen were examined for development of antibody to heaptitis a and b antigens, cytomegalovirus and epstein-barr virus. antibody response to the 27-nm virus-like hepatitis a antigen was measured by immune electron microscopy. in none of the 22 patients studied did serologic evidence of infection with hepatitis a virus develop during the study period. nine of the 22 patients had antibody respo ... | 1975 | 163436 |
| clinical evaluation of patients with infectious mononucleosis and development of antibodies to the r component of the epstein-barr virus-induced early antigen complex. | previous reports have emphasized that the transitory antibody responses to epstein-barr virus (ebv)-induced early antigens (ea) in the course of infectious mononucleosis are usually directed against the d (diffuse) component of the ea complex. in this report clinical and serologic data have been presented on 14 patients with infectious mononucleosis who responded either solely with antibodies to the r (restricted) component of the ea complex or revealed anti-r after the initial anti-d responses ... | 1975 | 163582 |
| epstein-barr virus binding sites on lymphocyte subpopulations and the origin of lymphoblasts in cultured lymphoic cell lines and in the blood of patients with infectious mononucleosis. | | 1975 | 163715 |
| an established burkitt's lymphoma line with cell membrane igg. | | 1975 | 163717 |
| classification and biological nature of established human hematopoietic cell lines. | over 200 established human hematopoietic cell lines of normal and malignant origin have been investigated by morphological and functional parameters. employing morphology as the overriding parameter four types of lines were identified. (1) lymphoblastoid cell lines, derived from normal and neoplastic hematopoietic tissue, were characterized by the wide morphologic flexibility of individual lymphoblastoid cells, constant association with epstein-barr virus (ebv), polyclonal derivation, differenti ... | 1975 | 165156 |
| clinical aspects of infection with the epstein-barr virus. | | 1975 | 165289 |
| epstein-barr virus genomes with properties of circular dna molecules in carrier cells. | a high-density fraction of high-molecular-weight dna was isolated from the human lymphoid cell line raji. this cell line contains 50 to 60 virus genome equivalents of epstein-barr virus dna per cell, and the high-density dna fraction was 10-fold enriched in such viral sequences. sedimentation analysis on neutral glycerol gradients, followed by hybridization experiments with viral complementary rna, showed that most of the intracellular viral dna sequences in this material did not cosediment with ... | 1975 | 165511 |
| cytotoxic effector cells specific for b cell lines transformed by epstein-barr virus are present in patients with infectious mononucleosis. | peripheral lymphoid cells, from 12 cases of acute infectious mononucleosis (im), were tested in a micro chromium-51 release assay for cytotoxic activity against a variety of cell lines that did or did not carry the epstein-barr virus (ebv) genome. unfractionated lymphocytes from these patients were cytotoxic to both types of cell lines, as were lymphocytes from healthy individuals. if, however, lymphocytes bearing complement receptors were removed, the residual im lymphocyte fraction was specifi ... | 1975 | 165518 |
| virus antibody levels in serum specimens from patients with optic neuritis and from matched controls;. | virus antibody levels in serum specimens taken in acute and convalescent phases from 77 patients with optic neuritits were tested by measles hamagglutination inhibition (hi), measles hemolysis inhibition (hli), rubella hi, parainfluenza-1 hi, epstein-barr immunofluorescence (if), and against 11 other viruses and mycoplasma pneumoniae with the complement fixation (cf) technique. the virus antibody levels were indicated to be usually very stable, and a fourfold change in virus antibody levels was ... | 1975 | 165652 |
| do viruses cause cancer in man? | | 1975 | 165866 |
| letter: infectious mononucleosis in children. | | 1975 | 166350 |
| [letter: burkitt's tumor. simultaneity in 2 sisters of ivory coast nationality]. | | 1975 | 166354 |
