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tick cell lines for study of crimean-congo hemorrhagic fever virus and other arboviruses.abstract continuous cell lines derived from many of the vectors of tick-borne arboviruses of medical and veterinary importance are now available. their role as tools in arbovirus research to date is reviewed and their potential application in studies of tick cell responses to virus infection is explored, by comparison with recent progress in understanding mosquito immunity to arbovirus infection. a preliminary study of propagation of the human pathogen crimean-congo hemorrhagic fever virus (cc ...201121955214
co-circulation of crimean-congo hemorrhagic fever virus strains asia 1 and 2 between the border of iran and pakistan.crimean-congo hemorrhagic fever (cchf) is a tick-borne viral disease that is transmitted by numerous species of ticks, which serve both as a reservoir and vector of cchf virus (cchfv). molecular and serological tests were undertaken on hard ticks (ixodidae spp.) and samples from livestock were collected in 2015 from chabahar county in southeast iran. using rt-pcr, the ticks were tested for the presence of cchfv. in addition, seven livestock were serologically tested for the presence of igg antib ...201729159319
genetic characterization and molecular clock analyses of the crimean-congo hemorrhagic fever virus from human and ticks in india, 2010-2011.a nosocomial outbreak of crimean congo hemorrhagic fever (cchf) was reported among humans in ahmadabad district, gujarat, india during january, 2011. in the present study we provide the complete genomic sequences of four cchfv isolates derived from two human patients and two pools of hyalomma anatolicum ticks during the period of this outbreak and the complete s segment sequence of two retrospective human serum samples, positive for cchfv in 2010. sequence-based molecular characterization of the ...201323195573
[genetic variants of the crimean-congo hemorrhagic fever virus circulating in endemic areas of the southern tajikistan in 2009].506 hyalomma anatolicum ticks were collected and assayed in two crimean-congo hemorrhagic fever (cchf) endemic regions of tajikistan. antigen and rna of cchf virus were detected in 3.4% of tick pools from rudaki district using elisa and rt-pcr tests. as of tursunzade district, viral antigen was identified in 9.0% of samples and viral rna was identified in 8.1% of samples. the multiple alignment of the obtained nucleotide sequences of cchf virus genome s-segment 287-nt region (996-1282) and multi ...201324364143
crimean-congo hemorrhagic fever virus clade iv (asia 1) in ticks of western iran.crimean-congo hemorrhagic fever virus (cchfv) is transmitted through the bite of an infected tick, or by direct contact with cchfv-infected patients' blood or the products of infected livestock. in 2012, ticks were collected in eight regions of lorestan province, iran. in total, 434 ticks were collected. reverse transcriptase polymerase chain reaction was used for the detection of cchfv rna. of 434 ticks, 419 (96.6%) ticks were from the family ixodidae (hard ticks) and 15 (3.5%) ticks were from ...201526336221
molecular detection of crimean-congo haemorrhagic fever virus in ticks collected from infested livestock populations in a new endemic area, south of iran.crimean-congo haemorrhagic fever (cchf) is a viral zoonotic disease with potentially fatal systemic effects on man. we aimed to determine the presence of cchf virus among collected ticks from domestic livestock from october 2012 to september 2013.201626758985
detection, isolation and confirmation of crimean-congo hemorrhagic fever virus in human, ticks and animals in ahmadabad, india, 2010-2011.in january 2011, human cases with hemorrhagic manifestations in the hospital staff were reported from a tertiary care hospital in ahmadabad, india. this paper reports a detailed epidemiological investigation of nosocomial outbreak from the affected area of ahmadabad, gujarat, india.201222616022
Crimean-Congo hemorrhagic fever virus in various ixodid tick species from a highly endemic area.Ticks are major vectors of numerous diseases affecting animals and humans. Presence of various tick-borne pathogens such as Crimean-Congo hemorrhagic fever virus (CCHFV) in various tick species was documented. CCHF is a severe tick-borne illness caused by the CCHFV which is a member of the Nairovirus genus (family: Bunyaviridae). Presence of CCHFV was shown in the most prevalent ixodid tick species such as Hyalomma marginatum and Rhiphicephalus bursa in Turkey. In the present study, prevalence a ...201122119389
genetic analysis of the m rna segment of crimean-congo hemorrhagic fever virus strains in turkey.crimean-congo hemorrhagic fever (cchf) virus is member of the genus nairovirus of the family bunyaviridae. all members of the family bunyaviridae are enveloped viruses containing tripartite, negative polarity, single-stranded rna. cchf is characterized by high case mortality, occurring in asia, africa, the middle east and europe. during recent years, outbreaks have been reported in turkey. however, little information is available on the genetic diversity of cchf virus in turkey. in this study, a ...200817955162
[genetic monitoring of the crimean-congo hemorrhagic fever virus in kazakhstan and tajikistan in 2001-2003].blood specimens obtained from 32 cchf patients were tested for the presence of cchf virus markers. in addition, 3210 ticks of the genera hyalomma asiaticum, hyalomma anatolicum, and dermacentor niveus were examined to identify the cchf virus antigen and rna. this material was obtained during the 2001-2003 local outbreaks of cchf in kazakhstan and tajikistan. the nucleotide sequence in the region 983-1282 of s segment of the cchf virus for 12 wild type strains was determined. the phylogenetic rel ...200616756002
crimean-congo hemorrhagic fever: a molecular survey on hard ticks (ixodidae) in yazd province, iran.to determine the rate of crimean-congo hemorrhagic fever virus (cchfv) infection in hard ticks (ixodidae) in yazd province of iran.201121771418
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