| viral and cellular dna synthesis in nuclei from human lymphocytes transformed by epstein-barr virus. | a dna-synthesizing system in vitro, using nuclei prepared by treatment of human lymphocytes with the detergent brij 58, was developed. nuclei from cultured lymphocytes synthesized dna for as long as 5 hr, and required atp, deoxynucleoside triphosphates, magnesium, and a calcium chelator. in nuclei from a partially synchronized line of cultured lymphocytes carrying several hundred copies of the epstein-barr viral genome, synthesis in vitro was predominately viral in early s phase and cellular in ... | 1975 | 166387 |
| epstein-barr virus: comparison of different strains for their biological activities in vitro. | several sources of epstein-barr virus (ebv) were tested for the ability to superinfect raji cells or to transform human cord blood leukocytes. ebv prepared from p3hr-1 cells showed only the ability to superinfect raji cells whereas ebv from qimr-wil cells from a cell line (cru-l1) derived from an infectious mononucleosis (im) patient or virus from throat washings from im patients transformed human cord blood leukocytes, but did not superinfect raji cells. | 1975 | 166422 |
| newer knowledge in comparative virology--its contribution to human health research. | like other comparative sciences, and despite its recent beginning comparative virology has already contributed useful applications and observations to human health research. teachings derived from the study of marek's disease found application in that of burkitt's lymphoma, and may lead to a possible vaccine against the human disease. equally useful information came from the study of canine distemper in the development of a chorio-allantoic membrane attenuated measles vaccine, and in our knowled ... | 1975 | 167504 |
| epidemiological studies of epstein-barr herpesvirus infection in western australia. | in a study of a caucasian population in western australia the prevalence of antibodies to epstein-barr virus (ebv) was 41% in the 9- to 10-year age group, 80% in the 16 to 19-year age group and 92% in young adults. the age-specific annual seroconversion rates indicated two peaks of primary ebv infection in the population studied - one under 5 years of age and the other at adolescence. the geometric mean titre rose with age, from 23 at 5-6 years to 53 at 36-40 years. it was shown that in 73 famil ... | 1975 | 168249 |
| letter: interpersonal transmission of eb-virus infection. | | 1975 | 168494 |
| letter: immunodiffusion studies of lymphoblast cell antigens. | | 1975 | 169368 |
| editorial: leukaemia in animals and man. | | 1975 | 169460 |
| parallel tubular structures in lymphocytes. ii. correlation with cellular immunity and cytomegalovirus and epstein-barr virus antibodies in hodgkin's disease. | the increased incidence of parallel tubular structures in lymphocytes of patients with hodgkin's disease was investigated for a correlation with either impairment of cellular immunity (measured by dncb-skin test and pha-induced lymphocyte stimulation in vitro) or an increase of antibodies against cytomegalovirus or epstein-barr virus. no correlations were found. statistical analysis revealed antibody titers especially in the group of patients with high percentages of lymphocytes containing the t ... | 1975 | 169660 |
| immunology of sarcoidosis. | the cardinal immunologic changes in sarcoidosis consist of depression of delayed-type hypersensitivity, hyperreactive circulating antibody responses and the kveim-siltzbach skin test phenomenon. depression of delayed-type hypersensitivity is demonstrated by skin tests using tuberculin, mumps, pertussis, trichophytin, oidiomycin, dinitrochlorobenzene and californian keyhole limpet hemocyanin. the cultured lymphocytes from patients with depression of delayed-type hypersensitivity react poorly to p ... | 1975 | 169693 |
| experiments to vaccinate marmoset monkeys against malignant lymphoma. | humoral antibodies can be induced in cotton topped (ct) marmosets with killed herpesvirus saimiri (hvs) vaccines. the serum antibodies delayed, but did not prevent, malignant lymphoma in actively and passively immunized monkeys after inoculation with 10(3.8)tcid50 of cell-free hvs. experiments are in progress to determine whether the vaccines are able to protect against smaller challenge dosages of hvs. the immunized monkeys were not resistant against transplantation of tumor cells. infection wi ... | 1975 | 169814 |
| detection of epstein-barr virus dna in human tumors. | the presence of epstein-barr virus (ebv) dna in biopsies of human tumors was tested by complementary rna (crna) hybridization on membranes and dna-dna reassociation kinetics. dna-dna reassociation kinetics can detect as few as 1 ebv genome in every 50 cells, and the crna method can detect ebv genomes if 2 or more are present in a cell. twenty-three out of 24 african burkitt's lymphoma biopsies, 18 out of 23 african nasopharyngeal carcinomas (npc), and 8 out of 24 other african tumors were positi ... | 1975 | 169825 |
| dual persistence of epstein-barr viral and type-c viral genomes in nonproducer human lymphoblastoid cells. | | 1975 | 169826 |
| editorial: the several viruses of post-transfusion hepatitis. | | 1975 | 169948 |
| oncogenic properties of human viruses. | | 1975 | 170197 |
| ultraviolet inactivation of epstein-barr virus: effect on synthesis of virus-associated antigens. | the relative sensitivity to ultraviolet (uv) light of genome functions of the p3hr-1 strain of epstein-barr virus (ebv) was studied. the formation of viral capsid antigen (vca) appeared to be more sensitive than that of early antigen (ea), while the synthesis of membrane antigen (ma) was most resistant, as seen on examination in the presence of cytosine arabinoside (ara-c). however, the appearance of both vca and ea, but not that of ma, was delayed with uv-irradiated virus, in either the presenc ... | 1975 | 170209 |
| sensitivity of the epstein-barr virus transformed human lymphoid cell lines to interferon. | the effect of interferon on expression of epstein-barr virus (ebv) early gene functions was investigated. the 'early antigen' synthesis which follows either ebv superinfection of established lymphoid cell lines or 5'-iododeoxyuridine activation of the intrinsic ebv genomes harboured by these cells could be suppressed with interferon. in contrast, the spontaneous early antigen expression that occurs in a few per cent of the cells in the producer cell lines could not be blocked with interferon. th ... | 1975 | 170370 |
| epstein-barr virus antibodies in patients with carcinoma of the nasopharynx and carcinoma of other sites in the head and neck. | serum antibody titers to epstein-barr virus were significantly elevated in a group of twenty-three patients with nasopharyngeal carcinoma as compared with eighty-six patients with cancer of other sites in the head and neck and 222 age-matched controls. our findings in this group of patients support the suggestion of an association between the epstein-barr virus and nasopharyngeal carcinoma. | 1975 | 170838 |
| nasopharyngeal carcinoma. ix. antibodies to ebna and correlation with response to other ebv antigens in chinese patients. | ninety-five sera from chinese npc patients in different stages of the disease, 38 sera from chinese patients with other cancers, and 50 normal chinese sera, were titrated for ebna, vca, ea and complement-fixing (cf/s) ebv-specific antibodies. the geometric mean (gmt) ebna antibody titre of patients with npc at stage i was found to be four times higher than that of normal individuals and increased in parallel with clinical deterioration. antibody titres against ebna did not correlate with either ... | 1975 | 171226 |
| a prospective evaluation of heterophile and epstein-barr virus-specific igm antibody tests in clinical and subclinical infectious mononucleosis: specificity and sensitivity of the tests and persistence of antibody. | three heterophile antibody tests and a test specific for igm antibody to epstein-barr virus were evaluated during prospective studies of infectious mononucleosis. specificity was judged by the frequency of false-positive results in sera of known qualities taken before illness; except for two patients bled during early, unrecognized illnes,, titers of greater than or equal to 1:40 were detected in 12% by the absorbed sheep red cell test, in 6.7% by the absorbed horse red cell test, and in none by ... | 1975 | 171321 |
| hbs-ag-negative hepatitis in a hemodialysis unit: relation to epstein-barr virus. | eleven of 40 patients in a hemodialysis unit had clinical or biochemical evidence of hepatitis during a five-week period. the clinical disease was mild, being limited solely to dialysis patients. epidemiologic investigation indicated that the incubation period was between 17 and 35 days and that 10 of 11 patients had been exposed to a single venous-pressure monitor before onset. dried blood and evidence of blood reflux up the venous-pressure gauge suggested that cross-contamination of the blood ... | 1975 | 171573 |
| the epstein-barr virus and neoplasia. | | 1975 | 171578 |
| etiological spectrum of post-transfusion hepatitis. | frequent occurrence of post-transfusion hepatitis continues despite screening for australia antigen in blood before transfusion and elimination of commercial donor sources. the majority of these cases appears unrelated to hepatitis b virus. preoperative, acute, and convalescent serra were screened for evidence of hepatitis b, hepatitis a, epstein-barr, and cytomegalovirus exposure in 34 cardiac surgery patients developing post-transfusion hepatitis postoperatively. four patients showed evidence ... | 1975 | 172400 |
| origin of lymphoid lines established from mixed cultures of cord-blood lymphocytes and explants from infectious mononucleosis, burkitt lymphoma and healthy donors. | lymphocytes were explanted from ebv-seropositive donors including peripheral blood of infectious mononucleosis patients, healthy donors and ebv-genome-carrying cells from burkitt lymphoma (bl) biopsies or nude mouse-passaged, bl-biopsy-derived lines. the explanted cells were mixed with fresh cord-blood lymphocytes from mice of the opposite sex. in all categories of derived lines, cord-blood cell progeny was predominant, as judged by the sex marker and other associated markers. only one bl biopsy ... | 1975 | 172457 |
| sensitive method to detect non-complement-fixing antibodies to epstein-barr virus nuclear antigne. | the anti-complement immunofluorescence (acif) test was modified to detect non-complement-fixing antibodies to epstein-barr (eb) virus nuclear antigen (ibna). these ebna antibodies belong to the immunoglobulin classes iga and igg. in our method anti-human gamma-globulin (ahg) was bound to the ebna antibodies before the complement was added. if only non-complement-fixing antibodies are present the complement can be fixed to the ahg. within only a few weeks of the onset of infectious mononucleosis ... | 1975 | 172458 |
| virologic studies in rheumatoid arthritis. | comparison of virus antibody titers in jra and matched controls failed to implicate rubella, measles, parainfluenza type 1 or epstein-barr viruses. neither rna viruses or mycoplasma were detected in rheumatoid synovial cell cultures by 3h-uridine incorporation. mycoplasma were not isolated from rheumatoid synovial membranes or cell cultures using the large specimen-broth culture procedure. the balance of available evidence, including our own, suggests that mycoplasma are not involved in ra. viru ... | 1975 | 173010 |
| letter: acute leukemia with burkitt's tumor cells. | | 1975 | 173442 |
| anti-epstein-barr virus antibody in patients with cancer of various sites and control groups. | sera collected from patients with nasopharyngeal carcinoma (npc, 321 cases), cancer of the other sites (297 cases), diseases of the ear, nose and throat (64 cases) and neighborhood controls matched for age and sex (817 cases) were titrated for antibodies to epstein-barr virus (ebv) by the indirect immunofluorescence antibody technique. high anti-ebv antibody titers (greater than or equal to 1:640) were noted in 55% in patients with npc but only less than 31% were noted for the other 3 groups. th ... | 1975 | 173499 |
| viral-induced thymidine kinase isozymes. | | 1975 | 174152 |
| [oncogenic viruses and carcinogenesis in man]. | | 1975 | 174160 |
| [different types of oncogenic viruses associated with hemoblastosis in monkeys]. | | 1975 | 174875 |
| [antibodies against the epstein-barr virus, capsid antigen in children with malignant neoplasms (author's transl)]. | sera from children were tested for antibodies to viral capsid antigens of epstein-barr virus. the method applied was the indirect immunofluorescence test. 25 sera from healthy donors were titrated as controls. in 14 patients with acute lymphocytic leukemia we found low levels, a tiny little girl with chronic myelocytic leukemia (?) and three children.with lymphoproliferative disorders showed highly increased serological reactivities. investigated children with other kinds of tumours did not sero ... | 1976 | 175342 |
| [production of interferon or interferon-like susbstances in eb3 cultures after inoculation with human serum containing australia antigen]. | | 1975 | 175431 |
| [epidemiology in the study of the role of viruses in human cancers]. | an epidemiological approach is very useful in the study of the role of viruses in the development of human tumors, if properly integrated with the experimental approach and laboratory studies. certain epidemiological characteristics of human tumours favour a viral etiology, e.g. space and time clustering, vertical and horizontal transmission of a tumour risk, succession in space or in time of viral induced benign lesions and of malignant tumours. in the framework of the association between the e ... | 1975 | 175869 |
| cell-killing by epstein-barr virus: analysis by colony inhibition procedure. | the p3hr-1 and b95-8 strains of epstein-barr virus (ebv) were cytocidal for ebv-carrier human cell lines, as revealed by a colony inhibition procedure. the cytocidal activity was proportional to virus dose added. the cell killing was neutralized by anti-ebv antibody-positive but not -negative human sera. when the relative sensitivity to ultraviolet light of ebv activities was examined, the cytocidal actitivy was much more resistant than the viral infectivity as assayed by early antigen-forming a ... | 1976 | 176118 |
| epstein-barr virus--is it time to develop a vaccine program? | | 1976 | 176406 |
| epstein-barr virus in an american patient with burkitt's lymphoma: detection of viral genome in tumor tissue and establishment of a tumor-derived cell line (nab). | epstein-barr virus (ebv) dna (17.7 genome equivalents/cell) was found in tumor tissue from an american patient with burkitt's lymphoma who had never traveled outside the united states. a lymphoid cell line (nab) containing the ebv genome was established from tumor tissue from this patient; characteristics of this cell line were described. previous burkitt's tumors found in americans and examined by molecular hybridization were negative for ebv dna. our results suggested that ebv is associated wi ... | 1976 | 176407 |
| expression of epstein-barr-virus-associated membrane antigen in raji cells superinfected with two different virus strains. | | 1976 | 176804 |
| facial diplegia. | sudden facial diplegia or recurrent facial palsy is a rare occurrence, and may be the only symptom indicative of a systemic illness. two patients had this condition in the absence of guillain-barré-strohl syndrome. | 1976 | 178296 |
| establishment and characterization of a human histiocytic lymphoma cell line (u-937). | a human hematopoietic cell line (u-937) with exceptional characteristics was derived from a patient with generalized histiocytic lymphoma. the morphology of the cell line was identical to that of the tumor cells in the pleural effusion from which the line was derived. since epstein-barr virus (ebv) carrying diploid lymphoblastoid cell lines unrelated to the tumor population often become established in vitro from non-burkitt lymphoma explants, several parameters were studied to discriminate the u ... | 1976 | 178611 |
| diseases associated with herpesviruses. | human herpesviruses have been associated with numerous diseases throughout history (table 3), but their ability to induce latent and recurrent infections, alongwith their oncogenic capabilities, is only beginning to be understood. accordingly, our ability to deal with the diseases induced by herpesviruses is severely limited by our lack of information concerning the basic processes of virus-host cell interactions and systemic host factors; especially immune factors that are involved in the disea ... | 1976 | 179753 |
| differential induction of epstein-barr virus-related antigens in human lymphoblastoid cells by virus-mediated cell-to-cell contact. | differential induction of epstein-barr virus (ebv)-related antigens was observed after sendai virus-medicated fusion of producer and non-producer cells with various human and mouse cells. the ebv-determined early nuclear antigen (ena), early antigen (ea) and viral capsid antigen (vca) could be induced at a high rate in producer cells (p3hr-1 and b95-8), normally expressing these antigens at very low frequency, as early as 12-24 h after fusion with each other or with human amnion fl cells. in con ... | 1976 | 179955 |
| immunological control of virus-induced tumors in primates. | cells infected by oncogenic viruses may transform, may develop a latent carrier state, or may be destroyed but understanding of the control of the results of infection is incomplete. even if cells transform, ultimate development of a tumor may be immunologically controlled. for example, cells of some marmoset species transform after infection with rna tumor viruses, and animals react to the transformed cells with cell-mediated and humoral immune responses. both virus specific and cross-reacting ... | 1975 | 169832 |
| detection of epstein-barr virus dna in american burkitt's lymphoma. | twenty american patients with burkitt's lymphoma (bl) were studied serologically and biochemically to detect an association with epstein-barr virus (ebv). a wide range of antibody reactivities was encountered. five patients had serological profiles similar to those of african patients with bl: one and possibly two of these patients harbored ebv-dna in their tumors. fifteen patients had low or absent antibody to ebv: tumors from 10 of these patients lacked evidence of ebv-dna. an unexplained asso ... | 1976 | 181331 |
| an ebv-genome-negative cell line established from an american burkitt lymphoma; receptor characteristics. ebv infectibility and permanent conversion into ebv-positive sublines by in vitro infection. | an in vitro line was derived from an american burkitt lymphoma, designated ra no. 1, which produced malignant tumors when inoculated into thymus-deficient nude mice. the cells have b-lymphocyte characteristics, with surface-associated mu and kappa chains and epstein-barr virus (ebv) receptors, and can be readily infected with ebv in vitro. b95-8 virus induced ebv-determined nuclear antigen (ebna) but not early antigen (ea) in ra no. 1 cells, whereas p3hr-1 virus induced both ebna and ea, but the ... | 1975 | 181343 |
| [human cancer and herpesviruses. eb virus. immunological studies of nasopharyngeal cancer]. | | 1976 | 181611 |
| virus antibody levels and delayed hypersensitivity in rheumatoid arthritis. | epstein-barr virus and cytomegalovirus antibody levels were not higher in patients with rheumatoid arthritis compared to matched controls. delayed hypersensitivity, measured by skin test reactivity, was depressed in rheumatoid arthritis. there was no correlation between virus antibody titres and delayed hypersensitivity. | 1976 | 182092 |
| herpes simplex-raji(a44), a new cell line for serologic testing by immunofluorescence. | an if technique for the detection of hsv antibodies by using a lymphoblastoid cell line, raji(a44), which continuously produce a constant amount of hsv antigen, is described. if and ih tests were found to be similar with respect to sensitivity and reproducibility. sero-conversions detected by the cf test were detected with this cell line. the raji(a44) line (cells in suspension) can be routinely used for the diagnosis of hsv infection. | 1976 | 182883 |
| epstein-barr virus associated with episodes of recurrent tonsillitis. | a group of patients with a history of recurrent tonsillitis were observed during an acute episode to determine the cause of the infection. the microbial pathogen that was consistently implicated was the epstein-barr virus. seventeen (65%) of 26 patients exhibited a substantial seroconversion to the early antigen of epstein-barr-virus-infected lymphoblastoid cells (p3hr-1). we conclude that there is a high incidence of tonsillitis associated with the epstein-barr virus. the propensity of the viru ... | 1975 | 169772 |
| leukocyte transforming agent (epstein-barr virus) in newborn infants and older individuals. | the lymphocyte transforming agent, associated with epstein-barr virus, was sought in the oropharynx and other clinical sites of 443 individuals in the following groups: premature and term neonates; infants with congenital malformations or with suspected torch syndrome; children with various illnesses; pregnant and postpartum women; healthy adults; and patients with infectious mononucleosis. evidence of intrauterine infection was found in one newborn infant and lta was demonstrated in a 16-day-ol ... | 1976 | 182946 |
| acute leukemia in burkitt lymphoma. | burkitt lymphoma uncommonly presents as a acute leukemia. we describe the clinical course and findings of a 14-year-old female with burkitt lymphoma who presented with acute leukemia. splenomegaly and an abdominal mass. she responded initially to prednisone alone and later achieved full remission with combination chemotherapy. established morphologic criteria and clinical course were consistent with the diagnosis of burkitt lymphoma (1), although b-cell determinants were not present on her tumor ... | 1976 | 183092 |
| transformation of human lymphocytes by epstein-barr virus is inhibited by phosphonoacetic acid. | | 1976 | 183149 |
| epstein-barr virus dna in human lymphoid cell lines: in vitro conversion. | | 1976 | 183361 |